##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550626_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	646988
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23505381861796	33.0	31.0	34.0	30.0	34.0
2	32.369640549747444	34.0	31.0	34.0	30.0	34.0
3	32.34287807501839	34.0	31.0	34.0	30.0	34.0
4	35.931385126153806	37.0	35.0	37.0	35.0	37.0
5	35.99899534458135	37.0	35.0	37.0	35.0	37.0
6	36.042884875762766	37.0	35.0	37.0	35.0	37.0
7	36.10615652840547	37.0	36.0	37.0	35.0	37.0
8	36.07059327220907	37.0	36.0	37.0	35.0	37.0
9	37.82648828108095	39.0	38.0	39.0	35.0	39.0
10	37.587284462772104	39.0	37.0	39.0	35.0	39.0
11	37.49570007480819	39.0	37.0	39.0	35.0	39.0
12	37.41709738047692	39.0	37.0	39.0	35.0	39.0
13	37.380575837573495	39.0	37.0	39.0	35.0	39.0
14	38.7144444719222	40.0	38.0	41.0	35.0	41.0
15	38.7852464033336	40.0	38.0	41.0	35.0	41.0
16	38.870790493795866	40.0	38.0	41.0	35.0	41.0
17	38.73070443346708	40.0	38.0	41.0	35.0	41.0
18	38.68814413868572	40.0	38.0	41.0	35.0	41.0
19	38.61931442314231	40.0	38.0	41.0	35.0	41.0
20	38.29485245475959	40.0	37.0	41.0	34.0	41.0
21	38.40685453207787	40.0	37.0	41.0	34.0	41.0
22	38.40448972778475	40.0	37.0	41.0	34.0	41.0
23	38.183930768422286	40.0	37.0	41.0	34.0	41.0
24	38.24972178773022	40.0	37.0	41.0	34.0	41.0
25	38.31063481857469	40.0	37.0	41.0	34.0	41.0
26	38.19778728508102	40.0	37.0	41.0	34.0	41.0
27	38.061922323134276	40.0	37.0	41.0	34.0	41.0
28	38.083094276864486	40.0	37.0	41.0	34.0	41.0
29	37.99300450703877	40.0	37.0	41.0	34.0	41.0
30	37.95024482679741	40.0	37.0	41.0	33.0	41.0
31	37.83595368074833	40.0	37.0	41.0	33.0	41.0
32	37.5278552306998	40.0	36.0	41.0	33.0	41.0
33	37.456289761170225	40.0	36.0	41.0	33.0	41.0
34	37.29035623535521	40.0	36.0	41.0	32.0	41.0
35	37.20905333638336	40.0	36.0	41.0	32.0	41.0
36	37.187253859422434	40.0	36.0	41.0	32.0	41.0
37	36.944006689459464	40.0	36.0	41.0	31.0	41.0
38	36.91346052786141	40.0	36.0	41.0	31.0	41.0
39	36.89042918879485	40.0	36.0	41.0	31.0	41.0
40	36.7926221197302	40.0	35.0	41.0	31.0	41.0
41	36.82064582341558	40.0	35.0	41.0	30.0	41.0
42	36.634654738573204	40.0	35.0	41.0	30.0	41.0
43	36.53884461535608	40.0	35.0	41.0	30.0	41.0
44	36.562899157325944	40.0	35.0	41.0	30.0	41.0
45	36.50023957167675	40.0	35.0	41.0	30.0	41.0
46	36.45315678188776	39.0	35.0	41.0	30.0	41.0
47	36.29724044340853	39.0	35.0	41.0	30.0	41.0
48	36.309776997409536	39.0	35.0	41.0	30.0	41.0
49	36.24794122920363	39.0	35.0	41.0	30.0	41.0
50	36.10117189190526	39.0	35.0	41.0	29.0	41.0
51	35.26897716804639	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	8.0
10	11.0
11	12.0
12	12.0
13	9.0
14	15.0
15	16.0
16	37.0
17	83.0
18	198.0
19	441.0
20	1081.0
21	2405.0
22	3861.0
23	4416.0
24	4860.0
25	5279.0
26	5897.0
27	6140.0
28	6295.0
29	6574.0
30	7268.0
31	8896.0
32	11420.0
33	15110.0
34	28491.0
35	44369.0
36	36118.0
37	54091.0
38	108740.0
39	284438.0
40	391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.68877320754017	19.396959449016055	24.274947912480602	11.639319430963171
2	30.346776138042745	28.875806042770503	24.9137541963684	15.863663622818352
3	30.52900517474822	28.031122679245986	26.45505017094598	14.984821975059814
4	26.08610978874415	24.7449720860356	31.88219874248054	17.286719382739708
5	29.70178735927096	29.2158432613897	23.954230990373855	17.128138388965482
6	25.604338874909583	37.8595893586898	22.66919942873747	13.86687233766314
7	77.77825863849097	5.451105739210001	12.886483211435143	3.88415241086388
8	79.62976129387252	9.629235781807392	7.409256431340302	3.3317464929797773
9	73.47570588635338	7.835230328846903	8.697688365162879	9.99137541963684
10	39.897030547707224	34.103260029552324	14.68079778913983	11.318911633600623
11	30.724372013082156	24.52564808002621	27.821845227423076	16.928134679468553
12	26.577772694393097	22.583880999338472	29.486327412564066	21.352018893704365
13	26.26679320172863	23.811260796181692	31.475545141486393	18.446400860603287
14	19.573315115581742	31.305990219293093	28.761429887416767	20.359264777708397
15	18.65830587275189	26.49786394801758	33.32673867212375	21.517091507106777
16	21.829152936375944	26.181320209957526	30.422202575627367	21.56732427803916
17	21.445838253568844	28.230662701626613	29.295752007765213	21.027747037039326
18	21.66191644976414	25.61840405077065	29.94336834686269	22.77631115260252
19	23.160244084898018	26.891843434499556	27.5125659208517	22.43534655975072
20	28.256320055395157	25.486098660253358	29.428830210143005	16.828751074208487
21	23.53505783723964	29.248301359530625	28.14812639492541	19.068514408304328
22	22.224523484206816	24.635696488961155	30.904282614206135	22.23549741262589
23	24.014819440236913	27.442085479174267	29.153863750177745	19.389231330411075
24	21.768255361768688	27.47871676136188	29.09729392198928	21.65573395488015
25	20.764372754981544	30.878007010949197	27.89371673044941	20.46390350361985
26	20.903015202754922	28.566217611454924	30.7495656797344	19.781201506055755
27	24.358720718158605	28.107476491063206	27.788459755049555	19.745343035728638
28	18.860164330714017	31.647573061633295	29.79127897271665	19.700983634936044
29	21.85743785047018	27.4281748656853	30.828392489505212	19.885994794339307
30	22.060687369781203	29.294051821672117	28.793269736069295	19.851991072477386
31	22.211694807322544	27.77269439309539	26.510074375413456	23.505536424168607
32	25.49444502834674	29.27921383395055	26.339437516615455	18.886903621087253
33	25.555960852442393	27.583819174389635	25.749936629427438	21.110283343740534
34	21.451866186080732	29.05911701608067	26.986435606224536	22.502581191614063
35	24.4894804849549	27.084428150135704	27.23079871651406	21.195292648395334
36	21.933173412799	32.684068328933456	25.381150809597703	20.001607448669837
37	22.77646571497462	29.521103946286487	27.297415098889005	20.405015239849888
38	21.81817900795687	29.035468973149424	25.857821165152984	23.288530853740717
39	22.260227392161834	28.835001576536197	26.464787600388263	22.43998343091371
40	22.98867985186742	26.841765225939273	27.878878742727842	22.290676179465464
41	20.933773114802747	27.680111532207707	26.809307127798355	24.576808225191193
42	22.16841734313465	29.10053973180337	27.271757745120468	21.459285179941514
43	24.56088830086493	27.8955714789146	26.139897494234827	21.403642725985645
44	22.800577445022164	27.56094394331889	27.71287875509283	21.92559985656612
45	21.503490018362008	27.442085479174267	26.788750332309103	24.265674170154625
46	23.18605600103866	29.41136466209574	26.03170383376508	21.37087550310052
47	21.080452805925304	29.45927899744663	28.811971783093348	20.648296413534716
48	21.846309359679005	27.89170741961211	27.642398313415395	22.61958490729349
49	21.30781405528387	27.281031487446445	29.50224733689033	21.908907120379357
50	21.324661353842732	27.527713033317465	27.774549141560584	23.37307647127922
51	20.86561110870681	26.984426295387244	27.23218977786296	24.917772818042994
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	247.0
1	842.0
2	1437.0
3	3248.5
4	5060.0
5	3597.0
6	2134.0
7	2071.5
8	2009.0
9	1964.5
10	1920.0
11	1901.5
12	1883.0
13	1863.5
14	1844.0
15	1885.0
16	1926.0
17	1816.0
18	1706.0
19	1843.5
20	1981.0
21	2427.0
22	2873.0
23	3122.0
24	3371.0
25	4078.5
26	6601.0
27	8416.0
28	9789.0
29	11162.0
30	12453.5
31	13745.0
32	15075.0
33	16405.0
34	18650.5
35	20896.0
36	22863.5
37	24831.0
38	26446.5
39	28062.0
40	31083.0
41	34104.0
42	35954.5
43	37805.0
44	39729.5
45	41654.0
46	52285.5
47	62917.0
48	57275.0
49	51633.0
50	50391.5
51	49150.0
52	45426.0
53	41702.0
54	39521.0
55	37340.0
56	34400.5
57	31461.0
58	29674.5
59	27888.0
60	26553.5
61	25219.0
62	21904.0
63	18589.0
64	15563.5
65	12538.0
66	10006.5
67	7475.0
68	5927.5
69	4380.0
70	3575.5
71	2771.0
72	2255.0
73	1739.0
74	1244.0
75	736.5
76	724.0
77	509.0
78	294.0
79	188.0
80	82.0
81	68.5
82	55.0
83	31.5
84	8.0
85	4.5
86	1.0
87	2.5
88	4.0
89	2.0
90	0.0
91	1.0
92	2.0
93	2.5
94	3.0
95	5.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	646988.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.80505635802261
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.72584161277484	19.762253394730784
2	8.162760375265705	4.376065037920621
3	2.8295426990385852	2.275381545454821
4	1.4866215329151355	1.593958958913607
5	1.039314469925276	1.3929441470027715
6	0.8352186566660874	1.3432849897923846
7	0.700944384172947	1.3152197605116707
8	0.6614499890161948	1.4184163386874031
9	0.609536307717458	1.470478957256473
>10	9.7306628446581	56.61833120179257
>50	0.1735600486867506	2.8889150803176857
>100	0.03586907672859505	1.6965339512950472
>500	0.005206801460602508	1.0267597158614723
>1k	0.0028926674781125043	1.9793929939972559
>5k	5.785334956225009E-4	0.8420639264654577
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCG	5430	0.8392736805010295	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGC	3862	0.5969198810487985	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC	3705	0.5726535886291555	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2493	0.38532399364439524	No Hit
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	1441	0.22272437819557703	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAAGCAGT	1263	0.19521227596184165	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTA	985	0.15224393651814253	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCT	963	0.1488435643319505	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	832	0.12859589358689807	No Hit
CTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGCT	689	0.10649347437664995	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTC	676	0.10448416353935468	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCC	659	0.10185660321366083	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCACCAGTCGAATGC	652	0.10077466660896339	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.182494883985484E-4	0.0	0.0	0.11514896721422963	0.0
2	6.182494883985484E-4	0.0	0.0	0.5139198872312933	0.0
3	6.182494883985484E-4	0.0	0.0	0.6425157808181914	0.0
4	6.182494883985484E-4	0.0	0.0	0.8895064514334114	0.0
5	6.182494883985484E-4	0.0	0.0	1.8844244406387753	0.0
6	6.182494883985484E-4	0.0	0.0	2.1556814036736385	0.0
7	6.182494883985484E-4	0.0	0.0	2.5723815588542602	0.0
8	6.182494883985484E-4	0.0	0.0	3.0685267732940953	0.0
9	6.182494883985484E-4	0.0	0.0	3.243491378510884	0.0
10	6.182494883985484E-4	0.0	0.0	4.509820893123211	0.0
11	6.182494883985484E-4	0.0	0.0	5.214007060409157	0.0
12	6.182494883985484E-4	0.0	0.0	6.388526525994299	0.0
13	6.182494883985484E-4	0.0	0.0	6.649273247726388	0.0
14	6.182494883985484E-4	0.0	0.0	6.772459458289799	0.0
15	6.182494883985484E-4	0.0	0.0	7.113887738257897	0.0
16	6.182494883985484E-4	0.0	0.0	7.514204281995957	0.0
17	6.182494883985484E-4	0.0	0.0	7.9826828318299565	0.0
18	6.182494883985484E-4	0.0	0.0	8.438641829523887	0.0
19	6.182494883985484E-4	0.0	0.0	8.98254681694251	0.0
20	6.182494883985484E-4	0.0	0.0	9.308518859700644	0.0
21	6.182494883985484E-4	0.0	0.0	9.678232053762976	0.0
22	6.182494883985484E-4	0.0	0.0	10.113016006479254	0.0
23	6.182494883985484E-4	0.0	0.0	10.506377243472832	0.0
24	6.182494883985484E-4	0.0	0.0	10.825084854742283	0.0
25	6.182494883985484E-4	0.0	0.0	11.100205877079636	0.0
26	6.182494883985484E-4	0.0	0.0	11.355542915788238	0.0
27	6.182494883985484E-4	0.0	0.0	11.646120175335556	0.0
28	6.182494883985484E-4	0.0	0.0	11.930205815254688	0.0
29	7.728118604981854E-4	0.0	0.0	12.2232560727556	0.0
30	7.728118604981854E-4	0.0	0.0	12.558965544956012	0.0
31	7.728118604981854E-4	0.0	0.0	12.876436657248666	0.0
32	7.728118604981854E-4	0.0	0.0	13.182161029261747	0.0
33	7.728118604981854E-4	0.0	0.0	13.47938447080935	0.0
34	7.728118604981854E-4	0.0	0.0	13.785417967566632	0.0
35	7.728118604981854E-4	0.0	0.0	14.099488707673094	0.0
36	7.728118604981854E-4	0.0	0.0	14.439835051036495	0.0
37	7.728118604981854E-4	0.0	0.0	14.767043592771428	0.0
38	7.728118604981854E-4	0.0	0.0	15.105844312413831	0.0
39	7.728118604981854E-4	0.0	0.0	15.436453226334955	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	25	3.8889077E-5	45.000004	1
CGACGGT	50	2.1827873E-11	45.000004	28
CCGTCGA	90	0.0	45.000004	41
CCCGTCG	100	0.0	45.000004	40
CTTGCGT	25	3.8889077E-5	45.000004	15
GCGATTC	30	2.1640244E-6	45.000004	9
TGTAGCG	45	3.8380676E-10	45.000004	1
CGTCGAA	90	0.0	45.000004	42
CGGTCTA	50	2.1827873E-11	45.000004	31
CCGCGTA	25	3.8889077E-5	45.000004	40
CGGTAGT	100	0.0	45.000004	12
TAGCGCG	35	1.2108285E-7	45.0	1
CCTACCG	20	7.030998E-4	45.0	37
CGTAAGC	55	1.8189894E-12	45.0	43
CCGCATT	20	7.030998E-4	45.0	40
GCCCGTC	20	7.030998E-4	45.0	20
TACCGGT	20	7.030998E-4	45.0	39
CGATGAA	1500	0.0	43.2	19
ACCCGTC	155	0.0	42.09677	39
CGTATGG	75	0.0	42.0	2
>>END_MODULE