##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550643_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	213611
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39540566731114	33.0	31.0	34.0	31.0	34.0
2	32.53482264490125	34.0	31.0	34.0	31.0	34.0
3	32.50990351620469	34.0	31.0	34.0	31.0	34.0
4	35.985646806578316	37.0	35.0	37.0	35.0	37.0
5	36.055432538586494	37.0	35.0	37.0	35.0	37.0
6	36.08840368707604	37.0	35.0	37.0	35.0	37.0
7	36.09518236420409	37.0	36.0	37.0	35.0	37.0
8	36.000884785895856	37.0	36.0	37.0	35.0	37.0
9	37.84592553754254	39.0	38.0	39.0	35.0	39.0
10	37.657442734690626	39.0	37.0	39.0	35.0	39.0
11	37.590531386492266	39.0	37.0	39.0	35.0	39.0
12	37.45118463000501	39.0	37.0	39.0	35.0	39.0
13	37.37029460093347	39.0	37.0	39.0	35.0	39.0
14	38.626802926815564	40.0	38.0	41.0	35.0	41.0
15	38.739952530534474	40.0	38.0	41.0	35.0	41.0
16	38.79997752924709	40.0	38.0	41.0	35.0	41.0
17	38.776518999489724	40.0	38.0	41.0	35.0	41.0
18	38.731863059486635	40.0	38.0	41.0	35.0	41.0
19	38.70337669876551	40.0	38.0	41.0	35.0	41.0
20	38.63721905707103	40.0	38.0	41.0	35.0	41.0
21	38.55138078095229	40.0	38.0	41.0	35.0	41.0
22	38.51299792613676	40.0	38.0	41.0	35.0	41.0
23	38.49526943837162	40.0	37.0	41.0	35.0	41.0
24	38.45916174728829	40.0	37.0	41.0	35.0	41.0
25	38.340520853326844	40.0	37.0	41.0	34.0	41.0
26	38.356774697932224	40.0	37.0	41.0	34.0	41.0
27	38.33517000528999	40.0	37.0	41.0	34.0	41.0
28	38.272996240830295	40.0	37.0	41.0	34.0	41.0
29	38.26582900693317	40.0	37.0	41.0	34.0	41.0
30	38.194222207657845	40.0	37.0	41.0	34.0	41.0
31	38.13452490742518	40.0	37.0	41.0	34.0	41.0
32	38.02793395471207	40.0	37.0	41.0	34.0	41.0
33	37.96169672910103	40.0	36.0	41.0	34.0	41.0
34	37.90327745294016	40.0	36.0	41.0	34.0	41.0
35	37.821788203791	40.0	36.0	41.0	34.0	41.0
36	37.73876345319295	40.0	36.0	41.0	33.0	41.0
37	37.65560294179607	40.0	36.0	41.0	33.0	41.0
38	37.60311500812224	40.0	36.0	41.0	33.0	41.0
39	37.533057754516385	40.0	36.0	41.0	33.0	41.0
40	37.44070296005356	40.0	35.0	41.0	33.0	41.0
41	37.36716273974655	40.0	35.0	41.0	33.0	41.0
42	37.33736090369878	40.0	35.0	41.0	33.0	41.0
43	37.263703648220364	39.0	35.0	41.0	33.0	41.0
44	37.18868410334674	39.0	35.0	41.0	33.0	41.0
45	37.12325676112185	39.0	35.0	41.0	33.0	41.0
46	37.03379507609627	39.0	35.0	41.0	32.0	41.0
47	36.94823300298206	39.0	35.0	41.0	32.0	41.0
48	36.92018669450543	39.0	35.0	41.0	32.0	41.0
49	36.84177312966093	39.0	35.0	41.0	32.0	41.0
50	36.74653926998141	39.0	35.0	41.0	32.0	41.0
51	35.77630365477433	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	4.0
11	6.0
12	5.0
13	5.0
14	6.0
15	5.0
16	11.0
17	19.0
18	36.0
19	44.0
20	91.0
21	171.0
22	263.0
23	415.0
24	598.0
25	856.0
26	1195.0
27	1446.0
28	1599.0
29	1867.0
30	2356.0
31	2944.0
32	3930.0
33	5586.0
34	11166.0
35	17807.0
36	13609.0
37	20350.0
38	38127.0
39	88954.0
40	129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.19029450730534	21.8415718291661	25.022587788082074	11.94554587544649
2	32.37286469329763	24.93504547986761	24.279648519973225	18.41244130686154
3	32.01520520946955	24.43553936829096	26.419987734714034	17.129267687525456
4	28.961991657732984	25.966359410329993	25.2566581309015	19.814990801035528
5	25.58107962604922	29.91746679712187	24.078348025148518	20.42310555168039
6	26.66154832850368	33.83112292906264	23.92667044300153	15.580658299432146
7	78.53247257865934	6.219717149397737	9.417586172996709	5.830224098946216
8	77.76846697969674	6.374671716344197	9.475167477330288	6.381693826628779
9	70.98136331930472	8.854412928173176	12.42539007822631	7.738833674295799
10	40.19783625375098	25.77395358853243	19.073455954983594	14.954754202733007
11	31.022278815229555	24.65790619396941	24.37327665710099	19.946538333700044
12	30.138897341429043	21.69925706073189	28.64318785081293	19.518657747026136
13	25.013224974369297	24.656501771912495	29.627687712711428	20.702585541006783
14	19.40864468590101	29.33744048761534	28.989611958185673	22.26430286829798
15	19.050985202072926	25.6470874627243	34.79455646010739	20.507370875095386
16	20.830856088871826	25.289427978896217	32.72350206684113	21.15621386539083
17	20.623469765133816	25.062847887047013	28.27148414641568	26.042198201403487
18	21.710024296501583	25.9303125775358	30.094892116978993	22.26477100898362
19	24.199596462728977	26.769688826886256	26.963030930055098	22.067683780329663
20	26.189194376694086	25.798765044871285	27.241106497324573	20.770934081110052
21	24.685058353736462	26.094629958195036	27.688180852109678	21.532130835958824
22	22.762404557817714	25.796424341443092	25.99912925832471	25.442041842414483
23	22.786279732785296	26.765943701401145	26.940092036458797	23.507684529354762
24	23.76750260988432	24.001572952703746	28.585138405793707	23.645786031618222
25	22.05317141907486	26.809948925851195	26.96630791485457	24.170571740219373
26	21.04947778906517	28.22139309305232	27.454110509290253	23.275018608592255
27	22.63787913543778	27.191483584646857	27.44334327352056	22.727294006394803
28	19.717617538422648	27.335670915823624	29.32901395527384	23.617697590479892
29	21.371558580784697	25.26789350735683	29.456348221767602	23.904199690090866
30	22.75631872890441	25.104512408068874	27.15028720431064	24.98888165871608
31	23.392990061373244	27.587530604697324	25.728543942025457	23.290935391903975
32	25.344668579801606	27.422276942666812	25.400377321392625	21.832677156138963
33	24.65790619396941	27.525736034192995	24.832522669712702	22.983835102124893
34	23.573224225344198	25.84511097274953	28.037881944281896	22.54378285762437
35	24.74685292424079	24.944408293580388	25.986021319126824	24.322717463051998
36	24.16635847404862	27.671327787426677	26.23085889771594	21.931454840808758
37	24.00297737476066	27.558974022873358	27.60953321692235	20.828515385443634
38	23.75252210794388	27.642771205602706	25.778634995388817	22.826071691064598
39	25.835748159036754	25.827789767380892	26.596944913885523	21.739517159696835
40	26.356320601467154	23.761416780971018	28.373070675199312	21.50919194236252
41	21.905238962412984	25.95606031524594	27.41431855101095	24.724382171330127
42	22.72823028776608	26.045943326888597	27.886204362134908	23.339622023210417
43	23.5479446283197	25.476216112466116	28.131510081409665	22.844329177804514
44	22.673925968231973	25.948101923590077	27.46347332300303	23.91449878517492
45	23.037671280973356	24.400428816868043	26.110546741506756	26.45135316065184
46	24.473458763827704	25.54035138639864	26.679337674557956	23.306852175215695
47	22.699205565256474	25.06846557527468	29.90669956135218	22.32562929811667
48	23.229608962085287	23.571351662601643	28.449377606958443	24.749661768354624
49	22.507736024830184	24.19912832204334	29.807921876682382	23.4852137764441
50	21.74466670723886	23.637359499276723	29.4418358605128	25.176137932971614
51	21.514809630590186	23.375668856004605	27.12360318522922	27.985918328175984
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	79.0
1	192.5
2	306.0
3	567.5
4	829.0
5	599.5
6	370.0
7	393.0
8	416.0
9	416.0
10	416.0
11	411.5
12	407.0
13	379.0
14	351.0
15	371.5
16	392.0
17	410.0
18	428.0
19	433.0
20	438.0
21	485.5
22	533.0
23	611.5
24	690.0
25	855.5
26	1320.5
27	1620.0
28	1973.0
29	2326.0
30	2833.0
31	3340.0
32	3616.5
33	3893.0
34	4416.5
35	4940.0
36	5516.0
37	6092.0
38	6502.5
39	6913.0
40	7876.0
41	8839.0
42	10109.0
43	11379.0
44	13113.5
45	14848.0
46	16829.5
47	18811.0
48	20659.5
49	22508.0
50	22306.0
51	22104.0
52	18862.5
53	15621.0
54	13763.5
55	11906.0
56	11032.0
57	10158.0
58	10042.0
59	9926.0
60	9773.0
61	9620.0
62	8556.5
63	7493.0
64	6494.5
65	5496.0
66	4332.0
67	3168.0
68	2713.5
69	2259.0
70	1778.0
71	1297.0
72	1137.5
73	978.0
74	800.0
75	500.5
76	379.0
77	313.5
78	248.0
79	157.0
80	66.0
81	65.0
82	64.0
83	40.0
84	16.0
85	9.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	213611.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.45921324276372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.22236524738493	26.679337674557956
2	9.395503455515703	7.038963349265721
3	3.5992351625279624	4.044735523919648
4	2.164540035242511	3.24327867010594
5	1.7983678468325481	3.3682722331715125
6	1.424697251833985	3.2020822897697214
7	1.29847407425922	3.4047872066513425
8	1.1909969131559544	3.5691045873105787
9	1.099766299661322	3.707674230259678
>10	6.721071772248398	37.398354953630665
>50	0.05373858055163278	1.3763336157782138
>100	0.027494157491533045	1.8318345029048129
>500	0.002499468862866641	0.6371394731544724
>1k	0.0012497344314333205	0.49810168951973444
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	1064	0.49810168951973444	No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	811	0.3796620960531059	No Hit
CTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGCT	550	0.25747737710136653	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	429	0.20083235413906586	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	341	0.15963597380284725	No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	322	0.15074130077570913	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCC	268	0.12546170375121132	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTG	227	0.10626793564001853	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2626269246433938	0.0
2	0.0	0.0	0.0	1.4254883877702926	0.0
3	0.0	0.0	0.0	1.9376342978591927	0.0
4	0.0	0.0	0.0	2.58085959992697	0.0
5	0.0	0.0	0.0	4.408480836661034	0.0
6	0.0	0.0	0.0	5.427623109296806	0.0
7	0.0	0.0	0.0	6.49077060638263	0.0
8	0.0	0.0	0.0	8.046870245446161	0.0
9	0.0	0.0	0.0	8.627832836323972	0.0
10	0.0	0.0	0.0	9.878236607665336	0.0
11	0.0	0.0	0.0	11.644999555266349	0.0
12	0.0	0.0	0.0	12.960474881911512	0.0
13	0.0	0.0	0.0	13.513349031650991	0.0
14	0.0	0.0	0.0	13.736183998015083	0.0
15	0.0	0.0	0.0	14.18091764937199	0.0
16	0.0	0.0	0.0	15.055404450145359	0.0
17	0.0	0.0	0.0	15.982323007710278	0.0
18	0.0	0.0	0.0	17.03376698765513	0.0
19	0.0	0.0	0.0	17.630178221159024	0.0
20	0.0	0.0	0.0	18.215822218893223	0.0
21	0.0	0.0	0.0	18.942376563004714	0.0
22	0.0	0.0	0.0	19.72744849282106	0.0
23	0.0	0.0	0.0	20.400634798769726	0.0
24	0.0	0.0	0.0	20.982065530333177	0.0
25	0.0	0.0	0.0	21.470336265454495	0.0
26	0.0	0.0	0.0	21.934263684922595	0.0
27	0.0	0.0	0.0	22.387423868621	0.0
28	0.0	0.0	0.0	22.87475832237104	0.0
29	0.0	0.0	0.0	23.340090163896054	0.0
30	0.0	0.0	0.0	23.84895908918548	0.0
31	4.681406856388482E-4	0.0	0.0	24.352210326247246	0.0
32	4.681406856388482E-4	0.0	0.0	24.836735935883453	0.0
33	4.681406856388482E-4	0.0	0.0	25.303472199465382	0.0
34	4.681406856388482E-4	0.0	0.0	25.754291679735594	0.0
35	4.681406856388482E-4	0.0	0.0	26.268778293252687	0.0
36	4.681406856388482E-4	0.0	0.0	26.758453450430924	0.0
37	4.681406856388482E-4	0.0	0.0	27.225189714012856	0.0
38	4.681406856388482E-4	0.0	0.0	27.669923365369762	0.0
39	4.681406856388482E-4	0.0	0.0	28.10482606232825	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAAAGA	20	7.0203794E-4	45.000004	26
GGCGTAG	20	7.0203794E-4	45.000004	1
CTAACGG	20	7.0203794E-4	45.000004	2
TCGTAAG	20	7.0203794E-4	45.000004	1
CGATGCA	20	7.0203794E-4	45.000004	10
GCTACGC	20	7.0203794E-4	45.000004	16
GACTGCC	20	7.0203794E-4	45.000004	22
GTCATTA	25	3.880111E-5	45.0	9
CTTAAGG	25	3.880111E-5	45.0	2
ACTGCGG	50	2.1827873E-11	45.0	2
CTCGTAC	25	3.880111E-5	45.0	29
AGTATGG	45	3.8198777E-10	45.0	2
ATCCGGG	25	3.880111E-5	45.0	3
TCTCGTA	25	3.880111E-5	45.0	28
TCGTACG	25	3.880111E-5	45.0	30
ACGGTTA	25	3.880111E-5	45.0	34
CGTAAGG	30	2.1571796E-6	44.999996	2
CGATGCG	30	2.1571796E-6	44.999996	10
GTAGGGC	70	0.0	41.785713	4
TGGCTAG	45	1.9157596E-8	40.0	1
>>END_MODULE