FastQCFastQC Report
Sat 18 Jun 2016
SRR3550622_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550622_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899057
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33710.3749484181759332No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC31780.3534814811519181No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG29530.3284552592327294No Hit
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC26460.2943083697696587TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC21180.23558016899929593TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC18520.2059936133081662No Hit
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT17210.19142279076854973TruSeq Adapter, Index 16 (95% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT14580.16216991803634254No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG13260.14748786784375184No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC12250.1362538748933605No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12210.1358089642814638No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA10430.11601044205206122No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCC9360.10410908318382484No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9250.10288557900110895No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT207.032466E-445.045
AGCGATT207.032466E-445.022
TCGTGTA1100.040.90909217
TACGAAT2500.040.49999612
AAATGCG451.927583E-840.01
CTACGAA2600.039.8076911
CGAATAT2500.039.614
GCTACGA2650.039.05660210
GACCCAC3600.038.7512
GGTTACG356.2467716E-638.5714261
GATTACG301.139724E-437.5000041
ATATCAC3850.036.81818430
TCTACGG800.036.56252
CGTTTTT20750.036.4337351
TATACTG2550.036.1764718
TGCGAGA250.00210686536.013
TCGCGCG250.00210686536.01
TTAACGG702.1827873E-1135.3571432
CGTCATA702.1827873E-1135.35714338
ATAAGGG4600.035.2173923
Produced by FastQC (version 0.11.5)