Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550660_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 664470 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 5972 | 0.8987614188751938 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTC | 4767 | 0.7174138787304167 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCG | 2634 | 0.3964061582915708 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGC | 2618 | 0.3939982241485695 | No Hit |
GCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC | 1823 | 0.27435399641819797 | No Hit |
GAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT | 1282 | 0.19293572320797028 | No Hit |
CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT | 985 | 0.1482384456785107 | TruSeq Adapter, Index 13 (95% over 23bp) |
CCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC | 873 | 0.13138290667750238 | No Hit |
CGTTTCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTT | 861 | 0.1295769560702515 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACGCTAG | 20 | 7.031136E-4 | 45.0 | 1 |
TATAGCG | 20 | 7.031136E-4 | 45.0 | 1 |
ACGTAAG | 50 | 2.1827873E-11 | 45.0 | 1 |
TCGACAC | 25 | 3.8890226E-5 | 45.0 | 34 |
ACGGGTC | 30 | 2.1641135E-6 | 44.999996 | 5 |
GCGATAC | 30 | 2.1641135E-6 | 44.999996 | 9 |
CGTTTTT | 3255 | 0.0 | 42.09677 | 1 |
TAGCGAG | 60 | 3.6379788E-12 | 41.249996 | 1 |
GCGATTC | 45 | 1.9261279E-8 | 40.0 | 9 |
GCGATCT | 45 | 1.9261279E-8 | 40.0 | 9 |
TTAACGG | 45 | 1.9261279E-8 | 40.0 | 2 |
ATTGCGG | 90 | 0.0 | 40.0 | 2 |
TCGATCA | 40 | 3.4552795E-7 | 39.375 | 17 |
TACGGCT | 350 | 0.0 | 39.214287 | 7 |
CGTAAGG | 75 | 0.0 | 39.000004 | 2 |
GCTACGA | 65 | 9.094947E-12 | 38.076927 | 10 |
AAGGGCG | 215 | 0.0 | 37.67442 | 5 |
TTTCGCG | 30 | 1.13940354E-4 | 37.499996 | 1 |
TGAACCG | 30 | 1.13940354E-4 | 37.499996 | 1 |
AATGACG | 30 | 1.13940354E-4 | 37.499996 | 1 |