FastQCFastQC Report
Sat 18 Jun 2016
SRR3550655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423251
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTC35510.8389820697411229No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCG30320.7163597959603166No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGC28890.6825736974041408No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12320.2910802337147461No Hit
GCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC11150.263437062168784No Hit
CCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC7190.16987555847475846No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCT6480.15310064240840543No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6360.15026544532676828No Hit
GAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCT6190.14624891612778232No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5250.12403987232162475No Hit
CTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGCT5160.1219134745103969No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTC4950.11695187961753191No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGCACATTCGTA4950.11695187961753191No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGCACATT4760.11246281757160645No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4250.10041322997464862No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTTC253.8866146E-545.00000445
TCACGGG551.8189894E-1245.03
TACGGGA950.045.04
CGAATTA207.028231E-445.017
GCGATAT207.028231E-445.09
ACGGGAT1300.039.807695
CGGCGAA403.451314E-739.37531
GCATAGG750.039.02
AAGTACG356.238939E-638.571431
ACGTTAG356.238939E-638.571431
TTAGCGG1050.038.571432
CGCGAGG356.238939E-638.571432
TAGCCGT356.238939E-638.5714344
AAAACGC356.238939E-638.5714344
TATTGCG356.238939E-638.571431
TTTGCGC356.238939E-638.5714313
TGGGACG700.038.571436
CTACGGG1000.038.2500043
TACGGCT3350.037.611947
CGGCGCA301.1387034E-437.50000422
Produced by FastQC (version 0.11.5)