FastQCFastQC Report
Sat 18 Jun 2016
SRR3550645_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550645_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769540
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50980.6624736855783975No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC23530.3057670816331835No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG22800.29628089508017774No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC22730.2953712607531772No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC18010.2340359175611404RNA PCR Primer, Index 40 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT11980.1556774176780934Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC10460.13592535800608155Illumina PCR Primer Index 8 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTT10320.13410608935208046No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC9860.12812849234607687RNA PCR Primer, Index 40 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCCG207.0318306E-445.00000436
TCTAGCG207.0318306E-445.0000041
TCGCTAG207.0318306E-445.00000437
CGGTCTA207.0318306E-445.00000431
ATACGCG207.0318306E-445.0000041
CGTCCGT207.0318306E-445.00000413
TGTCGCG207.0318306E-445.0000041
TTTAGCG502.1827873E-1145.01
CGTGCGG453.8380676E-1045.02
TTTCGCG253.8895996E-545.01
GTTGTCG253.8895996E-545.038
CGCAATC253.8895996E-545.020
TGGTACG253.8895996E-545.01
CAACGAC302.1645628E-644.99999612
CGTTTTT25050.043.2035941
CGACAGG700.041.7857132
CGCGACC451.9268555E-840.010
ACTACGG451.9268555E-840.02
TATACGG900.040.02
TATTACG403.4562254E-739.3750041
Produced by FastQC (version 0.11.5)