FastQCFastQC Report
Sat 18 Jun 2016
SRR3550665_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550665_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554839
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31540.5684531909256559No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC11410.2056452412321412No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT8600.15499991889539128No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG8150.14688945802295802No Hit
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC7380.13301155830790554No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTT6730.12129644815883527No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC6100.10994180293742868No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG253.8881877E-545.01
CGTGCAA207.030128E-445.010
GCGTTAG900.045.01
TTACGCG207.030128E-445.01
TATACGG207.030128E-445.02
CGATACT207.030128E-445.010
TGCGTTA207.030128E-445.034
AATCGGC207.030128E-445.010
ACGGTAA207.030128E-445.014
CGGACGT207.030128E-445.06
ATAGCGG302.1634642E-644.9999962
CGTTTTT18400.043.0434761
TAGTATG700.041.7857131
GTTGTAG603.6379788E-1241.2499961
CGTTAGG1150.041.0869562
GTTAGCG451.9252184E-840.01
CGTAAGG900.040.02
TGTCGCA451.9252184E-840.039
TAACGCC403.4539153E-739.37512
ACGTAAG403.4539153E-739.3751