FastQCFastQC Report
Sat 18 Jun 2016
SRR3550636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647042
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75971.1741123451027908No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT11510.17788644322934216No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT8370.12935790876017322No Hit
CGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG7990.12348502879256679No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC6950.10741188361806499No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA207.030997E-445.0000046
TCACCGA207.030997E-445.00000419
CGACGTA207.030997E-445.00000436
AATTGCG207.030997E-445.0000041
GTATACG207.030997E-445.0000041
AACACGT406.8066583E-945.00000441
ATCGTAG253.88891E-545.01
TTAGGCG302.1640262E-644.9999961
CGTTTTT41150.043.4143371
TTTAGCG603.6379788E-1241.2499961
CGCACGG501.0804797E-940.52
TATTACG403.4550976E-739.3750041
TAATGCG403.4550976E-739.3750041
CGATAGA356.244054E-638.5714310
TATACGG659.094947E-1238.076922
ACACGCG1250.037.836
TCGATCA301.1393707E-437.49999617
TTAAGCG301.1393707E-437.4999961
TAAGACG301.1393707E-437.4999961
TGCGTAG601.546141E-1037.4999961