##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550647_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346804
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44697292995467	33.0	31.0	34.0	31.0	34.0
2	32.57518944418173	34.0	31.0	34.0	31.0	34.0
3	32.55541746923334	34.0	31.0	34.0	31.0	34.0
4	36.03034567075351	37.0	35.0	37.0	35.0	37.0
5	36.09788237736589	37.0	35.0	37.0	35.0	37.0
6	36.13218705666601	37.0	36.0	37.0	35.0	37.0
7	36.117619750637246	37.0	37.0	37.0	35.0	37.0
8	36.04823185430387	37.0	36.0	37.0	35.0	37.0
9	37.90207725401091	39.0	38.0	39.0	35.0	39.0
10	37.689248105558185	39.0	37.0	39.0	35.0	39.0
11	37.62477364736277	39.0	37.0	39.0	35.0	39.0
12	37.4666151486142	39.0	37.0	39.0	35.0	39.0
13	37.40593245752644	39.0	37.0	39.0	35.0	39.0
14	38.688475334771226	40.0	38.0	41.0	35.0	41.0
15	38.764201104946885	40.0	38.0	41.0	35.0	41.0
16	38.80722540685805	40.0	38.0	41.0	35.0	41.0
17	38.75407434747004	40.0	38.0	41.0	35.0	41.0
18	38.717684917128985	40.0	38.0	41.0	35.0	41.0
19	38.68615413893727	40.0	38.0	41.0	35.0	41.0
20	38.62233134565922	40.0	38.0	41.0	35.0	41.0
21	38.53531101140702	40.0	37.0	41.0	35.0	41.0
22	38.489524342279786	40.0	37.0	41.0	35.0	41.0
23	38.46278589635644	40.0	37.0	41.0	35.0	41.0
24	38.44271115673406	40.0	37.0	41.0	35.0	41.0
25	38.348216860243824	40.0	37.0	41.0	34.0	41.0
26	38.35640592380711	40.0	37.0	41.0	34.0	41.0
27	38.326351483835246	40.0	37.0	41.0	34.0	41.0
28	38.2460784766035	40.0	37.0	41.0	34.0	41.0
29	38.208264610558125	40.0	37.0	41.0	34.0	41.0
30	38.14979642680015	40.0	37.0	41.0	34.0	41.0
31	38.10056977428172	40.0	36.0	41.0	34.0	41.0
32	38.026677892988545	40.0	36.0	41.0	34.0	41.0
33	37.95788110863773	40.0	36.0	41.0	34.0	41.0
34	37.8517808329777	40.0	36.0	41.0	34.0	41.0
35	37.80980611526972	40.0	36.0	41.0	34.0	41.0
36	37.727174427053896	40.0	36.0	41.0	33.0	41.0
37	37.65518275452417	40.0	36.0	41.0	33.0	41.0
38	37.58551804477457	40.0	35.0	41.0	33.0	41.0
39	37.498713971003795	40.0	35.0	41.0	33.0	41.0
40	37.41484527283422	40.0	35.0	41.0	33.0	41.0
41	37.34866668204519	40.0	35.0	41.0	33.0	41.0
42	37.295253226606384	40.0	35.0	41.0	33.0	41.0
43	37.20915560374159	39.0	35.0	41.0	33.0	41.0
44	37.130877959885126	39.0	35.0	41.0	33.0	41.0
45	37.05379119041303	39.0	35.0	41.0	33.0	41.0
46	36.958160805527044	39.0	35.0	41.0	32.0	41.0
47	36.89209467018835	39.0	35.0	41.0	32.0	41.0
48	36.83944821859033	39.0	35.0	41.0	32.0	41.0
49	36.75794685182409	39.0	35.0	41.0	32.0	41.0
50	36.66051717973264	39.0	35.0	41.0	32.0	41.0
51	35.70754374228671	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	13.0
11	13.0
12	4.0
13	8.0
14	4.0
15	6.0
16	16.0
17	34.0
18	51.0
19	97.0
20	171.0
21	245.0
22	409.0
23	579.0
24	911.0
25	1298.0
26	1840.0
27	2437.0
28	2697.0
29	3132.0
30	3860.0
31	4814.0
32	6385.0
33	8939.0
34	18809.0
35	29683.0
36	22073.0
37	33629.0
38	61228.0
39	143187.0
40	223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.40550858698285	21.478125973172165	24.95184599946944	11.164519440375544
2	31.65130736669704	25.397630938512822	24.78950646474666	18.161555230043483
3	32.78768410975652	24.335071106446293	26.023632945410085	16.8536118383871
4	30.28800129179594	25.59601388680638	25.26239605079526	18.853588770602414
5	26.044682298935424	30.08067957693683	24.100933091890518	19.773705032237228
6	26.56082398126896	34.76718838306363	23.020784074001455	15.651203561665955
7	79.48524238474758	6.742713463512532	8.679830682460409	5.0922134692794785
8	80.22716000968848	6.253099733567087	8.33554399603234	5.184196260712103
9	73.40543938362879	8.860912792239997	10.561296870855008	7.172350953276202
10	40.934648965986554	27.028234968454807	17.114566152639533	14.922549912919115
11	32.91744039861132	24.372556256559903	22.740510490075085	19.969492854753693
12	29.76147910635402	22.623441482797197	27.19288128164612	20.422198129202663
13	25.076123689461483	25.13263976194046	28.972272522808275	20.818964025789782
14	21.045605010322834	27.720556856322304	28.928443731906206	22.30539440144866
15	22.15574214830279	24.247125177333594	33.09073713105962	20.506395543304
16	23.570950738745804	25.186272361333778	29.922953599151104	21.31982330076931
17	22.63411033321415	26.205291749806808	26.372533188775215	24.788064728203825
18	24.77854926702114	26.25978939112582	27.329557905906505	21.63210343594653
19	26.43395116549982	26.164923126607537	25.441748076723453	21.95937763116919
20	27.113874119098973	25.809679242453953	26.710476234414827	20.36597040403225
21	25.38090679461598	26.737580881420055	27.103781963299156	20.777730360664815
22	24.321807130252246	24.9991349580743	25.65945029469095	25.019607616982505
23	24.45502358680984	25.252880589612577	27.19461136549751	23.097484458080068
24	23.563453708723085	23.9423420721791	28.32435612045997	24.169848098637846
25	23.378623083932133	27.000265279523884	26.435392902042654	23.185718734501332
26	22.005801547848353	27.239593545633845	26.95326466822759	23.801340238290216
27	23.990784420018223	26.42328231508287	26.916932907348247	22.66900035755066
28	20.596071556268093	26.596290700222603	29.14326247678804	23.66437526672126
29	22.411506211001026	24.970588574526246	28.757742125235	23.860163089237727
30	24.101798133816217	26.162328000830442	26.86301196064636	22.87286190470698
31	24.96424493373779	26.54381149006355	25.43425104670073	23.05769252949793
32	24.532877360122722	26.338508206364402	26.876275936840404	22.252338496672472
33	23.794131555576058	27.613291657535672	25.33217609946829	23.26040068741998
34	21.547040979919494	25.822654871339427	28.163169974971453	24.467134173769622
35	23.26616763359131	25.33505957255395	27.63289927451817	23.76587351933657
36	24.38553188544538	27.886068211439312	25.192616002122236	22.535783900993067
37	23.34142628112709	27.683936748134393	26.496522531458695	22.47811443927982
38	22.49974048742229	27.441148314321634	25.70356743290158	24.355543765354497
39	23.653418068995745	26.10725366489429	26.440006458979713	23.79932180713025
40	23.14390837475923	24.730683613799147	28.343098695516776	23.782309315924845
41	20.961407596221495	25.35466718953645	27.50083620719484	26.183089007047204
42	23.10873000311415	24.78719968627813	28.191428011210945	23.912642299396776
43	24.405427849736448	24.01702402509775	27.515830267240286	24.06171785792551
44	22.804811939885354	24.8489060103113	27.312545414701102	25.03373663510225
45	21.854707558159653	24.838237159894348	26.326397619404617	26.98065766254138
46	22.900831593637903	26.47835665101902	26.722586821374612	23.898224933968468
47	22.45648839113736	24.369096088857106	29.21909781894096	23.95531770106458
48	21.838560108879943	23.430525599474056	29.646716877544666	25.08419741410134
49	22.964268001522473	23.17216641099872	29.73552784858306	24.128037738895745
50	21.345197863923136	23.89649485011707	29.342222119698736	25.41608516626106
51	21.41613130183043	23.861604825780557	27.36502462486015	27.357239247528863
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	143.0
1	338.5
2	534.0
3	1067.0
4	1600.0
5	1144.5
6	689.0
7	667.0
8	645.0
9	596.5
10	548.0
11	590.0
12	632.0
13	597.0
14	562.0
15	610.0
16	658.0
17	643.5
18	629.0
19	662.0
20	695.0
21	690.0
22	685.0
23	785.5
24	886.0
25	1137.5
26	2083.0
27	2777.0
28	3312.5
29	3848.0
30	4599.5
31	5351.0
32	5947.0
33	6543.0
34	6964.5
35	7386.0
36	7951.0
37	8516.0
38	9500.5
39	10485.0
40	12331.0
41	14177.0
42	15893.5
43	17610.0
44	19967.0
45	22324.0
46	25659.0
47	28994.0
48	31603.0
49	34212.0
50	33561.5
51	32911.0
52	29508.0
53	26105.0
54	23877.0
55	21649.0
56	19854.5
57	18060.0
58	17737.5
59	17415.0
60	16693.5
61	15972.0
62	14554.5
63	13137.0
64	11640.5
65	10144.0
66	8247.0
67	6350.0
68	5774.0
69	5198.0
70	4135.5
71	3073.0
72	2394.5
73	1716.0
74	1531.0
75	1007.0
76	668.0
77	457.0
78	246.0
79	241.5
80	237.0
81	138.0
82	39.0
83	23.5
84	8.0
85	8.0
86	8.0
87	5.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	346804.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.857085750227736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.38187558317692	20.416835230483173
2	10.540174774595974	6.293978041349985
3	3.813784559170985	3.416054778481877
4	2.0764306789344236	2.479846753413947
5	1.4820110641844033	2.2124265713069993
6	1.2174951569920105	2.181051438167846
7	1.0019331138015544	2.094036202633599
8	0.9839694307836343	2.3502767736407795
9	0.8442576605790308	2.268636603047531
>10	9.5113707270663	50.8724021719513
>50	0.10278786954843223	2.092842558833002
>100	0.03891967876105687	2.2915791742574916
>500	0.0049897024052637014	1.0300337024324728
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	979	0.28229201508633117	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	768	0.22145073297885837	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	667	0.19232765481366998	TruSeq Adapter, Index 16 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	532	0.15340076815723	No Hit
CTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGCT	511	0.14734547467733936	TruSeq Adapter, Index 13 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	442	0.12744951038627006	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	394	0.1136088395750914	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	354	0.10207494723244252	No Hit
TCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	350	0.10092155799817765	TruSeq Adapter, Index 16 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8834730856622183E-4	0.0	0.0	0.15224737892296514	0.0
2	2.8834730856622183E-4	0.0	0.0	0.8186180090195038	0.0
3	2.8834730856622183E-4	0.0	0.0	1.1519474977220563	0.0
4	2.8834730856622183E-4	0.0	0.0	1.5833150713371242	0.0
5	2.8834730856622183E-4	0.0	0.0	2.8831847383536524	0.0
6	2.8834730856622183E-4	0.0	0.0	3.6634525553338486	0.0
7	2.8834730856622183E-4	0.0	0.0	4.4169040726173865	0.0
8	2.8834730856622183E-4	0.0	0.0	5.617005570870002	0.0
9	2.8834730856622183E-4	0.0	0.0	6.096238797707062	0.0
10	2.8834730856622183E-4	0.0	0.0	7.106607766923103	0.0
11	2.8834730856622183E-4	0.0	0.0	8.36928063113459	0.0
12	2.8834730856622183E-4	0.0	0.0	9.42463178048696	0.0
13	2.8834730856622183E-4	0.0	0.0	9.906171785792552	0.0
14	2.8834730856622183E-4	0.0	0.0	10.10628481793751	0.0
15	2.8834730856622183E-4	0.0	0.0	10.45662679784547	0.0
16	2.8834730856622183E-4	0.0	0.0	11.160770925364183	0.0
17	2.8834730856622183E-4	0.0	0.0	11.903841939539337	0.0
18	2.8834730856622183E-4	0.0	0.0	12.707465888513397	0.0
19	2.8834730856622183E-4	0.0	0.0	13.225914349315463	0.0
20	2.8834730856622183E-4	0.0	0.0	13.767718942111394	0.0
21	2.8834730856622183E-4	0.0	0.0	14.35046885272373	0.0
22	2.8834730856622183E-4	0.0	0.0	15.009630800106113	0.0
23	2.8834730856622183E-4	0.0	0.0	15.615160148095178	0.0
24	2.8834730856622183E-4	0.0	0.0	16.12841835734305	0.0
25	2.8834730856622183E-4	0.0	0.0	16.59208082951754	0.0
26	2.8834730856622183E-4	0.0	0.0	17.001534007681574	0.0
27	2.8834730856622183E-4	0.0	0.0	17.401760071971488	0.0
28	2.8834730856622183E-4	0.0	0.0	17.82736069941523	0.0
29	2.8834730856622183E-4	0.0	0.0	18.27314563845861	0.0
30	2.8834730856622183E-4	0.0	0.0	18.810336674317483	0.0
31	5.766946171324437E-4	0.0	0.0	19.27659427226906	0.0
32	5.766946171324437E-4	0.0	0.0	19.728434504792332	0.0
33	5.766946171324437E-4	0.0	0.0	20.176237874995675	0.0
34	5.766946171324437E-4	0.0	0.0	20.63067323329604	0.0
35	5.766946171324437E-4	0.0	0.0	21.111059849367365	0.0
36	5.766946171324437E-4	0.0	0.0	21.54675263261093	0.0
37	5.766946171324437E-4	0.0	0.0	22.009261715551148	0.0
38	5.766946171324437E-4	0.0	0.0	22.461966990000114	0.0
39	5.766946171324437E-4	0.0	0.0	22.91870912676901	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	20	7.026466E-4	45.000004	1
GACCGTC	20	7.026466E-4	45.000004	9
GACGTAG	20	7.026466E-4	45.000004	1
CACGATC	20	7.026466E-4	45.000004	12
GGATACC	20	7.026466E-4	45.000004	8
GCGTTAG	55	1.8189894E-12	45.000004	1
GTCAAGC	20	7.026466E-4	45.000004	16
AATACGG	20	7.026466E-4	45.000004	2
ACCGGCT	25	3.885154E-5	45.0	35
CGAATAT	45	3.8380676E-10	45.0	14
TGCAACG	25	3.885154E-5	45.0	1
CGCCACC	25	3.885154E-5	45.0	20
CTCGTTT	30	2.161103E-6	44.999996	27
ACCTCGT	30	2.161103E-6	44.999996	25
AACGGGC	30	2.161103E-6	44.999996	4
GGACCGT	30	2.161103E-6	44.999996	8
AGGGCCG	65	0.0	44.999996	6
CTAGCTG	30	2.161103E-6	44.999996	1
CGCATGG	65	0.0	41.53846	2
AATATAC	50	1.0768417E-9	40.5	16
>>END_MODULE