##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550664_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	453036
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.274638218596316	33.0	31.0	34.0	30.0	34.0
2	32.406652009994794	34.0	31.0	34.0	30.0	34.0
3	32.38522104203639	34.0	31.0	34.0	30.0	34.0
4	35.89998808041745	37.0	35.0	37.0	35.0	37.0
5	35.994731103047	37.0	35.0	37.0	35.0	37.0
6	36.052627164287166	37.0	35.0	37.0	35.0	37.0
7	36.08776344484765	37.0	36.0	37.0	35.0	37.0
8	36.00388490097917	37.0	35.0	37.0	35.0	37.0
9	37.854075614299965	39.0	38.0	39.0	35.0	39.0
10	37.61082783708138	39.0	37.0	39.0	35.0	39.0
11	37.55192302598469	39.0	37.0	39.0	35.0	39.0
12	37.4566392074802	39.0	37.0	39.0	35.0	39.0
13	37.38858501311154	39.0	37.0	39.0	35.0	39.0
14	38.62380031608967	40.0	38.0	41.0	35.0	41.0
15	38.718867816244185	40.0	38.0	41.0	35.0	41.0
16	38.759864558224955	40.0	38.0	41.0	35.0	41.0
17	38.732436274379964	40.0	38.0	41.0	35.0	41.0
18	38.67912704509134	40.0	38.0	41.0	35.0	41.0
19	38.64703467274124	40.0	38.0	41.0	35.0	41.0
20	38.58458488950105	40.0	38.0	41.0	35.0	41.0
21	38.505220335690765	40.0	38.0	41.0	34.0	41.0
22	38.477895796360556	40.0	38.0	41.0	34.0	41.0
23	38.453787336988675	40.0	37.0	41.0	34.0	41.0
24	38.42972302421882	40.0	37.0	41.0	34.0	41.0
25	38.32981043449086	40.0	37.0	41.0	34.0	41.0
26	38.35691424081089	40.0	37.0	41.0	34.0	41.0
27	38.33947854033675	40.0	37.0	41.0	34.0	41.0
28	38.294742581163526	40.0	37.0	41.0	34.0	41.0
29	38.27078422023857	40.0	37.0	41.0	34.0	41.0
30	38.191148164825755	40.0	37.0	41.0	34.0	41.0
31	38.12669191852303	40.0	37.0	41.0	34.0	41.0
32	38.04365216009324	40.0	37.0	41.0	34.0	41.0
33	37.973463477516134	40.0	37.0	41.0	34.0	41.0
34	37.88468686815176	40.0	37.0	41.0	34.0	41.0
35	37.81795486451408	40.0	37.0	41.0	33.0	41.0
36	37.720212080276184	40.0	37.0	41.0	33.0	41.0
37	37.67765034125323	40.0	36.0	41.0	33.0	41.0
38	37.63223893906886	40.0	36.0	41.0	33.0	41.0
39	37.54330340193715	40.0	36.0	41.0	33.0	41.0
40	37.46029013146858	40.0	36.0	41.0	33.0	41.0
41	37.390132351512904	40.0	36.0	41.0	33.0	41.0
42	37.33673262169011	40.0	35.0	41.0	33.0	41.0
43	37.27263396286388	40.0	35.0	41.0	33.0	41.0
44	37.20973829894313	40.0	35.0	41.0	33.0	41.0
45	37.11382759868973	39.0	35.0	41.0	32.0	41.0
46	37.028567266177525	39.0	35.0	41.0	32.0	41.0
47	36.9414549837099	39.0	35.0	41.0	32.0	41.0
48	36.89697286749839	39.0	35.0	41.0	32.0	41.0
49	36.84077865776671	39.0	35.0	41.0	32.0	41.0
50	36.73721514405036	39.0	35.0	41.0	31.0	41.0
51	35.750911627332044	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	8.0
10	12.0
11	7.0
12	8.0
13	9.0
14	6.0
15	11.0
16	20.0
17	33.0
18	68.0
19	136.0
20	233.0
21	344.0
22	561.0
23	855.0
24	1273.0
25	2037.0
26	2680.0
27	3240.0
28	3635.0
29	4285.0
30	5336.0
31	6639.0
32	8615.0
33	11814.0
34	23086.0
35	34207.0
36	28482.0
37	44263.0
38	84155.0
39	186746.0
40	228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1324133181469	22.38034063518131	25.422262248474738	10.064983798197053
2	32.09457968020201	25.180338869317232	25.350303287155988	17.37477816332477
3	31.60455239760196	24.85939307251521	27.135371140483315	16.400683389399518
4	28.626201891240434	26.638942600588035	25.59619986049674	19.1386556476748
5	25.295561500631297	30.216803962599	25.262893015124625	19.22474152164508
6	26.744894445474532	34.95925268632074	23.774269594469313	14.52158327373542
7	79.56806964567937	6.604773130612136	8.709462382680405	5.117694841028086
8	78.96613072691795	6.288241994013721	8.974783460917013	5.770843818151317
9	72.4361860867569	9.385788325872557	10.989855110852117	7.1881704765184224
10	37.90427250814505	31.910929815732082	16.83861768159705	13.346179994525823
11	30.245057787902063	25.34368129685058	25.17746934018489	19.23379157506247
12	28.44233129376032	22.846528752681905	29.11578770782013	19.595352245737647
13	25.489585816579698	24.792510970430605	30.10732038954962	19.61058282344008
14	21.264976734740728	29.633627349702895	28.29466090994976	20.80673500560662
15	20.206120484906275	27.20004591246612	33.37196161011487	19.22187199251274
16	22.35981246523455	26.94355415463672	30.186563540204308	20.510069839924423
17	22.60372244148368	26.77226533873688	27.02610830044411	23.597903919335327
18	22.66089229112035	27.584562816200037	28.99990287747552	20.754642015204087
19	24.438675955111737	28.097104865838475	26.395915556379624	21.06830362267016
20	25.7048005015054	28.193565191287227	26.78219832419499	19.319435983012387
21	24.24200284304117	27.62230816094085	27.98563469569747	20.150054300320505
22	22.71342674754324	26.65726344043299	26.351768954343584	24.277540857680187
23	22.959323320883993	27.084823281152048	27.479052437333902	22.47680096063006
24	23.923926575371492	25.889333298016055	27.776600535056815	22.410139591555637
25	22.223840930963544	28.239036191384347	25.50238833117015	24.034734546481957
26	21.7068842211215	29.042062882419938	26.467433051678015	22.78361984478055
27	22.92422677226534	28.386265109174545	26.610909508295144	22.07859861026497
28	20.406766791160084	27.492737883965074	28.690435197202873	23.410060127671972
29	23.562145171686137	24.6810408002896	27.860479078925295	23.896334949098968
30	23.38467583150125	26.291508842564387	26.110287041206437	24.213528284727925
31	23.452882331646933	27.185477533794224	26.270759939607448	23.090880194951392
32	25.046574665148025	26.953928606115184	26.03987321095895	21.95962351777784
33	23.04430552980337	26.21094129384861	26.341173769854937	24.40357940649308
34	22.132898930769297	25.022514767038377	29.977529379563656	22.867056922628663
35	23.686638589427773	24.238471114878287	27.752982102967533	24.321908192726404
36	23.229500525344566	25.840551302766222	27.771082209802312	23.158865962086896
37	21.94240634298378	26.34161523587529	30.827792934777808	20.888185486363113
38	21.615721487917074	26.24846590557925	28.275015672043725	23.860796934459955
39	22.767506335037393	24.340008299561184	30.016157656345193	22.876327709056234
40	23.364368394564668	24.30336661987127	30.42341006012767	21.908854925436387
41	21.864487590390166	25.18100106834777	28.8272896635146	24.127221677747464
42	23.604305176630554	24.526969159183817	29.263899557651047	22.60482610653458
43	23.114057161020316	24.571115761219858	29.06391545042778	23.250911627332044
44	22.01613116838397	24.985431621328104	27.646368059050495	25.352069151237426
45	21.94284780900414	24.300276357728745	26.986817824632038	26.77005800863508
46	23.241640840904477	25.34897888909491	27.364492005050373	24.044888264950245
47	21.552150380985175	24.132298536981608	30.492278759303897	23.82327232272932
48	21.40779099232732	23.283800845848894	30.009756399049962	25.298651762773822
49	22.17395527066282	23.02201149577517	31.135494750969016	23.668538482592997
50	20.715351539392014	23.182263661166	30.37661466196947	25.72577013747252
51	20.633459592615154	23.21382848162177	27.818760540001236	28.333951385761836
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	158.0
1	360.5
2	563.0
3	1248.0
4	1933.0
5	1359.0
6	785.0
7	797.5
8	810.0
9	839.0
10	868.0
11	910.0
12	952.0
13	952.0
14	952.0
15	977.0
16	1002.0
17	983.5
18	965.0
19	1036.5
20	1108.0
21	1175.5
22	1243.0
23	1334.0
24	1425.0
25	1789.5
26	2629.0
27	3104.0
28	3744.5
29	4385.0
30	5249.5
31	6114.0
32	6938.5
33	7763.0
34	9199.5
35	10636.0
36	11736.0
37	12836.0
38	14394.0
39	15952.0
40	17779.5
41	19607.0
42	22174.0
43	24741.0
44	28703.5
45	32666.0
46	38817.5
47	44969.0
48	49412.5
49	53856.0
50	51137.0
51	48418.0
52	42112.0
53	35806.0
54	30845.5
55	25885.0
56	23163.0
57	20441.0
58	19290.0
59	18139.0
60	17227.0
61	16315.0
62	14831.0
63	13347.0
64	11008.0
65	8669.0
66	6863.0
67	5057.0
68	4286.0
69	3515.0
70	3142.0
71	2769.0
72	2054.0
73	1339.0
74	1115.5
75	707.5
76	523.0
77	381.0
78	239.0
79	164.0
80	89.0
81	52.0
82	15.0
83	12.0
84	9.0
85	11.5
86	14.0
87	9.0
88	4.0
89	3.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	453036.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.32801067560311
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.79720123908841	27.54702715682323
2	10.200514300480053	7.615298134099232
3	3.643193090392182	4.079794517143285
4	1.9852046518984696	2.9641496175729207
5	1.2778736852336139	2.385024128223631
6	0.9940412421813196	2.2263349260080445
7	0.8086201649023356	2.1128927503587627
8	0.6926190882275546	2.0683274175587716
9	0.5918856052388305	1.9884520971981785
>10	5.890256918053508	41.12780529707482
>50	0.07471170901132632	1.9849454270647555
>100	0.04032060486325547	2.6310265713111898
>500	0.0023718002860738515	0.6449744619164471
>1k	0.0011859001430369258	0.6239474976466933
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	1767	0.39003522898842474	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1052	0.23221112670957716	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	893	0.19711457809092436	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	849	0.18740232564299525	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	648	0.14303499059677377	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC	524	0.11566409733442817	No Hit
ACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	475	0.10484817983559805	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4146602036041285E-4	0.0	0.0	0.19358284992804103	0.0
2	4.4146602036041285E-4	0.0	0.0	1.056648919732648	0.0
3	4.4146602036041285E-4	0.0	0.0	1.5532981926381126	0.0
4	4.4146602036041285E-4	0.0	0.0	2.2499315727668443	0.0
5	4.4146602036041285E-4	0.0	0.0	4.190174732250859	0.0
6	4.4146602036041285E-4	0.0	0.0	5.249693181115849	0.0
7	4.4146602036041285E-4	0.0	0.0	6.299499377532912	0.0
8	4.4146602036041285E-4	0.0	0.0	7.973759259749777	0.0
9	4.4146602036041285E-4	0.0	0.0	8.646332741768866	0.0
10	4.4146602036041285E-4	0.0	0.0	10.012670074784344	0.0
11	4.4146602036041285E-4	0.0	0.0	11.86903469039988	0.0
12	4.4146602036041285E-4	0.0	0.0	13.360748373197715	0.0
13	6.621990305406193E-4	0.0	0.0	13.971075146345985	0.0
14	6.621990305406193E-4	0.0	0.0	14.218958316778357	0.0
15	6.621990305406193E-4	0.0	0.0	14.641882764283633	0.0
16	8.829320407208257E-4	0.0	0.0	15.455504639807874	0.0
17	8.829320407208257E-4	0.0	0.0	16.452776379802046	0.0
18	8.829320407208257E-4	0.0	0.0	17.508763100504154	0.0
19	8.829320407208257E-4	0.0	0.0	18.213342868999373	0.0
20	0.001103665050901032	0.0	0.0	18.91461164234189	0.0
21	0.001103665050901032	0.0	0.0	19.768848391739287	0.0
22	0.001103665050901032	0.0	0.0	20.674736665518854	0.0
23	0.001103665050901032	0.0	0.0	21.530739278997697	0.0
24	0.0013243980610812387	0.0	0.0	22.233553183411473	0.0
25	0.0013243980610812387	0.0	0.0	22.835050636152534	0.0
26	0.0013243980610812387	0.0	0.0	23.398802744152782	0.0
27	0.0013243980610812387	0.0	0.0	23.95151820164402	0.0
28	0.001545131071261445	0.0	0.0	24.49893606689093	0.0
29	0.001545131071261445	0.0	0.0	25.123389752690734	0.0
30	0.001545131071261445	0.0	0.0	25.786913181292437	0.0
31	0.001545131071261445	0.0	0.0	26.42483158071323	0.0
32	0.001545131071261445	0.0	0.0	27.01043625672132	0.0
33	0.001545131071261445	0.0	0.0	27.572863966660485	0.0
34	0.001545131071261445	0.0	0.0	28.149639322261365	0.0
35	0.001545131071261445	0.0	0.0	28.723103682709542	0.0
36	0.001545131071261445	0.0	0.0	29.33100239274583	0.0
37	0.001545131071261445	0.0	0.0	29.923229059059324	0.0
38	0.001545131071261445	0.0	0.0	30.522519181698584	0.0
39	0.0017658640814416514	0.0	0.0	31.134170352907937	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAG	25	3.887051E-5	45.0	1
CGGGTAT	40	6.7993824E-9	45.0	6
TCGTTTG	20	7.028757E-4	45.0	1
GTACCGT	20	7.028757E-4	45.0	13
ATTAGCG	20	7.028757E-4	45.0	1
TTGTGCG	40	6.7993824E-9	45.0	1
CTATACT	20	7.028757E-4	45.0	31
CGATTCG	20	7.028757E-4	45.0	10
TTGACGG	85	0.0	45.0	2
CGAATAT	80	0.0	45.0	14
CGGTCTA	20	7.028757E-4	45.0	31
TACCGTT	20	7.028757E-4	45.0	14
CGTATGG	30	2.1625801E-6	44.999996	2
CCAACGG	30	2.1625801E-6	44.999996	2
GCTACGA	85	0.0	42.35294	10
TTAGCGG	75	0.0	42.0	2
TAGCGCG	45	1.9239451E-8	40.0	1
CTACGAA	90	0.0	40.0	11
CTTGCGT	45	1.9239451E-8	40.0	15
TGCGTCT	45	1.9239451E-8	40.0	17
>>END_MODULE