FastQCFastQC Report
Sat 18 Jun 2016
SRR3550623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222285
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70780.5790793472880711No Hit
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC31210.25534143019017663No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC28820.23578788907660653No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG27110.2217976985727551No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT20910.17107303124884948No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC18700.15299214176726375No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC17640.144319859934467No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCC15820.12942971565551406No Hit
TCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC13250.10840352290995962No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG253.8909515E-545.024
CGATTCG253.8909515E-545.010
TCGTGCG502.1827873E-1145.01
CGTTTTT40000.041.4562531
GCTAGCG950.040.263161
ACGCCGC451.9284926E-840.014
TCTAGCG451.9284926E-840.01
AGTTGCG451.9284926E-840.01
TATCGCG451.9284926E-840.01
TCGGTCG403.4584627E-739.3751
TAACGCC356.248614E-638.5714312
CGCACGG1350.038.3333322
CTAACGG659.094947E-1238.076922
CGAATAT1300.038.0769214
TTTCGCG301.13996444E-437.4999961
CGCGATT301.13996444E-437.4999967
AATAGCG601.5643309E-1037.4999961
TAGGGCG2800.036.9642875
GCTACGA1350.036.66666410
TATACGG800.036.56252