##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550640_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	597100
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.277221570926145	33.0	31.0	34.0	30.0	34.0
2	32.452682967677106	34.0	31.0	34.0	30.0	34.0
3	32.341137162954276	34.0	31.0	34.0	30.0	34.0
4	35.9221353207168	37.0	35.0	37.0	35.0	37.0
5	36.008161112041535	37.0	35.0	37.0	35.0	37.0
6	36.05835203483504	37.0	35.0	37.0	35.0	37.0
7	36.12017752470273	37.0	36.0	37.0	35.0	37.0
8	36.03671914252219	37.0	36.0	37.0	35.0	37.0
9	37.89858985094624	39.0	38.0	39.0	35.0	39.0
10	37.67683637581644	39.0	37.0	39.0	35.0	39.0
11	37.5733344498409	39.0	37.0	39.0	35.0	39.0
12	37.552185563557195	39.0	37.0	39.0	35.0	39.0
13	37.5353726343996	39.0	37.0	39.0	35.0	39.0
14	38.82520013398091	40.0	38.0	41.0	35.0	41.0
15	38.88602244180204	40.0	38.0	41.0	35.0	41.0
16	38.95627198124267	40.0	38.0	41.0	35.0	41.0
17	38.90134148383855	40.0	38.0	41.0	35.0	41.0
18	38.72651147211522	40.0	38.0	41.0	35.0	41.0
19	38.56146039189416	40.0	37.0	41.0	35.0	41.0
20	38.34828504438118	40.0	36.0	41.0	34.0	41.0
21	38.29785128119243	40.0	36.0	41.0	34.0	41.0
22	38.27363255736058	40.0	36.0	41.0	34.0	41.0
23	38.227499581309665	40.0	36.0	41.0	34.0	41.0
24	38.1866688996818	40.0	36.0	41.0	34.0	41.0
25	38.11649137497906	40.0	36.0	41.0	34.0	41.0
26	38.11107017250042	40.0	36.0	41.0	34.0	41.0
27	38.09245017584994	40.0	36.0	41.0	34.0	41.0
28	38.01329090604589	40.0	36.0	41.0	34.0	41.0
29	37.95245017584994	40.0	36.0	41.0	34.0	41.0
30	37.8304789817451	40.0	36.0	41.0	34.0	41.0
31	37.690720147379	40.0	35.0	41.0	33.0	41.0
32	37.43015407804388	40.0	35.0	41.0	33.0	41.0
33	37.15216714118238	40.0	35.0	41.0	32.0	41.0
34	36.87545469770558	40.0	35.0	41.0	31.0	41.0
35	36.68042203985932	40.0	35.0	41.0	31.0	41.0
36	36.55890470607938	40.0	35.0	41.0	30.0	41.0
37	36.45865851616145	40.0	35.0	41.0	30.0	41.0
38	36.36951096968682	40.0	35.0	41.0	30.0	41.0
39	36.2458767375649	40.0	35.0	41.0	29.0	41.0
40	36.13777926645453	39.0	35.0	41.0	29.0	41.0
41	36.06972031485513	39.0	35.0	41.0	28.0	41.0
42	35.97343661028304	39.0	35.0	41.0	28.0	41.0
43	35.91667559872718	39.0	35.0	41.0	27.0	41.0
44	35.859666722492044	39.0	35.0	41.0	27.0	41.0
45	35.78977390721822	39.0	35.0	41.0	26.0	41.0
46	35.71485513314353	39.0	35.0	41.0	26.0	41.0
47	35.61756824652487	39.0	35.0	41.0	26.0	41.0
48	35.57692681292916	39.0	35.0	41.0	26.0	41.0
49	35.52023279182716	39.0	35.0	41.0	26.0	41.0
50	35.42893987606766	39.0	35.0	41.0	26.0	41.0
51	34.52862167141183	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	6.0
10	7.0
11	7.0
12	5.0
13	9.0
14	12.0
15	15.0
16	34.0
17	76.0
18	118.0
19	288.0
20	462.0
21	809.0
22	1276.0
23	1970.0
24	3332.0
25	5862.0
26	8615.0
27	10044.0
28	9543.0
29	8742.0
30	8966.0
31	9931.0
32	12030.0
33	15669.0
34	27498.0
35	39962.0
36	39469.0
37	54523.0
38	102698.0
39	234796.0
40	322.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.439289901189085	19.909897839557864	23.447831184056273	18.202981075196785
2	36.80773739742087	24.47847931669737	23.302461899179367	15.411321386702395
3	28.164963992631048	23.867358901356557	33.45938703734718	14.508290068665215
4	25.507787640261263	23.425556858147715	34.43493552168816	16.631719979902865
5	24.400770390219396	27.363255736057614	30.889968179534417	17.34600569418858
6	23.959135823145203	31.933679450678277	29.924803215541786	14.182381510634734
7	73.36409311673087	5.129291575950427	16.772567409144195	4.73404789817451
8	74.67375648970021	5.67610115558533	15.145201808742254	4.504940545972199
9	68.20850778764026	7.449673421537431	16.990621336459554	7.351197454362754
10	35.33193769887791	30.186568414001	22.307821135488194	12.173672751632893
11	24.105844917099315	22.50996483001172	34.70758666889968	18.676603583989284
12	22.970691676436108	20.526544967342154	36.22525540110534	20.2775079551164
13	21.691174007703903	21.77173002847094	38.08223078211355	18.454865181711604
14	18.246524870205995	26.567744096466257	35.29660023446659	19.889130798861164
15	17.17702227432591	25.39591358231452	38.83285881761849	18.594205325741083
16	20.374141684809917	26.355216881594373	34.59269804052922	18.67794339306649
17	20.56054262267627	26.257075866688993	33.142187238318535	20.040194272316196
18	20.2059956456205	26.157929994975714	33.934851783620836	19.70122257578295
19	20.66822977725674	27.037012225757827	32.101323061463745	20.193434935521687
20	21.44565399430581	27.30547646960308	32.98007033997655	18.268799196114553
21	21.427399095628875	27.087422542287726	33.65282197286887	17.83235638921454
22	20.450678278345336	24.36476302126947	33.36576787807737	21.81879082230782
23	19.490872550661532	26.164126611957794	33.56774409646625	20.77725674091442
24	20.014905375983922	25.167308658516163	33.96349020264612	20.854295762853795
25	20.48283369619829	27.351699882766706	31.82548986769385	20.33997655334115
26	20.178864511807067	26.871210852453526	31.741249371964496	21.20867526377491
27	21.129124099815776	26.04588846089432	31.420197621838884	21.404789817451014
28	19.240663205493217	25.82013063138503	32.806397588343664	22.132808574778092
29	21.299949757159606	23.94238820968012	31.94289063808407	22.814771395076203
30	21.41450343326076	24.899514319209512	31.29877742421705	22.38720482331268
31	21.5870038519511	24.875732708089096	29.79149221235974	23.74577122760007
32	21.9073856975381	26.131468765700888	30.40730195947077	21.553843577290234
33	20.609445653994307	26.50762016412661	30.080890973036347	22.80204320884274
34	20.04789817451013	26.46173170323229	31.575950427064143	21.914419695193434
35	20.410651482163793	26.719979902863845	30.79986601909228	22.069502595880085
36	21.914754647462736	28.202646122927483	28.803550494054598	21.079048735555183
37	20.623513649304975	27.587171328085745	30.867023949087258	20.92229107352202
38	20.238151063473456	28.010550996483	29.713448333612458	22.037849606431084
39	22.117903198794174	26.357561547479484	30.633897169653324	20.890638084073018
40	21.764026126277006	26.835705911907553	31.31770222743259	20.08256573438285
41	21.047060793836877	26.851281192430076	29.92949254731201	22.172165466421035
42	21.574610617986938	26.676268631719978	30.628872885613802	21.120247864679285
43	22.599062133645955	24.95327415843242	30.161781946072686	22.285881761848934
44	21.69100653156925	25.475799698542957	29.378496064310838	23.454697705576955
45	20.988276670574443	24.755484843409814	29.55250376821303	24.703734717802714
46	22.273488527884776	25.834533578964997	28.921956121252723	22.970021771897507
47	20.615809747111037	24.99129124099816	31.784960643108356	22.60793836878245
48	20.522525540110532	24.75029308323564	31.10534248869536	23.621838887958464
49	21.0095461396751	23.766203316027465	32.205158264947244	23.019092279350193
50	19.852286049237982	23.74443141852286	32.54346005694189	23.85982247529727
51	19.59403784960643	23.58181209177692	30.939206163121757	25.884943895494892
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	250.0
1	462.5
2	675.0
3	8697.5
4	16720.0
5	10625.5
6	4531.0
7	4349.5
8	4168.0
9	4274.0
10	4380.0
11	4532.0
12	4684.0
13	4613.0
14	4542.0
15	4389.5
16	4237.0
17	3998.0
18	3759.0
19	3563.0
20	3367.0
21	3235.0
22	3103.0
23	3184.5
24	3266.0
25	3792.5
26	4796.5
27	5274.0
28	6131.5
29	6989.0
30	8277.0
31	9565.0
32	10942.5
33	12320.0
34	13535.0
35	14750.0
36	16053.5
37	17357.0
38	18878.0
39	20399.0
40	23236.5
41	26074.0
42	29204.0
43	32334.0
44	36263.5
45	40193.0
46	52398.0
47	64603.0
48	63166.5
49	61730.0
50	58452.0
51	55174.0
52	47209.0
53	39244.0
54	34446.5
55	29649.0
56	26577.5
57	23506.0
58	21200.5
59	18895.0
60	17853.5
61	16812.0
62	14861.0
63	12910.0
64	11155.0
65	9400.0
66	7810.5
67	6221.0
68	5210.5
69	4200.0
70	3682.5
71	3165.0
72	2591.0
73	2017.0
74	1562.5
75	830.0
76	552.0
77	448.5
78	345.0
79	269.0
80	193.0
81	133.0
82	73.0
83	53.5
84	34.0
85	21.0
86	8.0
87	6.0
88	4.0
89	2.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	597100.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.40543456591466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.77153788776197	27.59456433287574
2	9.163890782285227	6.855586340519173
3	3.583454305628844	4.021219966474346
4	2.10688681997232	3.1523606832905053
5	1.567956454648519	2.9325046283279352
6	1.196807272647164	2.686025767500856
7	1.0768491219961844	2.819600655913365
8	0.912743228942412	2.7313245700549644
9	0.7739881316165167	2.605622617077854
>10	5.757974017853924	35.34992128882452
>50	0.05561778114634199	1.4369909916087542
>100	0.023772116132401096	1.6418439979733943
>500	0.003588243944513373	0.9863479321567121
>1k	0.004485304930641716	3.1909085425563033
>5k	0.0	0.0
>10k+	4.485304930641716E-4	1.9951776848456573
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11892	1.9916261932674595	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTC	3264	0.5466421035002512	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTT	2737	0.45838218053927315	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC	2230	0.37347178027131134	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG	2215	0.37095963825154915	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCT	1808	0.3027968514486686	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTC	1754	0.2937531401775247	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	1445	0.2420030145704237	No Hit
CGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTG	1301	0.21788645118070676	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCT	1182	0.19795679115726011	No Hit
CTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCT	1083	0.1813766538268297	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCC	944	0.15809747111036676	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTC	914	0.1530731870708424	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	864	0.1446993803383018	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGT	774	0.12962652821972867	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCT	758	0.1269469100653157	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG	608	0.10182548986769385	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.349522693016245E-4	0.0	0.0	0.1872383185396081	0.0
2	3.349522693016245E-4	0.0	0.0	0.7072517166303802	0.0
3	3.349522693016245E-4	0.0	0.0	1.1527382348015407	0.0
4	3.349522693016245E-4	0.0	0.0	1.809914587171328	0.0
5	3.349522693016245E-4	0.0	0.0	3.745603751465416	0.0
6	3.349522693016245E-4	0.0	0.0	5.363590688326913	0.0
7	3.349522693016245E-4	0.0	0.0	6.7209847596717465	0.0
8	3.349522693016245E-4	0.0	0.0	8.415005861664714	0.0
9	3.349522693016245E-4	0.0	0.0	9.275330765365934	0.0
10	3.349522693016245E-4	0.0	0.0	10.79986601909228	0.0
11	5.024284039524368E-4	0.0	0.0	12.180036844749623	0.0
12	5.024284039524368E-4	0.0	0.0	13.615809747111037	0.0
13	5.024284039524368E-4	0.0	0.0	14.136995478144364	0.0
14	5.024284039524368E-4	0.0	0.0	14.406799531066824	0.0
15	5.024284039524368E-4	0.0	0.0	14.753810082063305	0.0
16	5.024284039524368E-4	0.0	0.0	15.291408474292414	0.0
17	5.024284039524368E-4	0.0	0.0	15.973036342321219	0.0
18	5.024284039524368E-4	0.0	0.0	16.677608440797187	0.0
19	5.024284039524368E-4	0.0	0.0	17.21018254898677	0.0
20	5.024284039524368E-4	0.0	0.0	17.687657008876236	0.0
21	5.024284039524368E-4	0.0	0.0	18.24099815776252	0.0
22	5.024284039524368E-4	0.0	0.0	18.85965499916262	0.0
23	5.024284039524368E-4	0.0	0.0	19.43945737732373	0.0
24	5.024284039524368E-4	0.0	0.0	19.918941550829008	0.0
25	5.024284039524368E-4	0.0	0.0	20.330932842070006	0.0
26	5.024284039524368E-4	0.0	0.0	20.72383185396081	0.0
27	5.024284039524368E-4	0.0	0.0	21.137665382682968	0.0
28	5.024284039524368E-4	0.0	0.0	21.533076536593537	0.0
29	5.024284039524368E-4	0.0	0.0	21.97739072182214	0.0
30	5.024284039524368E-4	0.0	0.0	22.488360408641768	0.0
31	6.69904538603249E-4	0.0	0.0	22.936694021101992	0.0
32	6.69904538603249E-4	0.0	0.0	23.392396583486853	0.0
33	6.69904538603249E-4	0.0	0.0	23.81644615642271	0.0
34	6.69904538603249E-4	0.0	0.0	24.24903701222576	0.0
35	6.69904538603249E-4	0.0	0.0	24.717300284709427	0.0
36	6.69904538603249E-4	0.0	0.0	25.15307318707084	0.0
37	6.69904538603249E-4	0.0	0.0	25.592530564394576	0.0
38	6.69904538603249E-4	0.0	0.0	26.033830179199462	0.0
39	6.69904538603249E-4	0.0	0.0	26.54848434098141	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTC	30	2.1637425E-6	45.000004	5
AGTTACG	30	2.1637425E-6	45.000004	1
TCACGAC	45	3.8380676E-10	45.0	25
GTCGATG	20	7.0305605E-4	45.0	1
ATATGCG	40	6.8048394E-9	45.0	1
CCGGTTG	20	7.0305605E-4	45.0	15
CGGTCTA	45	3.8380676E-10	45.0	31
ACTTTCG	20	7.0305605E-4	45.0	11
TATGTCG	25	3.8885468E-5	45.0	1
GCGTAAG	25	3.8885468E-5	45.0	1
CGTTTTT	5315	0.0	43.518345	1
CTCACGA	55	6.002665E-11	40.90909	24
TGTAGCG	45	1.9255822E-8	40.0	1
AGGGCGC	45	1.9255822E-8	40.0	6
TAATCGT	40	3.4544973E-7	39.375	21
CGGATGG	35	6.2432464E-6	38.57143	2
GCGATCG	35	6.2432464E-6	38.57143	9
TTAACGG	35	6.2432464E-6	38.57143	2
GTTTTTT	6080	0.0	38.11678	2
GCACCGA	65	9.094947E-12	38.076927	9
>>END_MODULE