##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550612_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	517654
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.237734084929315	33.0	31.0	34.0	30.0	34.0
2	32.36459681563361	34.0	31.0	34.0	30.0	34.0
3	32.3297260332191	34.0	31.0	34.0	30.0	34.0
4	35.93333578027022	37.0	35.0	37.0	35.0	37.0
5	36.020440294096055	37.0	35.0	37.0	35.0	37.0
6	36.05755775093016	37.0	35.0	37.0	35.0	37.0
7	36.10386281183957	37.0	36.0	37.0	35.0	37.0
8	36.069210321952504	37.0	36.0	37.0	35.0	37.0
9	37.87287068196131	39.0	38.0	39.0	35.0	39.0
10	37.6133768888099	39.0	37.0	39.0	35.0	39.0
11	37.518046803463314	39.0	37.0	39.0	35.0	39.0
12	37.383312019225194	39.0	37.0	39.0	35.0	39.0
13	37.305016478188136	39.0	37.0	39.0	34.0	39.0
14	38.574916836342425	40.0	38.0	41.0	35.0	41.0
15	38.67717046521422	40.0	38.0	41.0	35.0	41.0
16	38.75493476337476	40.0	38.0	41.0	35.0	41.0
17	38.679720431021494	40.0	38.0	41.0	35.0	41.0
18	38.594503278251494	40.0	38.0	41.0	35.0	41.0
19	38.50798023390141	40.0	37.0	41.0	35.0	41.0
20	38.351037565632645	40.0	37.0	41.0	34.0	41.0
21	38.300457448411485	40.0	37.0	41.0	34.0	41.0
22	38.31166957079439	40.0	37.0	41.0	34.0	41.0
23	38.24790497127425	40.0	37.0	41.0	34.0	41.0
24	38.15891502818485	40.0	36.0	41.0	34.0	41.0
25	38.09959161911238	40.0	36.0	41.0	34.0	41.0
26	38.10258976072821	40.0	36.0	41.0	34.0	41.0
27	37.995323130894384	40.0	36.0	41.0	34.0	41.0
28	37.93625665019492	40.0	36.0	41.0	34.0	41.0
29	37.889480231969614	40.0	36.0	41.0	34.0	41.0
30	37.79046428695615	40.0	36.0	41.0	33.0	41.0
31	37.71496018576114	40.0	36.0	41.0	33.0	41.0
32	37.52850359506543	40.0	35.0	41.0	33.0	41.0
33	37.36928141190834	40.0	35.0	41.0	33.0	41.0
34	37.26805549652857	40.0	35.0	41.0	33.0	41.0
35	37.184350937112434	40.0	35.0	41.0	33.0	41.0
36	37.104440417730764	40.0	35.0	41.0	32.0	41.0
37	37.03067106600162	40.0	35.0	41.0	32.0	41.0
38	36.95723977792116	40.0	35.0	41.0	32.0	41.0
39	36.864987037673814	40.0	35.0	41.0	32.0	41.0
40	36.793568677147285	40.0	35.0	41.0	31.0	41.0
41	36.683300042113075	39.0	35.0	41.0	31.0	41.0
42	36.646549625811836	39.0	35.0	41.0	31.0	41.0
43	36.54229466013978	39.0	35.0	41.0	31.0	41.0
44	36.48678074544	39.0	35.0	41.0	31.0	41.0
45	36.410743469576204	39.0	35.0	41.0	31.0	41.0
46	36.28720921696732	39.0	35.0	41.0	31.0	41.0
47	36.20979843679369	39.0	35.0	41.0	31.0	41.0
48	36.16136260900138	39.0	35.0	41.0	31.0	41.0
49	36.098378453561644	38.0	35.0	41.0	30.0	41.0
50	35.98244966715219	38.0	35.0	41.0	30.0	41.0
51	35.094366120999744	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	7.0
10	19.0
11	18.0
12	15.0
13	15.0
14	20.0
15	21.0
16	36.0
17	58.0
18	143.0
19	256.0
20	471.0
21	932.0
22	1433.0
23	2036.0
24	2669.0
25	3441.0
26	4284.0
27	5380.0
28	5352.0
29	5754.0
30	6625.0
31	8146.0
32	10259.0
33	14241.0
34	28921.0
35	43255.0
36	33709.0
37	49847.0
38	86956.0
39	203022.0
40	303.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.233793228681705	20.541326832208387	23.321562279051257	11.903317660058649
2	32.2068408628157	27.067693865014082	24.017973395356744	16.70749187681347
3	31.58789461686764	26.336510487700277	26.434645535434864	15.640949359997219
4	29.353776847083186	24.40259323795431	27.89778500697377	18.345844907988734
5	26.77966363632851	30.277559914537512	24.14894891182141	18.79382753731257
6	26.181001209301968	36.95151587740074	23.318471411406076	13.549011501891226
7	76.91682088808355	7.367276211523527	11.150305030000734	4.5655978703921924
8	77.08083005250612	9.272409756323723	9.422510016342962	4.224250174827201
9	70.90392424283401	9.597916755207146	11.225065391168618	8.27309361079022
10	42.97696917245882	25.210275589486415	17.920464248320307	13.892290989734457
11	33.150328211508075	24.836280604419166	23.0601907838054	18.95320040026736
12	29.416366916898163	22.427335633454007	27.649356519992118	20.506940929655716
13	25.62831543849753	25.583884216098014	29.125245820567407	19.662554524837052
14	20.70900640195961	29.47219571373929	28.64326364714655	21.175534237154547
15	20.718858542578634	25.287740459843832	33.182395963326854	20.811005034250677
16	23.04860003013596	25.128947134572517	30.250708001869974	21.57174483342155
17	22.539572764819745	26.34443083604106	28.302688668492852	22.81330773064634
18	23.63006950588617	25.927356883169068	28.660842956878536	21.78173065406623
19	26.030321411599257	26.514428556526177	25.982606142326723	21.472643889547847
20	27.77086625429341	25.645315210546038	27.52997175719689	19.05384677796366
21	25.021539483902377	27.325781313386933	27.93371634334903	19.718962859361657
22	23.781908378955826	25.536555305281134	27.491529090859917	23.19000722490312
23	24.45571752560591	26.696017030680725	27.53480123789249	21.313464205820875
24	24.066075023084917	25.57750930157982	28.263086926788937	22.093328748546327
25	22.65663937688108	28.115884355187053	27.104977455984113	22.12249881194775
26	22.673639148929595	27.548710142295818	27.73763942710768	22.040011281666906
27	23.975860323691116	27.43125717177883	27.52127869194481	21.07160381258524
28	21.195818056076064	27.580005177203304	29.333106669706023	21.89107009701461
29	23.706182121648823	26.1023772635776	28.495867896316845	21.69557271845673
30	22.294621503938924	27.612072929022087	27.181090071746766	22.912215495292223
31	24.858882574074574	27.448836481510817	24.560807025542157	23.13147391887245
32	25.736688985306788	28.05271474768861	25.168935234732082	21.041661032272522
33	23.802578556332996	27.374655658026402	25.0934021566529	23.729363628987702
34	22.445108122413814	27.81259296750339	27.29506581616292	22.447233093919877
35	24.3662755431234	26.28338620004868	25.739973032179797	23.610365224648124
36	23.66039864465454	28.384403481862403	25.935084052282026	22.020113821201033
37	23.59046776418225	28.50224281083504	26.676892287126147	21.23039713785656
38	21.8982177284441	29.25873266699378	25.08026596916087	23.762783635401252
39	24.2277660367736	25.226116286167983	27.70209444918807	22.844023227870352
40	22.45843748913367	25.95652694657049	28.197019630873132	23.38801593342271
41	20.632700606969134	26.823901679500207	28.062566888307632	24.480830825223027
42	21.774196664181094	27.79752498773312	27.64027709628439	22.788001251801397
43	24.394286531157878	24.768861053908594	27.34336062311892	23.49349179181461
44	22.062999609777957	26.781981787062403	27.13240890633512	24.02260969682452
45	23.172621094398963	26.434259176979218	26.281454407770443	24.11166532085138
46	23.394970385624376	28.02470375965413	25.97545851089724	22.604867343824253
47	22.471766855853524	26.407986801995154	29.038508347274433	22.08173799487689
48	22.04174989471732	26.069536794847526	28.30172277235373	23.58699053808142
49	22.006204916797707	25.91847063868916	29.085257720407842	22.99006672410529
50	20.987570848481806	26.00404903661519	28.653308966993396	24.35507114790961
51	21.704652142164456	25.35747816108829	26.91353684121054	26.02433285553671
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	442.0
1	973.0
2	1504.0
3	3051.0
4	4598.0
5	3211.0
6	1824.0
7	1705.0
8	1586.0
9	1605.0
10	1624.0
11	1698.0
12	1772.0
13	1752.0
14	1732.0
15	1703.0
16	1674.0
17	1701.0
18	1728.0
19	1702.0
20	1676.0
21	1938.5
22	2201.0
23	2234.0
24	2267.0
25	3046.0
26	4535.5
27	5246.0
28	6795.5
29	8345.0
30	8935.0
31	9525.0
32	10196.5
33	10868.0
34	12330.0
35	13792.0
36	13767.5
37	13743.0
38	14694.0
39	15645.0
40	18693.5
41	21742.0
42	23945.0
43	26148.0
44	28890.5
45	31633.0
46	38720.0
47	45807.0
48	43892.5
49	41978.0
50	40551.0
51	39124.0
52	36144.5
53	33165.0
54	31001.0
55	28837.0
56	27643.5
57	26450.0
58	26004.0
59	25558.0
60	24319.0
61	23080.0
62	21610.5
63	20141.0
64	17840.0
65	15539.0
66	13112.0
67	10685.0
68	9361.0
69	8037.0
70	6698.0
71	5359.0
72	4606.0
73	3853.0
74	2984.5
75	1743.5
76	1371.0
77	1126.0
78	881.0
79	584.0
80	287.0
81	201.5
82	116.0
83	99.0
84	82.0
85	53.5
86	25.0
87	17.5
88	10.0
89	10.0
90	10.0
91	6.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	517654.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.709960473399285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.05125530482302	20.51957357502245
2	8.593543378976436	4.762534947157583
3	2.949854523516121	2.452210567467293
4	1.4967798391428022	1.6590284072011197
5	1.0022478119471259	1.3886123626802886
6	0.8096372392831526	1.3461009538996966
7	0.6581570935043131	1.276625493240431
8	0.5925770995170516	1.3136230404047275
9	0.5455377639036467	1.360514688706501
>10	9.067551496895963	54.6650379389964
>50	0.16502881034154265	2.861576469185059
>100	0.05664124422739388	2.906918349858766
>500	0.006992746200912825	1.339910704524288
>1k	0.004195647720547695	2.147732501655417
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCG	2486	0.48024356037044047	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2359	0.45570979843679366	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGC	1978	0.3821085126358533	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTC	1971	0.38075625804108537	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1157	0.22350836659235707	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC	1133	0.2188720651245813	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	909	0.17559991809200742	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	852	0.16458870210603996	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	759	0.1466230339184088	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	749	0.14469124164016892	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	740	0.142952628589753	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCC	659	0.12730511113600976	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	645	0.1246006019464739	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCT	544	0.10508949993625084	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	541	0.10450996225277889	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.727169112959622E-4	0.0	0.0	0.09658961391199528	0.0
2	7.727169112959622E-4	0.0	0.0	0.5202316605300066	0.0
3	7.727169112959622E-4	0.0	0.0	0.6892634848759982	0.0
4	7.727169112959622E-4	0.0	0.0	0.9707256198155525	0.0
5	7.727169112959622E-4	0.0	0.0	2.0108025824199176	0.0
6	7.727169112959622E-4	0.0	0.0	2.407013178686922	0.0
7	7.727169112959622E-4	0.0	0.0	2.9081200956623534	0.0
8	7.727169112959622E-4	0.0	0.0	3.5685998755925774	0.0
9	7.727169112959622E-4	0.0	0.0	3.811812523422981	0.0
10	7.727169112959622E-4	0.0	0.0	4.849764514521283	0.0
11	7.727169112959622E-4	0.0	0.0	5.769683997419126	0.0
12	7.727169112959622E-4	0.0	0.0	6.842601428753569	0.0
13	7.727169112959622E-4	0.0	0.0	7.15149501404413	0.0
14	7.727169112959622E-4	0.0	0.0	7.288652265799163	0.0
15	7.727169112959622E-4	0.0	0.0	7.600443539507084	0.0
16	7.727169112959622E-4	0.0	0.0	8.066778195474196	0.0
17	9.658961391199527E-4	0.0	0.0	8.5636351694375	0.0
18	9.658961391199527E-4	0.0	0.0	9.112070997229809	0.0
19	9.658961391199527E-4	0.0	0.0	9.546337901378141	0.0
20	9.658961391199527E-4	0.0	0.0	9.902946755941228	0.0
21	9.658961391199527E-4	0.0	0.0	10.335475047039141	0.0
22	9.658961391199527E-4	0.0	0.0	10.794082533893295	0.0
23	9.658961391199527E-4	0.0	0.0	11.225644928852091	0.0
24	9.658961391199527E-4	0.0	0.0	11.596355867046329	0.0
25	0.0011590753669439432	0.0	0.0	11.931328648093128	0.0
26	0.0011590753669439432	0.0	0.0	12.232108705815081	0.0
27	0.0011590753669439432	0.0	0.0	12.53172968817009	0.0
28	0.0011590753669439432	0.0	0.0	12.840816452688475	0.0
29	0.0011590753669439432	0.0	0.0	13.165550734660604	0.0
30	0.0011590753669439432	0.0	0.0	13.536454852082665	0.0
31	0.0011590753669439432	0.0	0.0	13.881666132204137	0.0
32	0.0011590753669439432	0.0	0.0	14.227843308464728	0.0
33	0.0011590753669439432	0.0	0.0	14.577884069281799	0.0
34	0.0011590753669439432	0.0	0.0	14.926379396276277	0.0
35	0.0011590753669439432	0.0	0.0	15.300953919026995	0.0
36	0.0013522545947679337	0.0	0.0	15.663551329652625	0.0
37	0.0013522545947679337	0.0	0.0	16.01378526969752	0.0
38	0.0015454338225919244	0.0	0.0	16.355326144490334	0.0
39	0.0015454338225919244	0.0	0.0	16.70420782994046	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCC	20	7.029691E-4	45.0	10
ACACGGC	20	7.029691E-4	45.0	14
ACGTTAG	20	7.029691E-4	45.0	1
ACGGCAA	20	7.029691E-4	45.0	31
GCTTACG	25	3.8878243E-5	45.0	1
TTAACGG	20	7.029691E-4	45.0	2
CGGTATT	20	7.029691E-4	45.0	43
CCTATTG	20	7.029691E-4	45.0	4
CAATCGA	25	3.8878243E-5	45.0	41
AACACGT	20	7.029691E-4	45.0	41
ACGAACG	25	3.8878243E-5	45.0	1
GCGATTC	30	2.1631822E-6	44.999996	9
TCGACGG	30	2.1631822E-6	44.999996	2
CTACGAA	155	0.0	43.548386	11
CGAATAT	155	0.0	43.548386	14
GCTACGA	155	0.0	42.09677	10
CGAATGC	65	0.0	41.538464	45
GCGATTA	60	3.6379788E-12	41.249996	9
TTGAGCG	50	1.0786607E-9	40.5	1
AATATAC	170	0.0	39.705883	16
>>END_MODULE