SRR3550620_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550620_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	619223
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1653	0.26694744865743036	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1649	0.2663014778197838	No Hit
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	1628	0.2629101309221395	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	1457	0.23529487761275017	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	1449	0.23400293593745708	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCC	875	0.14130612073517942	No Hit
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	713	0.11514430181049477	TruSeq Adapter, Index 27 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAT	25	3.888715E-5	45.0	9
CGCATCG	20	7.030763E-4	45.0	21
CGCTCAT	20	7.030763E-4	45.0	24
ATAACGC	25	3.888715E-5	45.0	11
TCGCGCA	20	7.030763E-4	45.0	34
GCTACGA	235	0.0	43.085106	10
CGAATAT	245	0.0	42.2449	14
CTACGAA	250	0.0	41.399998	11
CGTATGG	60	3.6379788E-12	41.250004	2
TACGAAT	255	0.0	40.588234	12
TTTAGCG	50	1.0804797E-9	40.5	1
AAGCTAC	255	0.0	39.705883	8
CTCGATC	80	0.0	39.375	16
GCGTATG	40	3.4547702E-7	39.375	1
TAGTCCG	40	3.4547702E-7	39.375	1
TCACGAC	35	6.243621E-6	38.57143	25
TCGTTCA	35	6.243621E-6	38.57143	16
GCCGATA	35	6.243621E-6	38.57143	9
CGGTCTA	35	6.243621E-6	38.57143	31
ATTGCGG	70	0.0	38.57143	2