FastQCFastQC Report
Sat 18 Jun 2016
SRR3550606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206583
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92300.7649701678210284No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG14920.12365498270736452No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG14370.11909665559683834No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC12940.10724500510947028No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12920.10707924775999662No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA12880.10674773306104926No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG253.8909206E-545.0000041
GTATACG253.8909206E-545.0000041
AATGCGT207.033427E-445.022
TGCGTTA207.033427E-445.019
AGTAGCG700.041.7857131
ACGATAG556.184564E-1140.9090921
CATCGTT501.0822987E-940.50000436
CGTTTTT45000.039.751
GCCGATT403.4584082E-739.3759
CGAATAT356.2485487E-638.57142614
TTACGCG700.038.5714261
TATAGCG301.13995586E-437.4999961
TACGGGA3100.037.016134
GCATCGT552.750312E-936.8181835
GCGTAAG800.036.56251
TCGCTAT250.002107149736.00000423
TATCGAG504.879257E-836.0000041
CTACGGG2700.035.8333323
TACGCGG1150.035.217392
ACTACGG1350.035.02
Produced by FastQC (version 0.11.5)