##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550668_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	705992
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52320847828304	33.0	31.0	34.0	31.0	34.0
2	32.664480617344104	34.0	31.0	34.0	31.0	34.0
3	32.62529462090222	34.0	31.0	34.0	31.0	34.0
4	36.09012283425308	37.0	35.0	37.0	35.0	37.0
5	36.15827799748439	37.0	35.0	37.0	35.0	37.0
6	36.20234365261929	37.0	37.0	37.0	35.0	37.0
7	36.22501388117713	37.0	37.0	37.0	35.0	37.0
8	36.16276388401002	37.0	37.0	37.0	35.0	37.0
9	38.02304983625877	39.0	38.0	39.0	35.0	39.0
10	37.783195843578966	39.0	38.0	39.0	35.0	39.0
11	37.69855465784315	39.0	37.0	39.0	35.0	39.0
12	37.66127944792576	39.0	37.0	39.0	35.0	39.0
13	37.67122290337568	39.0	37.0	39.0	35.0	39.0
14	39.01341799907081	40.0	38.0	41.0	35.0	41.0
15	39.09388633298961	40.0	39.0	41.0	35.0	41.0
16	39.11733135786241	40.0	39.0	41.0	35.0	41.0
17	39.06726138539813	40.0	38.0	41.0	35.0	41.0
18	38.99649854389285	40.0	38.0	41.0	35.0	41.0
19	38.93682364672687	40.0	38.0	41.0	35.0	41.0
20	38.87534844587474	40.0	38.0	41.0	35.0	41.0
21	38.806248512731024	40.0	38.0	41.0	35.0	41.0
22	38.76975518136183	40.0	38.0	41.0	35.0	41.0
23	38.73755651622115	40.0	38.0	41.0	35.0	41.0
24	38.72153367176965	40.0	38.0	41.0	35.0	41.0
25	38.64504980226405	40.0	38.0	41.0	35.0	41.0
26	38.646003070856324	40.0	38.0	41.0	35.0	41.0
27	38.63414741243527	40.0	38.0	41.0	35.0	41.0
28	38.571502226654125	40.0	38.0	41.0	35.0	41.0
29	38.53849335403234	40.0	38.0	41.0	35.0	41.0
30	38.45790745504198	40.0	38.0	41.0	35.0	41.0
31	38.3899095173883	40.0	38.0	41.0	34.0	41.0
32	38.27300450996612	40.0	38.0	41.0	34.0	41.0
33	38.19168064227357	40.0	38.0	41.0	34.0	41.0
34	38.05912248297431	40.0	37.0	41.0	34.0	41.0
35	37.98600267425127	40.0	37.0	41.0	34.0	41.0
36	37.92710115695362	40.0	37.0	41.0	34.0	41.0
37	37.888470407596685	40.0	37.0	41.0	34.0	41.0
38	37.82386202676518	40.0	37.0	41.0	34.0	41.0
39	37.753653015898195	40.0	37.0	41.0	33.0	41.0
40	37.6670231390724	40.0	37.0	41.0	33.0	41.0
41	37.61190778365761	40.0	36.0	41.0	33.0	41.0
42	37.560302383029835	40.0	36.0	41.0	33.0	41.0
43	37.51521263697039	40.0	36.0	41.0	33.0	41.0
44	37.44717787170393	40.0	36.0	41.0	33.0	41.0
45	37.35147565411506	40.0	36.0	41.0	33.0	41.0
46	37.27076085847998	40.0	35.0	41.0	33.0	41.0
47	37.207979693820896	40.0	35.0	41.0	33.0	41.0
48	37.164863057938334	40.0	35.0	41.0	33.0	41.0
49	37.11412735555077	39.0	35.0	41.0	33.0	41.0
50	37.02573541909823	39.0	35.0	41.0	32.0	41.0
51	36.16069162256796	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	16.0
10	16.0
11	9.0
12	11.0
13	13.0
14	15.0
15	17.0
16	39.0
17	57.0
18	78.0
19	169.0
20	279.0
21	459.0
22	745.0
23	1134.0
24	1832.0
25	2967.0
26	4308.0
27	5494.0
28	6034.0
29	6171.0
30	7087.0
31	8579.0
32	10960.0
33	15083.0
34	30217.0
35	44926.0
36	40043.0
37	61834.0
38	124181.0
39	332751.0
40	461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.12695327992385	21.71979285884259	24.690081474011034	12.463172387222517
2	32.19101632879693	24.67251753560947	26.006526986141488	17.12993914945212
3	31.98676472254643	23.715707826717583	27.888276354406283	16.4092510963297
4	28.621570782671757	25.723379301748462	27.11050550147877	18.54454441410101
5	25.170398531428116	30.381222450112748	25.38116579224694	19.067213226212196
6	26.132449092907567	34.90407823318111	23.80876837131299	15.15470430259833
7	81.44709855069179	5.770320343573299	8.51624381012816	4.26633729560675
8	83.07119627417875	5.21549819261408	7.966945801085565	3.7463597321216104
9	76.25143061111174	8.177429772575326	10.129434894446396	5.441704721866537
10	37.299856089020835	33.77077927228637	16.23247855499779	12.696886083695
11	26.57013110630149	24.95694002198325	29.4885777742524	18.984351097462863
12	25.35099547870231	23.272218382077984	31.27103423268252	20.105751906537183
13	24.15820575870548	23.35479722149826	32.26452990968736	20.222467110108898
14	20.24102256116217	27.26022391188569	30.81196387494476	21.686789652007388
15	20.30872871080692	27.676092646942173	32.387335833833816	19.627842808417093
16	23.78992963093066	26.406106584777167	30.70275583859307	19.1012079456991
17	23.334825323799706	26.926225792926832	28.503439132454762	21.235509750818707
18	23.03878797493456	27.189826513614886	29.194806740019718	20.576578771430835
19	24.00777912497592	27.853573411596734	28.04238574941359	20.096261714013757
20	25.063881743702478	27.770711282847397	28.467886321658035	18.69752065179209
21	24.83923330575984	27.21163979195232	29.034323335108613	18.91480356717923
22	23.48312728756133	24.95722331131231	29.329227526657526	22.230421874468835
23	22.474050697458328	26.26559507756462	30.046799397160306	21.213554827816747
24	22.650256660132126	25.94307017643259	29.752461784269514	21.65421137916577
25	22.50521252365466	27.509518521456332	28.40159661865857	21.58367233623044
26	21.436078595791454	28.88417999070811	28.20697684959603	21.472764563904406
27	22.090760235243458	27.585581706308286	29.25585559043162	21.067802468016637
28	20.225866582057588	27.980487031014516	30.2977937427053	21.495852644222595
29	21.936225906242562	27.27283028702875	28.592533626443362	22.19841018028533
30	22.26427494929121	26.493926276785007	29.84764699883285	21.394151775090936
31	23.108902083876302	27.051722965699327	28.867324275629187	20.97205067479518
32	22.470934514838696	27.159372910741197	28.959818241566477	21.40987433285363
33	22.56045394282088	26.712483994152908	28.521286360185382	22.205775702840825
34	21.029841697922922	26.511773504515634	30.38249725209351	22.075887545467936
35	21.40392525694342	26.34434951104262	28.80868338451427	23.44304184749969
36	21.280977688132445	27.678358961574634	28.732336910333263	22.30832643995966
37	21.588488254824416	27.283028702874816	29.41690557400084	21.711577468299925
38	20.973750410769526	27.32962979750479	27.642522861448853	24.05409693027683
39	22.765697061723078	25.229039422543032	27.938135276320413	24.067128239413478
40	22.620369635916553	25.634851386417978	29.707418780949357	22.03736019671611
41	20.062125349862324	26.456107151355823	29.077666602454418	24.40410089632744
42	20.855193826558942	27.3075332298383	28.410945166517465	23.426327777085294
43	21.76766875545332	25.23654658976306	29.241266189985154	23.75451846479847
44	21.633106324150983	26.182308014821697	28.57326995206745	23.611315708959875
45	21.879001461772937	25.164591100182438	28.443948373352672	24.512459064691953
46	21.476305680517626	26.304122426316447	28.57270337340933	23.646868519756598
47	20.839187979467187	25.683718795680406	30.166489138687126	23.31060408616528
48	20.297963716302732	24.98243606159843	30.172296569932804	24.54730365216603
49	20.889613480039433	25.207226144205602	29.912661899851557	23.99049847590341
50	19.681809425602555	24.95948962594477	30.54892973291482	24.809771215537854
51	20.688053122414985	24.479739147185803	29.095655474849575	25.73655225554964
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	201.0
1	530.5
2	860.0
3	3571.5
4	6283.0
5	4165.0
6	2047.0
7	2015.0
8	1983.0
9	1918.5
10	1854.0
11	1835.5
12	1817.0
13	1799.5
14	1782.0
15	1724.5
16	1667.0
17	1568.5
18	1470.0
19	1627.0
20	1784.0
21	2041.0
22	2298.0
23	2766.5
24	3235.0
25	4069.0
26	6098.0
27	7293.0
28	9236.5
29	11180.0
30	12597.0
31	14014.0
32	16150.5
33	18287.0
34	20324.0
35	22361.0
36	23590.5
37	24820.0
38	26769.5
39	28719.0
40	32785.0
41	36851.0
42	39545.0
43	42239.0
44	45310.0
45	48381.0
46	53770.5
47	59160.0
48	62155.5
49	65151.0
50	62330.0
51	59509.0
52	53971.0
53	48433.0
54	43552.5
55	38672.0
56	35817.0
57	32962.0
58	31252.5
59	29543.0
60	28467.0
61	27391.0
62	24745.5
63	22100.0
64	17890.5
65	13681.0
66	11428.5
67	9176.0
68	7359.0
69	5542.0
70	4545.0
71	3548.0
72	2838.0
73	2128.0
74	1724.0
75	1070.5
76	821.0
77	558.0
78	295.0
79	197.5
80	100.0
81	77.0
82	54.0
83	51.5
84	49.0
85	35.5
86	22.0
87	13.0
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	705992.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.21007447978293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.12656265819118	21.886552435733112
2	8.480234326197822	5.2933748985329165
3	3.348779285326887	3.1354695273421913
4	2.1476064580316057	2.6810783003371688
5	1.5834422253846028	2.470967489434334
6	1.3687356667596529	2.5631005261622466
7	1.2726037959762726	2.780264147897176
8	1.1241525688685852	2.806790832082228
9	1.0441077757531958	2.9328013301559963
>10	9.397810743156892	48.70276163065315
>50	0.0686722701855467	1.442233947163245
>100	0.03319917695423392	2.0555488644239825
>500	0.003183482721638869	0.6100503396833502
>1k	9.095664918968197E-4	0.6390057303988931
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3480	0.49292343256014237	No Hit
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1022	0.14476084714840962	TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	720	0.10198415846071911	TruSeq Adapter, Index 22 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4164466452877653E-4	0.0	0.0	0.09603508255051049	0.0
2	1.4164466452877653E-4	0.0	0.0	0.4424979319878979	0.0
3	1.4164466452877653E-4	0.0	0.0	0.7083649673084115	0.0
4	1.4164466452877653E-4	0.0	0.0	1.0617684053077088	0.0
5	1.4164466452877653E-4	0.0	0.0	2.0406746818660833	0.0
6	1.4164466452877653E-4	0.0	0.0	2.8486158483382247	0.0
7	1.4164466452877653E-4	0.0	0.0	3.4758184228716473	0.0
8	1.4164466452877653E-4	0.0	0.0	4.4086901834581695	0.0
9	1.4164466452877653E-4	0.0	0.0	4.827391811805233	0.0
10	1.4164466452877653E-4	0.0	0.0	5.576975376491518	0.0
11	1.4164466452877653E-4	0.0	0.0	6.546674749855522	0.0
12	1.4164466452877653E-4	0.0	0.0	7.3039071264263615	0.0
13	1.4164466452877653E-4	0.0	0.0	7.635497286088228	0.0
14	2.8328932905755306E-4	0.0	0.0	7.800654964928781	0.0
15	2.8328932905755306E-4	0.0	0.0	8.02884451948464	0.0
16	2.8328932905755306E-4	0.0	0.0	8.451937132432096	0.0
17	2.8328932905755306E-4	0.0	0.0	8.994152908248251	0.0
18	2.8328932905755306E-4	0.0	0.0	9.585519382655894	0.0
19	2.8328932905755306E-4	0.0	0.0	9.96342734761867	0.0
20	4.249339935863296E-4	0.0	0.0	10.336519393987468	0.0
21	4.249339935863296E-4	0.0	0.0	10.804230076261488	0.0
22	4.249339935863296E-4	0.0	0.0	11.306785346009587	0.0
23	4.249339935863296E-4	0.0	0.0	11.786535824768553	0.0
24	4.249339935863296E-4	0.0	0.0	12.19093134199821	0.0
25	4.249339935863296E-4	0.0	0.0	12.548867409262428	0.0
26	4.249339935863296E-4	0.0	0.0	12.88088250291788	0.0
27	4.249339935863296E-4	0.0	0.0	13.21091457126993	0.0
28	4.249339935863296E-4	0.0	0.0	13.565592811249985	0.0
29	4.249339935863296E-4	0.0	0.0	13.945058867522578	0.0
30	5.665786581151061E-4	0.0	0.0	14.368718059128149	0.0
31	5.665786581151061E-4	0.0	0.0	14.76801436843477	0.0
32	5.665786581151061E-4	0.0	0.0	15.154137723940215	0.0
33	5.665786581151061E-4	0.0	0.0	15.537994764813199	0.0
34	7.082233226438827E-4	0.0	0.0	15.924684698976758	0.0
35	7.082233226438827E-4	0.0	0.0	16.332762977484165	0.0
36	7.082233226438827E-4	0.0	0.0	16.732909154777957	0.0
37	7.082233226438827E-4	0.0	0.0	17.114074947024896	0.0
38	7.082233226438827E-4	0.0	0.0	17.519037042912668	0.0
39	8.498679871726592E-4	0.0	0.0	17.92371584947138	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAA	20	7.031435E-4	45.0	28
CCCGCGA	20	7.031435E-4	45.0	28
TGTAACG	50	2.1827873E-11	44.999996	1
CGTTTTT	1750	0.0	41.27143	1
CGACCCG	60	3.6379788E-12	41.250004	32
CGGTCTA	55	6.002665E-11	40.909092	31
GTATGCG	50	1.0804797E-9	40.499996	1
GTAAGCG	50	1.0804797E-9	40.499996	1
AACACGT	100	0.0	40.499996	41
GCGTAAG	85	0.0	39.705883	1
GTTCACG	40	3.455698E-7	39.375	1
AAACACG	115	0.0	39.130432	40
TATTGCG	75	0.0	39.0	1
TAGAACG	35	6.2448635E-6	38.57143	1
TCGACGG	35	6.2448635E-6	38.57143	2
TACGGGA	315	0.0	37.857143	4
TATGCGG	155	0.0	37.741936	2
GCGAGAC	120	0.0	37.500004	21
ACACGAC	120	0.0	37.500004	26
CGTAAGG	145	0.0	37.24138	2
>>END_MODULE