FastQCFastQC Report
Sat 18 Jun 2016
SRR3550641_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550641_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395610
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC11630.2939763908900179No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC11550.29195419731553807No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7940.20070271226713177No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT6000.15166451808599377No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5380.13599251788377442No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC4970.12562877581456486No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGG406.7957444E-945.00000433
ACTCGCA207.0276705E-445.00000411
CTCGACG207.0276705E-445.0000041
TTTAGCG502.1827873E-1145.01
AATGCGG351.2092278E-745.02
TAACACG253.886152E-545.01
CGTAAGG800.042.1875042
GCGTAAG556.002665E-1140.9090921
GTAGCGA451.9228537E-840.029
TTCGGTA451.9228537E-840.025
CGCACGG451.9228537E-840.02
TTACGGG850.039.7058833
CATTCGT403.45055E-739.37500439
CATACGA403.45055E-739.37500418
TGTAGCG403.45055E-739.3750041
ATACGAA403.45055E-739.37500419
GTTATAG403.45055E-739.3750041
AGGGTAC750.039.06
TTAGCGG1050.038.571432
GTCGAAG356.237906E-638.5714261