FastQCFastQC Report
Sat 18 Jun 2016
SRR3550640_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550640_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597100
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118921.9916261932674595No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTC32640.5466421035002512No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTT27370.45838218053927315No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC22300.37347178027131134No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG22150.37095963825154915No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCT18080.3027968514486686No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTC17540.2937531401775247No Hit
GCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC14450.2420030145704237No Hit
CGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTG13010.21788645118070676No Hit
CGTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCT11820.19795679115726011No Hit
CTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCT10830.1813766538268297No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCC9440.15809747111036676No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTC9140.1530731870708424No Hit
CCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC8640.1446993803383018No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGT7740.12962652821972867No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCT7580.1269469100653157No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG6080.10182548986769385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTC302.1637425E-645.0000045
AGTTACG302.1637425E-645.0000041
TCACGAC453.8380676E-1045.025
GTCGATG207.0305605E-445.01
ATATGCG406.8048394E-945.01
CCGGTTG207.0305605E-445.015
CGGTCTA453.8380676E-1045.031
ACTTTCG207.0305605E-445.011
TATGTCG253.8885468E-545.01
GCGTAAG253.8885468E-545.01
CGTTTTT53150.043.5183451
CTCACGA556.002665E-1140.9090924
TGTAGCG451.9255822E-840.01
AGGGCGC451.9255822E-840.06
TAATCGT403.4544973E-739.37521
CGGATGG356.2432464E-638.571432
GCGATCG356.2432464E-638.571439
TTAACGG356.2432464E-638.571432
GTTTTTT60800.038.116782
GCACCGA659.094947E-1238.0769279