##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550614_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1150820
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.296806624841416	33.0	31.0	34.0	30.0	34.0
2	32.45450374515563	34.0	31.0	34.0	31.0	34.0
3	32.34399558575625	34.0	31.0	34.0	30.0	34.0
4	35.95236787681827	37.0	35.0	37.0	35.0	37.0
5	36.06674023739594	37.0	35.0	37.0	35.0	37.0
6	36.09958377504736	37.0	36.0	37.0	35.0	37.0
7	36.20711927147599	37.0	37.0	37.0	35.0	37.0
8	36.207850054743574	37.0	37.0	37.0	35.0	37.0
9	38.02705288403052	39.0	39.0	39.0	37.0	39.0
10	37.7088953963261	39.0	38.0	39.0	35.0	39.0
11	37.623500634330306	39.0	37.0	39.0	35.0	39.0
12	37.576291687666185	39.0	37.0	39.0	35.0	39.0
13	37.58172520463669	39.0	37.0	39.0	35.0	39.0
14	38.86993708833701	40.0	38.0	41.0	35.0	41.0
15	38.98274100206809	40.0	38.0	41.0	35.0	41.0
16	39.008844128534435	40.0	38.0	41.0	35.0	41.0
17	38.973364209867746	40.0	38.0	41.0	35.0	41.0
18	38.7996628490989	40.0	38.0	41.0	35.0	41.0
19	38.63030795432822	40.0	37.0	41.0	35.0	41.0
20	38.466244069446134	40.0	37.0	41.0	35.0	41.0
21	38.377824507742304	40.0	37.0	41.0	35.0	41.0
22	38.38993152708503	40.0	37.0	41.0	35.0	41.0
23	38.36135364348899	40.0	36.0	41.0	35.0	41.0
24	38.256672633426604	40.0	36.0	41.0	34.0	41.0
25	38.178489251142665	40.0	36.0	41.0	34.0	41.0
26	38.20146504231765	40.0	36.0	41.0	34.0	41.0
27	38.15554126622756	40.0	36.0	41.0	34.0	41.0
28	38.09145478875932	40.0	36.0	41.0	34.0	41.0
29	38.017683043395145	40.0	36.0	41.0	34.0	41.0
30	37.852491267096504	40.0	36.0	41.0	34.0	41.0
31	37.72485705844528	40.0	35.0	41.0	33.0	41.0
32	37.46588693279575	40.0	35.0	41.0	33.0	41.0
33	37.18804330824977	40.0	35.0	41.0	33.0	41.0
34	36.96397351453746	40.0	35.0	41.0	31.0	41.0
35	36.78607427747171	40.0	35.0	41.0	31.0	41.0
36	36.67868476390748	40.0	35.0	41.0	31.0	41.0
37	36.61713821449054	40.0	35.0	41.0	30.0	41.0
38	36.530745902921396	40.0	35.0	41.0	30.0	41.0
39	36.441978762969015	40.0	35.0	41.0	30.0	41.0
40	36.3472002572079	40.0	35.0	41.0	30.0	41.0
41	36.23374028953268	40.0	35.0	41.0	29.0	41.0
42	36.17677482143168	39.0	35.0	41.0	29.0	41.0
43	36.08237343807025	39.0	35.0	41.0	29.0	41.0
44	36.01627448254288	39.0	35.0	41.0	28.0	41.0
45	35.94545193861768	39.0	35.0	41.0	28.0	41.0
46	35.84734450218105	39.0	35.0	41.0	27.0	41.0
47	35.80013903129942	39.0	35.0	41.0	27.0	41.0
48	35.72771502059401	39.0	35.0	41.0	27.0	41.0
49	35.658271493369945	39.0	35.0	41.0	27.0	41.0
50	35.56302375697329	39.0	35.0	41.0	27.0	41.0
51	34.70083505674215	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	19.0
9	30.0
10	31.0
11	35.0
12	30.0
13	23.0
14	35.0
15	56.0
16	90.0
17	153.0
18	298.0
19	504.0
20	927.0
21	1443.0
22	2091.0
23	3340.0
24	5760.0
25	10175.0
26	15670.0
27	18482.0
28	18112.0
29	16371.0
30	16508.0
31	18062.0
32	21831.0
33	29005.0
34	56300.0
35	74809.0
36	70909.0
37	104060.0
38	198044.0
39	467015.0
40	599.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.902434785631115	20.02146295684816	23.709181279435533	17.36692097808519
2	36.7421490763108	22.73735249648077	24.28277228411046	16.237726143097962
3	30.444813263585964	22.202864044768077	32.17471020663527	15.17761248501069
4	26.792113449540327	24.572218070593145	31.368502459116108	17.26716602075042
5	24.605759371578525	28.424688482994735	29.875480092455813	17.094072052970922
6	25.476529778766444	32.44025998852992	28.29425974522514	13.788950487478493
7	77.24891816270137	5.531707825724267	13.083888010288316	4.135486001286039
8	79.38626370761718	4.769034253836395	12.503693018890877	3.3410090196555497
9	73.1448879929094	7.523591873620548	14.307971707130568	5.023548426339479
10	37.27811473557985	30.152847534801275	20.2629429450305	12.306094784588382
11	27.637597539146004	24.541283606471907	30.809596635442553	17.01152221893954
12	25.90552823204324	22.457812690081855	33.099963504283906	18.536695573591004
13	23.31120418484211	22.798526268226134	34.57838758450496	19.311881962426792
14	20.140942979788328	25.737821727116316	33.668601518916944	20.452633774178413
15	20.067343285657184	25.275455761978417	35.63858813715438	19.018612815210023
16	23.335795345927252	24.90328635234007	33.88618550251125	17.874732799221423
17	22.9803965867816	24.392085643280442	32.5105576892998	20.116960080638155
18	23.05286665160494	24.845414573955964	32.30418310422134	19.79753567021776
19	23.589440572808954	25.918127943553294	31.072452685910918	19.419978797726838
20	25.336803322848056	25.61521349993917	31.02770198640969	18.020281190803082
21	24.461253714742533	25.192210771449925	31.69774595505813	18.648789558749414
22	23.44589075615648	23.96195756069585	32.17670878156445	20.41544290158322
23	22.992388036356683	25.08524356545767	32.10597660798387	19.81639179020177
24	22.08668601518917	24.63982203993674	32.941380928381506	20.332111016492586
25	22.824681531429764	25.74885733650788	31.218609339427537	20.207851792634816
26	21.999356980240176	26.760396934359846	30.962617959368107	20.277628126031875
27	21.899080655532575	25.566987017952414	32.19834552753689	20.33558679897812
28	20.9002276637528	26.70852088076328	32.010653273318155	20.38059818216576
29	22.554439443179646	25.293529830903182	31.55515197858918	20.59687874732799
30	22.494395300742077	24.529987313393928	32.10102361794199	20.874593767922004
31	23.12160025025634	25.917085208807634	30.848177821031957	20.11313671990407
32	23.969517387601886	26.115291705045095	30.40214803357606	19.51304287377696
33	23.244903633930587	26.688361342347193	29.78276359465425	20.283971429067968
34	22.444170243826143	26.91081142142125	30.500512677916614	20.144505656835996
35	22.390034931613982	27.20521019794581	29.764255052918788	20.64049981752142
36	22.628647399245754	28.354651465911264	28.8264020437601	20.19029909108288
37	23.338141499104985	28.271145791696355	28.571540293008464	19.819172416190195
38	22.55930553865939	27.671573312942076	28.659390695330284	21.10973045306825
39	22.83615161363202	26.756399784501482	28.562329469421805	21.84511913244469
40	23.336751186110774	25.936375801602335	30.55464799012878	20.172225022158113
41	22.01569315792218	26.85789263307902	30.123477172798523	21.002937036200276
42	22.642898107436437	27.122747258476565	29.695000086894563	20.53935454719244
43	22.789836812012304	26.329486800716012	29.860099754957336	21.02057663231435
44	22.55061608244556	26.630576458525223	29.09351592777324	21.725291531255976
45	22.381084791713736	25.717922872386644	28.905128517057406	22.995863818842217
46	21.85233138110217	26.90611911506578	28.70718270450635	22.534366799325696
47	21.96642394118976	26.47381866842773	30.127474322656887	21.43228306772562
48	21.935923949879214	26.16595123477173	30.53883317981961	21.35929163552945
49	22.15524582471629	26.059070923341615	29.654941693748803	22.130741558193286
50	20.712796093220486	26.372325819850197	30.150675170747814	22.764202916181507
51	20.94541283606472	25.764150779444222	29.86018664951947	23.430249734971586
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	682.0
1	1319.0
2	1956.0
3	14960.0
4	27964.0
5	18460.0
6	8956.0
7	8668.0
8	8380.0
9	8273.0
10	8166.0
11	8050.5
12	7935.0
13	7655.0
14	7375.0
15	6894.5
16	6414.0
17	6279.5
18	6145.0
19	5888.0
20	5631.0
21	5760.5
22	5890.0
23	6332.0
24	6774.0
25	7548.0
26	10110.0
27	11898.0
28	13787.0
29	15676.0
30	18300.5
31	20925.0
32	23535.0
33	26145.0
34	29487.0
35	32829.0
36	35331.0
37	37833.0
38	40774.5
39	43716.0
40	48595.5
41	53475.0
42	57102.5
43	60730.0
44	64293.5
45	67857.0
46	75319.5
47	82782.0
48	85292.5
49	87803.0
50	87457.5
51	87112.0
52	79918.0
53	72724.0
54	68324.5
55	63925.0
56	59726.5
57	55528.0
58	53985.0
59	52442.0
60	50632.5
61	48823.0
62	44839.5
63	40856.0
64	34214.5
65	27573.0
66	22545.5
67	17518.0
68	15003.0
69	12488.0
70	9835.0
71	7182.0
72	6178.0
73	5174.0
74	4372.0
75	2704.0
76	1838.0
77	1486.0
78	1134.0
79	732.0
80	330.0
81	270.0
82	210.0
83	149.5
84	89.0
85	59.0
86	29.0
87	17.5
88	6.0
89	5.5
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1150820.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.06595422524529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.60276945724152	21.10268773670219
2	8.056833834449712	4.683591268650456
3	3.2264357142376667	2.8133829834218584
4	1.8890776469810424	2.1963137766034024
5	1.3879474376485663	2.0171008344869854
6	1.0550628300793286	1.8399844754306105
7	0.9243234213701292	1.88064395784064
8	0.8006420007119055	1.8617138994800841
9	0.7359507966887615	1.9252000951729509
>10	9.17834605061295	53.66840273827302
>50	0.11102028226582764	2.04860105330561
>100	0.02737845735221645	1.423048793217676
>500	0.002406897348745407	0.49871856854553814
>1k	0.0015043108429658794	0.5520621292702055
>5k	0.0	0.0
>10k+	3.0086216859317587E-4	1.4885476895988339
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17022	1.4791192367181663	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTT	1731	0.1504144870613997	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCT	1367	0.118784866443058	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.820510592447125E-4	0.0	0.0	0.06464955423089623	0.0
2	7.820510592447125E-4	0.0	0.0	0.25825063867503173	0.0
3	7.820510592447125E-4	0.0	0.0	0.40571071062372915	0.0
4	7.820510592447125E-4	0.0	0.0	0.6030482612398116	0.0
5	7.820510592447125E-4	0.0	0.0	1.1482247440955144	0.0
6	7.820510592447125E-4	0.0	0.0	1.7795137380302741	0.0
7	7.820510592447125E-4	0.0	0.0	2.3140021897429657	0.0
8	8.689456213830138E-4	0.0	0.0	2.994473505848004	0.0
9	8.689456213830138E-4	0.0	0.0	3.415738343094489	0.0
10	8.689456213830138E-4	0.0	0.0	4.0293877409151735	0.0
11	8.689456213830138E-4	0.0	0.0	4.692219460906136	0.0
12	8.689456213830138E-4	0.0	0.0	5.169009923358996	0.0
13	8.689456213830138E-4	0.0	0.0	5.4138787994647295	0.0
14	9.558401835213152E-4	0.0	0.0	5.558732034549278	0.0
15	9.558401835213152E-4	0.0	0.0	5.733476999009402	0.0
16	9.558401835213152E-4	0.0	0.0	5.994160685424307	0.0
17	9.558401835213152E-4	0.0	0.0	6.3189725586972765	0.0
18	9.558401835213152E-4	0.0	0.0	6.664726021445578	0.0
19	0.0010427347456596165	0.0	0.0	6.904294329260875	0.0
20	0.0010427347456596165	0.0	0.0	7.148207365183087	0.0
21	0.0010427347456596165	0.0	0.0	7.44477850576111	0.0
22	0.001129629307797918	0.0	0.0	7.790705757633687	0.0
23	0.001129629307797918	0.0	0.0	8.124380876244764	0.0
24	0.001129629307797918	0.0	0.0	8.412175666046819	0.0
25	0.001129629307797918	0.0	0.0	8.668253940668393	0.0
26	0.001129629307797918	0.0	0.0	8.902521680193253	0.0
27	0.001129629307797918	0.0	0.0	9.157383430944892	0.0
28	0.001129629307797918	0.0	0.0	9.427799308319285	0.0
29	0.001129629307797918	0.0	0.0	9.700126866060723	0.0
30	0.0012165238699362194	0.0	0.0	10.031977198866896	0.0
31	0.0012165238699362194	0.0	0.0	10.313428685632854	0.0
32	0.0012165238699362194	0.0	0.0	10.626944265827845	0.0
33	0.0012165238699362194	0.0	0.0	10.9188231000504	0.0
34	0.0012165238699362194	0.0	0.0	11.190281712170453	0.0
35	0.0012165238699362194	0.0	0.0	11.483724648511496	0.0
36	0.0012165238699362194	0.0	0.0	11.782033680332285	0.0
37	0.0012165238699362194	0.0	0.0	12.077127613353957	0.0
38	0.0013034184320745207	0.0	0.0	12.422707286977982	0.0
39	0.0013034184320745207	0.0	0.0	12.838323977685477	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	20	7.033292E-4	45.0	31
TATCGCG	20	7.033292E-4	45.0	1
CGTTTTT	8875	0.0	43.40282	1
ACGATAG	65	0.0	41.538464	1
CTACGAA	160	0.0	40.78125	11
TACGAAT	160	0.0	40.78125	12
CGAATAT	160	0.0	40.78125	14
ACACGAC	200	0.0	38.25	26
TGGTCGA	30	1.1399228E-4	37.499996	1
CACGACC	200	0.0	37.125	27
GTTTTTT	10765	0.0	36.932186	2
CTAACGG	135	0.0	36.666668	2
GCGATGT	335	0.0	36.268658	9
GCTACGA	180	0.0	36.25	10
AATTGCG	25	0.0021071099	36.0	1
TATACGG	25	0.0021071099	36.0	2
TCGCGTA	25	0.0021071099	36.0	30
GCGTAAC	25	0.0021071099	36.0	32
GACCGAT	495	0.0	35.454544	9
CGGTCTA	70	2.1827873E-11	35.357143	31
>>END_MODULE