FastQCFastQC Report
Sat 18 Jun 2016
SRR3550617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426400
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12560.29455909943714825No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT10680.25046904315197No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC8780.20590994371482177No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCC7580.17776735459662288No Hit
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT7170.16815196998123827No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6980.16369606003752346No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA6530.1531425891181989No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6390.149859287054409No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6310.1479831144465291No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6220.14587242026266417No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA5010.1174953095684803No Hit
CCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC4990.11702626641651032No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC207.0282904E-445.06
ACCGGGT207.0282904E-445.04
TTGTCGC406.7975634E-945.038
ATTCGCG207.0282904E-445.01
TACGCGG207.0282904E-445.02
TTACGCG207.0282904E-445.01
TGTCGCA351.2095188E-745.039
TGCAACG253.8866645E-545.01
AGTATCA302.1622782E-644.99999632
CGTTAGG302.1622782E-644.9999962
GCTTTCG302.1622782E-644.99999610
CGGGTCA556.002665E-1140.9090926
ATTGCGG501.0786607E-940.52
TGCTACG403.451405E-739.3751
GACGAGT356.23905E-638.57142644
AATACGG356.23905E-638.5714262
TAGGGAC2050.038.4146355
CTACGAA1250.037.80000311
TACGAAT1250.037.80000312
GCTACGA1250.037.80000310
Produced by FastQC (version 0.11.5)