Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550634_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 511062 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2040 | 0.3991687896967491 | No Hit |
GCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC | 1044 | 0.2042804982565716 | RNA PCR Primer, Index 47 (95% over 23bp) |
CCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC | 964 | 0.18862682022924812 | RNA PCR Primer, Index 17 (95% over 24bp) |
CTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGCT | 878 | 0.17179911634987535 | RNA PCR Primer, Index 17 (96% over 25bp) |
GAATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC | 818 | 0.16005885782938273 | RNA PCR Primer, Index 17 (95% over 21bp) |
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCG | 777 | 0.15203634784037945 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGC | 732 | 0.14323115395001 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TAGCCGT | 25 | 3.8877537E-5 | 45.000004 | 44 |
TCTACGG | 35 | 1.2101555E-7 | 45.000004 | 2 |
TATACGG | 35 | 1.2101555E-7 | 45.000004 | 2 |
TACGGGT | 20 | 7.0296053E-4 | 45.0 | 4 |
ACGTGCG | 20 | 7.0296053E-4 | 45.0 | 1 |
TCTCGCG | 30 | 2.1631258E-6 | 44.999996 | 32 |
AGTACGG | 55 | 6.002665E-11 | 40.909092 | 2 |
CGGGTAC | 50 | 1.0786607E-9 | 40.500004 | 6 |
CGTTTTT | 1185 | 0.0 | 39.493668 | 1 |
CGAGAGG | 75 | 0.0 | 39.0 | 2 |
ATAGTAG | 35 | 6.24148E-6 | 38.57143 | 1 |
TACCACG | 30 | 1.1390349E-4 | 37.499996 | 23 |
ACGCTAC | 30 | 1.1390349E-4 | 37.499996 | 30 |
GGCCTAT | 30 | 1.1390349E-4 | 37.499996 | 8 |
TAGTACG | 30 | 1.1390349E-4 | 37.499996 | 1 |
CGTATGG | 85 | 0.0 | 37.058823 | 2 |
TTAGCGG | 110 | 0.0 | 36.81818 | 2 |
GCGTTAG | 55 | 2.743036E-9 | 36.81818 | 1 |
TACGGGA | 265 | 0.0 | 36.509434 | 4 |
AACGGGC | 105 | 0.0 | 36.42857 | 4 |