FastQCFastQC Report
Sat 18 Jun 2016
SRR3550609_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550609_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206628
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63130.5231935608986366No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC37040.3069711626118406TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC30770.25500817153256844TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC28990.24025631760575752TruSeq Adapter, Index 16 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG27900.23122287896518232No Hit
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT20810.17246409000951413TruSeq Adapter, Index 27 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC19060.15796086283427865No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT16350.13550157960862835No Hit
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG14050.11644019532117604TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCC13710.11362242546998744No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGTA207.0334255E-445.00000420
CGTCCTA253.890922E-545.036
CGGTCTA600.044.99999631
AGTCGAG302.1655924E-644.9999961
CGTTTTT35700.040.399161
CTACGAA2750.039.27272811
CGCATCG356.248547E-638.5714321
ACGGGCC1400.038.571435
AGTTCGG1050.038.571432
CATCGTT356.248547E-638.5714336
CGAATAT2850.037.89473714
GCGATCG301.1399556E-437.4999969
TGTCGCG301.1399556E-437.4999962
TACGAAT2900.037.2413812
GCCGATT850.037.0588269
CGTAAGG1800.036.2500042
AAATCGT250.002107150236.010
CTCAACG504.879257E-836.01
TAGCGAT250.002107150236.033
ATTAACG250.002107150236.01
Produced by FastQC (version 0.11.5)