##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550623_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1222285
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23075714747379	33.0	31.0	34.0	30.0	34.0
2	32.36494270976082	34.0	31.0	34.0	30.0	34.0
3	32.3176370486425	34.0	31.0	34.0	30.0	34.0
4	35.89688820528764	37.0	35.0	37.0	35.0	37.0
5	36.02239003178473	37.0	35.0	37.0	35.0	37.0
6	36.07029866193236	37.0	35.0	37.0	35.0	37.0
7	36.21438207946592	37.0	37.0	37.0	35.0	37.0
8	36.21172885210896	37.0	37.0	37.0	35.0	37.0
9	38.03189763434878	39.0	39.0	39.0	35.0	39.0
10	37.668955276388076	39.0	38.0	39.0	35.0	39.0
11	37.560846283804516	39.0	37.0	39.0	35.0	39.0
12	37.54534907979726	39.0	37.0	39.0	35.0	39.0
13	37.58921446307531	39.0	37.0	39.0	35.0	39.0
14	38.86977996130199	40.0	38.0	41.0	35.0	41.0
15	38.957464093889726	40.0	38.0	41.0	35.0	41.0
16	38.993505606302946	40.0	38.0	41.0	35.0	41.0
17	38.936577803049204	40.0	38.0	41.0	35.0	41.0
18	38.84056009850403	40.0	38.0	41.0	35.0	41.0
19	38.77452476304626	40.0	38.0	41.0	35.0	41.0
20	38.68553651562443	40.0	38.0	41.0	35.0	41.0
21	38.63203344555484	40.0	38.0	41.0	35.0	41.0
22	38.6566414543253	40.0	38.0	41.0	35.0	41.0
23	38.612111741533276	40.0	38.0	41.0	35.0	41.0
24	38.546558290415085	40.0	38.0	41.0	35.0	41.0
25	38.49357473911567	40.0	38.0	41.0	35.0	41.0
26	38.52906237088732	40.0	38.0	41.0	35.0	41.0
27	38.49644886421743	40.0	38.0	41.0	35.0	41.0
28	38.471956213158144	40.0	38.0	41.0	35.0	41.0
29	38.43468094593323	40.0	38.0	41.0	35.0	41.0
30	38.32546991904507	40.0	37.0	41.0	34.0	41.0
31	38.26851757159746	40.0	37.0	41.0	34.0	41.0
32	38.105409131258256	40.0	37.0	41.0	34.0	41.0
33	37.9751064604409	40.0	37.0	41.0	34.0	41.0
34	37.860179090801246	40.0	37.0	41.0	33.0	41.0
35	37.8144589846067	40.0	37.0	41.0	33.0	41.0
36	37.774305501581054	40.0	37.0	41.0	34.0	41.0
37	37.7496590402402	40.0	37.0	41.0	34.0	41.0
38	37.70795354602241	40.0	37.0	41.0	33.0	41.0
39	37.63888945704152	40.0	37.0	41.0	33.0	41.0
40	37.571527098835375	40.0	37.0	41.0	33.0	41.0
41	37.500380844074826	40.0	36.0	41.0	33.0	41.0
42	37.50190503851393	40.0	36.0	41.0	33.0	41.0
43	37.441069799596654	40.0	36.0	41.0	33.0	41.0
44	37.37823175445988	40.0	36.0	41.0	33.0	41.0
45	37.295637269540244	40.0	36.0	41.0	33.0	41.0
46	37.21492777870955	40.0	36.0	41.0	33.0	41.0
47	37.16741676450255	40.0	36.0	41.0	32.0	41.0
48	37.138439889223875	40.0	35.0	41.0	32.0	41.0
49	37.107627926383785	40.0	35.0	41.0	32.0	41.0
50	37.02392977087995	40.0	35.0	41.0	32.0	41.0
51	36.134259194868626	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	12.0
10	20.0
11	22.0
12	17.0
13	13.0
14	21.0
15	32.0
16	50.0
17	87.0
18	164.0
19	357.0
20	581.0
21	977.0
22	1686.0
23	2549.0
24	4022.0
25	6154.0
26	9008.0
27	10512.0
28	11094.0
29	11665.0
30	13384.0
31	16099.0
32	20610.0
33	28370.0
34	55177.0
35	79154.0
36	67932.0
37	102151.0
38	209955.0
39	569664.0
40	735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.83930507205766	21.423399616292436	26.86681093198395	12.870484379665953
2	32.31496745848963	24.651370179622592	26.359809700683556	16.67385266120422
3	30.5180052115505	24.191984684423026	29.303067615163403	15.98694248886307
4	27.391647610827263	25.00677010680815	29.51529307812826	18.086289204236326
5	25.69613469853594	29.20660893326843	26.556899577430798	18.54035679076484
6	26.212708165444234	33.316616010177654	25.614484346940365	14.856191477437749
7	81.07814462257166	5.017160482211596	9.744208592922272	4.160486302294473
8	83.47513059556486	4.562602011805757	8.357216197531672	3.605051195097706
9	76.88190561121179	7.62309935898747	10.094945123273213	5.400049906527529
10	34.54480747125261	39.060202816855316	16.12921699930867	10.265772712583399
11	24.157213743112287	23.60112412407908	34.547670960537026	17.693991172271605
12	23.82120372908119	22.1097370907767	34.982675889829295	19.086383290312817
13	22.532060853237994	23.181172967024878	35.717856310107706	18.568909869629422
14	19.115018183156955	27.16224121215592	33.85830636880924	19.864434235877884
15	18.890356995299786	26.747362521834106	35.89833794900535	18.46394253386076
16	22.25356606683384	27.14489664849033	32.36307407846778	18.238463206208046
17	21.160204044065008	26.035826341646995	31.903770397247776	20.900199217040218
18	21.728156690133645	26.095468732742365	32.83882236957829	19.337552207545702
19	21.018747673414957	27.907893821817336	31.273557312738028	19.79980119202968
20	23.683592615470207	27.503323692919405	31.44070327296825	17.372380418642134
21	22.782657072614	27.28496218148795	31.86605415267307	18.066326593224986
22	21.127969336120465	24.39103809667958	32.60254359662436	21.878448970575604
23	20.010963073260328	26.535627942746576	32.759626437369356	20.693782546623744
24	21.52174001971717	25.29786424606372	32.49733081891703	20.683064915302076
25	20.119857480047614	27.672515002638505	30.34685036632209	21.86077715099179
26	18.98886102668363	28.489918472369375	31.636484126042618	20.88473637490438
27	19.36635072834895	27.688141472733445	31.423195081343547	21.522312717574053
28	17.840356381694942	28.09238434571315	32.43417042670081	21.633088845891095
29	19.8900420114785	25.042441001893994	33.235783798377625	21.831733188249878
30	20.91721652478759	25.168843600305983	32.20787295925255	21.706066915653878
31	21.069554154718418	26.620305411585676	29.807696241056707	22.502444192639196
32	21.683568071276337	26.200354254531472	30.79928167325951	21.31679600093268
33	21.81152513529987	25.86630777600969	29.95054344935919	22.371623639331254
34	19.656217657911206	26.120831066404314	32.14004917020171	22.082902105482763
35	18.86973987245201	25.76125862626147	31.503290967327587	23.865710533958936
36	19.732877356753946	27.073309416379978	30.765901569601194	22.427911657264875
37	19.590521032328795	27.341250199421573	30.94900125584459	22.119227512405047
38	19.69589743799523	27.027166331911133	29.67016694142528	23.606769288668357
39	21.6444609890492	26.08516017131847	30.757065659809292	21.513313179823037
40	21.53867551348499	25.61996588357053	30.46449886892173	22.37685973402275
41	18.559174006062413	26.762907177949497	30.38939363569053	24.288525180297558
42	19.537260131638693	25.667254363753134	30.976736194913624	23.81874930969455
43	20.34615494749588	24.84265126382145	31.13357359371996	23.677620194962714
44	20.231615376119315	25.175879602547685	30.341859713569256	24.250645307763737
45	20.760542753940367	24.954327345913597	30.05043831839546	24.23469158175057
46	21.366129830604155	25.905742114155046	29.641286606642474	23.08684144859832
47	18.41665405367815	26.129094278339338	31.986975214454894	23.467276453527614
48	19.19503225516144	24.086444650797482	32.06453486707274	24.653988226968345
49	20.30393893404566	23.72662676871597	32.65040477466385	23.31902952257452
50	19.49267151278139	24.048646592243216	31.767713749248333	24.69096814572706
51	18.642460637249087	23.974850382684888	30.471780313102098	26.910908666963923
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	458.0
1	1171.0
2	1884.0
3	7281.5
4	12679.0
5	8369.5
6	4060.0
7	4021.5
8	3983.0
9	3980.5
10	3978.0
11	3952.5
12	3927.0
13	3953.0
14	3979.0
15	3812.0
16	3645.0
17	3831.5
18	4018.0
19	4028.0
20	4038.0
21	4671.5
22	5305.0
23	6288.0
24	7271.0
25	8702.5
26	12651.0
27	15168.0
28	18038.5
29	20909.0
30	24091.5
31	27274.0
32	31370.5
33	35467.0
34	41347.5
35	47228.0
36	49333.5
37	51439.0
38	53963.5
39	56488.0
40	63513.0
41	70538.0
42	74253.5
43	77969.0
44	82512.5
45	87056.0
46	100737.0
47	114418.0
48	116409.0
49	118400.0
50	112803.0
51	107206.0
52	93452.5
53	79699.0
54	70445.5
55	61192.0
56	55149.0
57	49106.0
58	44544.0
59	39982.0
60	36453.0
61	32924.0
62	29229.5
63	25535.0
64	20596.5
65	15658.0
66	11881.0
67	8104.0
68	6205.0
69	4306.0
70	3694.5
71	3083.0
72	2396.5
73	1710.0
74	1344.0
75	809.5
76	641.0
77	451.5
78	262.0
79	181.0
80	100.0
81	76.0
82	52.0
83	38.5
84	25.0
85	13.0
86	1.0
87	2.0
88	3.0
89	2.5
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1222285.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.378974136696723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.2256467979833	20.213107883494636
2	11.001039830946633	6.243964496784099
3	3.9067998300800064	3.3261291400527493
4	1.9151972185824064	2.1740532933129444
5	1.137348315673595	1.6138389217458262
6	0.8517614139784491	1.450326908275838
7	0.6302803439995605	1.2520696706862284
8	0.5512466619681926	1.2515051810348592
9	0.4863362735256839	1.2421552075310562
>10	7.995053994121918	52.25798325935715
>50	0.25255357990503857	4.3770931170647955
>100	0.040930057344797294	1.9763701382573398
>500	0.0031931250397161303	0.6093622570450766
>1k	0.0023222727561571857	1.4289573760583885
>5k	2.902840945196482E-4	0.5830831492990473
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7078	0.5790793472880711	No Hit
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	3121	0.25534143019017663	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC	2882	0.23578788907660653	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG	2711	0.2217976985727551	No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT	2091	0.17107303124884948	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	1870	0.15299214176726375	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC	1764	0.144319859934467	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCC	1582	0.12942971565551406	No Hit
TCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	1325	0.10840352290995962	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2725591821874603E-4	0.0	0.0	0.1765545678790135	0.0
2	3.2725591821874603E-4	0.0	0.0	0.781896202604139	0.0
3	3.2725591821874603E-4	0.0	0.0	1.0664452234953385	0.0
4	3.2725591821874603E-4	0.0	0.0	1.4988321054418567	0.0
5	3.2725591821874603E-4	0.0	0.0	2.7975472168929505	0.0
6	3.2725591821874603E-4	0.0	0.0	3.511128746568926	0.0
7	3.2725591821874603E-4	0.0	0.0	4.254981448680136	0.0
8	3.2725591821874603E-4	0.0	0.0	5.324126533500779	0.0
9	3.2725591821874603E-4	0.0	0.0	5.763058533811672	0.0
10	3.2725591821874603E-4	0.0	0.0	6.848484600563698	0.0
11	3.2725591821874603E-4	0.0	0.0	8.160780832620869	0.0
12	3.2725591821874603E-4	0.0	0.0	9.343892790961192	0.0
13	3.2725591821874603E-4	0.0	0.0	9.753044502714179	0.0
14	3.2725591821874603E-4	0.0	0.0	9.915363438150678	0.0
15	3.2725591821874603E-4	0.0	0.0	10.218320604441681	0.0
16	3.2725591821874603E-4	0.0	0.0	10.785782366632986	0.0
17	3.2725591821874603E-4	0.0	0.0	11.56686042944158	0.0
18	3.2725591821874603E-4	0.0	0.0	12.38459115509067	0.0
19	3.2725591821874603E-4	0.0	0.0	12.95892529156457	0.0
20	4.90883877328119E-4	0.0	0.0	13.48997983285404	0.0
21	4.90883877328119E-4	0.0	0.0	14.09262160625386	0.0
22	4.90883877328119E-4	0.0	0.0	14.838846913772157	0.0
23	4.90883877328119E-4	0.0	0.0	15.535329321721202	0.0
24	4.90883877328119E-4	0.0	0.0	16.062865861889822	0.0
25	4.90883877328119E-4	0.0	0.0	16.517915216172987	0.0
26	4.90883877328119E-4	0.0	0.0	16.957665356279428	0.0
27	5.726978568828055E-4	0.0	0.0	17.400115357711172	0.0
28	5.726978568828055E-4	0.0	0.0	17.860318992706283	0.0
29	5.726978568828055E-4	0.0	0.0	18.357829802378333	0.0
30	6.545118364374921E-4	0.0	0.0	18.9385454292575	0.0
31	6.545118364374921E-4	0.0	0.0	19.450210057392507	0.0
32	7.363258159921786E-4	0.0	0.0	19.93610328196779	0.0
33	7.363258159921786E-4	0.0	0.0	20.426578089398134	0.0
34	7.363258159921786E-4	0.0	0.0	20.908953312852567	0.0
35	7.363258159921786E-4	0.0	0.0	21.4202088710898	0.0
36	7.363258159921786E-4	0.0	0.0	21.91804693668007	0.0
37	7.363258159921786E-4	0.0	0.0	22.420384771145844	0.0
38	7.363258159921786E-4	0.0	0.0	22.948821265089567	0.0
39	7.363258159921786E-4	0.0	0.0	23.46703101158895	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	25	3.8909515E-5	45.0	24
CGATTCG	25	3.8909515E-5	45.0	10
TCGTGCG	50	2.1827873E-11	45.0	1
CGTTTTT	4000	0.0	41.456253	1
GCTAGCG	95	0.0	40.26316	1
ACGCCGC	45	1.9284926E-8	40.0	14
TCTAGCG	45	1.9284926E-8	40.0	1
AGTTGCG	45	1.9284926E-8	40.0	1
TATCGCG	45	1.9284926E-8	40.0	1
TCGGTCG	40	3.4584627E-7	39.375	1
TAACGCC	35	6.248614E-6	38.57143	12
CGCACGG	135	0.0	38.333332	2
CTAACGG	65	9.094947E-12	38.07692	2
CGAATAT	130	0.0	38.07692	14
TTTCGCG	30	1.13996444E-4	37.499996	1
CGCGATT	30	1.13996444E-4	37.499996	7
AATAGCG	60	1.5643309E-10	37.499996	1
TAGGGCG	280	0.0	36.964287	5
GCTACGA	135	0.0	36.666664	10
TATACGG	80	0.0	36.5625	2
>>END_MODULE