##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550635_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	592960
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.530850310307606	33.0	31.0	34.0	31.0	34.0
2	32.686526915812195	34.0	31.0	34.0	31.0	34.0
3	32.634594239071774	34.0	31.0	34.0	31.0	34.0
4	36.10560577441986	37.0	35.0	37.0	35.0	37.0
5	36.15441513761468	37.0	35.0	37.0	35.0	37.0
6	36.1962830545062	37.0	37.0	37.0	35.0	37.0
7	36.20023104425256	37.0	37.0	37.0	35.0	37.0
8	36.1314439422558	37.0	37.0	37.0	35.0	37.0
9	37.99148171883432	39.0	38.0	39.0	35.0	39.0
10	37.75530895844577	39.0	38.0	39.0	35.0	39.0
11	37.663569212088504	39.0	37.0	39.0	35.0	39.0
12	37.54190333243389	39.0	37.0	39.0	35.0	39.0
13	37.50057845385861	39.0	37.0	39.0	35.0	39.0
14	38.795687736103616	40.0	38.0	41.0	35.0	41.0
15	38.888823866702644	40.0	38.0	41.0	35.0	41.0
16	38.93566851052348	40.0	38.0	41.0	35.0	41.0
17	38.904818200215864	40.0	38.0	41.0	35.0	41.0
18	38.801165339989204	40.0	38.0	41.0	35.0	41.0
19	38.698040339989205	40.0	37.0	41.0	35.0	41.0
20	38.596686117107396	40.0	37.0	41.0	35.0	41.0
21	38.505239813815436	40.0	37.0	41.0	35.0	41.0
22	38.4825215866163	40.0	37.0	41.0	35.0	41.0
23	38.44448192120885	40.0	37.0	41.0	35.0	41.0
24	38.4071269562871	40.0	37.0	41.0	35.0	41.0
25	38.328487587695626	40.0	37.0	41.0	34.0	41.0
26	38.305747436589314	40.0	37.0	41.0	34.0	41.0
27	38.28194144630329	40.0	37.0	41.0	35.0	41.0
28	38.197581624392875	40.0	36.0	41.0	34.0	41.0
29	38.1356415272531	40.0	36.0	41.0	34.0	41.0
30	38.03462796815974	40.0	36.0	41.0	34.0	41.0
31	37.919372301672965	40.0	36.0	41.0	34.0	41.0
32	37.73374932541824	40.0	36.0	41.0	33.0	41.0
33	37.57622605234754	40.0	36.0	41.0	33.0	41.0
34	37.36696910415542	40.0	35.0	41.0	33.0	41.0
35	37.23941581219644	40.0	35.0	41.0	33.0	41.0
36	37.13486913113869	40.0	35.0	41.0	32.0	41.0
37	37.04000607123584	40.0	35.0	41.0	32.0	41.0
38	36.93897396114409	40.0	35.0	41.0	32.0	41.0
39	36.856442255801404	40.0	35.0	41.0	31.0	41.0
40	36.7293932137075	40.0	35.0	41.0	31.0	41.0
41	36.65491432811657	39.0	35.0	41.0	31.0	41.0
42	36.57883331084727	39.0	35.0	41.0	31.0	41.0
43	36.49190501888829	39.0	35.0	41.0	31.0	41.0
44	36.417795466810574	39.0	35.0	41.0	31.0	41.0
45	36.30397497301673	39.0	35.0	41.0	30.0	41.0
46	36.22447551268214	39.0	35.0	41.0	30.0	41.0
47	36.14017471667566	39.0	35.0	41.0	30.0	41.0
48	36.07893787101997	39.0	35.0	41.0	30.0	41.0
49	35.992717889908256	39.0	35.0	41.0	30.0	41.0
50	35.90987756341069	38.0	35.0	41.0	30.0	41.0
51	35.00334929843497	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	8.0
10	13.0
11	15.0
12	13.0
13	12.0
14	16.0
15	19.0
16	47.0
17	76.0
18	151.0
19	212.0
20	376.0
21	576.0
22	937.0
23	1404.0
24	2366.0
25	3874.0
26	5756.0
27	6949.0
28	7038.0
29	7064.0
30	7583.0
31	8939.0
32	10869.0
33	15397.0
34	31059.0
35	43543.0
36	37867.0
37	56451.0
38	103208.0
39	240699.0
40	419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.289530491095526	20.87358337830545	22.8600580140313	13.976828116567727
2	34.58934835402051	23.637513491635186	24.559498111171074	17.213640043173235
3	31.445797355639506	23.85000674581759	28.37948596869941	16.324709929843497
4	27.982663248785755	25.38973961144091	28.398374257960064	18.229222881813275
5	25.336279007015648	29.476693200215863	26.774993254182405	18.412034538586077
6	26.8694347004857	34.10786562331354	25.083648138154345	13.939051538046412
7	80.01315434430653	5.48097679438748	10.22176875337291	4.284100107933082
8	81.39975715056666	5.161225040474905	9.81398407987048	3.6250337290879653
9	75.08735833783055	7.259005666486779	11.984282245008094	5.669353750674581
10	40.95284673502428	25.73377630868861	18.794859686994066	14.518517269293039
11	31.350512682137076	25.12732730706962	25.401713437668644	18.120446573124664
12	27.83121964382083	23.740049919050186	28.619805720453318	19.808924716675662
13	24.93625202374528	23.688444414463035	30.812702374527795	20.562601187263894
14	21.724230976794388	25.615049919050186	31.6724568267674	20.98826227738802
15	21.155221262817054	24.833041014570966	33.34508229897464	20.666655423637344
16	24.92798839719374	24.72375876956287	31.01339044792229	19.3348623853211
17	25.122605234754452	24.336548839719374	29.377698327037237	21.163147598488937
18	25.683182676740422	24.565232056125204	29.115960604425258	20.63562466270912
19	25.210638154344306	25.900229357798167	27.940164597949273	20.948967889908257
20	27.37857528332434	25.62145844576363	28.134781435509982	18.86518483540205
21	25.992647058823533	25.78352671343767	28.846127900701564	19.377698327037237
22	25.25043847814355	23.551841608202913	29.41007825148408	21.787641662169456
23	24.084592552617377	25.48401241230437	29.54246492174852	20.888930113329735
24	23.27087830545062	24.639604695089044	30.15211818672423	21.937398812736102
25	23.93719643820831	26.03700080949811	28.0261737722612	21.99962898003238
26	22.826666216945494	27.068773610361575	28.21893550998381	21.885624662709123
27	23.288586076632487	26.127057474365895	28.982730706961686	21.601625742039936
28	21.550694819212087	27.383297355639503	29.111913113869402	21.95409471127901
29	23.199035348084188	26.586447652455476	28.883735833783053	21.33078116567728
30	24.211413923367513	25.492613329735565	28.79975040474906	21.49622234214787
31	24.44869805720453	26.60516729627631	27.216507015650297	21.729627630868862
32	24.547018348623855	26.85729222881813	27.446876686454395	21.14881273610362
33	24.698799244468432	26.614780086346467	26.808722342147867	21.877698327037237
34	22.48482191041554	27.03706826767404	28.828588774959524	21.64952104695089
35	22.990589584457634	27.896822719913654	27.40876281705343	21.703824878575283
36	24.19151376146789	28.722679438747978	26.269056934700487	20.816749865083647
37	24.713977334052885	27.834592552617377	26.005464112250404	21.44596600107933
38	23.574102806260118	27.894967620075555	26.664867781975175	21.866061791689155
39	23.937027792768482	25.718935509983808	27.24635725849973	23.097679438747974
40	24.536393685914735	25.079769293038318	28.779512951969778	21.604324069077173
41	22.916722881813275	26.06735698866703	27.708108472746893	23.3078116567728
42	23.834997301672963	26.24190501888829	27.620075553157044	22.303022126281704
43	23.754047490555855	25.814220183486235	27.856179168915276	22.575553157042634
44	23.217249055585537	25.99652590393956	27.373178629249868	23.413046411225043
45	22.99092687533729	25.453318942255805	27.17805585536967	24.377698327037237
46	22.636940097139775	26.198225849973017	27.45126146788991	23.7135725849973
47	22.276882083108475	26.71647328656233	28.707669994603346	22.29897463572585
48	22.745885051268214	26.154883971937398	28.43311521856449	22.666115758229896
49	23.22399487317863	25.437297625472205	28.9272463572585	22.411461144090662
50	21.533492984349703	26.12064894765245	28.834997301672967	23.51086076632488
51	21.655254991905018	25.495817593092283	27.969340259039395	24.879587155963304
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	193.0
1	596.5
2	1000.0
3	4532.5
4	8065.0
5	5409.5
6	2754.0
7	2698.5
8	2643.0
9	2585.5
10	2528.0
11	2499.5
12	2471.0
13	2432.5
14	2394.0
15	2276.5
16	2159.0
17	2159.0
18	2159.0
19	2000.5
20	1842.0
21	1911.5
22	1981.0
23	2430.0
24	2879.0
25	3170.5
26	4168.5
27	4875.0
28	5578.0
29	6281.0
30	7284.0
31	8287.0
32	9505.0
33	10723.0
34	12440.5
35	14158.0
36	15686.0
37	17214.0
38	19378.5
39	21543.0
40	23651.0
41	25759.0
42	28186.0
43	30613.0
44	32843.0
45	35073.0
46	39544.5
47	44016.0
48	45552.0
49	47088.0
50	47697.5
51	48307.0
52	44975.0
53	41643.0
54	38945.0
55	36247.0
56	34400.5
57	32554.0
58	31101.0
59	29648.0
60	28390.5
61	27133.0
62	24858.5
63	22584.0
64	19411.5
65	16239.0
66	14295.5
67	12352.0
68	10629.0
69	8906.0
70	7295.0
71	5684.0
72	4810.0
73	3936.0
74	3202.0
75	2051.5
76	1635.0
77	1229.0
78	823.0
79	576.5
80	330.0
81	277.0
82	224.0
83	138.5
84	53.0
85	34.0
86	15.0
87	14.0
88	13.0
89	8.5
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	592960.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.22027298451461
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.38010437326476	22.354459026007063
2	8.074116292190455	5.203004620861867
3	3.542846728734419	3.4245446622635267
4	2.3142021996678674	2.9825690645865146
5	1.8746396087553747	3.020069997084091
6	1.5824294415345501	3.059178515098573
7	1.4650913178560727	3.3043949545995446
8	1.2882032975628765	3.320500952562223
9	1.2771859646663017	3.7036152390184984
>10	9.134387043195057	46.15479765946183
>50	0.041022922367273056	0.8999896385890287
>100	0.023141135694287027	1.407006960871181
>500	0.002103739608571548	0.3897526207399393
>1k	5.25934902142887E-4	0.776116088256053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4580	0.7723961144090663	No Hit
GCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	623	0.10506610901241231	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6864543982730707E-4	0.0	0.0	0.09713977334052887	0.0
2	1.6864543982730707E-4	0.0	0.0	0.33611036157582297	0.0
3	1.6864543982730707E-4	0.0	0.0	0.5337628170534269	0.0
4	1.6864543982730707E-4	0.0	0.0	0.7467620075553157	0.0
5	1.6864543982730707E-4	0.0	0.0	1.3896384241770103	0.0
6	1.6864543982730707E-4	0.0	0.0	1.9761872638963842	0.0
7	1.6864543982730707E-4	0.0	0.0	2.4558148947652456	0.0
8	1.6864543982730707E-4	0.0	0.0	3.1383229897463574	0.0
9	1.6864543982730707E-4	0.0	0.0	3.4987182946573125	0.0
10	1.6864543982730707E-4	0.0	0.0	4.136198057204533	0.0
11	1.6864543982730707E-4	0.0	0.0	4.886332973556395	0.0
12	1.6864543982730707E-4	0.0	0.0	5.513356718834323	0.0
13	1.6864543982730707E-4	0.0	0.0	5.794151376146789	0.0
14	1.6864543982730707E-4	0.0	0.0	5.942728008634647	0.0
15	1.6864543982730707E-4	0.0	0.0	6.159268753372909	0.0
16	1.6864543982730707E-4	0.0	0.0	6.516459794927145	0.0
17	3.3729087965461414E-4	0.0	0.0	6.907885860766325	0.0
18	3.3729087965461414E-4	0.0	0.0	7.329499460334593	0.0
19	5.059363194819212E-4	0.0	0.0	7.609113599568268	0.0
20	5.059363194819212E-4	0.0	0.0	7.896485429033999	0.0
21	5.059363194819212E-4	0.0	0.0	8.255025634106854	0.0
22	5.059363194819212E-4	0.0	0.0	8.631273610361577	0.0
23	6.745817593092283E-4	0.0	0.0	8.991500269832704	0.0
24	6.745817593092283E-4	0.0	0.0	9.305180787911494	0.0
25	6.745817593092283E-4	0.0	0.0	9.601828116567727	0.0
26	8.432271991365354E-4	0.0	0.0	9.864915002698327	0.0
27	8.432271991365354E-4	0.0	0.0	10.135759579060982	0.0
28	8.432271991365354E-4	0.0	0.0	10.422288181327577	0.0
29	8.432271991365354E-4	0.0	0.0	10.734113599568268	0.0
30	8.432271991365354E-4	0.0	0.0	11.109686994063681	0.0
31	8.432271991365354E-4	0.0	0.0	11.426065839179708	0.0
32	8.432271991365354E-4	0.0	0.0	11.7397463572585	0.0
33	8.432271991365354E-4	0.0	0.0	12.064894765245548	0.0
34	8.432271991365354E-4	0.0	0.0	12.386838909875877	0.0
35	0.0010118726389638424	0.0	0.0	12.710806799784134	0.0
36	0.0010118726389638424	0.0	0.0	13.024655963302752	0.0
37	0.0011805180787911496	0.0	0.0	13.365994333513221	0.0
38	0.0011805180787911496	0.0	0.0	13.74055585536967	0.0
39	0.0011805180787911496	0.0	0.0	14.214280895844576	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACG	25	3.8885126E-5	45.000004	1
TCGTTTA	20	7.0305204E-4	45.0	38
CATTACG	20	7.0305204E-4	45.0	2
TTACGTC	20	7.0305204E-4	45.0	24
TAGTACG	20	7.0305204E-4	45.0	1
CGTTTTT	2540	0.0	42.342518	1
CTACGAA	60	3.6379788E-12	41.249996	11
GCTAACG	45	1.9255822E-8	40.000004	1
AATACGG	45	1.9255822E-8	40.000004	2
TGCGAAG	40	3.4544428E-7	39.375	1
TACGTAG	40	3.4544428E-7	39.375	1
TTAACGG	70	0.0	38.571426	2
AACGGGA	240	0.0	38.437496	4
CTAACGG	60	1.546141E-10	37.499996	2
AAATGCG	30	1.1392555E-4	37.499996	1
CACGACC	205	0.0	37.317074	27
TAGGGTA	145	0.0	37.24138	5
CCCCACG	55	2.744855E-9	36.81818	38
ACACGAC	215	0.0	36.62791	26
AGACACG	215	0.0	36.62791	24
>>END_MODULE