##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550630_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	672945
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9552712331617	33.0	31.0	34.0	30.0	34.0
2	32.088554042306576	33.0	31.0	34.0	30.0	34.0
3	32.07724256811478	33.0	31.0	34.0	30.0	34.0
4	35.710401295796835	37.0	35.0	37.0	35.0	37.0
5	35.768614076930504	37.0	35.0	37.0	35.0	37.0
6	35.809867076804196	37.0	35.0	37.0	35.0	37.0
7	35.86155777961052	37.0	35.0	37.0	35.0	37.0
8	35.760418756361965	37.0	35.0	37.0	35.0	37.0
9	37.54062070451523	39.0	37.0	39.0	35.0	39.0
10	37.32312150324321	39.0	37.0	39.0	34.0	39.0
11	37.261031733648366	39.0	37.0	39.0	34.0	39.0
12	37.13004628907266	39.0	37.0	39.0	34.0	39.0
13	37.017469481161164	39.0	37.0	39.0	33.0	39.0
14	38.23121057441544	40.0	37.0	41.0	33.0	41.0
15	38.27545341744125	40.0	37.0	41.0	33.0	41.0
16	38.43108723595539	40.0	37.0	41.0	34.0	41.0
17	38.26975161417352	40.0	37.0	41.0	34.0	41.0
18	38.23143347524686	40.0	37.0	41.0	34.0	41.0
19	38.19034393598288	40.0	37.0	41.0	34.0	41.0
20	37.84695183113033	40.0	36.0	41.0	33.0	41.0
21	37.898823826612876	40.0	36.0	41.0	33.0	41.0
22	37.8668969975258	40.0	36.0	41.0	33.0	41.0
23	37.7259196516803	40.0	36.0	41.0	33.0	41.0
24	37.778941815452974	40.0	36.0	41.0	33.0	41.0
25	37.823025655885694	40.0	36.0	41.0	33.0	41.0
26	37.71138354546063	40.0	36.0	41.0	33.0	41.0
27	37.51485336840307	40.0	35.0	41.0	33.0	41.0
28	37.4455133777649	40.0	35.0	41.0	33.0	41.0
29	37.40836175318934	40.0	35.0	41.0	32.0	41.0
30	37.351829644324575	40.0	35.0	41.0	32.0	41.0
31	37.30525080058548	40.0	35.0	41.0	32.0	41.0
32	36.95463670879492	40.0	35.0	41.0	31.0	41.0
33	36.810329224528004	40.0	35.0	41.0	31.0	41.0
34	36.6976409662008	40.0	35.0	41.0	31.0	41.0
35	36.60179063667908	40.0	35.0	41.0	30.0	41.0
36	36.528961505026416	40.0	35.0	41.0	30.0	41.0
37	36.34010060257525	39.0	35.0	41.0	30.0	41.0
38	36.32266827155265	39.0	35.0	41.0	30.0	41.0
39	36.225565239358346	39.0	35.0	41.0	30.0	41.0
40	36.156654704322044	39.0	35.0	41.0	29.0	41.0
41	36.0898082309847	39.0	35.0	41.0	28.0	41.0
42	36.00700800213985	39.0	35.0	41.0	29.0	41.0
43	35.96626470216734	39.0	35.0	41.0	28.0	41.0
44	35.974816664066154	39.0	35.0	41.0	28.0	41.0
45	35.977579148370225	39.0	35.0	41.0	28.0	41.0
46	35.87238481599536	39.0	35.0	41.0	28.0	41.0
47	35.60977791647163	38.0	35.0	41.0	27.0	41.0
48	35.632231460223345	38.0	35.0	41.0	27.0	41.0
49	35.591226623275304	38.0	35.0	41.0	27.0	41.0
50	35.453570499817964	38.0	35.0	41.0	27.0	41.0
51	34.62338526922706	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	7.0
9	9.0
10	16.0
11	14.0
12	12.0
13	8.0
14	26.0
15	24.0
16	48.0
17	67.0
18	194.0
19	547.0
20	1305.0
21	2680.0
22	4040.0
23	5194.0
24	5906.0
25	6390.0
26	6970.0
27	7428.0
28	7587.0
29	8378.0
30	9933.0
31	12837.0
32	16312.0
33	21409.0
34	38084.0
35	54237.0
36	44310.0
37	64751.0
38	113668.0
39	240276.0
40	274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.10853784484616	18.84908870710088	21.990058622918664	11.052314825134298
2	28.96596304304215	33.45518578784299	21.742638700042352	15.83621246907251
3	28.2767536722912	32.95811693377616	24.4910059514522	14.274123442480441
4	26.08504409721448	22.663813536024488	34.588710815891346	16.662431550869687
5	31.050085816820093	26.749437175400665	23.214972991849258	18.98550401592998
6	24.151453684922245	38.69796194339805	23.272481406355645	13.87810296532406
7	70.26413748523282	6.141809508949468	18.537919146438416	5.056133859379296
8	69.24013106568887	13.56812220909584	11.436595858502551	5.755150866712732
9	63.037543930038865	8.440957284770672	12.469666911857582	16.051831873332887
10	41.55109258557535	23.586920179212267	19.350021175578984	15.511966059633403
11	33.1137017141074	22.334217506631298	25.289733930707563	19.262346848553747
12	26.98496905393457	20.44030344233184	29.143540705406828	23.43118679832676
13	25.92351529471205	23.292393880629174	32.5172190892272	18.26687173543157
14	19.18997837861935	31.539576042618638	27.798557088617944	21.471888490144067
15	17.808290424923285	24.300648641419432	36.04559065005312	21.845470283604158
16	19.597143897346736	25.940009956237137	28.936688733848975	25.526157412567148
17	19.305143808186404	28.1462823856333	29.634665537302453	22.91390826887784
18	19.40842119341105	25.97507968704723	30.17646315820758	24.440035961334136
19	21.19400545364034	26.98422605116317	26.62357250592545	25.19819598927104
20	25.08823157910379	26.801298768844404	30.197118635252508	17.913351016799293
21	21.538164337352978	30.94903743990965	28.706803676377714	18.80599454635966
22	18.890845462853576	23.981157449717287	31.07742831880763	26.050568768621506
23	22.46454019273492	27.326007326007325	29.45411586385217	20.755336617405582
24	22.234952336372217	27.134609812094602	27.098202676295983	23.532235175237204
25	18.87553960576273	32.91041615585226	25.77298293322634	22.44106130515867
26	18.74387951467059	26.328897606788072	30.24348200818789	24.683740870353446
27	25.73196918024504	26.332464020090796	25.949966193373903	21.985600606290262
28	18.858747743129083	28.198292579631325	29.35128428029036	23.59167539694923
29	21.828529820416232	23.99066788519121	29.06166179999851	25.119140494394042
30	23.064589230917832	29.104904561294013	27.13223220322612	20.698274004562037
31	24.498138778057644	25.175311503911907	24.91005951452199	25.416490203508456
32	26.504394861392832	29.457830877709174	23.892740119920646	20.145034140977348
33	24.462920446693264	24.77914242620125	26.80456798103857	23.953369146066912
34	20.824733076254372	25.706409884908872	28.718394519611557	24.7504625192252
35	23.993639896276814	27.308769661710837	25.31217261440385	23.385417827608496
36	22.956110826293383	27.18557980221266	24.91199132172763	24.946318049766326
37	22.2052322255162	28.608875911107152	28.26367682351455	20.9222150398621
38	21.400411623535355	27.15481948747669	25.74519462957597	25.69957425941199
39	21.71737660581474	26.259352547385	29.68964774238608	22.33362310441418
40	20.251283537287595	25.937483746814376	30.768041964796527	23.043190751101502
41	19.14792442175809	26.664437658352462	26.150428341097715	28.037209578791728
42	20.304631136274136	26.855092169493794	29.039668917965063	23.800607776267004
43	23.071424856414716	23.866437821813076	26.01921405166841	27.042923270103795
44	22.915840076083484	23.802836784581206	28.3662112059678	24.915111933367513
45	21.36445028939958	23.655127833626818	27.516067434931536	27.464354442042072
46	24.152939690465043	27.657237961497593	26.28416883994977	21.905653508087585
47	19.64900549079048	25.162829057352383	33.25992465951898	21.928240792338155
48	21.598496162390685	24.425621707568972	28.114927668680206	25.86095446136014
49	20.94524812577551	23.007378017520004	32.32686177919444	23.72051207751005
50	20.099562371367647	23.420190357310034	30.20425146185795	26.27599580946437
51	20.851035374361945	22.99236936153772	27.386636352153594	28.76995891194674
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	332.0
1	918.5
2	1505.0
3	3647.0
4	5789.0
5	3876.5
6	1964.0
7	1911.5
8	1859.0
9	1899.5
10	1940.0
11	2097.5
12	2255.0
13	2322.0
14	2389.0
15	2321.0
16	2253.0
17	2231.5
18	2210.0
19	2135.0
20	2060.0
21	2182.0
22	2304.0
23	2620.5
24	2937.0
25	3753.0
26	4997.5
27	5426.0
28	6712.0
29	7998.0
30	9317.5
31	10637.0
32	11453.5
33	12270.0
34	13986.0
35	15702.0
36	16860.5
37	18019.0
38	18880.0
39	19741.0
40	22824.5
41	25908.0
42	29937.0
43	33966.0
44	38140.5
45	42315.0
46	72361.5
47	102408.0
48	84271.0
49	66134.0
50	62870.0
51	59606.0
52	51218.5
53	42831.0
54	39735.5
55	36640.0
56	33407.0
57	30174.0
58	28300.0
59	26426.0
60	24436.0
61	22446.0
62	20111.0
63	17776.0
64	16203.0
65	14630.0
66	11771.5
67	8913.0
68	7756.5
69	6600.0
70	5689.5
71	4779.0
72	3857.5
73	2936.0
74	2496.0
75	1641.0
76	1226.0
77	888.0
78	550.0
79	430.5
80	311.0
81	199.0
82	87.0
83	65.5
84	44.0
85	30.5
86	17.0
87	9.0
88	1.0
89	2.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	672945.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.156673172641156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.90091033059716	21.838613628425232
2	10.139549207764045	5.912710447373777
3	3.6336388538679447	3.1783446146891423
4	1.6725750056238227	1.9506689118280895
5	1.0242079598216791	1.4931248372669141
6	0.7530113338723843	1.3173183214207007
7	0.5529460754135769	1.1285447602049774
8	0.44965364234286975	1.0488323432542985
9	0.40668313732130856	1.0671774587731562
>10	6.075988142224145	41.952422700082614
>50	0.3167562260309581	5.609707574199304
>100	0.061307656651120236	3.2746010675686175
>500	0.005619868526352688	1.109011733978454
>1k	0.005619868526352688	3.8217333844125205
>5k	5.108971387593353E-4	1.3050438954177617
>10k+	0.0010217942775186705	3.9921443211044414
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC	13414	1.993327835112825	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG	13386	1.9891670195929831	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC	8761	1.301889456047671	No Hit
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	4288	0.6371991767529294	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3428	0.5094027000720712	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCT	2936	0.43629122736627807	No Hit
GAACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCT	2900	0.43094160741219567	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGATTACTCGTA	2356	0.35010290588383897	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTT	2183	0.3243950099933873	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTC	2087	0.3101293567825008	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGATTACT	1699	0.25247234172183464	No Hit
CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT	1493	0.22186062754014071	TruSeq Adapter, Index 27 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	1236	0.1836702850901634	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCC	1050	0.15603058199407083	No Hit
TCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	885	0.1315114905378597	TruSeq Adapter, Index 20 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	872	0.12957968333221884	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	807	0.11992064730401443	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	742	0.11026161127581006	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	678	0.10075117580188574	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.972011085601349E-4	0.0	0.0	0.22988505747126436	0.0
2	2.972011085601349E-4	0.0	0.0	1.3133316987272363	0.0
3	2.972011085601349E-4	0.0	0.0	1.6774030567134015	0.0
4	2.972011085601349E-4	0.0	0.0	2.535868458789351	0.0
5	2.972011085601349E-4	0.0	0.0	5.790071996968549	0.0
6	2.972011085601349E-4	0.0	0.0	6.7849527078736	0.0
7	2.972011085601349E-4	0.0	0.0	7.952358662297811	0.0
8	2.972011085601349E-4	0.0	0.0	9.298976885183782	0.0
9	2.972011085601349E-4	0.0	0.0	9.707925610562528	0.0
10	2.972011085601349E-4	0.0	0.0	12.687961126095	0.0
11	2.972011085601349E-4	0.0	0.0	14.148853175222344	0.0
12	4.458016628402024E-4	0.0	0.0	16.51947781765226	0.0
13	4.458016628402024E-4	0.0	0.0	17.03200112936421	0.0
14	4.458016628402024E-4	0.0	0.0	17.2728826278522	0.0
15	4.458016628402024E-4	0.0	0.0	17.972345436848478	0.0
16	4.458016628402024E-4	0.0	0.0	18.675820460810318	0.0
17	4.458016628402024E-4	0.0	0.0	19.481384065562565	0.0
18	4.458016628402024E-4	0.0	0.0	20.26971000601832	0.0
19	4.458016628402024E-4	0.0	0.0	21.114801358209068	0.0
20	4.458016628402024E-4	0.0	0.0	21.69553232433557	0.0
21	4.458016628402024E-4	0.0	0.0	22.317871445660494	0.0
22	4.458016628402024E-4	0.0	0.0	23.026547489022136	0.0
23	4.458016628402024E-4	0.0	0.0	23.6552764341811	0.0
24	4.458016628402024E-4	0.0	0.0	24.175526974715616	0.0
25	5.944022171202698E-4	0.0	0.0	24.6207342353387	0.0
26	5.944022171202698E-4	0.0	0.0	25.08927178298375	0.0
27	5.944022171202698E-4	0.0	0.0	25.55275691178328	0.0
28	5.944022171202698E-4	0.0	0.0	25.959625229402107	0.0
29	5.944022171202698E-4	0.0	0.0	26.41493732771623	0.0
30	5.944022171202698E-4	0.0	0.0	26.899375134669253	0.0
31	5.944022171202698E-4	0.0	0.0	27.344136593629493	0.0
32	5.944022171202698E-4	0.0	0.0	27.77463239937885	0.0
33	7.430027714003374E-4	0.0	0.0	28.17511089316363	0.0
34	7.430027714003374E-4	0.0	0.0	28.5853970235309	0.0
35	7.430027714003374E-4	0.0	0.0	28.996871958332406	0.0
36	7.430027714003374E-4	0.0	0.0	29.425584557430398	0.0
37	7.430027714003374E-4	0.0	0.0	29.85177094710563	0.0
38	7.430027714003374E-4	0.0	0.0	30.255667253638855	0.0
39	7.430027714003374E-4	0.0	0.0	30.70369792479326	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAT	20	7.031199E-4	45.000004	16
TTTACGG	30	2.1641554E-6	45.000004	2
AAACGTC	20	7.031199E-4	45.000004	18
CGGATCA	20	7.031199E-4	45.000004	21
GAAACGT	20	7.031199E-4	45.000004	17
ATGCGAA	20	7.031199E-4	45.000004	26
GCTACGA	135	0.0	45.000004	10
TACACGA	20	7.031199E-4	45.000004	44
GCCCATA	25	3.8890754E-5	45.0	9
ACACGAA	25	3.8890754E-5	45.0	45
TAGCGAG	25	3.8890754E-5	45.0	1
ACGTAAG	25	3.8890754E-5	45.0	1
CCGATGA	1665	0.0	43.10811	18
CGATGAA	1760	0.0	42.954544	19
CGTAAGG	90	0.0	42.500004	2
CGATCCC	85	0.0	42.352943	34
CAAACGA	80	0.0	42.187504	15
GCGAACC	70	0.0	41.785717	33
GATGAAT	1915	0.0	41.59269	20
ACGGCTG	925	0.0	40.864864	8
>>END_MODULE