FastQCFastQC Report
Sat 18 Jun 2016
SRR3550614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150820
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170221.4791192367181663No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTT17310.1504144870613997No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCT13670.118784866443058No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA207.033292E-445.031
TATCGCG207.033292E-445.01
CGTTTTT88750.043.402821
ACGATAG650.041.5384641
CTACGAA1600.040.7812511
TACGAAT1600.040.7812512
CGAATAT1600.040.7812514
ACACGAC2000.038.2526
TGGTCGA301.1399228E-437.4999961
CACGACC2000.037.12527
GTTTTTT107650.036.9321862
CTAACGG1350.036.6666682
GCGATGT3350.036.2686589
GCTACGA1800.036.2510
AATTGCG250.002107109936.01
TATACGG250.002107109936.02
TCGCGTA250.002107109936.030
GCGTAAC250.002107109936.032
GACCGAT4950.035.4545449
CGGTCTA702.1827873E-1135.35714331
Produced by FastQC (version 0.11.5)