##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550609_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1206628
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20254378317095	33.0	31.0	34.0	30.0	34.0
2	32.33184958413032	34.0	31.0	34.0	30.0	34.0
3	32.29337045054483	34.0	31.0	34.0	30.0	34.0
4	35.88830028807553	37.0	35.0	37.0	35.0	37.0
5	36.00743311111627	37.0	35.0	37.0	35.0	37.0
6	36.05572305631893	37.0	35.0	37.0	35.0	37.0
7	36.178035815512324	37.0	37.0	37.0	35.0	37.0
8	36.161266769874395	37.0	37.0	37.0	35.0	37.0
9	37.98701008098602	39.0	38.0	39.0	35.0	39.0
10	37.63565489943877	39.0	37.0	39.0	35.0	39.0
11	37.547339362255805	39.0	37.0	39.0	35.0	39.0
12	37.529310607743234	39.0	37.0	39.0	35.0	39.0
13	37.5387733419082	39.0	37.0	39.0	35.0	39.0
14	38.818083949651424	40.0	38.0	41.0	35.0	41.0
15	38.90610942229088	40.0	38.0	41.0	35.0	41.0
16	38.96426073321687	40.0	38.0	41.0	35.0	41.0
17	38.927639670221474	40.0	38.0	41.0	35.0	41.0
18	38.839132690439804	40.0	38.0	41.0	35.0	41.0
19	38.76311423238977	40.0	38.0	41.0	35.0	41.0
20	38.66974328459144	40.0	38.0	41.0	35.0	41.0
21	38.60448953612878	40.0	38.0	41.0	35.0	41.0
22	38.605605041487514	40.0	38.0	41.0	35.0	41.0
23	38.52978134105955	40.0	38.0	41.0	35.0	41.0
24	38.42817090271401	40.0	37.0	41.0	34.0	41.0
25	38.305218344013234	40.0	37.0	41.0	34.0	41.0
26	38.27227281316197	40.0	37.0	41.0	34.0	41.0
27	38.20728758159101	40.0	37.0	41.0	34.0	41.0
28	38.09739952992969	40.0	37.0	41.0	34.0	41.0
29	38.04097866119467	40.0	37.0	41.0	34.0	41.0
30	37.910281379182315	40.0	36.0	41.0	34.0	41.0
31	37.78346101698286	40.0	36.0	41.0	33.0	41.0
32	37.65167392104277	40.0	36.0	41.0	33.0	41.0
33	37.49488077518506	40.0	36.0	41.0	33.0	41.0
34	37.48764490795838	40.0	36.0	41.0	33.0	41.0
35	37.44176001219929	40.0	36.0	41.0	33.0	41.0
36	37.41733989265954	40.0	36.0	41.0	33.0	41.0
37	37.40326927603205	40.0	36.0	41.0	33.0	41.0
38	37.36255498794989	40.0	36.0	41.0	33.0	41.0
39	37.31190723238645	40.0	36.0	41.0	33.0	41.0
40	37.22342345776826	40.0	36.0	41.0	32.0	41.0
41	37.12823504841592	40.0	35.0	41.0	32.0	41.0
42	37.10364669144094	40.0	35.0	41.0	32.0	41.0
43	37.01706325396062	40.0	35.0	41.0	31.0	41.0
44	36.94173432076829	40.0	35.0	41.0	31.0	41.0
45	36.87145665441213	39.0	35.0	41.0	31.0	41.0
46	36.78319250009116	39.0	35.0	41.0	31.0	41.0
47	36.73950712232768	39.0	35.0	41.0	31.0	41.0
48	36.71711994085998	39.0	35.0	41.0	31.0	41.0
49	36.69110280881929	39.0	35.0	41.0	31.0	41.0
50	36.60642799603523	39.0	35.0	41.0	31.0	41.0
51	35.62793006626732	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	30.0
10	26.0
11	23.0
12	19.0
13	22.0
14	23.0
15	33.0
16	65.0
17	105.0
18	168.0
19	325.0
20	592.0
21	1088.0
22	1728.0
23	2837.0
24	4318.0
25	6615.0
26	9192.0
27	10899.0
28	11428.0
29	12764.0
30	14876.0
31	18339.0
32	23696.0
33	32546.0
34	57989.0
35	81808.0
36	75792.0
37	118719.0
38	241568.0
39	478202.0
40	784.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.293588413330376	20.695110672054685	24.823475006381422	13.187825908233524
2	31.967847588486258	25.267356633527484	25.580377713760992	17.184418064225262
3	30.387078701969454	24.267462714274824	29.00529409229688	16.340164491458843
4	27.148715262699024	25.473716837335118	28.982834809071228	18.394733090894626
5	24.981767371551133	29.426633560633437	26.77411762365866	18.817481444156776
6	25.744139867465364	33.79193918921159	25.59496381652009	14.86895712680296
7	79.76750083704339	5.544625186884441	9.951782985311132	4.73609099076103
8	81.14597042336163	5.441693711732199	8.974762727203412	4.4375731377027545
9	75.12721402122278	7.611293621563564	10.7989372035126	6.462555153701058
10	36.33953463702152	33.38634608180814	17.360445804340692	12.913673476829644
11	26.196557679748857	25.151910945212606	30.02275763532754	18.628773739710997
12	25.52518257491124	22.287565015895535	32.68264949926572	19.504602909927502
13	23.07181666594841	23.59326983958602	33.553008880947566	19.781904613518
14	18.78582297112283	28.287923038417805	31.247658764755997	21.678595225703365
15	17.8555445423113	27.035755841899906	35.61851705745267	19.490182558336123
16	20.75188044699775	26.712126686932507	32.30937786956709	20.22661499650265
17	20.464882300095805	26.263189649171075	30.408211975853373	22.863716074879747
18	21.077249989226175	26.549773418153734	31.498854659431075	20.87412193318902
19	21.966919381947044	27.80102898324919	29.237594353852224	20.994457280951544
20	24.10983335377598	27.125344348050934	30.058145509635114	18.706676788537976
21	22.88393771734122	27.346042027866087	30.530204835293063	19.23981541949963
22	21.1702363943154	25.624218897622136	29.68619988927822	23.519344818784248
23	20.478639647016315	26.825914863570215	30.882011688772348	21.813433800641125
24	21.274576754393234	25.45623008914098	31.204563461149583	22.064629695316203
25	20.77367672555253	27.76315484142586	28.949353073192402	22.51381535982921
26	19.689912715435078	27.84578179853277	30.3055291274527	22.158776358579445
27	20.551404409644068	27.234491491992564	30.282655466307762	21.931448632055613
28	18.41139108325018	27.76572398452547	31.38423772695479	22.43864720526956
29	20.10495363939839	25.050719857321397	31.816931150279952	23.027395353000262
30	20.94605793997819	25.02544280424456	31.074117292156323	22.954381963620936
31	20.042714075920664	26.162330063615297	30.233675996247396	23.561279864216644
32	20.773013720881664	26.72969631071051	29.803883218357274	22.693406750050553
33	20.36153644702427	25.706928730312907	29.785070460821395	24.14646436184143
34	18.79402765392482	25.133926943515316	31.65822440719095	24.413820995368912
35	18.390920814037134	24.60708685692691	31.49214173713854	25.509850591897422
36	18.601922050540846	26.239818734522984	30.674739853542267	24.483519361393903
37	18.889831828865233	26.385016757443058	30.600566205988923	24.12458520770279
38	18.594960501496733	25.597864461955133	30.657833234435138	25.149341802112996
39	20.40695226697872	24.169172271818656	29.799905190332066	25.62397027087056
40	20.72113360538625	23.69040002386817	32.5115942941818	23.076872076563777
41	17.95201172192258	25.71720530271136	30.911183894290534	25.41959908107553
42	19.85549813198434	25.259234826309353	30.909112004694073	23.976155037012237
43	20.87503356461146	24.421859927003187	29.87631647864959	24.82679002973576
44	20.33683952303444	25.116688822072753	29.691006673142013	24.855464981750796
45	20.150286583768985	23.56973317377021	30.14673950877984	26.133240733680967
46	20.487673085656887	25.030995468363077	29.91327898905048	24.56805245692956
47	18.552113824641893	24.716316876452392	32.42333179737251	24.3082375015332
48	18.99632695412339	23.8957657206695	31.352993631840135	25.75491369336697
49	19.28531411503794	23.289447949160802	32.12357081055636	25.301667125244897
50	18.219368355450065	23.652360130877124	31.51186612609686	26.616405387575952
51	18.33539417285195	22.995571128798602	30.252903131702563	28.416131566646886
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	576.0
1	1218.5
2	1861.0
3	6438.5
4	11016.0
5	7394.0
6	3772.0
7	3682.5
8	3593.0
9	3639.0
10	3685.0
11	3596.5
12	3508.0
13	3640.0
14	3772.0
15	3628.5
16	3485.0
17	3490.5
18	3496.0
19	3631.5
20	3767.0
21	4047.0
22	4327.0
23	5118.0
24	5909.0
25	7984.0
26	12149.0
27	14239.0
28	15730.5
29	17222.0
30	20849.0
31	24476.0
32	28305.0
33	32134.0
34	35703.5
35	39273.0
36	43035.5
37	46798.0
38	49407.5
39	52017.0
40	57352.5
41	62688.0
42	67705.5
43	72723.0
44	78768.0
45	84813.0
46	100230.5
47	115648.0
48	116310.5
49	116973.0
50	111583.0
51	106193.0
52	92594.5
53	78996.0
54	69394.0
55	59792.0
56	55020.5
57	50249.0
58	47570.5
59	44892.0
60	42678.0
61	40464.0
62	36322.0
63	32180.0
64	26241.5
65	20303.0
66	15989.5
67	11676.0
68	9438.5
69	7201.0
70	5899.0
71	4597.0
72	3990.5
73	3384.0
74	2799.0
75	1749.0
76	1284.0
77	1023.0
78	762.0
79	602.0
80	442.0
81	270.0
82	98.0
83	68.0
84	38.0
85	30.0
86	22.0
87	15.0
88	8.0
89	4.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1206628.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.41278826923884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.46098071640013	20.30405714607236
2	11.049563568006585	6.278978202505325
3	4.050054730515134	3.4522004261086696
4	1.9865897894441567	2.2577822026123435
5	1.1677832783358808	1.658998951585749
6	0.8287977412944872	1.412907284485419
7	0.6302313140718413	1.2534640221157172
8	0.502356235308102	1.1418673079632822
9	0.46428600674490433	1.1872494005412024
>10	7.521252760457677	50.567093004112486
>50	0.2802866217654612	4.82092021573103
>100	0.05106700178871805	2.61438906124823
>500	0.003521862191438641	0.6905382691062392
>1k	0.0029348851595322007	1.833124353310283
>5k	2.9348851595322004E-4	0.526430152501834
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6313	0.5231935608986366	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC	3704	0.3069711626118406	TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	3077	0.25500817153256844	TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	2899	0.24025631760575752	TruSeq Adapter, Index 16 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG	2790	0.23122287896518232	No Hit
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	2081	0.17246409000951413	TruSeq Adapter, Index 27 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC	1906	0.15796086283427865	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1635	0.13550157960862835	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	1405	0.11644019532117604	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCC	1371	0.11362242546998744	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3150233543395314E-4	0.0	0.0	0.17992289255677807	0.0
2	3.3150233543395314E-4	0.0	0.0	0.880553078496438	0.0
3	3.3150233543395314E-4	0.0	0.0	1.3002350351558227	0.0
4	3.3150233543395314E-4	0.0	0.0	1.7956652754618656	0.0
5	3.3150233543395314E-4	0.0	0.0	3.3146089764202387	0.0
6	3.3150233543395314E-4	0.0	0.0	4.134580003116122	0.0
7	3.3150233543395314E-4	0.0	0.0	4.988861521529419	0.0
8	3.3150233543395314E-4	0.0	0.0	6.2386253261154225	0.0
9	4.972535031509297E-4	0.0	0.0	6.720546846252532	0.0
10	4.972535031509297E-4	0.0	0.0	8.003709511133506	0.0
11	4.972535031509297E-4	0.0	0.0	9.440109130568825	0.0
12	4.972535031509297E-4	0.0	0.0	10.702801526236753	0.0
13	4.972535031509297E-4	0.0	0.0	11.168147929602164	0.0
14	4.972535031509297E-4	0.0	0.0	11.359590528315271	0.0
15	4.972535031509297E-4	0.0	0.0	11.696396901116168	0.0
16	5.80129087009418E-4	0.0	0.0	12.319704167315859	0.0
17	6.630046708679063E-4	0.0	0.0	13.131304760042035	0.0
18	6.630046708679063E-4	0.0	0.0	14.01633312006683	0.0
19	7.458802547263946E-4	0.0	0.0	14.620993379898362	0.0
20	7.458802547263946E-4	0.0	0.0	15.15612102487262	0.0
21	7.458802547263946E-4	0.0	0.0	15.86387851102411	0.0
22	7.458802547263946E-4	0.0	0.0	16.644815137722645	0.0
23	7.458802547263946E-4	0.0	0.0	17.392187152958492	0.0
24	7.458802547263946E-4	0.0	0.0	17.961873916401743	0.0
25	8.287558385848829E-4	0.0	0.0	18.460950682397556	0.0
26	8.287558385848829E-4	0.0	0.0	18.932098376633064	0.0
27	9.116314224433711E-4	0.0	0.0	19.39454413456343	0.0
28	9.945070063018594E-4	0.0	0.0	19.890886006291915	0.0
29	9.945070063018594E-4	0.0	0.0	20.4047975017984	0.0
30	9.945070063018594E-4	0.0	0.0	21.002413337001958	0.0
31	9.945070063018594E-4	0.0	0.0	21.523949386223425	0.0
32	9.945070063018594E-4	0.0	0.0	22.026838429076733	0.0
33	0.0010773825901603477	0.0	0.0	22.496494362802785	0.0
34	0.001160258174018836	0.0	0.0	22.965487291857972	0.0
35	0.001160258174018836	0.0	0.0	23.48254805955108	0.0
36	0.0012431337578773243	0.0	0.0	23.9836138395595	0.0
37	0.0012431337578773243	0.0	0.0	24.50398963060695	0.0
38	0.0013260093417358126	0.0	0.0	24.986159777495633	0.0
39	0.0013260093417358126	0.0	0.0	25.508524582555683	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACGTA	20	7.0334255E-4	45.000004	20
CGTCCTA	25	3.890922E-5	45.0	36
CGGTCTA	60	0.0	44.999996	31
AGTCGAG	30	2.1655924E-6	44.999996	1
CGTTTTT	3570	0.0	40.39916	1
CTACGAA	275	0.0	39.272728	11
CGCATCG	35	6.248547E-6	38.57143	21
ACGGGCC	140	0.0	38.57143	5
AGTTCGG	105	0.0	38.57143	2
CATCGTT	35	6.248547E-6	38.57143	36
CGAATAT	285	0.0	37.894737	14
GCGATCG	30	1.1399556E-4	37.499996	9
TGTCGCG	30	1.1399556E-4	37.499996	2
TACGAAT	290	0.0	37.24138	12
GCCGATT	85	0.0	37.058826	9
CGTAAGG	180	0.0	36.250004	2
AAATCGT	25	0.0021071502	36.0	10
CTCAACG	50	4.879257E-8	36.0	1
TAGCGAT	25	0.0021071502	36.0	33
ATTAACG	25	0.0021071502	36.0	1
>>END_MODULE