FastQCFastQC Report
Sat 18 Jun 2016
SRR3550657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492552
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC28950.5877552014812649No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCG22760.46208319121635894No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC21430.43508096607058744No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGC20620.418636001884065No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18140.3682859880784161No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC16540.33580210820380385No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGCT12900.26190128148906106No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC6630.13460507723042442No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG6210.1260780587633387No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCT6100.1238447920219591No Hit
ACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC5580.11328753106271013No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCC5390.10943007032759994No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG253.8875478E-545.0000041
TATGACG302.1629658E-645.0000041
CTCTCGC253.8875478E-545.00000411
ACACGAC351.2100463E-745.026
TAGAACG207.029358E-445.01
GACCCGT207.029358E-445.010
AACGGTT207.029358E-445.032
AAAGTCG207.029358E-445.01
TGCGATG207.029358E-445.01
GGTATAC207.029358E-445.08
AGACACG351.2100463E-745.024
CGTGTAA207.029358E-445.020
TATAGCG351.2100463E-745.01
GCGTGTA207.029358E-445.019
GTGCGAT207.029358E-445.023
TAATGCG207.029358E-445.01
TCAACGG207.029358E-445.02
CGTTTTT11950.041.799161
TACGGCT2850.041.052637
CGATAGG556.002665E-1140.9090922