FastQCFastQC Report
Sat 18 Jun 2016
SRR3550630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672945
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC134141.993327835112825No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG133861.9891670195929831No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC87611.301889456047671No Hit
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC42880.6371991767529294TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34280.5094027000720712No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCT29360.43629122736627807No Hit
GAACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCT29000.43094160741219567No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGATTACTCGTA23560.35010290588383897No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTT21830.3243950099933873No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTC20870.3101293567825008No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGATTACT16990.25247234172183464No Hit
CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT14930.22186062754014071TruSeq Adapter, Index 27 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC12360.1836702850901634TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCC10500.15603058199407083No Hit
TCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC8850.1315114905378597TruSeq Adapter, Index 20 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8720.12957968333221884No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA8070.11992064730401443No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7420.11026161127581006No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6780.10075117580188574No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAT207.031199E-445.00000416
TTTACGG302.1641554E-645.0000042
AAACGTC207.031199E-445.00000418
CGGATCA207.031199E-445.00000421
GAAACGT207.031199E-445.00000417
ATGCGAA207.031199E-445.00000426
GCTACGA1350.045.00000410
TACACGA207.031199E-445.00000444
GCCCATA253.8890754E-545.09
ACACGAA253.8890754E-545.045
TAGCGAG253.8890754E-545.01
ACGTAAG253.8890754E-545.01
CCGATGA16650.043.1081118
CGATGAA17600.042.95454419
CGTAAGG900.042.5000042
CGATCCC850.042.35294334
CAAACGA800.042.18750415
GCGAACC700.041.78571733
GATGAAT19150.041.5926920
ACGGCTG9250.040.8648648
Produced by FastQC (version 0.11.5)