FastQCFastQC Report
Sat 18 Jun 2016
SRR3550666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750077
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30040.40049221613247704No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC11420.15225103556034913No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9360.12478718851531242No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT8920.11892112409792595No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC8520.11358833826393823No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7990.10652239703390451No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG253.8895047E-545.010
CGTTTTT17600.040.653411
CGTCATA403.45608E-739.37538
GCGTAAG356.2453873E-638.5714261
ACACGAC950.037.89473726
GACGGGT1200.037.5000044
CGCCCTA301.1395436E-437.50000426
GTTGATC2950.036.6101716
ATAGGGA5800.036.0775834
ACGGGTA751.8189894E-1236.0000045
ATAGACG250.002106642636.01
TCTAGCG250.002106642636.01
TCGTAAG250.002106642636.014
AATCGTG250.002106642636.01
GTACGAT250.002106642636.021
TCACGAC702.1827873E-1135.35714325
TCGATCA702.1827873E-1135.35714317
CGTTAGG702.1827873E-1135.3571432
ACGGTCT702.1827873E-1135.35714330
TTGGACG458.694569E-735.01
Produced by FastQC (version 0.11.5)