##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550657_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	492552
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29267975767026	33.0	31.0	34.0	30.0	34.0
2	32.434924637398694	34.0	31.0	34.0	30.0	34.0
3	32.41220013318391	34.0	31.0	34.0	30.0	34.0
4	35.92865118809791	37.0	35.0	37.0	35.0	37.0
5	36.008407234160046	37.0	35.0	37.0	35.0	37.0
6	36.04832586204096	37.0	35.0	37.0	35.0	37.0
7	36.10483360132534	37.0	36.0	37.0	35.0	37.0
8	35.989767577839494	37.0	35.0	37.0	35.0	37.0
9	37.85740591856291	39.0	38.0	39.0	35.0	39.0
10	37.56598288099531	39.0	37.0	39.0	35.0	39.0
11	37.54232649547662	39.0	37.0	39.0	35.0	39.0
12	37.45500779613117	39.0	37.0	39.0	35.0	39.0
13	37.40492780457698	39.0	37.0	39.0	35.0	39.0
14	38.66617331774107	40.0	38.0	41.0	35.0	41.0
15	38.73726631908915	40.0	38.0	41.0	35.0	41.0
16	38.81638486900876	40.0	38.0	41.0	35.0	41.0
17	38.76371834851955	40.0	38.0	41.0	35.0	41.0
18	38.69847650603388	40.0	38.0	41.0	35.0	41.0
19	38.6313729311829	40.0	38.0	41.0	35.0	41.0
20	38.53596574574867	40.0	38.0	41.0	35.0	41.0
21	38.48722977472429	40.0	37.0	41.0	35.0	41.0
22	38.45929566827462	40.0	37.0	41.0	34.0	41.0
23	38.4141958615537	40.0	37.0	41.0	34.0	41.0
24	38.38191094544332	40.0	37.0	41.0	34.0	41.0
25	38.31858971235524	40.0	37.0	41.0	34.0	41.0
26	38.3104403189917	40.0	37.0	41.0	34.0	41.0
27	38.29725998473258	40.0	37.0	41.0	34.0	41.0
28	38.220386883009304	40.0	37.0	41.0	34.0	41.0
29	38.19295018597021	40.0	37.0	41.0	34.0	41.0
30	38.11408135587715	40.0	37.0	41.0	34.0	41.0
31	38.02864266107944	40.0	37.0	41.0	34.0	41.0
32	37.91018410238919	40.0	37.0	41.0	34.0	41.0
33	37.81142092611542	40.0	37.0	41.0	34.0	41.0
34	37.69158180253049	40.0	36.0	41.0	33.0	41.0
35	37.62343671328104	40.0	36.0	41.0	33.0	41.0
36	37.54416589517452	40.0	36.0	41.0	33.0	41.0
37	37.47506456171125	40.0	36.0	41.0	33.0	41.0
38	37.396579447449206	40.0	36.0	41.0	33.0	41.0
39	37.317144179700826	40.0	36.0	41.0	33.0	41.0
40	37.23306777761536	40.0	35.0	41.0	33.0	41.0
41	37.15057902515876	40.0	35.0	41.0	32.0	41.0
42	37.120425863665155	40.0	35.0	41.0	32.0	41.0
43	37.057124933002	39.0	35.0	41.0	32.0	41.0
44	36.99455082915103	39.0	35.0	41.0	32.0	41.0
45	36.90728694635287	39.0	35.0	41.0	32.0	41.0
46	36.81387345904595	39.0	35.0	41.0	32.0	41.0
47	36.713863713881985	39.0	35.0	41.0	31.0	41.0
48	36.673193896278974	39.0	35.0	41.0	31.0	41.0
49	36.63401833715019	39.0	35.0	41.0	31.0	41.0
50	36.544774967922166	39.0	35.0	41.0	31.0	41.0
51	35.57235581217821	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	7.0
11	9.0
12	3.0
13	6.0
14	4.0
15	7.0
16	23.0
17	42.0
18	93.0
19	156.0
20	272.0
21	485.0
22	823.0
23	1122.0
24	1793.0
25	2558.0
26	3526.0
27	4047.0
28	4361.0
29	4850.0
30	5681.0
31	7296.0
32	9527.0
33	13198.0
34	24888.0
35	36656.0
36	32174.0
37	48595.0
38	89622.0
39	200500.0
40	219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.8799477009534	22.205980282284916	24.968734265620686	11.945337751140997
2	31.29090938621709	25.817172603095713	25.171149442089362	17.720768568597833
3	30.27274277639721	24.891788075167696	28.37182673098475	16.46364241745034
4	27.490904513635105	24.79210316880248	29.13337068979519	18.583621627767222
5	26.038672058990723	28.917758937127452	25.506545501794736	19.537023502087088
6	25.168104078351117	34.5776689567802	25.370316230570577	14.883910734298105
7	78.29325634653803	5.26624600042229	11.383366629310204	5.057131023729474
8	78.22747648979194	6.1652373759521835	9.877738797121928	5.729547337133948
9	72.02325845799022	7.144017281424094	12.159934382562653	8.67278987802303
10	39.35868700157547	24.834535236888694	19.807045753544802	15.999732007991035
11	29.946482807906577	24.21855966476636	25.39731845571635	20.437639071610715
12	27.90852539427309	20.88571358963115	29.970236645064887	21.235524371030877
13	24.650189218600268	23.1707515145609	32.06463479998051	20.11442446685832
14	20.4250515681593	27.806404196917278	29.37923305559616	22.389311179327258
15	19.019108642336242	25.57151326154396	35.108577368480894	20.300800727638908
16	21.269226396400786	24.712923711608113	31.933887183485194	22.083962708505904
17	21.247299777485424	25.612118111387222	28.67027237733275	24.470309733794604
18	21.887638259513718	25.99380369991392	30.001299355194984	22.11725868537738
19	23.060509347236437	27.548360376163327	27.267577839497147	22.12355243710309
20	25.393460994981243	26.49730383797041	28.090029073072486	20.019206093975864
21	23.161615423346166	28.17326901525118	28.078659715116373	20.58645584628628
22	21.852312040150075	25.436705160064317	27.070644317757314	25.64033848202829
23	22.083759684256687	27.027806201172666	27.674032386428234	23.21440172814241
24	23.418644122854033	24.710690444866735	27.710982799785604	24.159682632493627
25	21.121018694472866	28.118452467962772	26.30544592246098	24.45508291510338
26	20.33592392275333	27.700628563075576	29.28137536747389	22.6820721466972
27	22.871493771216034	26.506236904935925	28.442682193961243	22.179587129886794
28	20.743799639428932	26.520245578131853	29.637073852100894	23.09888093033832
29	21.92519774561874	24.067509623349412	30.734216894865924	23.27307573616593
30	22.10791956991343	24.793524338546995	29.71625330929526	23.38230278224431
31	23.19024184248567	25.13480810147964	26.649978073381085	25.02497198265361
32	23.19125696373175	25.704291120531437	28.42765839951924	22.67679351621758
33	22.092692751222206	24.85849209829622	27.77493543828875	25.273879712192826
34	21.343533271613964	23.955034189283566	28.874108723545937	25.82732381555653
35	20.063871428803456	22.376926700125065	30.07215481817148	27.4870470529
36	21.38048368497133	24.95797398041222	29.45455505205542	24.20698728256103
37	21.68684727703877	24.00416605759392	31.059258717861262	23.24972794750605
38	20.92794263346814	24.202317724829054	27.758490474102228	27.111249167600576
39	22.05472721661875	22.048027416394614	29.439734281862627	26.457511085124008
40	21.442203056733096	21.785923110656334	30.415671847845505	26.35620198476506
41	18.657319430232747	23.798705517387	29.785281554028813	27.758693498351445
42	21.199792103168804	23.204453539930807	31.403790868781368	24.19196348811902
43	22.799623186993454	23.25399145673959	30.55068297357436	23.395702382692587
44	22.27480550276925	23.611720183858758	28.57992658643148	25.533547726940505
45	21.315718949471325	21.630609560005848	28.614237684549042	28.439433805973785
46	22.48493560070815	23.65922785817538	27.96719940229661	25.88863713881986
47	19.877901216521302	22.990262957007584	32.25933505497897	24.87250077149215
48	21.25034514122367	21.4198703893193	30.41912326008218	26.910661209374847
49	21.063968880443078	21.02031866686157	32.00474264646169	25.91096980623366
50	19.931296594065195	21.118176354983838	30.888312300021113	28.062214750929854
51	19.525045071383325	21.171571732527735	28.128197631925154	31.175185564163783
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	157.0
1	316.0
2	475.0
3	1869.0
4	3263.0
5	2280.5
6	1298.0
7	1291.0
8	1284.0
9	1297.0
10	1310.0
11	1319.0
12	1328.0
13	1282.0
14	1236.0
15	1180.5
16	1125.0
17	1115.5
18	1106.0
19	1169.0
20	1232.0
21	1242.0
22	1252.0
23	1498.0
24	1744.0
25	1949.5
26	2494.0
27	2833.0
28	3339.0
29	3845.0
30	4970.5
31	6096.0
32	7081.5
33	8067.0
34	9194.5
35	10322.0
36	11228.0
37	12134.0
38	13832.5
39	15531.0
40	17983.0
41	20435.0
42	22930.5
43	25426.0
44	29028.0
45	32630.0
46	42443.0
47	52256.0
48	54731.5
49	57207.0
50	55163.5
51	53120.0
52	45302.0
53	37484.0
54	32546.0
55	27608.0
56	24725.0
57	21842.0
58	21147.5
59	20453.0
60	19853.0
61	19253.0
62	17663.0
63	16073.0
64	13404.5
65	10736.0
66	9074.5
67	7413.0
68	6105.0
69	4797.0
70	3964.0
71	3131.0
72	2649.5
73	2168.0
74	1727.0
75	1030.0
76	774.0
77	559.5
78	345.0
79	281.0
80	217.0
81	135.5
82	54.0
83	41.0
84	28.0
85	16.5
86	5.0
87	6.0
88	7.0
89	8.5
90	10.0
91	5.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	492552.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.91327478122752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.78724575602223	26.868156031607853
2	10.783818929407794	7.961321426644654
3	3.8262410301301832	4.237172595732076
4	2.0681351823004976	3.053665690759294
5	1.339213994136861	2.4717387078219586
6	1.0193028680408525	2.2575484111971167
7	0.7895399634652583	2.0401153935507392
8	0.696226734870407	2.056000701944654
9	0.5918025842717097	1.966083426851596
>10	5.984135778484894	39.181274656548155
>50	0.07291066478625037	1.826258610853843
>100	0.03479827182980097	2.5890177336856004
>500	0.00276176760553976	0.6098402930739983
>1k	0.0038664746477556637	2.881806319728483
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC	2895	0.5877552014812649	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCG	2276	0.46208319121635894	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	2143	0.43508096607058744	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGC	2062	0.418636001884065	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1814	0.3682859880784161	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	1654	0.33580210820380385	No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGCT	1290	0.26190128148906106	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	663	0.13460507723042442	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG	621	0.1260780587633387	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCT	610	0.1238447920219591	No Hit
ACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	558	0.11328753106271013	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCC	539	0.10943007032759994	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.060484984326528E-4	0.0	0.0	0.2690071302116325	0.0
2	4.060484984326528E-4	0.0	0.0	1.3158001591710113	0.0
3	4.060484984326528E-4	0.0	0.0	1.8456934496256232	0.0
4	4.060484984326528E-4	0.0	0.0	2.555060176387468	0.0
5	4.060484984326528E-4	0.0	0.0	4.879078757166756	0.0
6	4.060484984326528E-4	0.0	0.0	6.19142750410109	0.0
7	4.060484984326528E-4	0.0	0.0	7.415663726875538	0.0
8	4.060484984326528E-4	0.0	0.0	9.308864850817782	0.0
9	4.060484984326528E-4	0.0	0.0	10.115683217203463	0.0
10	4.060484984326528E-4	0.0	0.0	12.185921486462343	0.0
11	4.060484984326528E-4	0.0	0.0	14.320315418473582	0.0
12	4.060484984326528E-4	0.0	0.0	16.258384901492633	0.0
13	4.060484984326528E-4	0.0	0.0	16.9555701733015	0.0
14	4.060484984326528E-4	0.0	0.0	17.245691825431628	0.0
15	4.060484984326528E-4	0.0	0.0	17.744928454254577	0.0
16	4.060484984326528E-4	0.0	0.0	18.683915606880085	0.0
17	4.060484984326528E-4	0.0	0.0	19.758929006480535	0.0
18	4.060484984326528E-4	0.0	0.0	20.919415615001057	0.0
19	4.060484984326528E-4	0.0	0.0	21.709789017200215	0.0
20	4.060484984326528E-4	0.0	0.0	22.392762591563937	0.0
21	4.060484984326528E-4	0.0	0.0	23.250540044502916	0.0
22	4.060484984326528E-4	0.0	0.0	24.18160925140899	0.0
23	4.060484984326528E-4	0.0	0.0	25.116129870551738	0.0
24	4.060484984326528E-4	0.0	0.0	25.797885299420162	0.0
25	4.060484984326528E-4	0.0	0.0	26.433960272214915	0.0
26	4.060484984326528E-4	0.0	0.0	27.017045915964204	0.0
27	4.060484984326528E-4	0.0	0.0	27.604192044697818	0.0
28	4.060484984326528E-4	0.0	0.0	28.203113579885983	0.0
29	4.060484984326528E-4	0.0	0.0	28.825585927983237	0.0
30	4.060484984326528E-4	0.0	0.0	29.507544381100878	0.0
31	4.060484984326528E-4	0.0	0.0	30.16574899706021	0.0
32	6.090727476489792E-4	0.0	0.0	30.79573324237847	0.0
33	6.090727476489792E-4	0.0	0.0	31.3544559762218	0.0
34	6.090727476489792E-4	0.0	0.0	31.897545842875473	0.0
35	6.090727476489792E-4	0.0	0.0	32.45525345547272	0.0
36	6.090727476489792E-4	0.0	0.0	33.06392015462327	0.0
37	6.090727476489792E-4	0.0	0.0	33.644163458883526	0.0
38	6.090727476489792E-4	0.0	0.0	34.25546947327389	0.0
39	6.090727476489792E-4	0.0	0.0	34.82292224983352	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	25	3.8875478E-5	45.000004	1
TATGACG	30	2.1629658E-6	45.000004	1
CTCTCGC	25	3.8875478E-5	45.000004	11
ACACGAC	35	1.2100463E-7	45.0	26
TAGAACG	20	7.029358E-4	45.0	1
GACCCGT	20	7.029358E-4	45.0	10
AACGGTT	20	7.029358E-4	45.0	32
AAAGTCG	20	7.029358E-4	45.0	1
TGCGATG	20	7.029358E-4	45.0	1
GGTATAC	20	7.029358E-4	45.0	8
AGACACG	35	1.2100463E-7	45.0	24
CGTGTAA	20	7.029358E-4	45.0	20
TATAGCG	35	1.2100463E-7	45.0	1
GCGTGTA	20	7.029358E-4	45.0	19
GTGCGAT	20	7.029358E-4	45.0	23
TAATGCG	20	7.029358E-4	45.0	1
TCAACGG	20	7.029358E-4	45.0	2
CGTTTTT	1195	0.0	41.79916	1
TACGGCT	285	0.0	41.05263	7
CGATAGG	55	6.002665E-11	40.909092	2
>>END_MODULE