FastQCFastQC Report
Sat 18 Jun 2016
SRR3550646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296832
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGC13360.4500862440707201No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTC11750.39584680896938335No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCG11100.3739489003880983No Hit
GCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC10660.3591257007330746No Hit
CCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC5880.19809184993531695No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT5590.18832201379905134No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5530.18630066839154807No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5150.1734988141440276No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4720.15901250539025444No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3960.13340879689521346No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3800.12801854247520483No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACAAGT2990.10073037947391117No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG207.024821E-445.0000041
CCTTACC207.024821E-445.00000438
GCCTTAC207.024821E-445.00000437
TTAATCG302.1600426E-645.00000420
CGAAAGG406.7848305E-945.0000042
GCGAATG302.1600426E-645.0000041
TGGACGG207.024821E-445.0000042
CGTTGAT302.1600426E-645.00000425
CCCGTGT207.024821E-445.00000428
GAGCGTA207.024821E-445.0000049
GCGGGTT207.024821E-445.0000045
GACGGTC207.024821E-445.00000429
GCGATCA207.024821E-445.0000049
CGGTCTA207.024821E-445.00000431
AAATGCG207.024821E-445.0000041
AACACGT207.024821E-445.00000441
GTCATAG253.883792E-545.029
CTATGCG253.883792E-545.01
CGTATGG253.883792E-545.02
TTGTGCG253.883792E-545.01