FastQCFastQC Report
Sat 18 Jun 2016
SRR3550631_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550631_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641839
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG47230.7358543186063795No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC39430.6143285154065116No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC33990.5295720577901935RNA PCR Primer, Index 30 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18490.28807847450840474No Hit
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC14870.2316780376387225Illumina Single End Adapter 2 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCT9410.14660997539881496No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTA8840.13772924362651695No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCGGAACT8160.12713468642447717No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6940.10812680438552348No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCC6920.10781519976193407No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA6620.103141130408093No Hit
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT6610.1029853280962983RNA PCR Primer, Index 30 (96% over 25bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6440.10033668879578836No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCG207.0309563E-445.020
TCGAGCA207.0309563E-445.014
CGGTCAA207.0309563E-445.031
GCTCGTC207.0309563E-445.039
CACGACG253.888875E-544.99999626
TACGGCT5250.041.5714267
CTCGAAT1150.041.0869643
CCGATGA6800.039.70588318
CGATGAA7150.039.6503519
CGCATAC403.455043E-739.37526
TAACGAA403.455043E-739.3751
AGGCACG403.455043E-739.3751
CATACGG403.455043E-739.3752
TGTAGCG403.455043E-739.3751
TTACCCG403.455043E-739.37526
CGAATGC1150.039.13043645
ACGGCTG5650.038.628328
ACCACCG9450.038.5714314
GACACGA356.2439776E-638.57142625
CGACAAA356.2439776E-638.57142612
Produced by FastQC (version 0.11.5)