##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550622_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	899057
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23918839406178	33.0	31.0	34.0	30.0	34.0
2	32.36810569296496	34.0	31.0	34.0	30.0	34.0
3	32.34473676307509	34.0	31.0	34.0	30.0	34.0
4	35.924883516840424	37.0	35.0	37.0	35.0	37.0
5	36.02458353586035	37.0	35.0	37.0	35.0	37.0
6	36.06290924824566	37.0	35.0	37.0	35.0	37.0
7	36.11834955959411	37.0	36.0	37.0	35.0	37.0
8	36.08044095090745	37.0	36.0	37.0	35.0	37.0
9	37.88593381732193	39.0	38.0	39.0	35.0	39.0
10	37.62184933769494	39.0	37.0	39.0	35.0	39.0
11	37.549040828334576	39.0	37.0	39.0	35.0	39.0
12	37.481901592446306	39.0	37.0	39.0	35.0	39.0
13	37.4630529543733	39.0	37.0	39.0	35.0	39.0
14	38.738920891556376	40.0	38.0	41.0	35.0	41.0
15	38.81596050083588	40.0	38.0	41.0	35.0	41.0
16	38.875608554296335	40.0	38.0	41.0	35.0	41.0
17	38.83824162427966	40.0	38.0	41.0	35.0	41.0
18	38.751263824206916	40.0	38.0	41.0	35.0	41.0
19	38.69407835098331	40.0	38.0	41.0	35.0	41.0
20	38.5909870008242	40.0	38.0	41.0	35.0	41.0
21	38.51462365567478	40.0	38.0	41.0	34.0	41.0
22	38.51724640373191	40.0	38.0	41.0	35.0	41.0
23	38.489615230180064	40.0	38.0	41.0	35.0	41.0
24	38.41083490813152	40.0	37.0	41.0	34.0	41.0
25	38.33694637826078	40.0	37.0	41.0	34.0	41.0
26	38.356851679037035	40.0	37.0	41.0	34.0	41.0
27	38.305362173922234	40.0	37.0	41.0	34.0	41.0
28	38.24525030114887	40.0	37.0	41.0	34.0	41.0
29	38.20631839805485	40.0	37.0	41.0	34.0	41.0
30	38.118801143865184	40.0	37.0	41.0	34.0	41.0
31	38.08055996449613	40.0	37.0	41.0	34.0	41.0
32	37.942860130114106	40.0	37.0	41.0	34.0	41.0
33	37.8484378632278	40.0	37.0	41.0	34.0	41.0
34	37.77931543828701	40.0	37.0	41.0	33.0	41.0
35	37.70327465333121	40.0	36.0	41.0	33.0	41.0
36	37.63264398141608	40.0	36.0	41.0	33.0	41.0
37	37.58292522053663	40.0	36.0	41.0	33.0	41.0
38	37.52087687432499	40.0	36.0	41.0	33.0	41.0
39	37.42705857359433	40.0	36.0	41.0	33.0	41.0
40	37.33990948293601	40.0	35.0	41.0	33.0	41.0
41	37.24134621052948	40.0	35.0	41.0	33.0	41.0
42	37.19942117129392	40.0	35.0	41.0	33.0	41.0
43	37.110221042714755	40.0	35.0	41.0	33.0	41.0
44	37.03829123181289	39.0	35.0	41.0	32.0	41.0
45	36.959381885686895	39.0	35.0	41.0	32.0	41.0
46	36.85198602535768	39.0	35.0	41.0	32.0	41.0
47	36.7764057228852	39.0	35.0	41.0	31.0	41.0
48	36.714554249619326	39.0	35.0	41.0	31.0	41.0
49	36.63998611878891	39.0	35.0	41.0	31.0	41.0
50	36.53959648832054	39.0	35.0	41.0	31.0	41.0
51	35.585055230091086	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	16.0
9	29.0
10	38.0
11	21.0
12	33.0
13	20.0
14	23.0
15	34.0
16	49.0
17	107.0
18	166.0
19	264.0
20	526.0
21	889.0
22	1450.0
23	2080.0
24	3115.0
25	4259.0
26	5961.0
27	7129.0
28	7774.0
29	8899.0
30	10295.0
31	12953.0
32	16802.0
33	23036.0
34	45316.0
35	65555.0
36	58410.0
37	89810.0
38	162027.0
39	371490.0
40	477.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7565771691895	20.39881787250419	24.031513018640645	12.813091939665672
2	30.960884571278573	26.269413396480978	24.419363844561577	18.35033818767887
3	31.322151988138685	25.06114740222255	26.517784745572303	17.09891586406646
4	28.979920071808575	25.022328951334565	27.31728911515065	18.68046186170621
5	25.70738006600249	29.92279688607063	24.42848451210546	19.94133853582142
6	26.139165814848226	35.3094408919568	23.74754882059758	14.8038444725974
7	77.63334249107676	6.781550001835256	10.347174873228282	5.2379326338597
8	77.73344737875351	7.54390433532023	9.392952838362863	5.329695447563392
9	71.05967697265024	9.004100963565158	11.80125398055963	8.13496808322498
10	40.425245562850854	25.7460872892375	18.60182391105347	15.226843236858176
11	30.327999225855535	24.016274830183182	25.222983637299972	20.432742306661314
12	27.79289855926821	22.0696796754822	28.8549001898656	21.282521575383985
13	25.091512551484502	23.6609024789307	30.658679038147746	20.58890593143705
14	20.64140538364086	26.563944221556586	29.83170143828478	22.962948956517774
15	19.521787828802843	25.243338297794242	34.07492517159646	21.159948701806446
16	22.31204473131292	24.865497960640983	30.768460731633258	22.05399657641284
17	21.937429996095908	25.849862689462405	28.56926757702793	23.643439737413757
18	22.258655457885318	26.059081904706822	29.187582099911353	22.49468053749651
19	24.568853810158867	26.411451109328997	26.745134068251513	22.274561012260623
20	25.92060347675398	26.180876184713537	27.846843970960684	20.0516763675718
21	24.886853670012023	26.769715379558807	27.87187019287987	20.471560757549298
22	23.001767407405758	25.796028505422903	26.95335223461916	24.248851852552175
23	23.44111663665374	26.20278803234945	27.887775747255176	22.468319583741632
24	23.207761020713928	25.05847793855117	28.253158587275333	23.48060245345957
25	22.626040395659007	27.155230424767286	26.581963101338403	23.636766078235308
26	21.582947466067225	27.088938743594674	27.882548047565393	23.445565742772708
27	23.452906767869	26.041841618495827	28.186088312531908	22.319163301103266
28	20.806133537695608	26.73256534346543	28.796505672054163	23.664795446784797
29	22.192808687324607	24.652941915807343	28.985815137416203	24.16843425945185
30	22.054441487024736	25.417965712963692	28.491519447598986	24.036073352412586
31	22.546290168476528	25.571014963456157	26.125262358226454	25.757432509840868
32	22.65729536614475	25.905810198908412	26.85547190000189	24.581422534944945
33	21.952445729247422	25.99935265505969	26.400105888725633	25.648095726967256
34	20.098169526515004	25.67612509551675	27.364672095317648	26.8610332826506
35	21.490183603486766	24.162539193844218	28.848004075381205	25.499273127287815
36	20.856519664492907	27.67132673456744	27.023648111298836	24.448505489640812
37	20.624943691000684	27.62783672225454	27.989771505032497	23.757448081712283
38	20.462106407046495	26.945121388299075	26.317241287259876	26.27553091739456
39	22.138307137367264	24.620908351750778	27.28158503854594	25.959199472336014
40	21.761579076743747	24.005263292538736	29.703455954405563	24.52970167631196
41	19.640690189832235	26.523679811179935	28.64445747043847	25.19117252854936
42	20.44275279542899	26.448934828381294	29.11906586567926	23.989246510510455
43	22.44796492324736	25.382261636358983	28.093769360563346	24.07600407983031
44	21.319337928518436	24.99630168053861	27.306944943424057	26.3774154475189
45	21.767251687045427	23.901487892313835	26.875604105190217	27.455656315450522
46	21.673709230894147	25.937065169394153	27.290260795477927	25.09896480423377
47	20.462106407046495	25.16670244489504	30.42443360098414	23.946757547074323
48	20.804131439942072	24.341949398091558	28.790721834099507	26.063197327866867
49	20.971417830015227	24.455846514737107	29.72614639561229	24.846589259635373
50	20.00840881056485	24.119049181531317	29.35175411570123	26.52078789220261
51	19.958912504991343	23.39751539668786	28.03459624918109	28.60897584913971
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	402.0
1	1252.5
2	2103.0
3	4195.0
4	6287.0
5	4324.5
6	2362.0
7	2248.5
8	2135.0
9	2154.5
10	2174.0
11	2239.0
12	2304.0
13	2211.5
14	2119.0
15	2124.0
16	2129.0
17	2119.5
18	2110.0
19	2170.0
20	2230.0
21	2500.5
22	2771.0
23	3227.5
24	3684.0
25	4611.0
26	6838.5
27	8139.0
28	9770.5
29	11402.0
30	13782.5
31	16163.0
32	17170.0
33	18177.0
34	20569.0
35	22961.0
36	24630.0
37	26299.0
38	27887.5
39	29476.0
40	32980.5
41	36485.0
42	40613.0
43	44741.0
44	50115.0
45	55489.0
46	66440.0
47	77391.0
48	80142.5
49	82894.0
50	81187.5
51	79481.0
52	70937.5
53	62394.0
54	56323.0
55	50252.0
56	47614.5
57	44977.0
58	43140.5
59	41304.0
60	41349.5
61	41395.0
62	37712.5
63	34030.0
64	30382.0
65	26734.0
66	22496.5
67	18259.0
68	15468.0
69	12677.0
70	10272.5
71	7868.0
72	6602.0
73	5336.0
74	4181.5
75	2423.5
76	1820.0
77	1337.5
78	855.0
79	632.0
80	409.0
81	287.0
82	165.0
83	106.5
84	48.0
85	38.0
86	28.0
87	20.0
88	12.0
89	11.5
90	11.0
91	8.5
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	2.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	899057.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.258078370558465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.499548166984	19.489402873951697
2	9.714664864488268	5.296061924398638
3	3.5234401998071005	2.881266273009544
4	1.8468269583454346	2.013638158697599
5	1.23745875977639	1.686537392715946
6	0.9032320373950062	1.4772221797267364
7	0.7722907944303962	1.4735814099611217
8	0.6829346021434634	1.4892387925754151
9	0.586888333170863	1.439770336230403
>10	9.005470057203796	52.96398472276199
>50	0.15615404393159424	2.791005074478976
>100	0.060406958390679305	3.174870453249596
>500	0.005753043656255173	1.1225850861722921
>1k	0.0049311802767901475	2.700835322070077
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3371	0.3749484181759332	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	3178	0.3534814811519181	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG	2953	0.3284552592327294	No Hit
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	2646	0.2943083697696587	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	2118	0.23558016899929593	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC	1852	0.2059936133081662	No Hit
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT	1721	0.19142279076854973	TruSeq Adapter, Index 16 (95% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1458	0.16216991803634254	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1326	0.14748786784375184	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1225	0.1362538748933605	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1221	0.1358089642814638	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1043	0.11601044205206122	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCC	936	0.10410908318382484	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	925	0.10288557900110895	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19676171811130996	0.0
2	0.0	0.0	0.0	0.9149586733655375	0.0
3	0.0	0.0	0.0	1.2461946239226211	0.0
4	0.0	0.0	0.0	1.7194682873277223	0.0
5	0.0	0.0	0.0	3.2468464179690497	0.0
6	0.0	0.0	0.0	4.091509214654911	0.0
7	0.0	0.0	0.0	4.9588624525475025	0.0
8	0.0	0.0	0.0	6.193378172907836	0.0
9	0.0	0.0	0.0	6.673103040185438	0.0
10	0.0	0.0	0.0	7.929308152875735	0.0
11	1.1122765297417183E-4	0.0	0.0	9.234675888180616	0.0
12	1.1122765297417183E-4	0.0	0.0	10.43137420652973	0.0
13	1.1122765297417183E-4	0.0	0.0	10.863048727722491	0.0
14	1.1122765297417183E-4	0.0	0.0	11.05391538022617	0.0
15	1.1122765297417183E-4	0.0	0.0	11.400611974546663	0.0
16	1.1122765297417183E-4	0.0	0.0	11.97376807032257	0.0
17	1.1122765297417183E-4	0.0	0.0	12.588856991269742	0.0
18	1.1122765297417183E-4	0.0	0.0	13.239427533515672	0.0
19	1.1122765297417183E-4	0.0	0.0	13.701244748664434	0.0
20	1.1122765297417183E-4	0.0	0.0	14.110006373344515	0.0
21	1.1122765297417183E-4	0.0	0.0	14.652463636899551	0.0
22	1.1122765297417183E-4	0.0	0.0	15.254094011836846	0.0
23	1.1122765297417183E-4	0.0	0.0	15.780868176322524	0.0
24	1.1122765297417183E-4	0.0	0.0	16.2443538062659	0.0
25	1.1122765297417183E-4	0.0	0.0	16.646775454726452	0.0
26	1.1122765297417183E-4	0.0	0.0	17.02906489799868	0.0
27	1.1122765297417183E-4	0.0	0.0	17.42147605769156	0.0
28	2.2245530594834366E-4	0.0	0.0	17.81689036401474	0.0
29	2.2245530594834366E-4	0.0	0.0	18.233994062667882	0.0
30	2.2245530594834366E-4	0.0	0.0	18.68813656976143	0.0
31	2.2245530594834366E-4	0.0	0.0	19.083662103737584	0.0
32	2.2245530594834366E-4	0.0	0.0	19.50510368085672	0.0
33	2.2245530594834366E-4	0.0	0.0	19.894178010960374	0.0
34	2.2245530594834366E-4	0.0	0.0	20.263120135875702	0.0
35	2.2245530594834366E-4	0.0	0.0	20.654641474344785	0.0
36	2.2245530594834366E-4	0.0	0.0	21.042269844959776	0.0
37	3.3368295892251547E-4	0.0	0.0	21.428897166697997	0.0
38	3.3368295892251547E-4	0.0	0.0	21.81374484598863	0.0
39	3.3368295892251547E-4	0.0	0.0	22.200928305991724	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTACGT	20	7.032466E-4	45.0	45
AGCGATT	20	7.032466E-4	45.0	22
TCGTGTA	110	0.0	40.909092	17
TACGAAT	250	0.0	40.499996	12
AAATGCG	45	1.927583E-8	40.0	1
CTACGAA	260	0.0	39.80769	11
CGAATAT	250	0.0	39.6	14
GCTACGA	265	0.0	39.056602	10
GACCCAC	360	0.0	38.75	12
GGTTACG	35	6.2467716E-6	38.571426	1
GATTACG	30	1.139724E-4	37.500004	1
ATATCAC	385	0.0	36.818184	30
TCTACGG	80	0.0	36.5625	2
CGTTTTT	2075	0.0	36.433735	1
TATACTG	255	0.0	36.17647	18
TGCGAGA	25	0.002106865	36.0	13
TCGCGCG	25	0.002106865	36.0	1
TTAACGG	70	2.1827873E-11	35.357143	2
CGTCATA	70	2.1827873E-11	35.357143	38
ATAAGGG	460	0.0	35.217392	3
>>END_MODULE