##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550636_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	647042
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.505756967862986	33.0	31.0	34.0	31.0	34.0
2	32.679827584608105	34.0	31.0	34.0	31.0	34.0
3	32.59204657502913	34.0	31.0	34.0	31.0	34.0
4	36.08505166588877	37.0	35.0	37.0	35.0	37.0
5	36.15539331295341	37.0	35.0	37.0	35.0	37.0
6	36.19331048061795	37.0	37.0	37.0	35.0	37.0
7	36.22265169803506	37.0	37.0	37.0	35.0	37.0
8	36.171604007158734	37.0	37.0	37.0	35.0	37.0
9	38.03052352088427	39.0	38.0	39.0	35.0	39.0
10	37.79395618831544	39.0	38.0	39.0	35.0	39.0
11	37.70810086516795	39.0	37.0	39.0	35.0	39.0
12	37.62978910178937	39.0	37.0	39.0	35.0	39.0
13	37.62853879655417	39.0	37.0	39.0	35.0	39.0
14	38.98520034248163	40.0	38.0	41.0	35.0	41.0
15	39.05774741052358	40.0	38.0	41.0	35.0	41.0
16	39.08655697775415	41.0	38.0	41.0	35.0	41.0
17	39.02758398991101	40.0	38.0	41.0	35.0	41.0
18	38.90067414480049	40.0	38.0	41.0	35.0	41.0
19	38.766772481539064	40.0	38.0	41.0	35.0	41.0
20	38.64680036226397	40.0	37.0	41.0	35.0	41.0
21	38.565473338670444	40.0	37.0	41.0	35.0	41.0
22	38.52827482605457	40.0	37.0	41.0	35.0	41.0
23	38.494219849716096	40.0	37.0	41.0	35.0	41.0
24	38.45554229864522	40.0	37.0	41.0	35.0	41.0
25	38.36292389056661	40.0	37.0	41.0	35.0	41.0
26	38.36396864500295	40.0	37.0	41.0	35.0	41.0
27	38.339135635708345	40.0	37.0	41.0	35.0	41.0
28	38.2584948117742	40.0	37.0	41.0	35.0	41.0
29	38.19651429118975	40.0	36.0	41.0	34.0	41.0
30	38.09059690097397	40.0	36.0	41.0	34.0	41.0
31	37.95848337511321	40.0	36.0	41.0	34.0	41.0
32	37.75577319555763	40.0	36.0	41.0	33.0	41.0
33	37.53421570779022	40.0	36.0	41.0	33.0	41.0
34	37.321976007739835	40.0	36.0	41.0	33.0	41.0
35	37.18543618497717	40.0	36.0	41.0	32.0	41.0
36	37.07334454332176	40.0	35.0	41.0	32.0	41.0
37	37.000038637368206	40.0	35.0	41.0	32.0	41.0
38	36.94419063986573	40.0	35.0	41.0	31.0	41.0
39	36.85859341433786	40.0	35.0	41.0	31.0	41.0
40	36.76078832595102	40.0	35.0	41.0	31.0	41.0
41	36.67210783844016	40.0	35.0	41.0	31.0	41.0
42	36.60323904785161	40.0	35.0	41.0	31.0	41.0
43	36.53953684613982	40.0	35.0	41.0	31.0	41.0
44	36.45700742764767	40.0	35.0	41.0	30.0	41.0
45	36.36396091752931	39.0	35.0	41.0	30.0	41.0
46	36.29285115958469	39.0	35.0	41.0	30.0	41.0
47	36.23563385375292	39.0	35.0	41.0	30.0	41.0
48	36.17488354697222	39.0	35.0	41.0	30.0	41.0
49	36.11465252642023	39.0	35.0	41.0	29.0	41.0
50	36.025418751796636	39.0	35.0	41.0	29.0	41.0
51	35.17688341715066	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	11.0
10	13.0
11	18.0
12	8.0
13	9.0
14	12.0
15	23.0
16	43.0
17	63.0
18	102.0
19	237.0
20	384.0
21	636.0
22	955.0
23	1511.0
24	2367.0
25	4320.0
26	6876.0
27	8917.0
28	8978.0
29	8556.0
30	8724.0
31	9484.0
32	11288.0
33	15159.0
34	29867.0
35	44037.0
36	38739.0
37	55900.0
38	107826.0
39	281518.0
40	455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.32517827281691	21.016100964079612	24.031052080081356	16.627668683022122
2	35.18396023751163	23.484719693621127	24.99528624107863	16.33603382778861
3	30.253986603651693	23.04935382865409	30.970632509172514	15.726027058521701
4	27.653691723257534	24.670268699713464	30.09974622976561	17.57629334726339
5	24.628076693630398	29.269815560659122	28.014255643374003	18.08785210233648
6	26.76132306712702	32.733578345764265	26.47138825609466	14.03371033101406
7	78.63415357890214	5.370903279848912	11.72103201955978	4.273911121689164
8	80.32940674639359	4.7009313151232845	11.32399442385502	3.6456675146281077
9	73.87294796937448	7.696409197548228	13.181679087292633	5.248963745784663
10	37.560312931772586	30.987942050129668	18.753960330241316	12.697784687856432
11	27.898498088223022	23.714225660776272	30.570503924011117	17.816772326989593
12	27.001338398434722	21.188578175759844	32.604838634895415	19.20524479091002
13	23.687952250394876	21.804921473412854	33.787451201003954	20.719675075188317
14	19.520371166013952	25.13128977717057	33.40540490416387	21.942934152651606
15	19.467669795778328	25.35461376541245	35.669091032730485	19.50862540607874
16	22.557731955576298	24.25978529987234	34.499924270758314	18.682558473793044
17	21.99640827025139	24.610767152673244	32.244892912670274	21.147931664405093
18	22.25744232986421	25.715023136056082	31.50691917989868	20.520615354181025
19	23.69011594301452	27.027611808816122	29.759891939008597	19.522380309160766
20	25.79940714822222	25.40716058617524	29.812902408189885	18.980529857412655
21	24.383888526556234	25.596174591448467	30.37453519246046	19.645401689534836
22	23.490128925170236	24.18776524553275	30.559221812494396	21.76288401680262
23	22.949360319731948	25.027123432481975	31.11637266205285	20.90714358573323
24	22.315089283230456	24.000142185515006	32.32247674803182	21.362291783222727
25	22.452792863523545	25.639293894368524	29.959878956852876	21.94803428525505
26	21.60199801558477	27.342119986028727	29.76344657688373	21.292435421502777
27	21.879259769844925	26.289947174990186	30.70125277802677	21.129540277138116
28	21.041292528151185	27.287100373700625	30.60373206066994	21.06787503747825
29	22.427910398397632	25.095897947892098	30.29865140130007	22.1775402524102
30	22.503330541139523	25.169927145378505	29.948751394808994	22.377990918672978
31	22.624651877312445	26.76966873865993	28.721010382633583	21.884669001394037
32	23.86985697991784	27.236562696084643	27.998800696090825	20.89477962790669
33	23.284732675776844	27.294518748396552	27.96974539519846	21.45100318062815
34	22.312307392719482	27.067331023333878	29.10012023948986	21.520241344456775
35	22.545213448276925	26.15688007888205	28.662745231376014	22.635161241465003
36	22.120047848516787	29.03891864824849	27.29204595683124	21.548987546403477
37	23.321515450310798	29.02640014094912	28.015028390738163	19.63705601800192
38	22.84225753505955	27.155424222847973	27.753994331125337	22.24832391096714
39	23.334497606028666	26.158425573610366	28.237579631615876	22.26949718874509
40	23.201894158339027	25.655985237434354	30.281496409815745	20.860624194410875
41	21.050101848102596	27.23826274028579	29.645525329113102	22.066110082498508
42	21.9467978894724	27.689392651481665	28.41175688749726	21.952052571548677
43	22.405964373255525	27.16376989438089	28.543123939404243	21.887141792959344
44	22.521103730515176	26.782496344904967	27.958154184736077	22.738245739843784
45	21.76056577471014	25.39989676095215	28.590106979145098	24.249430485192615
46	21.796266702934275	26.623928585779595	28.040683603228228	23.539121108057902
47	21.98218971875087	26.396895410189757	29.353890473879595	22.26702439717978
48	21.873695988822984	24.932693704581773	29.754173608513824	23.439436698081423
49	21.45533056586744	25.820580426000166	29.418028505104772	23.306060503027624
50	20.15773319197208	25.472225914237406	30.508838684351247	23.861202209439263
51	20.144751036254217	25.19929154521654	29.545068171772463	25.11088924675678
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	214.0
1	499.0
2	784.0
3	7154.0
4	13524.0
5	8937.0
6	4350.0
7	4195.5
8	4041.0
9	3907.0
10	3773.0
11	3667.5
12	3562.0
13	3352.5
14	3143.0
15	2956.5
16	2770.0
17	2650.5
18	2531.0
19	2449.5
20	2368.0
21	2405.0
22	2442.0
23	2902.0
24	3362.0
25	3758.5
26	5060.5
27	5966.0
28	7352.0
29	8738.0
30	10096.5
31	11455.0
32	13153.5
33	14852.0
34	16885.0
35	18918.0
36	20138.5
37	21359.0
38	23182.5
39	25006.0
40	27113.0
41	29220.0
42	31622.0
43	34024.0
44	36397.0
45	38770.0
46	43464.5
47	48159.0
48	50174.0
49	52189.0
50	50312.5
51	48436.0
52	44396.5
53	40357.0
54	37252.0
55	34147.0
56	32631.0
57	31115.0
58	31048.0
59	30981.0
60	30851.5
61	30722.0
62	27693.5
63	24665.0
64	20772.0
65	16879.0
66	14064.0
67	11249.0
68	8966.5
69	6684.0
70	5582.5
71	4481.0
72	3896.0
73	3311.0
74	2619.0
75	1604.5
76	1282.0
77	918.5
78	555.0
79	428.0
80	301.0
81	238.5
82	176.0
83	119.0
84	62.0
85	39.0
86	16.0
87	14.0
88	12.0
89	9.0
90	6.0
91	4.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	647042.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.02564406234577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.98305473978559	22.782098121694734
2	8.259079774991644	5.455228578627856
3	3.777552420293749	3.7426830497842247
4	2.578003568338196	3.4056091295757818
5	2.1031514032743712	3.47289648268812
6	1.8440605973939483	3.6540773351357547
7	1.5735707015064506	3.637773012642144
8	1.427954050579032	3.7727281769446397
9	1.2353684940406564	3.671895615302055
>10	8.155341930799244	42.21553510591586
>50	0.04034447339183308	0.9029348984331192
>100	0.018295749554284865	1.2276692060792076
>500	0.0032838524841024117	0.7035393569921355
>1k	4.6912178344320167E-4	0.17832499447213124
>5k	4.6912178344320167E-4	1.1770069357122337
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7597	1.1741123451027908	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT	1151	0.17788644322934216	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT	837	0.12935790876017322	No Hit
CGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG	799	0.12348502879256679	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC	695	0.10741188361806499	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.636484184952445E-4	0.0	0.0	0.0885568479325917	0.0
2	4.636484184952445E-4	0.0	0.0	0.31110808881030905	0.0
3	4.636484184952445E-4	0.0	0.0	0.5673511147653475	0.0
4	4.636484184952445E-4	0.0	0.0	0.8449219679711673	0.0
5	4.636484184952445E-4	0.0	0.0	1.5825866017971013	0.0
6	6.181978913269927E-4	0.0	0.0	2.4421907696872847	0.0
7	6.181978913269927E-4	0.0	0.0	3.14461812370758	0.0
8	6.181978913269927E-4	0.0	0.0	4.130489210901302	0.0
9	6.181978913269927E-4	0.0	0.0	4.730141165488485	0.0
10	6.181978913269927E-4	0.0	0.0	5.517725279039073	0.0
11	6.181978913269927E-4	0.0	0.0	6.314582360959567	0.0
12	7.727473641587409E-4	0.0	0.0	6.919334448150197	0.0
13	7.727473641587409E-4	0.0	0.0	7.217769480188303	0.0
14	7.727473641587409E-4	0.0	0.0	7.389319395031544	0.0
15	7.727473641587409E-4	0.0	0.0	7.585133577109368	0.0
16	7.727473641587409E-4	0.0	0.0	7.8977871606479955	0.0
17	7.727473641587409E-4	0.0	0.0	8.277978863814095	0.0
18	7.727473641587409E-4	0.0	0.0	8.686762219454069	0.0
19	7.727473641587409E-4	0.0	0.0	8.957841994800956	0.0
20	7.727473641587409E-4	0.0	0.0	9.242985772175532	0.0
21	7.727473641587409E-4	0.0	0.0	9.589949338682805	0.0
22	7.727473641587409E-4	0.0	0.0	9.981423153365624	0.0
23	9.27296836990489E-4	0.0	0.0	10.349250898705185	0.0
24	9.27296836990489E-4	0.0	0.0	10.649385974944439	0.0
25	9.27296836990489E-4	0.0	0.0	10.920929398709822	0.0
26	9.27296836990489E-4	0.0	0.0	11.18165435937698	0.0
27	9.27296836990489E-4	0.0	0.0	11.43527004429388	0.0
28	9.27296836990489E-4	0.0	0.0	11.702176983874308	0.0
29	9.27296836990489E-4	0.0	0.0	11.99149359701534	0.0
30	9.27296836990489E-4	0.0	0.0	12.327175052005897	0.0
31	9.27296836990489E-4	0.0	0.0	12.631328414538778	0.0
32	9.27296836990489E-4	0.0	0.0	12.931154391832369	0.0
33	9.27296836990489E-4	0.0	0.0	13.207488849255535	0.0
34	0.0010818463098222371	0.0	0.0	13.49448722030409	0.0
35	0.0010818463098222371	0.0	0.0	13.79307680181503	0.0
36	0.0010818463098222371	0.0	0.0	14.113612408468075	0.0
37	0.0010818463098222371	0.0	0.0	14.43770265299625	0.0
38	0.0010818463098222371	0.0	0.0	14.776320547970611	0.0
39	0.0010818463098222371	0.0	0.0	15.11478389347214	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	20	7.030997E-4	45.000004	6
TCACCGA	20	7.030997E-4	45.000004	19
CGACGTA	20	7.030997E-4	45.000004	36
AATTGCG	20	7.030997E-4	45.000004	1
GTATACG	20	7.030997E-4	45.000004	1
AACACGT	40	6.8066583E-9	45.000004	41
ATCGTAG	25	3.88891E-5	45.0	1
TTAGGCG	30	2.1640262E-6	44.999996	1
CGTTTTT	4115	0.0	43.414337	1
TTTAGCG	60	3.6379788E-12	41.249996	1
CGCACGG	50	1.0804797E-9	40.5	2
TATTACG	40	3.4550976E-7	39.375004	1
TAATGCG	40	3.4550976E-7	39.375004	1
CGATAGA	35	6.244054E-6	38.57143	10
TATACGG	65	9.094947E-12	38.07692	2
ACACGCG	125	0.0	37.8	36
TCGATCA	30	1.1393707E-4	37.499996	17
TTAAGCG	30	1.1393707E-4	37.499996	1
TAAGACG	30	1.1393707E-4	37.499996	1
TGCGTAG	60	1.546141E-10	37.499996	1
>>END_MODULE