FastQCFastQC Report
Sat 18 Jun 2016
SRR3550647_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550647_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346804
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC9790.28229201508633117TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7680.22145073297885837No Hit
CCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC6670.19232765481366998TruSeq Adapter, Index 16 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5320.15340076815723No Hit
CTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGCT5110.14734547467733936TruSeq Adapter, Index 13 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4420.12744951038627006No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA3940.1136088395750914No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3540.10207494723244252No Hit
TCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC3500.10092155799817765TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG207.026466E-445.0000041
GACCGTC207.026466E-445.0000049
GACGTAG207.026466E-445.0000041
CACGATC207.026466E-445.00000412
GGATACC207.026466E-445.0000048
GCGTTAG551.8189894E-1245.0000041
GTCAAGC207.026466E-445.00000416
AATACGG207.026466E-445.0000042
ACCGGCT253.885154E-545.035
CGAATAT453.8380676E-1045.014
TGCAACG253.885154E-545.01
CGCCACC253.885154E-545.020
CTCGTTT302.161103E-644.99999627
ACCTCGT302.161103E-644.99999625
AACGGGC302.161103E-644.9999964
GGACCGT302.161103E-644.9999968
AGGGCCG650.044.9999966
CTAGCTG302.161103E-644.9999961
CGCATGG650.041.538462
AATATAC501.0768417E-940.516
Produced by FastQC (version 0.11.5)