##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550666_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	750077
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45493196031874	33.0	31.0	34.0	31.0	34.0
2	32.60161956705778	34.0	31.0	34.0	31.0	34.0
3	32.56015315760915	34.0	31.0	34.0	31.0	34.0
4	36.04052383955247	37.0	35.0	37.0	35.0	37.0
5	36.10425996264384	37.0	35.0	37.0	35.0	37.0
6	36.1520857191995	37.0	36.0	37.0	35.0	37.0
7	36.148863383359306	37.0	37.0	37.0	35.0	37.0
8	36.07698942908528	37.0	37.0	37.0	35.0	37.0
9	37.920025544044144	39.0	38.0	39.0	35.0	39.0
10	37.673736163087256	39.0	37.0	39.0	35.0	39.0
11	37.61155054747712	39.0	37.0	39.0	35.0	39.0
12	37.505184134428866	39.0	37.0	39.0	35.0	39.0
13	37.48523551582038	39.0	37.0	39.0	35.0	39.0
14	38.77979060816423	40.0	38.0	41.0	35.0	41.0
15	38.85574014401188	40.0	38.0	41.0	35.0	41.0
16	38.88991663522545	40.0	38.0	41.0	35.0	41.0
17	38.851287267840505	40.0	38.0	41.0	35.0	41.0
18	38.78754447876685	40.0	38.0	41.0	35.0	41.0
19	38.7142000087991	40.0	38.0	41.0	35.0	41.0
20	38.65564868673483	40.0	38.0	41.0	35.0	41.0
21	38.56495666444911	40.0	38.0	41.0	35.0	41.0
22	38.53610229349787	40.0	38.0	41.0	35.0	41.0
23	38.502924366431714	40.0	37.0	41.0	35.0	41.0
24	38.46308578985891	40.0	37.0	41.0	35.0	41.0
25	38.37264707490031	40.0	37.0	41.0	34.0	41.0
26	38.37616538035429	40.0	37.0	41.0	34.0	41.0
27	38.354679586229146	40.0	37.0	41.0	34.0	41.0
28	38.26930435141992	40.0	37.0	41.0	34.0	41.0
29	38.2233597350672	40.0	37.0	41.0	34.0	41.0
30	38.128116180072176	40.0	37.0	41.0	34.0	41.0
31	38.038640032956614	40.0	37.0	41.0	34.0	41.0
32	37.93213763386959	40.0	36.0	41.0	34.0	41.0
33	37.84722635142792	40.0	36.0	41.0	34.0	41.0
34	37.71542921593383	40.0	36.0	41.0	33.0	41.0
35	37.634398868382846	40.0	36.0	41.0	33.0	41.0
36	37.54826237839582	40.0	36.0	41.0	33.0	41.0
37	37.4585889182044	40.0	36.0	41.0	33.0	41.0
38	37.378454478673525	40.0	35.0	41.0	33.0	41.0
39	37.31560893081644	40.0	35.0	41.0	33.0	41.0
40	37.239770050274835	40.0	35.0	41.0	33.0	41.0
41	37.165800311168056	40.0	35.0	41.0	33.0	41.0
42	37.08601650230576	40.0	35.0	41.0	32.0	41.0
43	37.00976166446912	39.0	35.0	41.0	32.0	41.0
44	36.926820846393106	39.0	35.0	41.0	32.0	41.0
45	36.83856057444769	39.0	35.0	41.0	32.0	41.0
46	36.76074722995106	39.0	35.0	41.0	31.0	41.0
47	36.66894065542604	39.0	35.0	41.0	31.0	41.0
48	36.590874003602295	39.0	35.0	41.0	31.0	41.0
49	36.51139816312192	39.0	35.0	41.0	31.0	41.0
50	36.398187119455734	39.0	35.0	41.0	31.0	41.0
51	35.46629879332389	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	16.0
10	21.0
11	19.0
12	24.0
13	18.0
14	17.0
15	23.0
16	38.0
17	71.0
18	149.0
19	248.0
20	465.0
21	699.0
22	1109.0
23	1736.0
24	2562.0
25	3836.0
26	5143.0
27	5901.0
28	6688.0
29	7529.0
30	8764.0
31	10827.0
32	13833.0
33	19077.0
34	39110.0
35	56286.0
36	46924.0
37	72057.0
38	134311.0
39	312103.0
40	460.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.673218882861356	21.92201600635668	23.555981585890514	11.848783524891445
2	34.10583180126841	24.484419599587774	24.038065425283005	17.371683173860816
3	32.1558986610708	23.940475444521027	26.60326873107694	17.30035716333123
4	29.431111739194776	25.85254580529732	26.013729257129604	18.7026131983783
5	25.58284016174339	30.472071533989176	24.146720936650503	19.798367367616926
6	26.357293984484258	35.05413444219727	23.65863771319478	14.929933860123695
7	79.57089738786817	6.691579664487779	8.819494531894726	4.918028415749316
8	80.53559834523656	6.138569773503254	8.66591029987588	4.659921581384311
9	73.33953714085354	9.189723188419324	10.908080103776012	6.562659566951126
10	40.172009007075275	26.393423608509526	18.36438125685763	15.070186127557573
11	30.706847430330487	25.879076414821412	23.97980474004669	19.43427141480141
12	28.34562318268658	23.24894644149867	28.03965459546153	20.365775780353218
13	25.35406364946532	24.269108371540522	29.312057295450998	21.06477068354316
14	21.724969569790833	27.05242261794456	29.477240336658774	21.745367475605835
15	21.81882660046902	26.047325807883727	32.25695495262486	19.876892639022394
16	24.02766649290673	25.528312426590873	29.869066775811014	20.574954304691385
17	23.344803266864602	25.81028347756297	28.16790809476894	22.67700516080349
18	23.934209421166095	26.468082610185355	28.66052418618355	20.937183782465
19	25.0822248915778	27.2966642091412	26.45235089197509	21.168760007305917
20	26.685660272212054	27.130681250058323	26.561939640863535	19.62171883686608
21	25.279537967435346	27.482245156163966	27.34159292979254	19.89662394660815
22	24.429625225143553	25.41432412938938	27.127614898203785	23.02843574726328
23	24.143387945504262	26.341562266273993	27.105750476284435	22.409299311937307
24	23.68410176555207	25.411524416826538	28.418948987903907	22.48542482971748
25	23.374533547889083	26.771651443785103	26.798848651538442	23.05496635678737
26	22.649674633404302	27.9883265318094	26.853509706336816	22.50848912844948
27	23.58424535081065	27.45424803053553	27.35065866571032	21.610847952943498
28	22.484358272550683	27.65662725293537	27.580368415509344	22.278646059004608
29	24.20911453090816	26.27170277184876	26.728322558883953	22.79086013835913
30	25.052227971261615	26.284368138204478	26.405955655219397	22.25744823531451
31	24.697864352593133	28.06151901738088	25.049161619407073	22.19145501061891
32	25.119021113832314	28.625727758616783	24.62120555622956	21.634045571321344
33	24.526415288030428	28.08791630725912	24.783988843812036	22.601679560898415
34	23.00270505561429	28.152176376558675	27.26053458511593	21.58458398271111
35	24.22617944557692	27.652627663559876	26.08332211226314	22.037870778600062
36	24.367231630885897	29.29445910219884	24.476820379774345	21.86148888714092
37	23.712765489409755	29.583762733692677	26.24783855524166	20.455633221655912
38	22.76032993945955	29.839336494786533	24.993567327087753	22.406766238666165
39	24.341900898174455	26.199843482735773	26.65499675366662	22.80325886542315
40	23.891547134494193	25.604037985433497	28.566133876921967	21.938281003150344
41	22.020139265702056	27.087752324094726	27.477045689975828	23.41506272022739
42	23.451192344252654	27.34452596200124	26.540608497527586	22.66367319621852
43	23.92794339781116	25.787085859185122	27.411852383155328	22.873118359848387
44	23.003638293135236	26.29503370987245	26.335696201856614	24.3656317951357
45	22.78592731146269	25.73789090986659	26.58593717711648	24.89024460155424
46	23.08496327710355	26.74298771992742	26.550740790612164	23.621308212356865
47	22.896982576455486	26.26996961645271	28.052053322525556	22.78099448456625
48	22.40050021531123	25.899341000990567	28.077650694528693	23.62250808916951
49	22.39556738841479	25.753355988785152	28.156042646288316	23.695033976511745
50	21.532855960121427	25.479650755855733	28.54000322633543	24.44749005768741
51	21.346341775577706	25.490716286461257	27.259201388657434	25.903740549303606
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	375.0
1	858.0
2	1341.0
3	3324.5
4	5308.0
5	3610.5
6	1913.0
7	1948.0
8	1983.0
9	2001.0
10	2019.0
11	1986.0
12	1953.0
13	2011.0
14	2069.0
15	2059.0
16	2049.0
17	1928.5
18	1808.0
19	1890.0
20	1972.0
21	2100.0
22	2228.0
23	2486.0
24	2744.0
25	3170.0
26	4918.5
27	6241.0
28	7618.5
29	8996.0
30	10768.0
31	12540.0
32	13679.5
33	14819.0
34	17030.5
35	19242.0
36	20735.0
37	22228.0
38	23856.0
39	25484.0
40	30059.0
41	34634.0
42	36879.5
43	39125.0
44	43520.5
45	47916.0
46	53159.5
47	58403.0
48	63877.5
49	69352.0
50	68012.0
51	66672.0
52	59651.0
53	52630.0
54	47378.0
55	42126.0
56	39752.5
57	37379.0
58	36254.0
59	35129.0
60	34349.0
61	33569.0
62	31313.5
63	29058.0
64	24518.5
65	19979.0
66	16485.0
67	12991.0
68	11310.5
69	9630.0
70	8605.5
71	7581.0
72	6420.0
73	5259.0
74	4435.0
75	2772.0
76	1933.0
77	1497.5
78	1062.0
79	876.0
80	690.0
81	467.5
82	245.0
83	164.5
84	84.0
85	78.5
86	73.0
87	49.0
88	25.0
89	14.5
90	4.0
91	4.0
92	4.0
93	3.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	750077.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.385018252042222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.71681584388789	21.073999425509964
2	9.401425231074176	5.525221040206498
3	3.39256729191005	2.990719553821748
4	1.8148946172217344	2.133228458103754
5	1.249847230889371	1.8363391845974297
6	0.972474687653372	1.7145711867806037
7	0.8496826563899218	1.7477558256523098
8	0.722412204673454	1.6982476655862007
9	0.6590547040594136	1.7429701057092135
>10	9.086666999155458	54.1681363123824
>50	0.09004538831778257	1.7416731032960917
>100	0.03911062319584731	2.2138077665599
>500	0.004092972194914253	0.8592770698406469
>1k	9.095493766476118E-4	0.5540533019532383
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3004	0.40049221613247704	No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	1142	0.15225103556034913	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	936	0.12478718851531242	No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	892	0.11892112409792595	No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	852	0.11358833826393823	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	799	0.10652239703390451	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.999589375490783E-4	0.0	0.0	0.12252075453586765	0.0
2	3.999589375490783E-4	0.0	0.0	0.49448256645651045	0.0
3	5.33278583398771E-4	0.0	0.0	0.7805865264499512	0.0
4	5.33278583398771E-4	0.0	0.0	1.155614690225137	0.0
5	5.33278583398771E-4	0.0	0.0	2.147112896409302	0.0
6	5.33278583398771E-4	0.0	0.0	2.9612959736133755	0.0
7	5.33278583398771E-4	0.0	0.0	3.6265610064033424	0.0
8	5.33278583398771E-4	0.0	0.0	4.661254777842808	0.0
9	5.33278583398771E-4	0.0	0.0	5.130273291942027	0.0
10	5.33278583398771E-4	0.0	0.0	5.960588046293914	0.0
11	5.33278583398771E-4	0.0	0.0	7.023012304070115	0.0
12	5.33278583398771E-4	0.0	0.0	7.9055883595950815	0.0
13	5.33278583398771E-4	0.0	0.0	8.269817632056442	0.0
14	5.33278583398771E-4	0.0	0.0	8.441533335910847	0.0
15	5.33278583398771E-4	0.0	0.0	8.701639964963597	0.0
16	5.33278583398771E-4	0.0	0.0	9.201321997608245	0.0
17	5.33278583398771E-4	0.0	0.0	9.753665290363523	0.0
18	5.33278583398771E-4	0.0	0.0	10.356270089604134	0.0
19	5.33278583398771E-4	0.0	0.0	10.737964235671804	0.0
20	5.33278583398771E-4	0.0	0.0	11.127390921198757	0.0
21	5.33278583398771E-4	0.0	0.0	11.609608080237095	0.0
22	5.33278583398771E-4	0.0	0.0	12.11488953800743	0.0
23	5.33278583398771E-4	0.0	0.0	12.615371488527177	0.0
24	5.33278583398771E-4	0.0	0.0	13.034395135432762	0.0
25	5.33278583398771E-4	0.0	0.0	13.422488624501218	0.0
26	5.33278583398771E-4	0.0	0.0	13.788584372004474	0.0
27	5.33278583398771E-4	0.0	0.0	14.141214835276912	0.0
28	5.33278583398771E-4	0.0	0.0	14.508510459592815	0.0
29	5.33278583398771E-4	0.0	0.0	14.878472476825712	0.0
30	5.33278583398771E-4	0.0	0.0	15.310428129378717	0.0
31	6.665982292484639E-4	0.0	0.0	15.719586122491425	0.0
32	6.665982292484639E-4	0.0	0.0	16.11741194570691	0.0
33	7.999178750981566E-4	0.0	0.0	16.52417018519432	0.0
34	7.999178750981566E-4	0.0	0.0	16.903464577636697	0.0
35	7.999178750981566E-4	0.0	0.0	17.305423309873518	0.0
36	7.999178750981566E-4	0.0	0.0	17.714714622632076	0.0
37	7.999178750981566E-4	0.0	0.0	18.123739296098933	0.0
38	7.999178750981566E-4	0.0	0.0	18.54089646796262	0.0
39	7.999178750981566E-4	0.0	0.0	18.978584865287164	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTCG	25	3.8895047E-5	45.0	10
CGTTTTT	1760	0.0	40.65341	1
CGTCATA	40	3.45608E-7	39.375	38
GCGTAAG	35	6.2453873E-6	38.571426	1
ACACGAC	95	0.0	37.894737	26
GACGGGT	120	0.0	37.500004	4
CGCCCTA	30	1.1395436E-4	37.500004	26
GTTGATC	295	0.0	36.61017	16
ATAGGGA	580	0.0	36.077583	4
ACGGGTA	75	1.8189894E-12	36.000004	5
ATAGACG	25	0.0021066426	36.0	1
TCTAGCG	25	0.0021066426	36.0	1
TCGTAAG	25	0.0021066426	36.0	14
AATCGTG	25	0.0021066426	36.0	1
GTACGAT	25	0.0021066426	36.0	21
TCACGAC	70	2.1827873E-11	35.357143	25
TCGATCA	70	2.1827873E-11	35.357143	17
CGTTAGG	70	2.1827873E-11	35.357143	2
ACGGTCT	70	2.1827873E-11	35.357143	30
TTGGACG	45	8.694569E-7	35.0	1
>>END_MODULE