FastQCFastQC Report
Sat 18 Jun 2016
SRR3550634_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550634_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511062
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20400.3991687896967491No Hit
GCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC10440.2042804982565716RNA PCR Primer, Index 47 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC9640.18862682022924812RNA PCR Primer, Index 17 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGCT8780.17179911634987535RNA PCR Primer, Index 17 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC8180.16005885782938273RNA PCR Primer, Index 17 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCG7770.15203634784037945No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGC7320.14323115395001No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGT253.8877537E-545.00000444
TCTACGG351.2101555E-745.0000042
TATACGG351.2101555E-745.0000042
TACGGGT207.0296053E-445.04
ACGTGCG207.0296053E-445.01
TCTCGCG302.1631258E-644.99999632
AGTACGG556.002665E-1140.9090922
CGGGTAC501.0786607E-940.5000046
CGTTTTT11850.039.4936681
CGAGAGG750.039.02
ATAGTAG356.24148E-638.571431
TACCACG301.1390349E-437.49999623
ACGCTAC301.1390349E-437.49999630
GGCCTAT301.1390349E-437.4999968
TAGTACG301.1390349E-437.4999961
CGTATGG850.037.0588232
TTAGCGG1100.036.818182
GCGTTAG552.743036E-936.818181
TACGGGA2650.036.5094344
AACGGGC1050.036.428574