FastQCFastQC Report
Sat 18 Jun 2016
SRR3550612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517654
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCG24860.48024356037044047No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23590.45570979843679366No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGC19780.3821085126358533No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTC19710.38075625804108537No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT11570.22350836659235707No Hit
GCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC11330.2188720651245813No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA9090.17559991809200742No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA8520.16458870210603996No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7590.1466230339184088No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7490.14469124164016892No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7400.142952628589753No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCC6590.12730511113600976No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6450.1246006019464739No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCT5440.10508949993625084No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT5410.10450996225277889No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCC207.029691E-445.010
ACACGGC207.029691E-445.014
ACGTTAG207.029691E-445.01
ACGGCAA207.029691E-445.031
GCTTACG253.8878243E-545.01
TTAACGG207.029691E-445.02
CGGTATT207.029691E-445.043
CCTATTG207.029691E-445.04
CAATCGA253.8878243E-545.041
AACACGT207.029691E-445.041
ACGAACG253.8878243E-545.01
GCGATTC302.1631822E-644.9999969
TCGACGG302.1631822E-644.9999962
CTACGAA1550.043.54838611
CGAATAT1550.043.54838614
GCTACGA1550.042.0967710
CGAATGC650.041.53846445
GCGATTA603.6379788E-1241.2499969
TTGAGCG501.0786607E-940.51
AATATAC1700.039.70588316