FastQCFastQC Report
Sat 18 Jun 2016
SRR3550613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038218
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53660.5168471361505965No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCG48870.47071039030338524No Hit
GAATCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTC47560.45809261638692456No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGC32980.31765968226326263No Hit
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC25840.24888799847430887TruSeq Adapter, Index 22 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15720.15141328699752846No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC12800.12328817261885269No Hit
CCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC11760.11327100859357092TruSeq Adapter, Index 22 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCT11580.1115372686661183No Hit
CTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT11380.10961089096894872TruSeq Adapter, Index 20 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG207.032969E-445.00000421
GCGTAAG750.045.0000041
GTCGAAT351.2117744E-745.035
TATTGCG351.2117744E-745.01
TTACGCG351.2117744E-745.01
TCACGAC603.6379788E-1241.24999625
CACGACG603.6379788E-1241.24999626
TAGTGCG850.039.7058831
CGTTTTT29250.039.461541
ACGTTAG403.4577897E-739.3750041
TCGACGT403.4577897E-739.37500426
TCGACAC1200.039.37499634
TCGTGTA1950.039.2307717
CGTGTAC1950.039.2307718
GTAAGCG750.039.0000041
TGATTCG700.038.57142615
CAGTCGA356.2477066E-638.57142633
CCCCTCG1000.038.2540
CCCTCGT2000.038.2514
TATAGCG659.094947E-1238.0769271
Produced by FastQC (version 0.11.5)