##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550631_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	641839
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.233293707612034	33.0	31.0	34.0	30.0	34.0
2	32.36915955558948	34.0	31.0	34.0	30.0	34.0
3	32.33725903224952	34.0	31.0	34.0	30.0	34.0
4	35.91599295150341	37.0	35.0	37.0	35.0	37.0
5	36.00550137962947	37.0	35.0	37.0	35.0	37.0
6	36.03683010848515	37.0	35.0	37.0	35.0	37.0
7	36.0942697467745	37.0	36.0	37.0	35.0	37.0
8	36.06343802729345	37.0	36.0	37.0	35.0	37.0
9	37.87019641997448	39.0	38.0	39.0	35.0	39.0
10	37.553306981969	39.0	37.0	39.0	35.0	39.0
11	37.49574270183021	39.0	37.0	39.0	35.0	39.0
12	37.35763018451668	39.0	37.0	39.0	35.0	39.0
13	37.280902220027144	39.0	37.0	39.0	34.0	39.0
14	38.53877685837102	40.0	38.0	41.0	34.0	41.0
15	38.611083153251826	40.0	38.0	41.0	35.0	41.0
16	38.701848906034066	40.0	38.0	41.0	35.0	41.0
17	38.61051759086001	40.0	38.0	41.0	35.0	41.0
18	38.53771428660458	40.0	38.0	41.0	35.0	41.0
19	38.47708070092344	40.0	37.0	41.0	34.0	41.0
20	38.30154914238617	40.0	37.0	41.0	34.0	41.0
21	38.29088291612071	40.0	37.0	41.0	34.0	41.0
22	38.280997259437335	40.0	37.0	41.0	34.0	41.0
23	38.19066775312812	40.0	37.0	41.0	34.0	41.0
24	38.14506130041958	40.0	36.0	41.0	34.0	41.0
25	38.14718332790622	40.0	36.0	41.0	34.0	41.0
26	38.1083418115758	40.0	36.0	41.0	34.0	41.0
27	38.028198660411725	40.0	36.0	41.0	34.0	41.0
28	37.986124246111565	40.0	36.0	41.0	34.0	41.0
29	37.929951280617104	40.0	36.0	41.0	34.0	41.0
30	37.8463508761543	40.0	36.0	41.0	34.0	41.0
31	37.779563099157265	40.0	36.0	41.0	33.0	41.0
32	37.5873201846569	40.0	36.0	41.0	33.0	41.0
33	37.460624860751686	40.0	36.0	41.0	33.0	41.0
34	37.296220703322795	40.0	35.0	41.0	33.0	41.0
35	37.19983983522347	40.0	35.0	41.0	32.0	41.0
36	37.183547587479104	40.0	35.0	41.0	32.0	41.0
37	37.08028337324469	40.0	35.0	41.0	32.0	41.0
38	37.06762287738825	40.0	35.0	41.0	32.0	41.0
39	36.94643983927433	40.0	35.0	41.0	32.0	41.0
40	36.851680873240795	40.0	35.0	41.0	31.0	41.0
41	36.767327943612024	40.0	35.0	41.0	31.0	41.0
42	36.74372700942136	40.0	35.0	41.0	31.0	41.0
43	36.693189413544516	40.0	35.0	41.0	31.0	41.0
44	36.66834673492885	39.0	35.0	41.0	31.0	41.0
45	36.59416458021404	39.0	35.0	41.0	31.0	41.0
46	36.47818222326783	39.0	35.0	41.0	31.0	41.0
47	36.37093570194394	39.0	35.0	41.0	31.0	41.0
48	36.33976744946941	39.0	35.0	41.0	31.0	41.0
49	36.279951825925195	39.0	35.0	41.0	30.0	41.0
50	36.15851638806617	39.0	35.0	41.0	30.0	41.0
51	35.29607269112659	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	10.0
9	23.0
10	25.0
11	17.0
12	13.0
13	14.0
14	11.0
15	32.0
16	49.0
17	79.0
18	148.0
19	314.0
20	649.0
21	1381.0
22	2207.0
23	2880.0
24	3574.0
25	4439.0
26	5448.0
27	6058.0
28	6233.0
29	6813.0
30	7867.0
31	9967.0
32	12829.0
33	17442.0
34	35749.0
35	51476.0
36	37589.0
37	56877.0
38	107990.0
39	263270.0
40	362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1269196792342	21.49931680686278	24.42917928016216	11.94458423374086
2	32.155260119749656	28.815170159494823	24.207784195101887	14.821785525653631
3	31.83477476438795	27.478542126608076	25.260073008963307	15.426610100040664
4	28.471937666611097	25.093052930719384	28.886527618296796	17.548481784372715
5	29.379330330503446	29.008676630743846	23.570241135237964	18.041751903514744
6	26.128826699530567	37.06692799907765	23.094420875016944	13.709824426374839
7	77.0602908205952	6.790020550324925	11.847986800428146	4.3017018286517335
8	78.19624547589036	9.469197103946629	8.426100626481094	3.908456793681905
9	72.06838475069293	8.831186637147322	9.762261252432463	9.338167359727285
10	42.53403111995376	25.511070533264572	17.739495418633023	14.215402928148649
11	34.29956733698015	24.862465509263227	22.780479216750617	18.05748793700601
12	29.97620898698895	22.705849909400953	26.572551683521876	20.745389420088216
13	27.266495180255486	24.183479034461914	29.077385450245313	19.47264033503729
14	21.935874884511534	29.543078560199675	27.61767359104074	20.903372964248042
15	21.405991222097754	26.027866801487598	32.749801741558244	19.816340234856405
16	24.656027446135244	24.891288936945248	29.689532733286693	20.76315088363281
17	23.56447644970156	27.30310872352724	28.283728473963098	20.8486863528081
18	23.996672062620064	26.723679925962742	27.935977714037318	21.343670297379873
19	24.83348627926941	27.388644192702532	26.349442773031868	21.42842675499619
20	27.531826517241864	26.78257319982114	28.191960912316016	17.49363937062098
21	25.778115695680693	28.169057972482197	27.33333437201541	18.7194919598217
22	23.251625407617798	26.316724287554976	28.821090647343024	21.6105596574842
23	24.39739560855604	27.323207221748756	28.1366510916289	20.142746078066306
24	23.672603254087086	26.17728121849872	28.285753904016424	21.86436162339777
25	22.60302038361645	29.421864361623395	26.441054532367154	21.534060722392997
26	21.455692159560265	28.63693231480169	28.701746076508282	21.205629449129766
27	23.284655497718276	27.279582574446238	28.30834523922666	21.127416688608825
28	20.33562934006815	28.966921611182865	28.89712217549884	21.800326873250146
29	22.786243902287023	28.231690501823664	28.690528310059065	20.29153728583025
30	22.7486955451445	28.874842444912197	28.179964134307824	20.196497875635476
31	23.166089938442507	28.221251746933422	25.49471129052613	23.117947024097944
32	26.084734645292666	28.599851364594546	26.343522285183667	18.971891704929117
33	25.009387089285628	27.739510998864205	25.96242983053382	21.288672081316342
34	22.77892119363267	29.16541375640932	26.773536665737048	21.282128384220968
35	23.14303119629689	28.190247086886277	27.518583320739314	21.14813839607752
36	21.078338960393495	31.88883816658071	25.14057263581677	21.89225023720902
37	22.731089883911697	29.361101460023463	27.325544256425676	20.58226439963916
38	22.91150896096996	27.89344368291737	24.935225188871353	24.259822167241317
39	24.270105119819767	28.746149735369777	25.60875858275985	21.37498656205061
40	23.199587435478367	27.827071898092825	27.15805677124637	21.815283895182436
41	19.871650055543526	28.90460068646499	26.977949298811694	24.245799959179795
42	21.462703263591028	28.484401851554676	27.321493396319013	22.731401488535287
43	23.048770797661096	26.611813866094145	25.941708123065133	24.39770721317963
44	22.346725580714168	26.778054932779092	28.01091862601057	22.86430086049617
45	22.086224115393424	26.990725088378863	26.376084968348763	24.546965827878957
46	22.802914749649055	29.72708109042922	25.38113140522779	22.088872754693934
47	21.131155944091898	28.640515767972964	28.599383957659164	21.628944330275974
48	21.956908196603823	26.966731532362477	27.72533298849088	23.35102728254282
49	22.092300405553416	25.842929457387285	29.30065016304712	22.76411997401217
50	21.14377593134727	26.73988336638939	28.163449089257586	23.952891613005754
51	20.722174875630806	26.69314267285098	26.967354741609658	25.61732770990856
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	582.0
1	1088.0
2	1594.0
3	2989.0
4	4384.0
5	3103.5
6	1823.0
7	1779.0
8	1735.0
9	1749.5
10	1764.0
11	1778.0
12	1792.0
13	1861.5
14	1931.0
15	1947.0
16	1963.0
17	1949.5
18	1936.0
19	1838.5
20	1741.0
21	2147.0
22	2553.0
23	2718.5
24	2884.0
25	3693.0
26	5651.5
27	6801.0
28	8189.5
29	9578.0
30	11046.5
31	12515.0
32	13142.0
33	13769.0
34	15106.5
35	16444.0
36	18557.5
37	20671.0
38	22777.0
39	24883.0
40	27509.0
41	30135.0
42	33043.0
43	35951.0
44	38707.5
45	41464.0
46	51453.0
47	61442.0
48	57210.5
49	52979.0
50	51958.5
51	50938.0
52	47566.5
53	44195.0
54	40351.5
55	36508.0
56	34540.5
57	32573.0
58	31093.5
59	29614.0
60	29012.5
61	28411.0
62	25744.0
63	23077.0
64	18909.0
65	14741.0
66	11964.5
67	9188.0
68	7475.0
69	5762.0
70	4549.0
71	3336.0
72	3083.0
73	2830.0
74	2081.0
75	1081.0
76	830.0
77	586.5
78	343.0
79	259.5
80	176.0
81	130.5
82	85.0
83	71.5
84	58.0
85	35.0
86	12.0
87	10.5
88	9.0
89	5.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	641839.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.280517269580617
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.7056279421215	20.107276559403296
2	8.629273854886833	4.708221088443616
3	2.9603355066894608	2.422784517419836
4	1.5610146117419563	1.7034114429477651
5	1.0546164418914041	1.438524102790106
6	0.8592261614043254	1.4064080480799699
7	0.7329863114835071	1.399737201015441
8	0.6202122759034294	1.3535769362871524
9	0.5820177416084283	1.4289970546035935
>10	9.050843211308045	55.02867262149196
>50	0.19003955962231162	3.1940137693744877
>100	0.044647848345000775	2.175720829602749
>500	0.006296491433269339	1.2276980666286377
>1k	0.0028620415605769725	2.404957761911428
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG	4723	0.7358543186063795	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC	3943	0.6143285154065116	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC	3399	0.5295720577901935	RNA PCR Primer, Index 30 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1849	0.28807847450840474	No Hit
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	1487	0.2316780376387225	Illumina Single End Adapter 2 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCT	941	0.14660997539881496	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTA	884	0.13772924362651695	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCGGAACT	816	0.12713468642447717	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	694	0.10812680438552348	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCC	692	0.10781519976193407	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	662	0.103141130408093	No Hit
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	661	0.1029853280962983	RNA PCR Primer, Index 30 (96% over 25bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	644	0.10033668879578836	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10688038589116586	0.0
2	0.0	0.0	0.0	0.541880440421975	0.0
3	0.0	0.0	0.0	0.7143535995787106	0.0
4	0.0	0.0	0.0	1.0063271318819829	0.0
5	0.0	0.0	0.0	2.1164186034192376	0.0
6	0.0	0.0	0.0	2.482086629201404	0.0
7	1.558023117947024E-4	0.0	0.0	2.933913333406041	0.0
8	1.558023117947024E-4	0.0	0.0	3.439336032868056	0.0
9	1.558023117947024E-4	0.0	0.0	3.6133672151427385	0.0
10	1.558023117947024E-4	0.0	0.0	4.775340856507629	0.0
11	1.558023117947024E-4	0.0	0.0	5.548899334568326	0.0
12	1.558023117947024E-4	0.0	0.0	6.738294182809084	0.0
13	1.558023117947024E-4	0.0	0.0	7.010480821514429	0.0
14	3.116046235894048E-4	0.0	0.0	7.134655264014808	0.0
15	3.116046235894048E-4	0.0	0.0	7.488793918724166	0.0
16	3.116046235894048E-4	0.0	0.0	7.9412438321759815	0.0
17	4.674069353841072E-4	0.0	0.0	8.459130716581573	0.0
18	4.674069353841072E-4	0.0	0.0	8.956295893518469	0.0
19	4.674069353841072E-4	0.0	0.0	9.452214651961006	0.0
20	4.674069353841072E-4	0.0	0.0	9.811962189894974	0.0
21	4.674069353841072E-4	0.0	0.0	10.233874850235027	0.0
22	4.674069353841072E-4	0.0	0.0	10.72496373701193	0.0
23	4.674069353841072E-4	0.0	0.0	11.165728477079142	0.0
24	6.232092471788096E-4	0.0	0.0	11.526566631195674	0.0
25	6.232092471788096E-4	0.0	0.0	11.85281667209378	0.0
26	6.232092471788096E-4	0.0	0.0	12.15164550611602	0.0
27	6.232092471788096E-4	0.0	0.0	12.471196047606954	0.0
28	6.232092471788096E-4	0.0	0.0	12.765818219210736	0.0
29	6.232092471788096E-4	0.0	0.0	13.090198632367308	0.0
30	6.232092471788096E-4	0.0	0.0	13.433275322939242	0.0
31	6.232092471788096E-4	0.0	0.0	13.771989548780924	0.0
32	6.232092471788096E-4	0.0	0.0	14.110080565375428	0.0
33	6.232092471788096E-4	0.0	0.0	14.422152595900219	0.0
34	6.232092471788096E-4	0.0	0.0	14.76943594889061	0.0
35	7.79011558973512E-4	0.0	0.0	15.106124744678961	0.0
36	7.79011558973512E-4	0.0	0.0	15.448266621380128	0.0
37	9.348138707682144E-4	0.0	0.0	15.805053915389996	0.0
38	0.0010906161825629168	0.0	0.0	16.134264200212204	0.0
39	0.0010906161825629168	0.0	0.0	16.494479145081556	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATCG	20	7.0309563E-4	45.0	20
TCGAGCA	20	7.0309563E-4	45.0	14
CGGTCAA	20	7.0309563E-4	45.0	31
GCTCGTC	20	7.0309563E-4	45.0	39
CACGACG	25	3.888875E-5	44.999996	26
TACGGCT	525	0.0	41.571426	7
CTCGAAT	115	0.0	41.08696	43
CCGATGA	680	0.0	39.705883	18
CGATGAA	715	0.0	39.65035	19
CGCATAC	40	3.455043E-7	39.375	26
TAACGAA	40	3.455043E-7	39.375	1
AGGCACG	40	3.455043E-7	39.375	1
CATACGG	40	3.455043E-7	39.375	2
TGTAGCG	40	3.455043E-7	39.375	1
TTACCCG	40	3.455043E-7	39.375	26
CGAATGC	115	0.0	39.130436	45
ACGGCTG	565	0.0	38.62832	8
ACCACCG	945	0.0	38.57143	14
GACACGA	35	6.2439776E-6	38.571426	25
CGACAAA	35	6.2439776E-6	38.571426	12
>>END_MODULE