##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550645_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	769540
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41742729422772	33.0	31.0	34.0	31.0	34.0
2	32.576939470332924	34.0	31.0	34.0	31.0	34.0
3	32.52420406996387	34.0	31.0	34.0	31.0	34.0
4	36.010193102372845	37.0	35.0	37.0	35.0	37.0
5	36.096794188736126	37.0	35.0	37.0	35.0	37.0
6	36.14345972918887	37.0	36.0	37.0	35.0	37.0
7	36.22385321100917	37.0	37.0	37.0	35.0	37.0
8	36.16893988616576	37.0	37.0	37.0	35.0	37.0
9	38.04537905761884	39.0	39.0	39.0	37.0	39.0
10	37.75211944798191	39.0	38.0	39.0	35.0	39.0
11	37.65779946461522	39.0	37.0	39.0	35.0	39.0
12	37.67250175429477	39.0	37.0	39.0	35.0	39.0
13	37.707451204615744	39.0	37.0	39.0	35.0	39.0
14	39.065138914156506	40.0	39.0	41.0	35.0	41.0
15	39.11820828027133	40.0	39.0	41.0	35.0	41.0
16	39.16394599371053	40.0	39.0	41.0	35.0	41.0
17	39.109432907971	40.0	39.0	41.0	35.0	41.0
18	39.02163760168412	40.0	38.0	41.0	35.0	41.0
19	38.96494009408218	40.0	38.0	41.0	35.0	41.0
20	38.882801413831636	40.0	38.0	41.0	35.0	41.0
21	38.824935675858306	40.0	38.0	41.0	35.0	41.0
22	38.802648335369184	40.0	38.0	41.0	35.0	41.0
23	38.760948098864255	40.0	38.0	41.0	35.0	41.0
24	38.7552109052161	40.0	38.0	41.0	35.0	41.0
25	38.68608649322972	40.0	38.0	41.0	35.0	41.0
26	38.71582893676742	40.0	38.0	41.0	35.0	41.0
27	38.69694882657172	40.0	38.0	41.0	35.0	41.0
28	38.64708397224316	40.0	38.0	41.0	35.0	41.0
29	38.61500506796268	40.0	38.0	41.0	35.0	41.0
30	38.52877823115108	40.0	38.0	41.0	35.0	41.0
31	38.43710268472074	40.0	38.0	41.0	35.0	41.0
32	38.32528913376822	40.0	38.0	41.0	34.0	41.0
33	38.189135067702786	40.0	38.0	41.0	34.0	41.0
34	38.058467396106764	40.0	38.0	41.0	34.0	41.0
35	37.9904358447904	40.0	38.0	41.0	34.0	41.0
36	37.9173727161681	40.0	38.0	41.0	34.0	41.0
37	37.88153442316189	40.0	38.0	41.0	34.0	41.0
38	37.83296254905528	40.0	37.0	41.0	34.0	41.0
39	37.76208644125062	40.0	37.0	41.0	33.0	41.0
40	37.68801231904774	40.0	37.0	41.0	33.0	41.0
41	37.64814044754009	40.0	37.0	41.0	33.0	41.0
42	37.627329313615924	40.0	37.0	41.0	33.0	41.0
43	37.57705642331783	40.0	37.0	41.0	33.0	41.0
44	37.52404943212829	40.0	37.0	41.0	33.0	41.0
45	37.43129921771448	40.0	37.0	41.0	33.0	41.0
46	37.36976245549289	40.0	36.0	41.0	33.0	41.0
47	37.29791823686878	40.0	36.0	41.0	33.0	41.0
48	37.28271045039894	40.0	36.0	41.0	33.0	41.0
49	37.260197000805675	40.0	36.0	41.0	33.0	41.0
50	37.179097902643136	40.0	36.0	41.0	32.0	41.0
51	36.33357070457676	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	7.0
10	17.0
11	6.0
12	7.0
13	12.0
14	11.0
15	12.0
16	31.0
17	36.0
18	89.0
19	168.0
20	281.0
21	535.0
22	877.0
23	1377.0
24	2159.0
25	3588.0
26	5379.0
27	6556.0
28	6859.0
29	7154.0
30	7904.0
31	9753.0
32	12105.0
33	16133.0
34	29111.0
35	44323.0
36	41563.0
37	62708.0
38	132072.0
39	378140.0
40	558.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.79863294955428	21.87982431062713	26.73038438547704	12.591158354341555
2	32.18858019076331	25.07900823868805	26.10143722223666	16.630974348311977
3	30.133976141591077	24.54596252306573	29.51178626192271	15.808275073420486
4	26.905813862827145	25.709384827299424	30.24716064142215	17.137640668451283
5	26.208514177300724	29.1696338072095	26.538711438001922	18.08314057748785
6	25.61998076773137	34.5030797619357	25.191932842997115	14.685006627335811
7	81.3215687293708	4.723341216830834	9.948280791121968	4.006809262676405
8	83.46388751721808	4.735296410842841	8.346934532318008	3.4538815396210722
9	76.657483691556	7.759570652597655	9.871351716609924	5.711593939236427
10	32.713179301920626	41.557683811107935	15.764092834680458	9.96504405229098
11	22.3321724666684	23.58447903942615	36.06076357304364	18.022584920861814
12	22.124775840112274	22.56958702601554	36.14834836395769	19.157288769914494
13	21.38134469943083	23.289237726433974	37.273176183174364	18.056241390960835
14	18.708839046703226	27.727213660108635	33.49234607687709	20.071601216311045
15	17.46783792915248	27.944096473217765	36.1731683863087	18.414897211321048
16	21.34586896067781	27.280453257790366	32.710060555656625	18.6636172258752
17	21.323777841307795	26.89827689268914	31.278296124957766	20.4996491410453
18	20.325779036827196	27.343607869636404	32.41520908594745	19.91540400758895
19	20.54928918574733	28.107050965511863	31.76157184811706	19.58208800062375
20	22.61623827221457	27.796735712243674	31.575746549886947	18.011279465654805
21	22.226394989214334	28.339917353224003	31.786521818229076	17.647165839332587
22	21.10143722223666	24.506978194765704	32.52709410816851	21.86449047482912
23	19.265535254827558	27.40130467552044	32.58855939912155	20.744600670530446
24	20.31863190997219	26.820438183850094	32.59713595134755	20.26379395483016
25	19.257348545884554	29.4977519037347	30.737843386958442	20.507056163422305
26	18.874782337500324	29.413675702367648	30.856225797229513	20.85531616290251
27	19.990253917924996	28.256360942900955	31.108844244613664	20.644540894560386
28	18.090157756581853	28.059620032746835	33.0019232268628	20.848298983808508
29	19.946331574706967	25.303428021935183	32.78724952569067	21.962990877667178
30	20.058216596928034	26.959352340359178	31.55066663201393	21.43176443069886
31	20.70184785716142	28.337968136809007	29.57507082152975	21.385113184499833
32	21.87150765392312	28.106661122228864	28.91467630012735	21.107154923720664
33	20.273540036905164	28.905190113574342	28.41190841281805	22.409361436702447
34	19.53270785144372	28.359279569613015	29.978558619434985	22.12945395950828
35	19.17924994152351	27.224315825038335	29.807677313719882	23.788756919718274
36	19.490864672401695	28.907139329989345	29.055149829768435	22.546846167840528
37	20.003248694025004	28.770824128700262	29.281388881669567	21.944538295605167
38	19.43056891129766	29.3379161577046	27.921745458325752	23.309769472671984
39	21.171738960937702	27.996985211944796	28.507809860436105	22.323465966681393
40	19.945551888140965	27.896405644930738	29.91254515684695	22.245497310081348
41	17.96865660004678	28.31080905475999	29.34454349351561	24.375990851677624
42	19.48176832913169	26.76430075109806	30.14476180575409	23.609169114016165
43	20.774358707799465	25.73056631234244	29.785196351066872	23.709878628791227
44	20.631286222938378	26.64539854978299	29.468513657509682	23.254801569768954
45	20.056787171557033	26.648907139329992	28.96353665826338	24.3307690308496
46	21.502585960443902	27.53645034696052	28.510278867895106	22.45068482470047
47	18.721963770564233	26.46230215453388	31.350677027834813	23.465057047067077
48	19.140265613223484	25.962393117966577	30.29693063388518	24.60041063492476
49	19.966083634378982	24.939444343373964	31.314551550276786	23.779920471970268
50	18.982119188086386	25.464563245575278	30.63414507368038	24.91917249265795
51	18.58850742001715	24.963614626919977	29.462146217220674	26.98573173584219
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	179.0
1	435.5
2	692.0
3	4597.0
4	8502.0
5	5562.5
6	2623.0
7	2542.5
8	2462.0
9	2463.0
10	2464.0
11	2401.5
12	2339.0
13	2336.5
14	2334.0
15	2295.0
16	2256.0
17	2247.0
18	2238.0
19	2558.0
20	2878.0
21	3369.0
22	3860.0
23	4237.0
24	4614.0
25	5729.0
26	8422.0
27	10000.0
28	11713.0
29	13426.0
30	14901.5
31	16377.0
32	19884.5
33	23392.0
34	26353.5
35	29315.0
36	31774.0
37	34233.0
38	36274.5
39	38316.0
40	42702.5
41	47089.0
42	49651.0
43	52213.0
44	55761.0
45	59309.0
46	69874.0
47	80439.0
48	79875.5
49	79312.0
50	72678.0
51	66044.0
52	56471.0
53	46898.0
54	40508.0
55	34118.0
56	30041.5
57	25965.0
58	23819.0
59	21673.0
60	19072.0
61	16471.0
62	14512.0
63	12553.0
64	10382.5
65	8212.0
66	6360.0
67	4508.0
68	3434.0
69	2360.0
70	1882.0
71	1404.0
72	1127.5
73	851.0
74	594.5
75	252.0
76	166.0
77	174.0
78	182.0
79	113.0
80	44.0
81	39.5
82	35.0
83	21.5
84	8.0
85	5.0
86	2.0
87	1.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	769540.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.867987575743545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.11734134956815	22.663665505479504
2	10.304401834431802	6.567610992702832
3	3.6957478892793687	3.5332814345590604
4	1.9256384964475022	2.4546489472064987
5	1.3368338799432504	2.130110273843227
6	1.0241631480169031	1.9582811085922236
7	0.9325217842066804	2.0802314843246084
8	0.7649984489824213	1.9503168854107906
9	0.7167722328717251	2.0557879750614685
>10	8.074794277653101	48.62343188692614
>50	0.07405511589229231	1.6209593767037165
>100	0.02741266701693159	1.6665944852118302
>500	0.0020457214191739997	0.4679555317966874
>1k	0.002864009986843599	1.5624160316298983
>5k	4.0914428383479986E-4	0.6647080805515498
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5098	0.6624736855783975	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	2353	0.3057670816331835	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	2280	0.29628089508017774	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	2273	0.2953712607531772	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	1801	0.2340359175611404	RNA PCR Primer, Index 40 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT	1198	0.1556774176780934	Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	1046	0.13592535800608155	Illumina PCR Primer Index 8 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTT	1032	0.13410608935208046	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	986	0.12812849234607687	RNA PCR Primer, Index 40 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5989552200015594E-4	0.0	0.0	0.16256464901109755	0.0
2	2.5989552200015594E-4	0.0	0.0	0.6806663721184084	0.0
3	2.5989552200015594E-4	0.0	0.0	1.0038464537256022	0.0
4	2.5989552200015594E-4	0.0	0.0	1.4937495126958962	0.0
5	2.5989552200015594E-4	0.0	0.0	2.990227928372794	0.0
6	2.5989552200015594E-4	0.0	0.0	3.9042804792473427	0.0
7	2.5989552200015594E-4	0.0	0.0	4.7438729630688465	0.0
8	2.5989552200015594E-4	0.0	0.0	5.968370714972581	0.0
9	2.5989552200015594E-4	0.0	0.0	6.491410452997894	0.0
10	2.5989552200015594E-4	0.0	0.0	7.720976167580632	0.0
11	2.5989552200015594E-4	0.0	0.0	9.027600904436417	0.0
12	2.5989552200015594E-4	0.0	0.0	10.430386984432259	0.0
13	2.5989552200015594E-4	0.0	0.0	10.88351482703953	0.0
14	3.898432830002339E-4	0.0	0.0	11.077007043168646	0.0
15	3.898432830002339E-4	0.0	0.0	11.419289445642852	0.0
16	3.898432830002339E-4	0.0	0.0	11.982872885100189	0.0
17	3.898432830002339E-4	0.0	0.0	12.844036697247706	0.0
18	3.898432830002339E-4	0.0	0.0	13.68193986017621	0.0
19	3.898432830002339E-4	0.0	0.0	14.295033396574578	0.0
20	3.898432830002339E-4	0.0	0.0	14.834706448007902	0.0
21	3.898432830002339E-4	0.0	0.0	15.473269745562284	0.0
22	3.898432830002339E-4	0.0	0.0	16.235543311588742	0.0
23	3.898432830002339E-4	0.0	0.0	16.924526340411155	0.0
24	3.898432830002339E-4	0.0	0.0	17.454193414247474	0.0
25	3.898432830002339E-4	0.0	0.0	17.911869428489748	0.0
26	3.898432830002339E-4	0.0	0.0	18.345115263664006	0.0
27	3.898432830002339E-4	0.0	0.0	18.803700912233282	0.0
28	3.898432830002339E-4	0.0	0.0	19.270083426462563	0.0
29	3.898432830002339E-4	0.0	0.0	19.791563791355873	0.0
30	3.898432830002339E-4	0.0	0.0	20.412714088936248	0.0
31	3.898432830002339E-4	0.0	0.0	20.959404319463577	0.0
32	3.898432830002339E-4	0.0	0.0	21.46399147542688	0.0
33	3.898432830002339E-4	0.0	0.0	21.953114847831174	0.0
34	3.898432830002339E-4	0.0	0.0	22.437819996361462	0.0
35	3.898432830002339E-4	0.0	0.0	22.959690204537775	0.0
36	3.898432830002339E-4	0.0	0.0	23.473243756010085	0.0
37	3.898432830002339E-4	0.0	0.0	23.974842113470384	0.0
38	3.898432830002339E-4	0.0	0.0	24.46591470228968	0.0
39	5.197910440003119E-4	0.0	0.0	24.96400447020298	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCCCG	20	7.0318306E-4	45.000004	36
TCTAGCG	20	7.0318306E-4	45.000004	1
TCGCTAG	20	7.0318306E-4	45.000004	37
CGGTCTA	20	7.0318306E-4	45.000004	31
ATACGCG	20	7.0318306E-4	45.000004	1
CGTCCGT	20	7.0318306E-4	45.000004	13
TGTCGCG	20	7.0318306E-4	45.000004	1
TTTAGCG	50	2.1827873E-11	45.0	1
CGTGCGG	45	3.8380676E-10	45.0	2
TTTCGCG	25	3.8895996E-5	45.0	1
GTTGTCG	25	3.8895996E-5	45.0	38
CGCAATC	25	3.8895996E-5	45.0	20
TGGTACG	25	3.8895996E-5	45.0	1
CAACGAC	30	2.1645628E-6	44.999996	12
CGTTTTT	2505	0.0	43.203594	1
CGACAGG	70	0.0	41.785713	2
CGCGACC	45	1.9268555E-8	40.0	10
ACTACGG	45	1.9268555E-8	40.0	2
TATACGG	90	0.0	40.0	2
TATTACG	40	3.4562254E-7	39.375004	1
>>END_MODULE