##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550646_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	296832
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43789416235446	33.0	31.0	34.0	31.0	34.0
2	32.57932096269944	34.0	31.0	34.0	31.0	34.0
3	32.56413728978008	34.0	31.0	34.0	31.0	34.0
4	36.04103668068133	37.0	35.0	37.0	35.0	37.0
5	36.09943671841311	37.0	35.0	37.0	35.0	37.0
6	36.133007896722724	37.0	36.0	37.0	35.0	37.0
7	36.110250916343254	37.0	37.0	37.0	35.0	37.0
8	35.98695558430358	37.0	36.0	37.0	35.0	37.0
9	37.86325935209142	39.0	38.0	39.0	35.0	39.0
10	37.712935263044415	39.0	37.0	39.0	35.0	39.0
11	37.62130767572229	39.0	37.0	39.0	35.0	39.0
12	37.43088346269944	39.0	37.0	39.0	35.0	39.0
13	37.3018037138853	39.0	37.0	39.0	34.0	39.0
14	38.58787125377318	40.0	38.0	41.0	35.0	41.0
15	38.683996334627	40.0	38.0	41.0	35.0	41.0
16	38.76245148771022	40.0	38.0	41.0	35.0	41.0
17	38.704516359422165	40.0	38.0	41.0	35.0	41.0
18	38.68774593035791	40.0	38.0	41.0	35.0	41.0
19	38.63973223911169	40.0	38.0	41.0	35.0	41.0
20	38.54795978870202	40.0	37.0	41.0	35.0	41.0
21	38.47854004959034	40.0	37.0	41.0	35.0	41.0
22	38.43148649741268	40.0	37.0	41.0	35.0	41.0
23	38.39634540750323	40.0	37.0	41.0	35.0	41.0
24	38.33252142626132	40.0	37.0	41.0	34.0	41.0
25	38.26544307891332	40.0	37.0	41.0	34.0	41.0
26	38.25875242561449	40.0	37.0	41.0	34.0	41.0
27	38.21369326757223	40.0	37.0	41.0	34.0	41.0
28	38.15143246011212	40.0	36.0	41.0	34.0	41.0
29	38.118255444156965	40.0	36.0	41.0	34.0	41.0
30	38.05784753665373	40.0	36.0	41.0	34.0	41.0
31	38.01093210974558	40.0	36.0	41.0	34.0	41.0
32	37.881370606942646	40.0	36.0	41.0	34.0	41.0
33	37.819092954937474	40.0	36.0	41.0	34.0	41.0
34	37.68427258516602	40.0	36.0	41.0	33.0	41.0
35	37.62330543876671	40.0	35.0	41.0	33.0	41.0
36	37.545096216041394	40.0	35.0	41.0	33.0	41.0
37	37.41648474557999	40.0	35.0	41.0	33.0	41.0
38	37.37533352199224	40.0	35.0	41.0	33.0	41.0
39	37.312314710004316	40.0	35.0	41.0	33.0	41.0
40	37.20572242884864	40.0	35.0	41.0	33.0	41.0
41	37.14601862332902	39.0	35.0	41.0	33.0	41.0
42	37.07824628072445	39.0	35.0	41.0	33.0	41.0
43	37.007563200733074	39.0	35.0	41.0	33.0	41.0
44	36.95339788163001	39.0	35.0	41.0	32.0	41.0
45	36.88147504312204	39.0	35.0	41.0	32.0	41.0
46	36.8179138367831	39.0	35.0	41.0	32.0	41.0
47	36.70898690168176	39.0	35.0	41.0	32.0	41.0
48	36.68522261750755	39.0	35.0	41.0	32.0	41.0
49	36.637825436610605	39.0	35.0	41.0	32.0	41.0
50	36.51576649417853	39.0	35.0	41.0	32.0	41.0
51	35.60203414726175	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	3.0
11	10.0
12	9.0
13	2.0
14	8.0
15	6.0
16	15.0
17	25.0
18	54.0
19	106.0
20	173.0
21	305.0
22	542.0
23	704.0
24	983.0
25	1329.0
26	1715.0
27	2177.0
28	2442.0
29	2892.0
30	3401.0
31	4197.0
32	5499.0
33	7783.0
34	16523.0
35	26882.0
36	18455.0
37	28140.0
38	51450.0
39	120858.0
40	137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.203118262181974	21.218736524363948	23.76900064683053	10.809144566623544
2	31.14286869340233	27.04358020698577	24.30802608883139	17.50552501078051
3	32.77274687365244	25.310950301854245	25.37563335489435	16.540669469598964
4	29.68076218197499	25.7179145105649	25.776533527382494	18.82478978007762
5	27.316798727899954	30.603843251401464	23.172366860715826	18.90699115998275
6	26.359354786545925	36.416221970677014	22.672083872358776	14.552339370418283
7	78.73948900388098	6.771844006037085	9.830476498490729	4.658190491591204
8	78.83381845623113	8.006212268219059	8.17533150064683	4.984637774902975
9	71.55731188012075	9.490553579128935	10.355015631737817	8.597118909012504
10	43.67352576541613	26.058848102630446	16.202431004743424	14.065195127210004
11	34.85439575247952	24.59370957309185	21.842658473479947	18.709236200948684
12	31.408338723587754	22.0643326865028	26.086810047434238	20.440518542475207
13	25.184279322984043	26.238074062095727	27.777665480810693	20.79998113410953
14	19.954048081069427	30.313780185424754	26.878503665373003	22.853668068132816
15	20.351579344545062	25.74452889176369	34.521884432945235	19.382007330746013
16	22.687917744717552	24.69915642518327	31.003395860284606	21.609529969814574
17	21.58662138852954	26.017073630875377	27.629770375161705	24.766534605433378
18	23.83233613626563	26.112076865028026	27.839316515739544	22.216270482966795
19	25.643124730487276	26.83234961190168	25.42145051746442	22.103075140146615
20	27.986874730487276	25.88703374299267	26.652786761535147	19.47330476498491
21	25.77552285467874	26.956325463561882	26.579007654161273	20.689144027598104
22	23.804037300560587	25.592254204398447	25.722967874083658	24.880740620957308
23	25.184279322984043	26.298714424320828	26.34891116860716	22.16809508408797
24	24.22414025442001	24.90769189305735	27.46873652436395	23.39943132815869
25	22.83278083225528	27.128139823199653	26.746105541181542	23.292973803363516
26	21.873652436395	28.50737117291936	26.889957956015525	22.729018434670117
27	24.595730918499353	27.435047434238896	26.750822013799052	21.2183996334627
28	20.222887020267358	28.454142410521776	28.591593898232	22.73137667097887
29	22.484772531263474	26.08108290211298	28.28603385079776	23.148110715825787
30	22.99347779215179	26.839087429926696	26.81280993962915	23.354624838292366
31	24.67490028029323	27.564413540319105	23.545305088400173	24.215381090987496
32	25.509379042690817	28.746900603708497	24.545197283311772	21.198523070288918
33	24.63110446313066	27.790804225959466	23.108357589478224	24.469733721431652
34	22.091620849504096	28.03841903837861	25.890065761103926	23.979894351013368
35	23.658500431220354	27.052002479517036	25.012128072445016	24.277369016817595
36	24.773946205260888	28.4989489003881	24.360581069426477	22.366523824924535
37	24.78876940491591	29.595528783958603	25.240203212591634	20.375498598533852
38	21.558322552824492	29.64505174644243	23.26197984044847	25.534645860284606
39	25.12431274256145	26.627856834842607	25.707807783527382	22.540022639068564
40	23.034578482104358	26.609664726175076	27.629096593359208	22.726660198361362
41	20.63052501078051	28.330840340664082	25.653905239327297	25.384729409228115
42	23.751819210866753	26.41730002156102	26.004945558430357	23.82593520914187
43	25.57406209573092	25.031330853816304	25.34295493747305	24.05165211297973
44	22.746873652436395	26.92735284605433	25.94396830530401	24.38180519620526
45	22.66298781802501	25.929481996550237	24.813362440707202	26.594167744717552
46	22.780225851660198	28.761386912462267	25.202808322552823	23.255578913324708
47	21.877695127210004	26.43953482104355	28.58822498921949	23.09454506252695
48	22.828401250539027	25.00505336351876	27.70759217335058	24.458953212591634
49	22.596620310478656	25.033689090125055	28.782947930142306	23.586742669253987
50	20.85657880551962	26.240432298404485	27.42527759810263	25.477711297973265
51	21.660400495903406	25.61112009486848	24.85480002156102	27.8736793876671
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	108.0
1	352.5
2	597.0
3	897.5
4	1198.0
5	866.0
6	534.0
7	540.5
8	547.0
9	551.0
10	555.0
11	566.0
12	577.0
13	556.5
14	536.0
15	572.5
16	609.0
17	598.0
18	587.0
19	592.0
20	597.0
21	764.5
22	932.0
23	839.5
24	747.0
25	1318.5
26	2066.0
27	2242.0
28	3104.0
29	3966.0
30	4223.0
31	4480.0
32	5039.0
33	5598.0
34	6176.0
35	6754.0
36	6895.5
37	7037.0
38	7640.5
39	8244.0
40	10571.5
41	12899.0
42	14541.5
43	16184.0
44	18013.0
45	19842.0
46	23860.5
47	27879.0
48	27715.0
49	27551.0
50	26782.5
51	26014.0
52	23270.5
53	20527.0
54	18444.0
55	16361.0
56	15698.0
57	15035.0
58	15157.0
59	15279.0
60	14711.5
61	14144.0
62	13357.5
63	12571.0
64	10515.0
65	8459.0
66	7026.5
67	5594.0
68	4689.0
69	3784.0
70	3316.5
71	2849.0
72	2180.5
73	1512.0
74	1201.0
75	753.5
76	617.0
77	412.5
78	208.0
79	211.0
80	214.0
81	144.0
82	74.0
83	41.0
84	8.0
85	4.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	296832.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.827754420008624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.21105527638191	20.81682567917206
2	9.551244595068365	5.5068186718413115
3	3.225429472946126	2.789456662354463
4	1.6360874138132524	1.8865890470030184
5	1.1324062171321725	1.6322364165588614
6	0.8729694986560711	1.509945019404916
7	0.7771415215612949	1.568227145321259
8	0.6439172607222157	1.4850150927123762
9	0.6310622881851116	1.6372897800776196
>10	9.172607222157298	54.70805034497628
>50	0.09115344162673834	1.759918068132816
>100	0.04557672081336917	2.374407072013799
>500	0.004674535468037863	0.746213346269944
>1k	0.004674535468037863	1.5790076541612765
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGC	1336	0.4500862440707201	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTC	1175	0.39584680896938335	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCG	1110	0.3739489003880983	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	1066	0.3591257007330746	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	588	0.19809184993531695	No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT	559	0.18832201379905134	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	553	0.18630066839154807	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	515	0.1734988141440276	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	472	0.15901250539025444	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	396	0.13340879689521346	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	380	0.12801854247520483	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACAAGT	299	0.10073037947391117	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001010672703751617	0.0	0.0	0.19472294092281156	0.0
2	0.001010672703751617	0.0	0.0	0.9527274687365244	0.0
3	0.001010672703751617	0.0	0.0	1.2771534066407935	0.0
4	0.001010672703751617	0.0	0.0	1.7508220137990513	0.0
5	0.001010672703751617	0.0	0.0	3.2189925614489003	0.0
6	0.001010672703751617	0.0	0.0	3.7947391116860714	0.0
7	0.001010672703751617	0.0	0.0	4.4392114057783525	0.0
8	0.001010672703751617	0.0	0.0	5.435734691677447	0.0
9	0.001010672703751617	0.0	0.0	5.77498382923674	0.0
10	0.001010672703751617	0.0	0.0	6.9116537300560585	0.0
11	0.001010672703751617	0.0	0.0	8.044617830961622	0.0
12	0.001010672703751617	0.0	0.0	9.341647800776197	0.0
13	0.001010672703751617	0.0	0.0	9.725703428201811	0.0
14	0.001010672703751617	0.0	0.0	9.885726606295817	0.0
15	0.001010672703751617	0.0	0.0	10.254285252263907	0.0
16	0.001010672703751617	0.0	0.0	10.869448037947391	0.0
17	0.001010672703751617	0.0	0.0	11.577255821474774	0.0
18	0.001010672703751617	0.0	0.0	12.281694695989652	0.0
19	0.001010672703751617	0.0	0.0	12.82509971970677	0.0
20	0.001010672703751617	0.0	0.0	13.309885726606296	0.0
21	0.001010672703751617	0.0	0.0	13.838130659767142	0.0
22	0.001010672703751617	0.0	0.0	14.414887882708063	0.0
23	0.001010672703751617	0.0	0.0	14.955597779215179	0.0
24	0.0013475636050021562	0.0	0.0	15.398946205260888	0.0
25	0.0013475636050021562	0.0	0.0	15.834209249676585	0.0
26	0.0013475636050021562	0.0	0.0	16.183565114273392	0.0
27	0.0013475636050021562	0.0	0.0	16.55717712376024	0.0
28	0.0013475636050021562	0.0	0.0	16.916639715394567	0.0
29	0.001684454506252695	0.0	0.0	17.27643919793014	0.0
30	0.001684454506252695	0.0	0.0	17.73696905993963	0.0
31	0.001684454506252695	0.0	0.0	18.170547649849073	0.0
32	0.002021345407503234	0.0	0.0	18.57077404053471	0.0
33	0.002021345407503234	0.0	0.0	18.93124730487279	0.0
34	0.002358236308753773	0.0	0.0	19.31900873221216	0.0
35	0.002358236308753773	0.0	0.0	19.7192351228978	0.0
36	0.002358236308753773	0.0	0.0	20.128557567917206	0.0
37	0.002358236308753773	0.0	0.0	20.529120849504096	0.0
38	0.002358236308753773	0.0	0.0	20.940464639931005	0.0
39	0.002358236308753773	0.0	0.0	21.352482212160414	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	20	7.024821E-4	45.000004	1
CCTTACC	20	7.024821E-4	45.000004	38
GCCTTAC	20	7.024821E-4	45.000004	37
TTAATCG	30	2.1600426E-6	45.000004	20
CGAAAGG	40	6.7848305E-9	45.000004	2
GCGAATG	30	2.1600426E-6	45.000004	1
TGGACGG	20	7.024821E-4	45.000004	2
CGTTGAT	30	2.1600426E-6	45.000004	25
CCCGTGT	20	7.024821E-4	45.000004	28
GAGCGTA	20	7.024821E-4	45.000004	9
GCGGGTT	20	7.024821E-4	45.000004	5
GACGGTC	20	7.024821E-4	45.000004	29
GCGATCA	20	7.024821E-4	45.000004	9
CGGTCTA	20	7.024821E-4	45.000004	31
AAATGCG	20	7.024821E-4	45.000004	1
AACACGT	20	7.024821E-4	45.000004	41
GTCATAG	25	3.883792E-5	45.0	29
CTATGCG	25	3.883792E-5	45.0	1
CGTATGG	25	3.883792E-5	45.0	2
TTGTGCG	25	3.883792E-5	45.0	1
>>END_MODULE