FastQCFastQC Report
Sat 18 Jun 2016
SRR3550635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592960
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45800.7723961144090663No Hit
GCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC6230.10506610901241231No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG253.8885126E-545.0000041
TCGTTTA207.0305204E-445.038
CATTACG207.0305204E-445.02
TTACGTC207.0305204E-445.024
TAGTACG207.0305204E-445.01
CGTTTTT25400.042.3425181
CTACGAA603.6379788E-1241.24999611
GCTAACG451.9255822E-840.0000041
AATACGG451.9255822E-840.0000042
TGCGAAG403.4544428E-739.3751
TACGTAG403.4544428E-739.3751
TTAACGG700.038.5714262
AACGGGA2400.038.4374964
CTAACGG601.546141E-1037.4999962
AAATGCG301.1392555E-437.4999961
CACGACC2050.037.31707427
TAGGGTA1450.037.241385
CCCCACG552.744855E-936.8181838
ACACGAC2150.036.6279126
AGACACG2150.036.6279124