##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550613_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1038218
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26098757679023	33.0	31.0	34.0	30.0	34.0
2	32.3920534993614	34.0	31.0	34.0	30.0	34.0
3	32.349080828881796	34.0	31.0	34.0	30.0	34.0
4	35.92765006963855	37.0	35.0	37.0	35.0	37.0
5	36.032188808130854	37.0	35.0	37.0	35.0	37.0
6	36.06997952260508	37.0	35.0	37.0	35.0	37.0
7	36.15178700427078	37.0	37.0	37.0	35.0	37.0
8	36.12766490274682	37.0	37.0	37.0	35.0	37.0
9	37.93977372767569	39.0	38.0	39.0	35.0	39.0
10	37.62544667882853	39.0	37.0	39.0	35.0	39.0
11	37.53983074845553	39.0	37.0	39.0	35.0	39.0
12	37.48657218426188	39.0	37.0	39.0	35.0	39.0
13	37.48081424132504	39.0	37.0	39.0	35.0	39.0
14	38.7768647817703	40.0	38.0	41.0	35.0	41.0
15	38.86101281233806	40.0	38.0	41.0	35.0	41.0
16	38.918398640747895	40.0	38.0	41.0	35.0	41.0
17	38.853266847617746	40.0	38.0	41.0	35.0	41.0
18	38.7613853737847	40.0	38.0	41.0	35.0	41.0
19	38.68660435476942	40.0	38.0	41.0	35.0	41.0
20	38.559269825797664	40.0	38.0	41.0	35.0	41.0
21	38.52217453367212	40.0	38.0	41.0	35.0	41.0
22	38.5234806177508	40.0	38.0	41.0	35.0	41.0
23	38.47740647917875	40.0	38.0	41.0	35.0	41.0
24	38.40474254925266	40.0	37.0	41.0	34.0	41.0
25	38.358733907522314	40.0	37.0	41.0	34.0	41.0
26	38.366091707136654	40.0	37.0	41.0	34.0	41.0
27	38.30124116515029	40.0	37.0	41.0	34.0	41.0
28	38.25322523785949	40.0	37.0	41.0	34.0	41.0
29	38.19535974140306	40.0	37.0	41.0	34.0	41.0
30	38.09110995956533	40.0	37.0	41.0	34.0	41.0
31	38.05470334746652	40.0	37.0	41.0	34.0	41.0
32	37.88509253355269	40.0	37.0	41.0	34.0	41.0
33	37.773527332409955	40.0	37.0	41.0	33.0	41.0
34	37.67243873637329	40.0	36.0	41.0	33.0	41.0
35	37.592332246214184	40.0	36.0	41.0	33.0	41.0
36	37.55213067005195	40.0	36.0	41.0	33.0	41.0
37	37.48597404398691	40.0	36.0	41.0	33.0	41.0
38	37.43277616069072	40.0	36.0	41.0	33.0	41.0
39	37.34954893866221	40.0	36.0	41.0	33.0	41.0
40	37.26772411959723	40.0	35.0	41.0	33.0	41.0
41	37.1927437204903	40.0	35.0	41.0	32.0	41.0
42	37.17592933276056	40.0	35.0	41.0	33.0	41.0
43	37.1020527480741	40.0	35.0	41.0	32.0	41.0
44	37.05920529214481	40.0	35.0	41.0	32.0	41.0
45	36.98318079632601	40.0	35.0	41.0	32.0	41.0
46	36.8698693338008	39.0	35.0	41.0	32.0	41.0
47	36.78137443195938	39.0	35.0	41.0	31.0	41.0
48	36.76169552059394	39.0	35.0	41.0	32.0	41.0
49	36.70647879347112	39.0	35.0	41.0	31.0	41.0
50	36.61705152482427	39.0	35.0	41.0	31.0	41.0
51	35.73527621366611	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	13.0
9	23.0
10	25.0
11	23.0
12	20.0
13	21.0
14	23.0
15	26.0
16	66.0
17	92.0
18	197.0
19	369.0
20	728.0
21	1288.0
22	2092.0
23	3010.0
24	4163.0
25	5789.0
26	7674.0
27	8999.0
28	9536.0
29	10165.0
30	11750.0
31	14636.0
32	19021.0
33	25754.0
34	51241.0
35	72951.0
36	63739.0
37	95299.0
38	180883.0
39	448007.0
40	593.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.22542857087818	21.03113218996396	24.173343170702108	12.570096068455758
2	32.03672061166344	26.32712975502255	24.897564865953008	16.738584767360997
3	31.177748796495536	25.860657395652936	27.349939993334733	15.611653814516796
4	28.217773145909625	24.876278392399286	28.667967613738156	18.237980847952933
5	27.16317767559414	29.433221153938767	24.970381942906016	18.43321922756107
6	26.151347790155828	35.378311684058644	24.183071378072814	14.287269147712717
7	78.99015428358976	6.14890129048042	10.523319765213087	4.337624660716728
8	80.1260428927258	7.137422005782986	8.604262303292758	4.132272798198452
9	73.77795414835805	7.961141109092695	10.512050455684644	7.748854286864608
10	38.92872209882703	30.531352760210282	17.430154360644874	13.109770780317815
11	28.9320739960201	24.509014484433905	27.57041392077579	18.988497598770202
12	26.436740645991495	22.746378891523744	30.23777279916164	20.579107663323118
13	24.765607993696893	23.967606032644394	31.403809219258385	19.86297675440033
14	20.498296118926852	28.00991699238503	29.893336466907723	21.598450421780395
15	20.38078707939951	25.824826770485583	33.01628367067417	20.778102479440737
16	22.920716073117593	26.267412046410293	29.93340512300885	20.87846675746327
17	23.308303265788112	26.469489066843384	28.47321082855431	21.7489968388142
18	23.029074818583382	25.688342910641115	29.725934245023684	21.55664802575182
19	23.903650293098366	26.583626945400677	27.692642585661197	21.820080175839756
20	25.498113113045623	26.38203151939188	29.393537773377076	18.72631759418542
21	24.40055942008326	27.680891681708463	28.466950101038513	19.451598797169765
22	23.67932361026297	24.460758723119806	28.94305434889397	22.91686331772325
23	22.89673267078783	26.592680920577372	29.281422591401807	21.22916381723299
24	22.721047024805966	25.473744435176428	30.073838057132505	21.731370482885097
25	22.23020598756716	28.696092728116827	27.498078438247077	21.57562284606894
26	21.19371846760507	27.784723439585907	28.62905478425533	22.392503308553696
27	22.196494377866692	27.0899753231017	29.011826032682926	21.70170426634869
28	20.083739638495963	27.647180072008	30.517771797445235	21.751308492050804
29	21.829808383210462	26.042025855841448	29.252912201483696	22.87525355946439
30	21.85976355640145	26.73687029121052	29.922810045674414	21.480556106713617
31	22.144289542273395	26.88231180734682	28.455295515970636	22.518103134409152
32	23.032445979553426	26.952624593293507	27.537761818808765	22.4771676083443
33	22.852040708213497	26.14479810598545	27.675690461926106	23.327470723874946
34	20.68977806202551	27.19823774968263	29.595422156040446	22.516562032251418
35	21.458595400965883	25.96660816899726	29.1587123320921	23.416084097944747
36	21.495389214981824	28.49603840426577	27.709209433856856	22.299362946895545
37	20.77473131847069	26.878748008607054	29.430331587393017	22.916189085529243
38	21.090849898576213	27.27442598760569	27.52899680028664	24.105727313531457
39	22.56934478115386	25.378870333590825	28.668160251507874	23.38362463374744
40	22.442685447564962	25.436276388966476	29.529540038797247	22.59149812467131
41	19.732849940956523	26.283112024642225	28.808496866746676	25.175541167654576
42	20.56494878724892	26.28330466241194	29.10939706304456	24.042349487294576
43	22.388554234274498	24.8690544760349	28.51828806666808	24.224103223022524
44	21.529004505797435	25.508611871495194	28.51809542889836	24.444288193809008
45	21.663273031290153	24.945050076188238	28.38681278883626	25.004864103685353
46	22.153247198565236	26.909859008416344	27.95154774816079	22.985346044857632
47	20.328293287151638	25.66715275597225	30.68921941249333	23.31533454438278
48	20.634876297656177	25.00948741015856	29.690970489820057	24.664665802365207
49	21.0486622270082	24.35991285067298	30.964402466534004	23.627022455784815
50	20.419796227767193	24.482526791097822	30.072778549399064	25.024898431735913
51	20.36229385350668	24.07750588026792	28.93351877929298	26.62668148693242
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	470.0
1	1361.0
2	2252.0
3	5949.0
4	9646.0
5	6465.5
6	3285.0
7	3111.5
8	2938.0
9	2919.5
10	2901.0
11	2944.0
12	2987.0
13	2923.0
14	2859.0
15	2688.0
16	2517.0
17	2723.5
18	2930.0
19	2925.5
20	2921.0
21	3269.5
22	3618.0
23	4049.5
24	4481.0
25	6067.0
26	9073.5
27	10494.0
28	12425.0
29	14356.0
30	16788.0
31	19220.0
32	21656.0
33	24092.0
34	27849.5
35	31607.0
36	33493.0
37	35379.0
38	38412.5
39	41446.0
40	46389.5
41	51333.0
42	54016.0
43	56699.0
44	62742.0
45	68785.0
46	82262.5
47	95740.0
48	94163.0
49	92586.0
50	88902.5
51	85219.0
52	76325.0
53	67431.0
54	61693.5
55	55956.0
56	53514.5
57	51073.0
58	47863.5
59	44654.0
60	41665.0
61	38676.0
62	35144.0
63	31612.0
64	27375.5
65	23139.0
66	20276.0
67	17413.0
68	14759.5
69	12106.0
70	9742.5
71	7379.0
72	5935.5
73	4492.0
74	3506.0
75	2193.5
76	1867.0
77	1228.0
78	589.0
79	520.5
80	452.0
81	319.5
82	187.0
83	170.0
84	153.0
85	87.5
86	22.0
87	34.5
88	47.0
89	34.5
90	22.0
91	14.0
92	6.0
93	3.0
94	0.0
95	0.5
96	1.0
97	3.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1038218.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.65171250086431
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.24084178927365	18.531013043988718
2	9.391001064400943	4.817905187986474
3	3.209779172024932	2.470089975361376
4	1.675809492017023	1.7194953318176058
5	1.0489496573957786	1.3453677519698315
6	0.8203432209380138	1.2625925073320965
7	0.6677893811820225	1.199095885204793
8	0.5881688461862259	1.207003051546732
9	0.5353028760425815	1.2358291929417096
>10	9.531185376163712	56.36629883225209
>50	0.22438644656790274	3.4957088568375623
>100	0.05733053913652619	2.6932917507956478
>500	0.005315414224578507	1.0057721816180811
>1k	0.0034170520015147548	2.1279283815409564
>5k	3.796724446127505E-4	0.5226080688062964
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5366	0.5168471361505965	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCG	4887	0.47071039030338524	No Hit
GAATCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTC	4756	0.45809261638692456	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGC	3298	0.31765968226326263	No Hit
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	2584	0.24888799847430887	TruSeq Adapter, Index 22 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1572	0.15141328699752846	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1280	0.12328817261885269	No Hit
CCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1176	0.11327100859357092	TruSeq Adapter, Index 22 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCT	1158	0.1115372686661183	No Hit
CTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT	1138	0.10961089096894872	TruSeq Adapter, Index 20 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9263776971695733E-4	0.0	0.0	0.11452315409673113	0.0
2	1.9263776971695733E-4	0.0	0.0	0.6030525380989349	0.0
3	1.9263776971695733E-4	0.0	0.0	0.8116792427023997	0.0
4	1.9263776971695733E-4	0.0	0.0	1.1849149215290045	0.0
5	1.9263776971695733E-4	0.0	0.0	2.3912126354965912	0.0
6	2.88956654575436E-4	0.0	0.0	2.957760316234163	0.0
7	2.88956654575436E-4	0.0	0.0	3.554263170162721	0.0
8	2.88956654575436E-4	0.0	0.0	4.3114259240352215	0.0
9	2.88956654575436E-4	0.0	0.0	4.618779485618627	0.0
10	2.88956654575436E-4	0.0	0.0	5.702077983621937	0.0
11	2.88956654575436E-4	0.0	0.0	6.606415993558193	0.0
12	2.88956654575436E-4	0.0	0.0	7.619112748960237	0.0
13	3.8527553943391466E-4	0.0	0.0	7.925984716119351	0.0
14	3.8527553943391466E-4	0.0	0.0	8.057267356181457	0.0
15	3.8527553943391466E-4	0.0	0.0	8.353736883775854	0.0
16	3.8527553943391466E-4	0.0	0.0	8.813563240090232	0.0
17	3.8527553943391466E-4	0.0	0.0	9.369419524608512	0.0
18	4.8159442429239333E-4	0.0	0.0	9.932788682145754	0.0
19	5.77913309150872E-4	0.0	0.0	10.378167205731359	0.0
20	5.77913309150872E-4	0.0	0.0	10.765754398401876	0.0
21	5.77913309150872E-4	0.0	0.0	11.22586971137083	0.0
22	5.77913309150872E-4	0.0	0.0	11.76901190308779	0.0
23	5.77913309150872E-4	0.0	0.0	12.251376878459052	0.0
24	5.77913309150872E-4	0.0	0.0	12.650426018427728	0.0
25	5.77913309150872E-4	0.0	0.0	13.01508931650193	0.0
26	5.77913309150872E-4	0.0	0.0	13.364245274113914	0.0
27	5.77913309150872E-4	0.0	0.0	13.710993259604438	0.0
28	6.742321940093507E-4	0.0	0.0	14.07748661649095	0.0
29	6.742321940093507E-4	0.0	0.0	14.452167078590431	0.0
30	6.742321940093507E-4	0.0	0.0	14.882038261713822	0.0
31	7.705510788678293E-4	0.0	0.0	15.263750002407972	0.0
32	8.66869963726308E-4	0.0	0.0	15.63428875245854	0.0
33	8.66869963726308E-4	0.0	0.0	16.00839130124887	0.0
34	8.66869963726308E-4	0.0	0.0	16.36428958080095	0.0
35	8.66869963726308E-4	0.0	0.0	16.740511145058168	0.0
36	8.66869963726308E-4	0.0	0.0	17.10999038737529	0.0
37	8.66869963726308E-4	0.0	0.0	17.481588645159302	0.0
38	8.66869963726308E-4	0.0	0.0	17.847215132082088	0.0
39	8.66869963726308E-4	0.0	0.0	18.213419532314024	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	20	7.032969E-4	45.000004	21
GCGTAAG	75	0.0	45.000004	1
GTCGAAT	35	1.2117744E-7	45.0	35
TATTGCG	35	1.2117744E-7	45.0	1
TTACGCG	35	1.2117744E-7	45.0	1
TCACGAC	60	3.6379788E-12	41.249996	25
CACGACG	60	3.6379788E-12	41.249996	26
TAGTGCG	85	0.0	39.705883	1
CGTTTTT	2925	0.0	39.46154	1
ACGTTAG	40	3.4577897E-7	39.375004	1
TCGACGT	40	3.4577897E-7	39.375004	26
TCGACAC	120	0.0	39.374996	34
TCGTGTA	195	0.0	39.23077	17
CGTGTAC	195	0.0	39.23077	18
GTAAGCG	75	0.0	39.000004	1
TGATTCG	70	0.0	38.571426	15
CAGTCGA	35	6.2477066E-6	38.571426	33
CCCCTCG	100	0.0	38.25	40
CCCTCGT	200	0.0	38.25	14
TATAGCG	65	9.094947E-12	38.076927	1
>>END_MODULE