FastQCFastQC Report
Sat 18 Jun 2016
SRR3550660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664470
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59720.8987614188751938No Hit
GAATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTC47670.7174138787304167No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCG26340.3964061582915708No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGC26180.3939982241485695No Hit
GCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC18230.27435399641819797No Hit
GAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT12820.19293572320797028No Hit
CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT9850.1482384456785107TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC8730.13138290667750238No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTT8610.1295769560702515No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAG207.031136E-445.01
TATAGCG207.031136E-445.01
ACGTAAG502.1827873E-1145.01
TCGACAC253.8890226E-545.034
ACGGGTC302.1641135E-644.9999965
GCGATAC302.1641135E-644.9999969
CGTTTTT32550.042.096771
TAGCGAG603.6379788E-1241.2499961
GCGATTC451.9261279E-840.09
GCGATCT451.9261279E-840.09
TTAACGG451.9261279E-840.02
ATTGCGG900.040.02
TCGATCA403.4552795E-739.37517
TACGGCT3500.039.2142877
CGTAAGG750.039.0000042
GCTACGA659.094947E-1238.07692710
AAGGGCG2150.037.674425
TTTCGCG301.13940354E-437.4999961
TGAACCG301.13940354E-437.4999961
AATGACG301.13940354E-437.4999961