##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550660_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	664470
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36554697729017	33.0	31.0	34.0	30.0	34.0
2	32.517656177103554	34.0	31.0	34.0	31.0	34.0
3	32.456633106084546	34.0	31.0	34.0	30.0	34.0
4	35.976992189263626	37.0	35.0	37.0	35.0	37.0
5	36.049239243306694	37.0	35.0	37.0	35.0	37.0
6	36.094779297786204	37.0	35.0	37.0	35.0	37.0
7	36.152887263533344	37.0	37.0	37.0	35.0	37.0
8	36.051776603909886	37.0	37.0	37.0	35.0	37.0
9	37.91955543515885	39.0	38.0	39.0	35.0	39.0
10	37.677254052101674	39.0	37.0	39.0	35.0	39.0
11	37.58608966544765	39.0	37.0	39.0	35.0	39.0
12	37.58023838548016	39.0	37.0	39.0	35.0	39.0
13	37.57159992174214	39.0	37.0	39.0	35.0	39.0
14	38.91560642316433	40.0	38.0	41.0	35.0	41.0
15	38.96889701566662	40.0	38.0	41.0	35.0	41.0
16	39.02708323927341	40.0	38.0	41.0	35.0	41.0
17	38.96709557993589	40.0	38.0	41.0	35.0	41.0
18	38.850127169021924	40.0	38.0	41.0	35.0	41.0
19	38.76881574788929	40.0	38.0	41.0	35.0	41.0
20	38.628190888979184	40.0	38.0	41.0	35.0	41.0
21	38.588699264075125	40.0	38.0	41.0	35.0	41.0
22	38.54655740665493	40.0	38.0	41.0	35.0	41.0
23	38.49997140578205	40.0	38.0	41.0	35.0	41.0
24	38.47996749288907	40.0	37.0	41.0	35.0	41.0
25	38.42354809095971	40.0	37.0	41.0	35.0	41.0
26	38.419857931885566	40.0	37.0	41.0	35.0	41.0
27	38.3960404532936	40.0	37.0	41.0	35.0	41.0
28	38.32384155793339	40.0	37.0	41.0	34.0	41.0
29	38.286362062997576	40.0	37.0	41.0	34.0	41.0
30	38.20226496305326	40.0	37.0	41.0	34.0	41.0
31	38.08346050235526	40.0	37.0	41.0	34.0	41.0
32	37.9045329360242	40.0	37.0	41.0	34.0	41.0
33	37.72923984529023	40.0	37.0	41.0	33.0	41.0
34	37.57082486793986	40.0	37.0	41.0	33.0	41.0
35	37.47136815808088	40.0	37.0	41.0	33.0	41.0
36	37.354295905007	40.0	37.0	41.0	33.0	41.0
37	37.293479013348986	40.0	36.0	41.0	33.0	41.0
38	37.21667644889912	40.0	36.0	41.0	32.0	41.0
39	37.129668758559454	40.0	36.0	41.0	32.0	41.0
40	37.03382695983265	40.0	36.0	41.0	31.0	41.0
41	36.98182912697338	40.0	36.0	41.0	31.0	41.0
42	36.94561379746264	40.0	36.0	41.0	31.0	41.0
43	36.887960329284994	40.0	35.0	41.0	31.0	41.0
44	36.82838803858714	40.0	35.0	41.0	31.0	41.0
45	36.7418092615167	40.0	35.0	41.0	31.0	41.0
46	36.656109380408445	39.0	35.0	41.0	31.0	41.0
47	36.56161000496636	39.0	35.0	41.0	31.0	41.0
48	36.5401161828224	39.0	35.0	41.0	31.0	41.0
49	36.49093713786928	39.0	35.0	41.0	31.0	41.0
50	36.38977831956296	39.0	35.0	41.0	30.0	41.0
51	35.499361897452104	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	4.0
10	8.0
11	12.0
12	7.0
13	14.0
14	12.0
15	14.0
16	26.0
17	56.0
18	116.0
19	221.0
20	419.0
21	801.0
22	1244.0
23	1857.0
24	2687.0
25	4100.0
26	6013.0
27	7549.0
28	7810.0
29	7835.0
30	8312.0
31	9755.0
32	12028.0
33	15919.0
34	28152.0
35	41556.0
36	39843.0
37	59559.0
38	115950.0
39	292173.0
40	411.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.854921967884174	20.959561755985973	25.11279666501121	14.072719611118636
2	32.906376510602435	25.166824687344803	25.306334371754936	16.620464430297833
3	29.82075940223035	25.684229536322185	28.980992369858683	15.514018691588785
4	26.836877511400065	24.55505891913856	31.082667389046907	17.525396180414464
5	25.857901786386144	28.74561680738032	26.897075864975093	18.499405541258447
6	25.60386473429952	34.137282345327854	25.57888241756588	14.67997050280675
7	78.14257980044246	5.114301623850587	12.305145454271825	4.437973121435128
8	79.29763570966334	5.945941878489623	10.522521709031258	4.233900702815778
9	72.78477583638087	7.77762728189384	12.091591794964408	7.3460050867608775
10	35.43440636898581	34.841452586271764	18.05122879889235	11.672912245850075
11	25.42763405420862	23.5711168299547	32.70787243968877	18.293376676147908
12	24.53293602419974	21.105994251057233	34.10612969735278	20.25494002739025
13	22.72743690460066	22.109049317501167	36.03684139238792	19.126672385510258
14	18.47442322452481	27.53066353635228	33.067106114647764	20.927807124475144
15	17.764082652339457	26.19441058287056	37.00091802489202	19.040588739897967
16	20.77129140518007	25.778138967899228	33.310758950742695	20.13981067617801
17	20.332896895269915	26.134663716947344	31.70000150495884	21.832437882823903
18	20.122955137176998	26.905955122127413	31.821903170948275	21.149186569747318
19	21.625205050641867	27.46173642150887	30.399416075970322	20.51364245187894
20	24.227278883922523	26.495703342513583	31.017352175418	18.25966559814589
21	23.109696449802097	26.889400574894278	31.118184417656174	18.88271855764745
22	21.46598040543591	24.809998946528815	30.774150826974882	22.949869821060396
23	21.02487696961488	26.68668261923036	31.412403870754137	20.87603654040062
24	21.37658585037699	25.37330504010715	31.373876924466117	21.876232185049737
25	20.548106009300646	28.275467665959336	29.536924165124084	21.639502159615933
26	20.376540701611813	27.685523800924045	30.165996959983143	21.771938537481
27	22.74730236128042	26.794287176245728	29.8285851881951	20.629825274278748
28	19.897813294806387	27.62111156259876	30.996282751666744	21.48479239092811
29	21.965777235992594	24.647914879528045	30.34478607010098	23.041521814378378
30	22.79395608530107	25.903502039219227	29.565819374840096	21.73672250063961
31	22.376029015606424	26.749890890484146	27.91894291691122	22.955137176998207
32	23.835989585684832	27.312745496410674	27.723448763676313	21.12781615422818
33	23.09524884494409	28.067030866705796	27.32267822475055	21.51504206359956
34	21.133835989585684	27.174289283188102	27.846253404969374	23.845621322256836
35	22.24073321594654	25.484822490104897	29.093262299276113	23.181181994672446
36	21.293060634791637	27.86386142339007	28.961427904946802	21.88165003687149
37	22.49176035035442	27.16736647252698	29.62601772841513	20.714855448703478
38	20.82336298102247	27.470766174545126	28.956762532544733	22.74910831188767
39	22.06781344530227	25.669179947928424	29.352416211416614	22.91059039535269
40	21.339262871160475	25.166824687344803	31.580056285460596	21.913856156034132
41	19.440004815868285	26.251448522882896	30.79717669721733	23.511369964031484
42	21.503152888768494	26.182671903923428	31.39178593465469	20.922389272653394
43	22.730898309931224	25.679714659804056	29.74867187382425	21.84071515644047
44	22.25593330022424	25.861814679368518	28.81078152512529	23.071470495281954
45	21.413457341941697	25.137327494093036	28.192243442141855	25.256971721823408
46	22.80554426836426	25.724110945565638	28.00833747197014	23.46200731409996
47	20.532905925022952	25.607627131397958	30.579710144927535	23.279756798651558
48	20.792059837163453	24.631962315830663	30.198353575029724	24.37762427197616
49	20.680391891281776	24.116363417460533	31.72362935873704	23.479615332520655
50	19.78719882011227	24.69592306650413	30.778816199376948	24.73806191400665
51	19.462579198458922	24.081598868270955	29.08739295980255	27.368428973467573
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	198.0
1	465.5
2	733.0
3	5330.0
4	9927.0
5	6540.0
6	3153.0
7	3070.5
8	2988.0
9	2885.0
10	2782.0
11	2787.5
12	2793.0
13	2715.5
14	2638.0
15	2469.5
16	2301.0
17	2355.0
18	2409.0
19	2329.0
20	2249.0
21	2485.5
22	2722.0
23	3071.5
24	3421.0
25	3880.0
26	5641.0
27	6943.0
28	8349.5
29	9756.0
30	11620.5
31	13485.0
32	14872.5
33	16260.0
34	18658.5
35	21057.0
36	22511.5
37	23966.0
38	25601.5
39	27237.0
40	29792.0
41	32347.0
42	34899.0
43	37451.0
44	41184.5
45	44918.0
46	56239.0
47	67560.0
48	64601.0
49	61642.0
50	59435.0
51	57228.0
52	49492.0
53	41756.0
54	37141.0
55	32526.0
56	29704.0
57	26882.0
58	26165.5
59	25449.0
60	24667.5
61	23886.0
62	21359.5
63	18833.0
64	15507.0
65	12181.0
66	9820.0
67	7459.0
68	6005.0
69	4551.0
70	4083.5
71	3616.0
72	2973.5
73	2331.0
74	1827.5
75	965.0
76	606.0
77	425.0
78	244.0
79	190.5
80	137.0
81	113.0
82	89.0
83	84.5
84	80.0
85	45.0
86	10.0
87	5.0
88	0.0
89	1.5
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	664470.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.073340225745476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.88475497068887	24.493537131606306
2	8.988998145681132	6.125703842127769
3	3.5539576783830844	3.6328562737034207
4	2.2058671197683206	3.006450434586049
5	1.5948655313287832	2.7171197931639983
6	1.3055951420858	2.6691592484021984
7	1.1662781377044933	2.781729424869782
8	1.0336483591711025	2.817588177265643
9	0.8752514494763565	2.6840466378976293
>10	7.304461460786438	42.224813096782334
>50	0.05489194160791049	1.2885818905057962
>100	0.02390455519937529	1.5368560078741056
>500	0.004869446429502374	1.1412162680906353
>1k	0.002213384740682897	1.9795562123211072
>5k	4.426769481365795E-4	0.9007855608032347
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5972	0.8987614188751938	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTC	4767	0.7174138787304167	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCG	2634	0.3964061582915708	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGC	2618	0.3939982241485695	No Hit
GCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	1823	0.27435399641819797	No Hit
GAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT	1282	0.19293572320797028	No Hit
CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT	985	0.1482384456785107	TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	873	0.13138290667750238	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTT	861	0.1295769560702515	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0099176787514863E-4	0.0	0.0	0.15305431396451308	0.0
2	3.0099176787514863E-4	0.0	0.0	0.6903246196216534	0.0
3	4.514876518127229E-4	0.0	0.0	1.0032055623278704	0.0
4	4.514876518127229E-4	0.0	0.0	1.5899890138004726	0.0
5	4.514876518127229E-4	0.0	0.0	3.3632820142369106	0.0
6	4.514876518127229E-4	0.0	0.0	4.386804520896353	0.0
7	4.514876518127229E-4	0.0	0.0	5.323189910755941	0.0
8	4.514876518127229E-4	0.0	0.0	6.668472617273918	0.0
9	4.514876518127229E-4	0.0	0.0	7.293331527382726	0.0
10	4.514876518127229E-4	0.0	0.0	8.662543079446777	0.0
11	4.514876518127229E-4	0.0	0.0	9.859888332054119	0.0
12	4.514876518127229E-4	0.0	0.0	11.206675997411471	0.0
13	4.514876518127229E-4	0.0	0.0	11.659969599831445	0.0
14	4.514876518127229E-4	0.0	0.0	11.879392598612428	0.0
15	4.514876518127229E-4	0.0	0.0	12.20927957620359	0.0
16	4.514876518127229E-4	0.0	0.0	12.700347645491895	0.0
17	4.514876518127229E-4	0.0	0.0	13.348683913494966	0.0
18	4.514876518127229E-4	0.0	0.0	14.026667870633737	0.0
19	4.514876518127229E-4	0.0	0.0	14.55325296853131	0.0
20	4.514876518127229E-4	0.0	0.0	15.037398227158487	0.0
21	4.514876518127229E-4	0.0	0.0	15.54471985191205	0.0
22	4.514876518127229E-4	0.0	0.0	16.204493807094376	0.0
23	4.514876518127229E-4	0.0	0.0	16.782849489066475	0.0
24	4.514876518127229E-4	0.0	0.0	17.246226315710267	0.0
25	4.514876518127229E-4	0.0	0.0	17.63239875389408	0.0
26	4.514876518127229E-4	0.0	0.0	18.01149788553283	0.0
27	4.514876518127229E-4	0.0	0.0	18.395864373109397	0.0
28	4.514876518127229E-4	0.0	0.0	18.81078152512529	0.0
29	4.514876518127229E-4	0.0	0.0	19.226601652444806	0.0
30	4.514876518127229E-4	0.0	0.0	19.71646575466161	0.0
31	6.019835357502973E-4	0.0	0.0	20.15756919048264	0.0
32	6.019835357502973E-4	0.0	0.0	20.625009405992746	0.0
33	6.019835357502973E-4	0.0	0.0	21.040227549776514	0.0
34	6.019835357502973E-4	0.0	0.0	21.453338751185154	0.0
35	7.524794196878715E-4	0.0	0.0	21.886315409273557	0.0
36	7.524794196878715E-4	0.0	0.0	22.32470991918371	0.0
37	7.524794196878715E-4	0.0	0.0	22.763555916745677	0.0
38	7.524794196878715E-4	0.0	0.0	23.18404141646726	0.0
39	7.524794196878715E-4	0.0	0.0	23.61325567745722	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTAG	20	7.031136E-4	45.0	1
TATAGCG	20	7.031136E-4	45.0	1
ACGTAAG	50	2.1827873E-11	45.0	1
TCGACAC	25	3.8890226E-5	45.0	34
ACGGGTC	30	2.1641135E-6	44.999996	5
GCGATAC	30	2.1641135E-6	44.999996	9
CGTTTTT	3255	0.0	42.09677	1
TAGCGAG	60	3.6379788E-12	41.249996	1
GCGATTC	45	1.9261279E-8	40.0	9
GCGATCT	45	1.9261279E-8	40.0	9
TTAACGG	45	1.9261279E-8	40.0	2
ATTGCGG	90	0.0	40.0	2
TCGATCA	40	3.4552795E-7	39.375	17
TACGGCT	350	0.0	39.214287	7
CGTAAGG	75	0.0	39.000004	2
GCTACGA	65	9.094947E-12	38.076927	10
AAGGGCG	215	0.0	37.67442	5
TTTCGCG	30	1.13940354E-4	37.499996	1
TGAACCG	30	1.13940354E-4	37.499996	1
AATGACG	30	1.13940354E-4	37.499996	1
>>END_MODULE