##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550634_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	511062
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.452003866458476	33.0	31.0	34.0	31.0	34.0
2	32.59442494257057	34.0	31.0	34.0	31.0	34.0
3	32.56875878073502	34.0	31.0	34.0	31.0	34.0
4	36.03539296601978	37.0	35.0	37.0	35.0	37.0
5	36.11147962478134	37.0	35.0	37.0	35.0	37.0
6	36.154286564056804	37.0	36.0	37.0	35.0	37.0
7	36.210995926130295	37.0	37.0	37.0	35.0	37.0
8	36.16021735131941	37.0	37.0	37.0	35.0	37.0
9	38.01734623196403	39.0	39.0	39.0	35.0	39.0
10	37.74978378357225	39.0	38.0	39.0	35.0	39.0
11	37.650817317664	39.0	37.0	39.0	35.0	39.0
12	37.58642786980836	39.0	37.0	39.0	35.0	39.0
13	37.59537003338147	39.0	37.0	39.0	35.0	39.0
14	38.93265396370695	40.0	38.0	41.0	35.0	41.0
15	39.012851669660435	40.0	38.0	41.0	35.0	41.0
16	39.048788600991664	40.0	38.0	41.0	35.0	41.0
17	38.997900450434585	40.0	38.0	41.0	35.0	41.0
18	38.94410462918393	40.0	38.0	41.0	35.0	41.0
19	38.89730208859199	40.0	38.0	41.0	35.0	41.0
20	38.82791716073588	40.0	38.0	41.0	35.0	41.0
21	38.76394644876747	40.0	38.0	41.0	35.0	41.0
22	38.728385205708896	40.0	38.0	41.0	35.0	41.0
23	38.68502451757321	40.0	38.0	41.0	35.0	41.0
24	38.6729868391702	40.0	38.0	41.0	35.0	41.0
25	38.61472776297201	40.0	38.0	41.0	35.0	41.0
26	38.62011849834267	40.0	38.0	41.0	35.0	41.0
27	38.59289088212389	40.0	38.0	41.0	35.0	41.0
28	38.53092775436249	40.0	38.0	41.0	35.0	41.0
29	38.49274060681483	40.0	38.0	41.0	35.0	41.0
30	38.42283715087406	40.0	38.0	41.0	35.0	41.0
31	38.3529630455796	40.0	38.0	41.0	34.0	41.0
32	38.235527196308865	40.0	37.0	41.0	34.0	41.0
33	38.158687204292235	40.0	37.0	41.0	34.0	41.0
34	38.051482990322114	40.0	37.0	41.0	34.0	41.0
35	37.97730999369939	40.0	37.0	41.0	34.0	41.0
36	37.91564819924002	40.0	37.0	41.0	34.0	41.0
37	37.85155030113763	40.0	37.0	41.0	34.0	41.0
38	37.80141352712587	40.0	37.0	41.0	33.0	41.0
39	37.74408388806055	40.0	37.0	41.0	33.0	41.0
40	37.64872950835711	40.0	36.0	41.0	33.0	41.0
41	37.59795093354622	40.0	36.0	41.0	33.0	41.0
42	37.557243543836165	40.0	36.0	41.0	33.0	41.0
43	37.49403594867159	40.0	36.0	41.0	33.0	41.0
44	37.42039909052131	40.0	36.0	41.0	33.0	41.0
45	37.30451295537528	40.0	35.0	41.0	33.0	41.0
46	37.248533446039815	40.0	35.0	41.0	33.0	41.0
47	37.171599531955025	40.0	35.0	41.0	33.0	41.0
48	37.130471449647985	39.0	35.0	41.0	32.0	41.0
49	37.07135337786805	39.0	35.0	41.0	32.0	41.0
50	36.97184881677761	39.0	35.0	41.0	32.0	41.0
51	36.04501215116757	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	9.0
10	5.0
11	12.0
12	5.0
13	6.0
14	8.0
15	8.0
16	25.0
17	33.0
18	75.0
19	113.0
20	230.0
21	414.0
22	639.0
23	912.0
24	1267.0
25	2079.0
26	2983.0
27	3723.0
28	4109.0
29	4641.0
30	5290.0
31	6639.0
32	8428.0
33	11568.0
34	22627.0
35	34232.0
36	29085.0
37	45193.0
38	89673.0
39	236696.0
40	329.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.196101451487294	21.584856631876367	26.020326300918477	12.198715615717857
2	31.40460452939174	24.525400049309084	26.547855250439284	17.522140170859895
3	31.223217535250125	24.289616524022524	28.13513820240988	16.352027738317464
4	28.28776156317629	25.462272679244396	27.99308107431192	18.25688468326739
5	26.27137216228168	29.877979579777016	25.124348904829553	18.726299353111756
6	26.072766122310014	34.48700940394708	24.183367184412067	15.256857289330844
7	81.93115512403583	5.168257471696194	8.360238092442795	4.54034931182518
8	83.67712723700843	4.850096465790844	7.414755939592456	4.0580203576082745
9	77.40274174170648	7.4341273661512695	9.510979098426414	5.652151793715831
10	37.191769296093234	35.01101627591173	15.408893637171223	12.388320790823814
11	27.29179629868783	24.252830380658317	30.021015062751683	18.434358257902172
12	26.19134273336699	22.769840058544755	31.01091452700455	20.027902681083702
13	23.804939518101524	23.761304890600357	32.25009881384255	20.18365677745557
14	19.936915677549884	27.73166465125562	30.569872148584707	21.76154752260978
15	19.013740015888484	26.574270832110393	34.53886221241259	19.87312693958854
16	22.115711987977978	25.883160947204058	32.08632220748168	19.91480485733629
17	21.806356175962996	26.154165248052095	29.291357995703066	22.748120580281846
18	22.332515428656404	26.40677647721803	30.203576082745343	21.057132011380226
19	23.622378498107864	27.029988533680843	28.13376850558249	21.213864462628802
20	25.801174808535947	26.28643882738298	28.501825610199937	19.410560753881136
21	24.142080608615004	26.868364308048733	28.928584007419843	20.060971075916427
22	22.98331709264238	24.94530996239204	29.274725962799035	22.796646982166546
23	22.149758737687403	26.96600412474416	29.0164402753482	21.86779686222024
24	21.72084795973874	25.281864039979496	30.50119163623983	22.496096364041936
25	21.57663845091202	27.232703664134682	28.694365849936016	22.49629203501728
26	21.63338303376107	27.995820467966702	28.380118263537497	21.99067823473473
27	22.675917990380814	27.281817078945416	28.940519936915678	21.101744993758096
28	20.746602173513192	27.68079019766682	30.01807999812156	21.554527630698427
29	23.188184603824975	25.3280423901601	29.60384454332351	21.879928462691414
30	23.10893785881165	25.48164410580321	29.894611612681043	21.514806422704094
31	23.9291905874434	27.084189393850455	27.16539284861719	21.821227170088953
32	24.632627743796252	27.658875048428566	27.445789356281626	20.262707851493555
33	24.105881478176816	27.002790268108374	27.360868152983397	21.530460100731418
34	23.387181985747326	27.267924439696163	28.626859363443184	20.718034211113327
35	22.984882460445114	26.212083856753193	28.6288160731966	22.174217609605094
36	24.75785716801484	27.45498589212268	27.557321812226306	20.22983512763618
37	23.531391494574045	28.152748590190622	28.595160665437852	19.72069924979748
38	23.271736110295816	27.03820671464519	27.901115715901398	21.7889414591576
39	24.212130817787276	26.726111508975425	27.69585686276812	21.365900810469178
40	24.010589713185485	25.409245844926836	29.973467015743687	20.60669742614399
41	21.974828885732066	26.66564917759489	28.89884201916793	22.460679917505118
42	23.85561830071498	25.501015532362022	29.218177050925327	21.425189115997668
43	24.535183598076163	25.46266402119508	28.529023875772413	21.473128504956346
44	23.50047548047008	25.99841115168023	28.12692002144554	22.37419334640415
45	22.565755231263527	25.388504721540635	28.382661986216934	23.663078060978904
46	23.50556292582896	26.181950526550597	27.90796420003835	22.40452234758209
47	21.997526718871683	26.105834517142736	29.969749267212197	21.926889496773384
48	22.763382916358484	24.69817752053567	29.630847137920647	22.907592425185204
49	23.271736110295816	24.59153683897453	29.694635875881985	22.44209117484767
50	21.76937436162344	25.420399090521308	29.026615166065955	23.783611381789292
51	21.16044628636056	24.901871005866216	28.575397896928358	25.36228481084487
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	83.0
1	334.5
2	586.0
3	2155.5
4	3725.0
5	2472.5
6	1220.0
7	1164.5
8	1109.0
9	1115.0
10	1121.0
11	1104.0
12	1087.0
13	1021.0
14	955.0
15	940.0
16	925.0
17	972.0
18	1019.0
19	1025.5
20	1032.0
21	1327.0
22	1622.0
23	2010.5
24	2399.0
25	2922.0
26	4066.5
27	4688.0
28	5552.0
29	6416.0
30	8070.5
31	9725.0
32	11430.5
33	13136.0
34	14400.5
35	15665.0
36	17205.5
37	18746.0
38	20683.0
39	22620.0
40	24271.5
41	25923.0
42	28029.5
43	30136.0
44	32473.0
45	34810.0
46	40187.0
47	45564.0
48	46336.5
49	47109.0
50	46516.0
51	45923.0
52	40301.0
53	34679.0
54	31417.5
55	28156.0
56	26427.0
57	24698.0
58	22872.5
59	21047.0
60	20309.5
61	19572.0
62	17515.0
63	15458.0
64	12900.5
65	10343.0
66	8297.5
67	6252.0
68	5035.5
69	3819.0
70	3168.5
71	2518.0
72	1987.0
73	1456.0
74	1276.5
75	840.5
76	584.0
77	461.0
78	338.0
79	221.0
80	104.0
81	101.5
82	99.0
83	65.0
84	31.0
85	21.5
86	12.0
87	8.5
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	511062.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.030947994347564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.48608797858739	22.577362183671394
2	9.155899008064283	5.865442499376109
3	3.4629968553553168	3.327692165354259
4	1.9934148925907031	2.5540387502292297
5	1.490267309211203	2.3867337339510164
6	1.288925609593716	2.477130550168743
7	1.0950480682428572	2.4552799407638406
8	1.0232962987695005	2.6221720422955372
9	0.9510436385009936	2.741654639266237
>10	8.976340865112615	49.24760383920164
>50	0.050301736237245175	1.093659469213941
>100	0.022083689079766176	1.2260932604913746
>500	0.0030671790388564135	0.819163910735343
>1k	0.0012268716155425653	0.605973015281314
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2040	0.3991687896967491	No Hit
GCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	1044	0.2042804982565716	RNA PCR Primer, Index 47 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	964	0.18862682022924812	RNA PCR Primer, Index 17 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGCT	878	0.17179911634987535	RNA PCR Primer, Index 17 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC	818	0.16005885782938273	RNA PCR Primer, Index 17 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCG	777	0.15203634784037945	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGC	732	0.14323115395001	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.913419506830874E-4	0.0	0.0	0.1768865617087555	0.0
2	3.913419506830874E-4	0.0	0.0	0.7183081504788069	0.0
3	3.913419506830874E-4	0.0	0.0	1.0401869049156462	0.0
4	3.913419506830874E-4	0.0	0.0	1.4252673843878043	0.0
5	3.913419506830874E-4	0.0	0.0	2.504784155347101	0.0
6	3.913419506830874E-4	0.0	0.0	3.2107650343793903	0.0
7	3.913419506830874E-4	0.0	0.0	3.897178815877526	0.0
8	3.913419506830874E-4	0.0	0.0	5.025221988721525	0.0
9	3.913419506830874E-4	0.0	0.0	5.507746613913771	0.0
10	3.913419506830874E-4	0.0	0.0	6.537954299087	0.0
11	3.913419506830874E-4	0.0	0.0	7.642516954890013	0.0
12	3.913419506830874E-4	0.0	0.0	8.705010350994595	0.0
13	3.913419506830874E-4	0.0	0.0	9.112788663606372	0.0
14	3.913419506830874E-4	0.0	0.0	9.29006656726581	0.0
15	3.913419506830874E-4	0.0	0.0	9.594530604897253	0.0
16	3.913419506830874E-4	0.0	0.0	10.111493321749611	0.0
17	3.913419506830874E-4	0.0	0.0	10.786558186677937	0.0
18	3.913419506830874E-4	0.0	0.0	11.471210929397998	0.0
19	3.913419506830874E-4	0.0	0.0	11.944930360699876	0.0
20	3.913419506830874E-4	0.0	0.0	12.392234210330646	0.0
21	3.913419506830874E-4	0.0	0.0	12.937373547632186	0.0
22	3.913419506830874E-4	0.0	0.0	13.54258387436358	0.0
23	3.913419506830874E-4	0.0	0.0	14.098680786284246	0.0
24	5.87012926024631E-4	0.0	0.0	14.52993961593701	0.0
25	5.87012926024631E-4	0.0	0.0	14.918346501989975	0.0
26	5.87012926024631E-4	0.0	0.0	15.285816593681393	0.0
27	5.87012926024631E-4	0.0	0.0	15.665222614868647	0.0
28	7.826839013661748E-4	0.0	0.0	16.05891261725583	0.0
29	7.826839013661748E-4	0.0	0.0	16.49095413080996	0.0
30	7.826839013661748E-4	0.0	0.0	16.992067498659654	0.0
31	7.826839013661748E-4	0.0	0.0	17.43506658683291	0.0
32	9.783548767077184E-4	0.0	0.0	17.859672603324057	0.0
33	9.783548767077184E-4	0.0	0.0	18.253949618637268	0.0
34	0.0013696968273908057	0.0	0.0	18.6582058536929	0.0
35	0.0013696968273908057	0.0	0.0	19.08926901237032	0.0
36	0.0013696968273908057	0.0	0.0	19.532268100543575	0.0
37	0.0013696968273908057	0.0	0.0	19.96626632385112	0.0
38	0.0013696968273908057	0.0	0.0	20.39263337912034	0.0
39	0.0013696968273908057	0.0	0.0	20.811760608301928	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCCGT	25	3.8877537E-5	45.000004	44
TCTACGG	35	1.2101555E-7	45.000004	2
TATACGG	35	1.2101555E-7	45.000004	2
TACGGGT	20	7.0296053E-4	45.0	4
ACGTGCG	20	7.0296053E-4	45.0	1
TCTCGCG	30	2.1631258E-6	44.999996	32
AGTACGG	55	6.002665E-11	40.909092	2
CGGGTAC	50	1.0786607E-9	40.500004	6
CGTTTTT	1185	0.0	39.493668	1
CGAGAGG	75	0.0	39.0	2
ATAGTAG	35	6.24148E-6	38.57143	1
TACCACG	30	1.1390349E-4	37.499996	23
ACGCTAC	30	1.1390349E-4	37.499996	30
GGCCTAT	30	1.1390349E-4	37.499996	8
TAGTACG	30	1.1390349E-4	37.499996	1
CGTATGG	85	0.0	37.058823	2
TTAGCGG	110	0.0	36.81818	2
GCGTTAG	55	2.743036E-9	36.81818	1
TACGGGA	265	0.0	36.509434	4
AACGGGC	105	0.0	36.42857	4
>>END_MODULE