##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550620_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	619223
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24205657735582	33.0	31.0	34.0	30.0	34.0
2	32.36665466237527	34.0	31.0	34.0	30.0	34.0
3	32.33462258346347	34.0	31.0	34.0	30.0	34.0
4	35.913871093289494	37.0	35.0	37.0	35.0	37.0
5	36.01960521492257	37.0	35.0	37.0	35.0	37.0
6	36.053702785587745	37.0	35.0	37.0	35.0	37.0
7	36.13783402748283	37.0	36.0	37.0	35.0	37.0
8	36.114259321762916	37.0	37.0	37.0	35.0	37.0
9	37.90989191292959	39.0	38.0	39.0	35.0	39.0
10	37.62815011716296	39.0	37.0	39.0	35.0	39.0
11	37.55122952474311	39.0	37.0	39.0	35.0	39.0
12	37.43750797370253	39.0	37.0	39.0	35.0	39.0
13	37.398780407058524	39.0	37.0	39.0	35.0	39.0
14	38.6231971357653	40.0	38.0	41.0	35.0	41.0
15	38.744491079950194	40.0	38.0	41.0	35.0	41.0
16	38.800613026324925	40.0	38.0	41.0	35.0	41.0
17	38.780830169422	40.0	38.0	41.0	35.0	41.0
18	38.71518176811908	40.0	38.0	41.0	35.0	41.0
19	38.65159401378825	40.0	38.0	41.0	35.0	41.0
20	38.57306010920137	40.0	38.0	41.0	35.0	41.0
21	38.49295488055192	40.0	37.0	41.0	34.0	41.0
22	38.49931446344855	40.0	37.0	41.0	35.0	41.0
23	38.4668011362627	40.0	37.0	41.0	35.0	41.0
24	38.38520371497829	40.0	37.0	41.0	34.0	41.0
25	38.301455210804505	40.0	37.0	41.0	34.0	41.0
26	38.322912747104034	40.0	37.0	41.0	34.0	41.0
27	38.27763019138501	40.0	37.0	41.0	34.0	41.0
28	38.22603650058218	40.0	37.0	41.0	34.0	41.0
29	38.182748379824396	40.0	37.0	41.0	34.0	41.0
30	38.08127120601141	40.0	36.0	41.0	34.0	41.0
31	38.036373002940785	40.0	36.0	41.0	34.0	41.0
32	37.89181603396515	40.0	36.0	41.0	34.0	41.0
33	37.80057911285595	40.0	36.0	41.0	34.0	41.0
34	37.746055944304395	40.0	36.0	41.0	33.0	41.0
35	37.677752602858746	40.0	36.0	41.0	33.0	41.0
36	37.61088654652686	40.0	36.0	41.0	33.0	41.0
37	37.568489865525024	40.0	36.0	41.0	33.0	41.0
38	37.48774189589211	40.0	35.0	41.0	33.0	41.0
39	37.4169031189087	40.0	35.0	41.0	33.0	41.0
40	37.32009146947061	40.0	35.0	41.0	33.0	41.0
41	37.23911095033615	40.0	35.0	41.0	33.0	41.0
42	37.220980163850506	40.0	35.0	41.0	33.0	41.0
43	37.146977744689714	40.0	35.0	41.0	33.0	41.0
44	37.068652165697976	39.0	35.0	41.0	33.0	41.0
45	36.978101588603785	39.0	35.0	41.0	32.0	41.0
46	36.872186595136164	39.0	35.0	41.0	32.0	41.0
47	36.80994891985601	39.0	35.0	41.0	32.0	41.0
48	36.74880939499986	39.0	35.0	41.0	32.0	41.0
49	36.68723706968249	39.0	35.0	41.0	32.0	41.0
50	36.5906192114957	39.0	35.0	41.0	31.0	41.0
51	35.62044852985112	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	17.0
10	27.0
11	20.0
12	14.0
13	14.0
14	21.0
15	21.0
16	51.0
17	72.0
18	113.0
19	202.0
20	351.0
21	548.0
22	867.0
23	1340.0
24	1964.0
25	2967.0
26	4153.0
27	4737.0
28	5196.0
29	5908.0
30	7078.0
31	8959.0
32	11768.0
33	16436.0
34	33400.0
35	48632.0
36	39499.0
37	59000.0
38	109868.0
39	255630.0
40	340.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.80836306144959	21.782136645441142	24.61439578310237	11.795104510006896
2	32.61910491050882	25.30235472519593	24.53784823884126	17.540692125453997
3	32.224255235997376	23.646085497470217	27.526270826503534	16.603388440028873
4	29.491314114624295	25.872101004000175	25.37857928403822	19.25800559733731
5	25.032177422350266	30.34803293805301	24.472282198820132	20.147507440776586
6	27.218950200493197	33.00038919742968	24.45887830393897	15.321782298138151
7	78.96282922307472	6.910596021142626	8.946696101404502	5.179878654378148
8	79.36058576635557	6.753786600303929	8.91294412513747	4.972683508203022
9	72.68383118844099	8.971565978653894	11.407360514709563	6.937242318195545
10	40.35638211112959	26.886436711814643	17.974461542933646	14.782719634122119
11	31.309076051761643	25.206912533933657	24.468406373794256	19.015605040510447
12	30.232727143533104	22.452654374918243	28.1323529649254	19.18226551662325
13	25.52440720063693	24.93415134773741	28.668024282043785	20.873417169581877
14	20.36455364222582	27.908201084262053	28.686757436335537	23.04048783717659
15	19.606829849666436	26.6734278281007	33.51393601335867	20.205806308874187
16	21.71107985330002	25.918610904310725	31.78612551536361	20.584183727025643
17	21.910846334842212	25.339982526488843	28.289969849311152	24.459201289357793
18	22.80616191582031	26.02777997587299	29.033643776151724	22.132414332154976
19	24.769751769556365	27.019668197079245	26.961207836272234	21.24937219709216
20	27.39239337040129	25.437362630264055	27.55001025478705	19.6202337445476
21	25.02119591811028	26.714446976291256	27.51012155556237	20.754235550036093
22	22.887069763235537	25.889057738488397	26.923095556851084	24.300776941424978
23	23.380107005069256	26.256292159690453	28.082451717717205	22.28114911752309
24	23.707291234337227	24.91477222260801	28.843082379046	22.534854164008767
25	22.855255699481447	27.032103135703938	26.72930430555713	23.38333685925749
26	21.54942565117898	28.423847305413396	27.123830994649744	22.902896048757878
27	22.393225057854764	27.804845750238606	27.921281993724396	21.88064719818224
28	21.114202799314626	28.066786924904275	28.17369509853478	22.64531517724632
29	22.025506158524475	26.630309274687797	28.708558952106106	22.635625614681626
30	22.556332694360513	26.193794481148146	28.131061023250105	23.118811801241232
31	23.48798413495623	27.99185430773728	26.33025582060098	22.18990573670552
32	24.26621750161089	28.360057685195798	25.841255896502553	21.532468916690757
33	23.98150585491818	27.178738515849705	26.296826829752774	22.54292879947935
34	22.258863123624284	25.990313667289488	29.126663576772827	22.624159632313397
35	22.63126531152751	25.743714300017928	27.470878827175348	24.154141561279214
36	21.777614849577613	27.224440952613193	27.02418999294277	23.97375420486642
37	23.310826632731665	28.187744964253586	27.39303934123894	21.108389061775807
38	23.142712722234155	27.34895183156956	26.470108506951455	23.038226939244826
39	22.9545737157696	25.935567638798947	28.472779596365122	22.63707904906633
40	23.835516445610065	24.274453629790884	30.74966530635975	21.14036461823931
41	20.801552913893705	25.544916774732208	29.292193603919753	24.361336707454342
42	22.517251458682896	25.528928996500454	27.624619886535225	24.32919965828143
43	22.75480723422741	25.490655224369895	27.61638375835523	24.138153783047464
44	22.124016711265572	25.80540451501317	27.256255016367287	24.814323757353975
45	22.321683787585407	25.160402633623104	27.325535388704875	25.192378190086608
46	21.496456042491964	26.660992889476002	28.014786272473728	23.827764795558302
47	21.627426629824797	25.479673720129902	30.072364883087353	22.820534766957945
48	21.640184553868316	24.423349907868406	29.661042952215922	24.275422586047352
49	21.56347551689779	24.739391140186974	29.459661543579614	24.23747179933562
50	20.231160664251814	24.848237226330415	29.563501355731297	25.357100753686474
51	20.216626320404764	24.27413064437206	28.520419945641557	26.988823089581622
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	480.0
1	939.5
2	1399.0
3	2412.0
4	3425.0
5	2467.5
6	1510.0
7	1500.0
8	1490.0
9	1540.0
10	1590.0
11	1516.0
12	1442.0
13	1554.5
14	1667.0
15	1661.5
16	1656.0
17	1778.5
18	1901.0
19	1899.0
20	1897.0
21	1979.5
22	2062.0
23	2513.0
24	2964.0
25	3461.0
26	4754.5
27	5551.0
28	6915.5
29	8280.0
30	9473.5
31	10667.0
32	11713.5
33	12760.0
34	14933.0
35	17106.0
36	17519.5
37	17933.0
38	19941.5
39	21950.0
40	24708.0
41	27466.0
42	30775.5
43	34085.0
44	38044.5
45	42004.0
46	45460.5
47	48917.0
48	52372.5
49	55828.0
50	55217.0
51	54606.0
52	47831.0
53	41056.0
54	37422.5
55	33789.0
56	31864.0
57	29939.0
58	29671.5
59	29404.0
60	29412.0
61	29420.0
62	27390.0
63	25360.0
64	21087.5
65	16815.0
66	13587.0
67	10359.0
68	8933.5
69	7508.0
70	5904.0
71	4300.0
72	3646.5
73	2993.0
74	2400.5
75	1402.5
76	997.0
77	737.0
78	477.0
79	361.5
80	246.0
81	187.0
82	128.0
83	71.0
84	14.0
85	9.0
86	4.0
87	4.5
88	5.0
89	5.0
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	619223.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.519068186672786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.81875836136147	21.495418933378787
2	9.51770892490193	5.619077974701684
3	3.344350409831963	2.9616632336387037
4	1.6932305415859334	1.9993035125132823
5	1.0810484811798082	1.5955771914522905
6	0.8498812804873012	1.5052622081568852
7	0.7218625667972152	1.4916097231485543
8	0.5929442848653931	1.4002530220671576
9	0.582492883785323	1.5475182439239554
>10	8.618424519446112	53.600107382943584
>50	0.12227338625054711	2.3229622200913265
>100	0.05208955916502893	2.7598124870941865
>500	0.0021932445964222705	0.43312757113741385
>1k	0.002741555745527838	1.268306295752158
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1653	0.26694744865743036	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1649	0.2663014778197838	No Hit
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	1628	0.2629101309221395	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	1457	0.23529487761275017	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	1449	0.23400293593745708	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCC	875	0.14130612073517942	No Hit
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	713	0.11514430181049477	TruSeq Adapter, Index 27 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.229854188232672E-4	0.0	0.0	0.2454689183056831	0.0
2	3.229854188232672E-4	0.0	0.0	0.957328781392164	0.0
3	3.229854188232672E-4	0.0	0.0	1.3339297797400935	0.0
4	3.229854188232672E-4	0.0	0.0	1.784655931707963	0.0
5	3.229854188232672E-4	0.0	0.0	2.99633573042345	0.0
6	3.229854188232672E-4	0.0	0.0	3.649089261865273	0.0
7	3.229854188232672E-4	0.0	0.0	4.258885732603602	0.0
8	3.229854188232672E-4	0.0	0.0	5.08120660892764	0.0
9	3.229854188232672E-4	0.0	0.0	5.4553852166343955	0.0
10	3.229854188232672E-4	0.0	0.0	6.307905229618409	0.0
11	3.229854188232672E-4	0.0	0.0	7.644095907290265	0.0
12	3.229854188232672E-4	0.0	0.0	8.642282344163572	0.0
13	3.229854188232672E-4	0.0	0.0	9.056511143804412	0.0
14	3.229854188232672E-4	0.0	0.0	9.23415312415721	0.0
15	3.229854188232672E-4	0.0	0.0	9.562467802391062	0.0
16	3.229854188232672E-4	0.0	0.0	10.205370278558775	0.0
17	3.229854188232672E-4	0.0	0.0	10.918199097901725	0.0
18	4.844781282349008E-4	0.0	0.0	11.705314563574028	0.0
19	4.844781282349008E-4	0.0	0.0	12.20077419604892	0.0
20	4.844781282349008E-4	0.0	0.0	12.68832068576264	0.0
21	4.844781282349008E-4	0.0	0.0	13.270178917772757	0.0
22	4.844781282349008E-4	0.0	0.0	13.948448297301619	0.0
23	4.844781282349008E-4	0.0	0.0	14.573102097305817	0.0
24	4.844781282349008E-4	0.0	0.0	15.0420769254372	0.0
25	4.844781282349008E-4	0.0	0.0	15.454529305274514	0.0
26	4.844781282349008E-4	0.0	0.0	15.841788822443611	0.0
27	6.459708376465344E-4	0.0	0.0	16.221135196851538	0.0
28	6.459708376465344E-4	0.0	0.0	16.636171460039435	0.0
29	6.459708376465344E-4	0.0	0.0	17.06267370559556	0.0
30	6.459708376465344E-4	0.0	0.0	17.525511810769302	0.0
31	6.459708376465344E-4	0.0	0.0	17.958635257411306	0.0
32	6.459708376465344E-4	0.0	0.0	18.401771252036827	0.0
33	6.459708376465344E-4	0.0	0.0	18.82229826734472	0.0
34	6.459708376465344E-4	0.0	0.0	19.228129446096155	0.0
35	6.459708376465344E-4	0.0	0.0	19.650271388498165	0.0
36	6.459708376465344E-4	0.0	0.0	20.08856260184134	0.0
37	6.459708376465344E-4	0.0	0.0	20.53815830484333	0.0
38	6.459708376465344E-4	0.0	0.0	20.959331290988867	0.0
39	6.459708376465344E-4	0.0	0.0	21.415548195076735	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAT	25	3.888715E-5	45.0	9
CGCATCG	20	7.030763E-4	45.0	21
CGCTCAT	20	7.030763E-4	45.0	24
ATAACGC	25	3.888715E-5	45.0	11
TCGCGCA	20	7.030763E-4	45.0	34
GCTACGA	235	0.0	43.085106	10
CGAATAT	245	0.0	42.2449	14
CTACGAA	250	0.0	41.399998	11
CGTATGG	60	3.6379788E-12	41.250004	2
TACGAAT	255	0.0	40.588234	12
TTTAGCG	50	1.0804797E-9	40.5	1
AAGCTAC	255	0.0	39.705883	8
CTCGATC	80	0.0	39.375	16
GCGTATG	40	3.4547702E-7	39.375	1
TAGTCCG	40	3.4547702E-7	39.375	1
TCACGAC	35	6.243621E-6	38.57143	25
TCGTTCA	35	6.243621E-6	38.57143	16
GCCGATA	35	6.243621E-6	38.57143	9
CGGTCTA	35	6.243621E-6	38.57143	31
ATTGCGG	70	0.0	38.57143	2
>>END_MODULE