##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550617_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	426400
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30762429643527	33.0	31.0	34.0	30.0	34.0
2	32.429971857410884	34.0	31.0	34.0	31.0	34.0
3	32.40341228893058	34.0	31.0	34.0	30.0	34.0
4	35.96269230769231	37.0	35.0	37.0	35.0	37.0
5	36.06000703564728	37.0	35.0	37.0	35.0	37.0
6	36.094242495309565	37.0	36.0	37.0	35.0	37.0
7	36.15958020637899	37.0	37.0	37.0	35.0	37.0
8	36.13475140712946	37.0	37.0	37.0	35.0	37.0
9	37.95664868667917	39.0	38.0	39.0	35.0	39.0
10	37.65842166979362	39.0	37.0	39.0	35.0	39.0
11	37.57598264540338	39.0	37.0	39.0	35.0	39.0
12	37.47106707317073	39.0	37.0	39.0	35.0	39.0
13	37.426435272045026	39.0	37.0	39.0	35.0	39.0
14	38.69828095684803	40.0	38.0	41.0	35.0	41.0
15	38.835234521575984	40.0	38.0	41.0	35.0	41.0
16	38.88188320825516	40.0	38.0	41.0	35.0	41.0
17	38.84591463414634	40.0	38.0	41.0	35.0	41.0
18	38.77812148217636	40.0	38.0	41.0	35.0	41.0
19	38.71601547842401	40.0	38.0	41.0	35.0	41.0
20	38.628445121951216	40.0	38.0	41.0	35.0	41.0
21	38.53996013133208	40.0	38.0	41.0	35.0	41.0
22	38.55004221388368	40.0	38.0	41.0	35.0	41.0
23	38.50712242026266	40.0	37.0	41.0	35.0	41.0
24	38.41604596622889	40.0	37.0	41.0	35.0	41.0
25	38.317108348968105	40.0	37.0	41.0	34.0	41.0
26	38.34025093808631	40.0	37.0	41.0	34.0	41.0
27	38.30022983114446	40.0	37.0	41.0	34.0	41.0
28	38.25310272045028	40.0	37.0	41.0	34.0	41.0
29	38.19070825515947	40.0	37.0	41.0	34.0	41.0
30	38.08736632270169	40.0	36.0	41.0	34.0	41.0
31	38.02463414634146	40.0	36.0	41.0	34.0	41.0
32	37.86507035647279	40.0	36.0	41.0	34.0	41.0
33	37.7562148217636	40.0	36.0	41.0	34.0	41.0
34	37.66897983114446	40.0	36.0	41.0	33.0	41.0
35	37.587706378986866	40.0	36.0	41.0	33.0	41.0
36	37.509500469043154	40.0	36.0	41.0	33.0	41.0
37	37.4125703564728	40.0	35.0	41.0	33.0	41.0
38	37.34762664165103	40.0	35.0	41.0	33.0	41.0
39	37.27772983114446	40.0	35.0	41.0	33.0	41.0
40	37.196536116322704	40.0	35.0	41.0	33.0	41.0
41	37.12809803001876	40.0	35.0	41.0	33.0	41.0
42	37.09631801125703	40.0	35.0	41.0	33.0	41.0
43	37.00566135084428	40.0	35.0	41.0	32.0	41.0
44	36.90216228893058	39.0	35.0	41.0	32.0	41.0
45	36.8225234521576	39.0	35.0	41.0	32.0	41.0
46	36.719444183864915	39.0	35.0	41.0	31.0	41.0
47	36.6868808630394	39.0	35.0	41.0	31.0	41.0
48	36.60709896810506	39.0	35.0	41.0	31.0	41.0
49	36.54218808630394	39.0	35.0	41.0	31.0	41.0
50	36.461036585365854	39.0	35.0	41.0	31.0	41.0
51	35.555548780487804	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	14.0
10	21.0
11	18.0
12	17.0
13	23.0
14	13.0
15	28.0
16	28.0
17	56.0
18	98.0
19	170.0
20	272.0
21	392.0
22	609.0
23	935.0
24	1441.0
25	2321.0
26	3053.0
27	3820.0
28	3856.0
29	4174.0
30	4825.0
31	5944.0
32	7523.0
33	10772.0
34	22766.0
35	33413.0
36	26786.0
37	40821.0
38	75703.0
39	176215.0
40	269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.30816135084427	21.18996247654784	24.652673545966227	11.84920262664165
2	32.69770168855535	25.337007504690433	25.131097560975608	16.83419324577861
3	33.27321763602251	23.540337711069416	26.596153846153847	16.590290806754222
4	29.570356472795496	25.690196998123827	26.356941838649156	18.38250469043152
5	25.731472795497183	31.07129455909944	24.239446529080674	18.9577861163227
6	26.415103189493433	35.585131332082554	23.733348968105066	14.26641651031895
7	78.92002814258912	7.405253283302065	8.957786116322701	4.7169324577861165
8	78.61444652908067	7.775093808630394	9.027204502814259	4.583255159474672
9	71.94465290806754	10.449812382739212	11.306988742964352	6.298545966228892
10	40.38696060037523	27.57621951219512	18.400797373358348	13.636022514071295
11	30.790572232645403	26.151969981238278	24.374765478424017	18.68269230769231
12	29.74906191369606	23.899155722326455	27.490853658536587	18.8609287054409
13	25.04268292682927	26.582786116322705	28.17354596622889	20.200984990619137
14	19.97795497185741	29.225844277673545	29.102720450281428	21.693480300187616
15	20.66627579737336	27.238039399624764	32.245544090056285	19.850140712945592
16	22.275093808630395	26.575046904315197	31.098968105065666	20.050891181988742
17	22.780253283302063	26.71810506566604	28.081613508442775	22.420028142589118
18	22.673076923076923	27.59169793621013	28.38391181988743	21.351313320825515
19	25.096388367729833	27.143996247654783	26.77016885553471	20.989446529080674
20	25.545966228893057	27.63391181988743	27.971153846153847	18.848968105065666
21	24.753986866791745	27.70544090056285	27.705909943714822	19.83466228893058
22	23.49718574108818	26.396810506566602	27.160178236397748	22.94582551594747
23	23.75656660412758	26.48030018761726	28.45075046904315	21.31238273921201
24	23.136022514071293	26.530018761726076	28.654549718574106	21.679409005628518
25	22.257973733583487	28.36421200750469	27.553939962476548	21.82387429643527
26	21.375703564727957	30.238273921200747	26.921904315196997	21.464118198874296
27	22.625	28.133208255159474	28.886022514071296	20.35576923076923
28	20.805347091932457	28.498358348968107	29.063320825515948	21.63297373358349
29	21.6156191369606	27.757035647279547	28.907598499061915	21.719746716697934
30	21.532129455909942	29.52673545966229	26.95966228893058	21.981472795497186
31	24.11608818011257	28.938086303939965	25.46669793621013	21.479127579737337
32	23.073170731707318	29.59803001876173	26.039634146341463	21.289165103189493
33	22.371951219512194	29.88625703564728	25.90126641651032	21.84052532833021
34	21.039165103189493	28.986397748592868	26.919793621013135	23.0546435272045
35	22.862570356472794	27.95262664165103	26.193714821763603	22.99108818011257
36	22.55300187617261	30.544090056285178	25.400562851782365	21.50234521575985
37	23.245544090056285	29.597560975609756	26.776266416510317	20.38062851782364
38	21.9359756097561	28.70239212007505	26.578095684803	22.783536585365855
39	23.02767354596623	27.24765478424015	27.489915572232643	22.234756097560975
40	22.772279549718576	27.460834896810503	27.450046904315194	22.316838649155724
41	20.72701688555347	27.233348968105066	27.45075046904315	24.588883677298313
42	21.787523452157597	27.552298311444652	27.185975609756095	23.474202626641652
43	23.78048780487805	26.842166979362105	27.62359287054409	21.75375234521576
44	22.034474671669795	28.10530018761726	26.625703564727953	23.23452157598499
45	21.82715759849906	28.33231707317073	25.862804878048777	23.977720450281424
46	21.515712945590995	29.21904315196998	25.99155722326454	23.273686679174485
47	22.402439024390244	26.829033771106943	28.532129455909942	22.23639774859287
48	21.645872420262663	26.702392120075046	28.114446529080677	23.537288930581614
49	21.484287054409005	27.03517823639775	28.634146341463413	22.84638836772983
50	20.10389305816135	26.939024390243905	29.060741088180116	23.896341463414632
51	20.359990619136962	26.618198874296432	27.288227016885553	25.73358348968105
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	421.0
1	852.5
2	1284.0
3	2095.5
4	2907.0
5	2140.0
6	1373.0
7	1322.0
8	1271.0
9	1277.0
10	1283.0
11	1354.0
12	1425.0
13	1393.0
14	1361.0
15	1399.0
16	1437.0
17	1329.5
18	1222.0
19	1222.5
20	1223.0
21	1468.0
22	1713.0
23	1961.5
24	2210.0
25	2901.5
26	4383.5
27	5174.0
28	5931.0
29	6688.0
30	7771.5
31	8855.0
32	10367.5
33	11880.0
34	12336.0
35	12792.0
36	13551.5
37	14311.0
38	15342.0
39	16373.0
40	18705.0
41	21037.0
42	23179.5
43	25322.0
44	26926.0
45	28530.0
46	30451.0
47	32372.0
48	33669.5
49	34967.0
50	34256.0
51	33545.0
52	29941.5
53	26338.0
54	24263.5
55	22189.0
56	21262.0
57	20335.0
58	19263.0
59	18191.0
60	17879.0
61	17567.0
62	16167.0
63	14767.0
64	12624.0
65	10481.0
66	8890.5
67	7300.0
68	6265.0
69	5230.0
70	4717.0
71	4204.0
72	3254.0
73	2304.0
74	1857.0
75	1028.0
76	646.0
77	554.5
78	463.0
79	339.0
80	215.0
81	158.0
82	101.0
83	86.0
84	71.0
85	39.0
86	7.0
87	6.5
88	6.0
89	4.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	426400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.21575443297421
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.37923847564743	20.70450573307521
2	8.531202207384723	4.814286130032297
3	2.921491021493746	2.4729621972181954
4	1.5676974642766826	1.7693506670890893
5	1.04149879204229	1.4693337079252264
6	0.8811570061555826	1.49175058215484
7	0.7245139258708009	1.4309894910948417
8	0.6595439888094674	1.4887624980793779
9	0.6029137354436687	1.531049931319116
>10	9.516698068459686	56.421561216122655
>50	0.12491290508319436	2.2824738775161295
>100	0.039972129626622194	2.144313012786926
>500	0.007494774304991661	1.4325965854659093
>1k	0.001665505401109258	0.5460643701201859
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1256	0.29455909943714825	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1068	0.25046904315197	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	878	0.20590994371482177	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCC	758	0.17776735459662288	No Hit
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT	717	0.16815196998123827	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	698	0.16369606003752346	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	653	0.1531425891181989	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	639	0.149859287054409	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	631	0.1479831144465291	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	622	0.14587242026266417	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	501	0.1174953095684803	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	499	0.11702626641651032	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.035647279549719E-4	0.0	0.0	0.174718574108818	0.0
2	7.035647279549719E-4	0.0	0.0	0.650328330206379	0.0
3	7.035647279549719E-4	0.0	0.0	0.8878986866791745	0.0
4	7.035647279549719E-4	0.0	0.0	1.202861163227017	0.0
5	7.035647279549719E-4	0.0	0.0	2.050656660412758	0.0
6	7.035647279549719E-4	0.0	0.0	2.50234521575985	0.0
7	7.035647279549719E-4	0.0	0.0	2.9685741088180113	0.0
8	7.035647279549719E-4	0.0	0.0	3.648921200750469	0.0
9	7.035647279549719E-4	0.0	0.0	3.9392589118198873	0.0
10	7.035647279549719E-4	0.0	0.0	4.570356472795497	0.0
11	7.035647279549719E-4	0.0	0.0	5.62640712945591	0.0
12	7.035647279549719E-4	0.0	0.0	6.339118198874297	0.0
13	7.035647279549719E-4	0.0	0.0	6.626172607879925	0.0
14	7.035647279549719E-4	0.0	0.0	6.764305816135084	0.0
15	7.035647279549719E-4	0.0	0.0	7.0403377110694185	0.0
16	7.035647279549719E-4	0.0	0.0	7.568714821763602	0.0
17	9.380863039399625E-4	0.0	0.0	8.137664165103189	0.0
18	9.380863039399625E-4	0.0	0.0	8.762429643527204	0.0
19	9.380863039399625E-4	0.0	0.0	9.144934333958725	0.0
20	9.380863039399625E-4	0.0	0.0	9.533536585365853	0.0
21	9.380863039399625E-4	0.0	0.0	10.02110694183865	0.0
22	9.380863039399625E-4	0.0	0.0	10.51078799249531	0.0
23	9.380863039399625E-4	0.0	0.0	10.972091932457786	0.0
24	9.380863039399625E-4	0.0	0.0	11.35483114446529	0.0
25	9.380863039399625E-4	0.0	0.0	11.717870544090056	0.0
26	9.380863039399625E-4	0.0	0.0	12.041275797373359	0.0
27	9.380863039399625E-4	0.0	0.0	12.345215759849907	0.0
28	9.380863039399625E-4	0.0	0.0	12.677298311444654	0.0
29	9.380863039399625E-4	0.0	0.0	13.022045028142589	0.0
30	9.380863039399625E-4	0.0	0.0	13.38625703564728	0.0
31	9.380863039399625E-4	0.0	0.0	13.74484052532833	0.0
32	0.0011726078799249532	0.0	0.0	14.104127579737336	0.0
33	0.0014071294559099437	0.0	0.0	14.435506566604127	0.0
34	0.0014071294559099437	0.0	0.0	14.788461538461538	0.0
35	0.0014071294559099437	0.0	0.0	15.130863039399625	0.0
36	0.0014071294559099437	0.0	0.0	15.483818011257036	0.0
37	0.0014071294559099437	0.0	0.0	15.807223264540339	0.0
38	0.0014071294559099437	0.0	0.0	16.163227016885553	0.0
39	0.0014071294559099437	0.0	0.0	16.535412757973734	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	20	7.0282904E-4	45.0	6
ACCGGGT	20	7.0282904E-4	45.0	4
TTGTCGC	40	6.7975634E-9	45.0	38
ATTCGCG	20	7.0282904E-4	45.0	1
TACGCGG	20	7.0282904E-4	45.0	2
TTACGCG	20	7.0282904E-4	45.0	1
TGTCGCA	35	1.2095188E-7	45.0	39
TGCAACG	25	3.8866645E-5	45.0	1
AGTATCA	30	2.1622782E-6	44.999996	32
CGTTAGG	30	2.1622782E-6	44.999996	2
GCTTTCG	30	2.1622782E-6	44.999996	10
CGGGTCA	55	6.002665E-11	40.909092	6
ATTGCGG	50	1.0786607E-9	40.5	2
TGCTACG	40	3.451405E-7	39.375	1
GACGAGT	35	6.23905E-6	38.571426	44
AATACGG	35	6.23905E-6	38.571426	2
TAGGGAC	205	0.0	38.414635	5
CTACGAA	125	0.0	37.800003	11
TACGAAT	125	0.0	37.800003	12
GCTACGA	125	0.0	37.800003	10
>>END_MODULE