##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550655_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	423251
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.340174033847525	33.0	31.0	34.0	30.0	34.0
2	32.491968122934146	34.0	31.0	34.0	31.0	34.0
3	32.46687662876165	34.0	31.0	34.0	30.0	34.0
4	35.96413475691729	37.0	35.0	37.0	35.0	37.0
5	36.022790259207895	37.0	35.0	37.0	35.0	37.0
6	36.04574354224798	37.0	35.0	37.0	35.0	37.0
7	36.05500754871223	37.0	36.0	37.0	35.0	37.0
8	35.91097244897236	37.0	35.0	37.0	35.0	37.0
9	37.78808555679727	39.0	38.0	39.0	35.0	39.0
10	37.566514904867326	39.0	37.0	39.0	35.0	39.0
11	37.485399916361686	39.0	37.0	39.0	35.0	39.0
12	37.34222246373901	39.0	37.0	39.0	35.0	39.0
13	37.252171879097745	39.0	37.0	39.0	34.0	39.0
14	38.515587677288416	40.0	38.0	41.0	34.0	41.0
15	38.58786630155629	40.0	38.0	41.0	35.0	41.0
16	38.67754358524847	40.0	38.0	41.0	35.0	41.0
17	38.60258806240269	40.0	38.0	41.0	35.0	41.0
18	38.54081384332228	40.0	38.0	41.0	35.0	41.0
19	38.50242054950845	40.0	37.0	41.0	34.0	41.0
20	38.374668931674115	40.0	37.0	41.0	34.0	41.0
21	38.35172509929097	40.0	37.0	41.0	34.0	41.0
22	38.3008144103617	40.0	37.0	41.0	34.0	41.0
23	38.248339637709066	40.0	37.0	41.0	34.0	41.0
24	38.215942785722895	40.0	37.0	41.0	34.0	41.0
25	38.17550578734604	40.0	36.0	41.0	34.0	41.0
26	38.14175512875339	40.0	36.0	41.0	34.0	41.0
27	38.12249468991213	40.0	36.0	41.0	34.0	41.0
28	38.040136940019046	40.0	36.0	41.0	34.0	41.0
29	37.98852690247631	40.0	36.0	41.0	34.0	41.0
30	37.93822105559113	40.0	36.0	41.0	34.0	41.0
31	37.87270201369873	40.0	36.0	41.0	34.0	41.0
32	37.72706030227926	40.0	36.0	41.0	33.0	41.0
33	37.63745626117836	40.0	36.0	41.0	33.0	41.0
34	37.537208417700135	40.0	36.0	41.0	33.0	41.0
35	37.43552407436722	40.0	35.0	41.0	33.0	41.0
36	37.38011251007086	40.0	35.0	41.0	33.0	41.0
37	37.33151014409889	40.0	35.0	41.0	33.0	41.0
38	37.24716066825595	40.0	35.0	41.0	33.0	41.0
39	37.165025008800924	40.0	35.0	41.0	33.0	41.0
40	37.04504419363451	40.0	35.0	41.0	32.0	41.0
41	37.00113644149689	39.0	35.0	41.0	32.0	41.0
42	36.948635679537674	39.0	35.0	41.0	32.0	41.0
43	36.899421383528924	39.0	35.0	41.0	32.0	41.0
44	36.87546396818909	39.0	35.0	41.0	32.0	41.0
45	36.77202652799403	39.0	35.0	41.0	32.0	41.0
46	36.69433976529294	39.0	35.0	41.0	31.0	41.0
47	36.56668737935646	39.0	35.0	41.0	31.0	41.0
48	36.544152287885915	39.0	35.0	41.0	31.0	41.0
49	36.50179208082202	39.0	35.0	41.0	31.0	41.0
50	36.39205341511302	39.0	35.0	41.0	31.0	41.0
51	35.49026227935669	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	13.0
11	9.0
12	9.0
13	11.0
14	7.0
15	13.0
16	20.0
17	28.0
18	81.0
19	159.0
20	326.0
21	568.0
22	953.0
23	1264.0
24	1670.0
25	2281.0
26	2943.0
27	3656.0
28	3732.0
29	4295.0
30	5317.0
31	6730.0
32	8530.0
33	11842.0
34	23663.0
35	35544.0
36	26862.0
37	39934.0
38	74029.0
39	168502.0
40	249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.63061634821891	21.666339831447534	24.98824574543238	11.71479807490118
2	31.20890440896773	28.824976196157838	23.250742467235757	16.715376927638683
3	30.428988945094044	27.245771421685948	25.756820420979516	16.56841921224049
4	28.65557317053002	24.109570916548336	28.922554229050846	18.3123016838708
5	28.48451627993791	28.63950705373407	23.3613151534196	19.514661512908415
6	26.08688461456677	35.35538014086204	23.912052186527617	14.645683058043572
7	77.90341901141403	5.935721356830817	11.392294406864957	4.768565224890195
8	77.59461879593906	8.261055496620209	9.159104172228771	4.985221535211966
9	71.35505881852612	7.8551497810991595	10.883140264287622	9.906651136087097
10	42.36918518798538	24.303073117370072	18.379637614559684	14.948104080084867
11	33.15006934419529	22.895870299184175	24.531306482441863	19.422753874178678
12	29.012099203545887	21.67201022561081	28.490422940524653	20.825467630318652
13	24.717720690559503	23.826760007655032	31.151491668064573	20.30402763372089
14	20.455238144741536	29.09975404665317	28.549725812815563	21.89528199578973
15	19.497177797571656	25.054636610427384	34.977117596886956	20.47106799511401
16	22.447909160285505	24.88854131472814	30.558699211578944	22.10485031340741
17	21.99734909072867	25.83691473853576	28.133660641085314	24.032075529650253
18	22.13580121488195	26.170050395628124	28.9190102326988	22.775138156791126
19	24.91996474904962	26.35292060739372	26.347013946806978	22.380100696749682
20	26.668336282725853	26.522559899445007	27.85652012635528	18.952583691473855
21	24.885469851223032	28.22745841120281	26.436795187725487	20.450276549848674
22	22.30355037554548	25.297282227330825	27.667270721156008	24.731896675967686
23	23.641054598807802	26.347013946806978	27.864316918329784	22.14761453605544
24	24.11665890925243	24.65298369052879	28.112396662973033	23.11796073724575
25	22.012942674677674	28.7049528530352	26.10389579705659	23.17820867523054
26	20.98660133112503	28.04576953155456	27.605841451053863	23.361787686266542
27	23.622862084200626	26.981389293823288	27.02651618070601	22.369232441270075
28	20.884061703339153	27.559769498477266	28.60524842233096	22.950920375852625
29	22.380336963173153	26.521851100174597	27.22616130853796	23.87165062811429
30	22.286066660208718	27.706490947451982	26.207616756959816	23.799825635379477
31	25.10756028928461	26.43348745779691	23.536624839634165	24.922327413284314
32	24.943827657820066	28.307552728759056	23.386123127883927	23.36249648553695
33	23.64058206596086	27.516532742982296	24.10626318661976	24.736622004437084
34	21.776676251207913	27.752562900028586	25.192143668886786	25.27861717987672
35	21.34218229844702	27.952680560707478	25.101417362274397	25.60371977857111
36	21.905441451998932	30.03678668213424	25.715710063295777	22.34206180257105
37	22.009162411902157	27.97063680889118	26.33992595410288	23.68027482510378
38	22.54265199609688	28.500818663157325	23.980569449333846	24.975959891411954
39	22.845781817408582	26.35551953805189	26.26526576428644	24.533432880253088
40	21.982936838897015	27.062901209920355	27.880855567972667	23.073306383209964
41	19.446144250102186	27.793200724865386	26.63809418052172	26.122560844510705
42	20.75813169962977	27.052741753711157	28.363311604697923	23.825814941961156
43	23.026761897786418	25.07448298999884	27.60489638535999	24.29385872685475
44	22.26527521494338	25.5748952749078	26.48026820964392	25.6795613005049
45	21.350687889691933	25.2162428440807	26.127994972250505	27.305074293976862
46	22.983761408714923	27.777370874492913	25.763199614413196	23.475668102378965
47	21.00762904281384	25.665385315096717	29.62402924033257	23.70295640175688
48	21.226411750946838	24.95587724541702	27.989774389192227	25.827936614443914
49	21.399831305773642	24.25629236552306	29.71381048125108	24.630065847452222
50	19.891978991189625	24.860189343911767	28.97571417433154	26.272117490567066
51	20.02476072117963	24.64235170147265	26.834904111272035	28.497983466075688
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	154.0
1	471.0
2	788.0
3	1650.5
4	2513.0
5	1769.5
6	1026.0
7	987.5
8	949.0
9	980.5
10	1012.0
11	933.5
12	855.0
13	906.0
14	957.0
15	932.0
16	907.0
17	870.5
18	834.0
19	979.0
20	1124.0
21	1230.5
22	1337.0
23	1407.5
24	1478.0
25	1886.5
26	2909.5
27	3524.0
28	4187.5
29	4851.0
30	6068.0
31	7285.0
32	8123.5
33	8962.0
34	9517.0
35	10072.0
36	10885.5
37	11699.0
38	12544.0
39	13389.0
40	15449.0
41	17509.0
42	19064.5
43	20620.0
44	23589.0
45	26558.0
46	35597.5
47	44637.0
48	41815.5
49	38994.0
50	38287.0
51	37580.0
52	33418.5
53	29257.0
54	26512.5
55	23768.0
56	22540.5
57	21313.0
58	20802.5
59	20292.0
60	19198.5
61	18105.0
62	16842.0
63	15579.0
64	13273.5
65	10968.0
66	9339.5
67	7711.0
68	6616.5
69	5522.0
70	4507.5
71	3493.0
72	2781.0
73	2069.0
74	1779.0
75	1161.5
76	834.0
77	657.5
78	481.0
79	363.5
80	246.0
81	207.0
82	168.0
83	98.5
84	29.0
85	20.0
86	11.0
87	7.0
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	423251.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.788611925317728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.02792232293203	22.17639748187684
2	9.351921242877	5.7586534780614995
3	3.3018283919071267	3.0497613900727316
4	1.8576828308935442	2.2878190304282784
5	1.28289062885425	1.974921085721016
6	1.05049979435186	1.9406058297555298
7	0.8938608728141024	1.9264514869809426
8	0.7938018340199086	1.9552045290595548
9	0.6784976582689319	1.8801000983411091
>10	8.624322506694876	48.89798162830306
>50	0.08298520769509267	1.7913384368587866
>100	0.04533451161120803	2.698126633963875
>500	0.004610289316394037	0.8665706146610851
>1k	0.0038419077636616976	2.7960682759157423
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTC	3551	0.8389820697411229	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCG	3032	0.7163597959603166	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGC	2889	0.6825736974041408	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1232	0.2910802337147461	No Hit
GCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	1115	0.263437062168784	No Hit
CCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	719	0.16987555847475846	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCT	648	0.15310064240840543	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	636	0.15026544532676828	No Hit
GAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCT	619	0.14624891612778232	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	525	0.12403987232162475	No Hit
CTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGCT	516	0.1219134745103969	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTC	495	0.11695187961753191	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGCACATTCGTA	495	0.11695187961753191	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGCACATT	476	0.11246281757160645	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	425	0.10041322997464862	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1256937372859131	0.0
2	0.0	0.0	0.0	0.7475469638583252	0.0
3	0.0	0.0	0.0	1.0667429019659729	0.0
4	0.0	0.0	0.0	1.6663870847322275	0.0
5	0.0	0.0	0.0	3.746476676960007	0.0
6	0.0	0.0	0.0	4.562304637201094	0.0
7	0.0	0.0	0.0	5.414517626656523	0.0
8	0.0	0.0	0.0	6.610025729413516	0.0
9	0.0	0.0	0.0	7.100751090960211	0.0
10	0.0	0.0	0.0	8.692005453029054	0.0
11	0.0	0.0	0.0	9.980129993786193	0.0
12	0.0	0.0	0.0	11.681957042038885	0.0
13	0.0	0.0	0.0	12.1131432648712	0.0
14	0.0	0.0	0.0	12.309716929198041	0.0
15	0.0	0.0	0.0	12.712078648367045	0.0
16	0.0	0.0	0.0	13.307706301934314	0.0
17	0.0	0.0	0.0	13.988626134374165	0.0
18	0.0	0.0	0.0	14.717035517931441	0.0
19	0.0	0.0	0.0	15.307229043758904	0.0
20	0.0	0.0	0.0	15.784723485591293	0.0
21	0.0	0.0	0.0	16.325537328913576	0.0
22	0.0	0.0	0.0	16.909824194154297	0.0
23	0.0	0.0	0.0	17.469775617777632	0.0
24	0.0	0.0	0.0	17.918918088793646	0.0
25	0.0	0.0	0.0	18.341126187534112	0.0
26	0.0	0.0	0.0	18.752702297218434	0.0
27	0.0	0.0	0.0	19.141124297402722	0.0
28	0.0	0.0	0.0	19.536398023867633	0.0
29	0.0	0.0	0.0	19.92789148755703	0.0
30	0.0	0.0	0.0	20.39995180164961	0.0
31	0.0	0.0	0.0	20.83798975076255	0.0
32	0.0	0.0	0.0	21.267758375054047	0.0
33	0.0	0.0	0.0	21.663268367942425	0.0
34	2.3626642346976143E-4	0.0	0.0	22.09350952508086	0.0
35	2.3626642346976143E-4	0.0	0.0	22.523750682219298	0.0
36	2.3626642346976143E-4	0.0	0.0	22.931310262704635	0.0
37	2.3626642346976143E-4	0.0	0.0	23.365567949042056	0.0
38	2.3626642346976143E-4	0.0	0.0	23.755643814190634	0.0
39	7.087992704092843E-4	0.0	0.0	24.186830037022947	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACTTC	25	3.8866146E-5	45.000004	45
TCACGGG	55	1.8189894E-12	45.0	3
TACGGGA	95	0.0	45.0	4
CGAATTA	20	7.028231E-4	45.0	17
GCGATAT	20	7.028231E-4	45.0	9
ACGGGAT	130	0.0	39.80769	5
CGGCGAA	40	3.451314E-7	39.375	31
GCATAGG	75	0.0	39.0	2
AAGTACG	35	6.238939E-6	38.57143	1
ACGTTAG	35	6.238939E-6	38.57143	1
TTAGCGG	105	0.0	38.57143	2
CGCGAGG	35	6.238939E-6	38.57143	2
TAGCCGT	35	6.238939E-6	38.57143	44
AAAACGC	35	6.238939E-6	38.57143	44
TATTGCG	35	6.238939E-6	38.57143	1
TTTGCGC	35	6.238939E-6	38.57143	13
TGGGACG	70	0.0	38.57143	6
CTACGGG	100	0.0	38.250004	3
TACGGCT	335	0.0	37.61194	7
CGGCGCA	30	1.1387034E-4	37.500004	22
>>END_MODULE