##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550606_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1206583
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33280594869976	33.0	31.0	34.0	31.0	34.0
2	32.46937674407811	34.0	31.0	34.0	31.0	34.0
3	32.41699079135045	34.0	31.0	34.0	30.0	34.0
4	35.98467904818815	37.0	35.0	37.0	35.0	37.0
5	36.090496053731904	37.0	35.0	37.0	35.0	37.0
6	36.11880989538225	37.0	36.0	37.0	35.0	37.0
7	36.20040560823416	37.0	37.0	37.0	35.0	37.0
8	36.20106615127182	37.0	37.0	37.0	35.0	37.0
9	38.02200428814263	39.0	39.0	39.0	35.0	39.0
10	37.686716951921255	39.0	38.0	39.0	35.0	39.0
11	37.58694014419232	39.0	37.0	39.0	35.0	39.0
12	37.49015277026114	39.0	37.0	39.0	35.0	39.0
13	37.485656602156666	39.0	37.0	39.0	35.0	39.0
14	38.76495193451259	40.0	38.0	41.0	35.0	41.0
15	38.890357314830396	40.0	38.0	41.0	35.0	41.0
16	38.92139040579885	40.0	38.0	41.0	35.0	41.0
17	38.87491701772692	40.0	38.0	41.0	35.0	41.0
18	38.77537972936798	40.0	38.0	41.0	35.0	41.0
19	38.67425614317457	40.0	38.0	41.0	35.0	41.0
20	38.569392242390286	40.0	37.0	41.0	35.0	41.0
21	38.474335375187614	40.0	37.0	41.0	35.0	41.0
22	38.47864589506068	40.0	37.0	41.0	35.0	41.0
23	38.44976516327513	40.0	37.0	41.0	35.0	41.0
24	38.35851988632361	40.0	37.0	41.0	35.0	41.0
25	38.2693026505429	40.0	37.0	41.0	34.0	41.0
26	38.29151745051936	40.0	37.0	41.0	34.0	41.0
27	38.23203791202097	40.0	37.0	41.0	34.0	41.0
28	38.168339848978476	40.0	36.0	41.0	34.0	41.0
29	38.0952988729329	40.0	36.0	41.0	34.0	41.0
30	37.976264376342115	40.0	36.0	41.0	34.0	41.0
31	37.91607208124099	40.0	36.0	41.0	34.0	41.0
32	37.73325912929322	40.0	36.0	41.0	33.0	41.0
33	37.59470338965492	40.0	36.0	41.0	33.0	41.0
34	37.47855804366546	40.0	36.0	41.0	33.0	41.0
35	37.368250671524464	40.0	35.0	41.0	33.0	41.0
36	37.27054583066395	40.0	35.0	41.0	33.0	41.0
37	37.20902913434053	40.0	35.0	41.0	33.0	41.0
38	37.12037464476128	40.0	35.0	41.0	33.0	41.0
39	37.03476677526535	40.0	35.0	41.0	32.0	41.0
40	36.94796296649298	40.0	35.0	41.0	32.0	41.0
41	36.84389387219942	40.0	35.0	41.0	32.0	41.0
42	36.79092445360162	39.0	35.0	41.0	32.0	41.0
43	36.696571226347466	39.0	35.0	41.0	31.0	41.0
44	36.61446746721941	39.0	35.0	41.0	31.0	41.0
45	36.53801934885541	39.0	35.0	41.0	31.0	41.0
46	36.43157992446437	39.0	35.0	41.0	31.0	41.0
47	36.37949150617902	39.0	35.0	41.0	31.0	41.0
48	36.309505438084244	39.0	35.0	41.0	31.0	41.0
49	36.22930788847514	39.0	35.0	41.0	31.0	41.0
50	36.13673406636759	38.0	35.0	41.0	31.0	41.0
51	35.250638372992164	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	21.0
9	43.0
10	38.0
11	39.0
12	33.0
13	33.0
14	33.0
15	62.0
16	75.0
17	158.0
18	263.0
19	455.0
20	755.0
21	1202.0
22	1861.0
23	2855.0
24	4432.0
25	7222.0
26	10452.0
27	12739.0
28	13168.0
29	13374.0
30	14603.0
31	17592.0
32	22139.0
33	30563.0
34	64353.0
35	92419.0
36	78730.0
37	118115.0
38	210090.0
39	488027.0
40	637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.02277505981768	21.194729247801437	24.861944847557112	13.92055084482377
2	33.06063486722422	24.33044390646976	25.32523663933604	17.28368458696998
3	32.3589011282274	23.39606972748663	28.156703683045425	16.088325461240544
4	29.333332228284338	25.014938881121317	27.84748334760228	17.80424554299207
5	25.71891034433603	30.22535540447694	25.850521679818133	18.205212571368897
6	26.560128892914953	34.93858275808627	24.2401061510066	14.26118219799218
7	79.73061115563537	6.465282537546112	9.70650174915443	4.097604557664082
8	82.21663988304161	5.394490059946146	8.913684346621825	3.475185710390417
9	76.13831787784181	8.014284968377641	10.956726557559653	4.8906705962208985
10	42.43595343213024	25.749575453988662	17.62116655049839	14.19330456338271
11	31.78065661458847	25.74692333639708	24.129628877582395	18.34279117143205
12	27.959535315846484	23.83715003443609	28.14352597376227	20.059788675955158
13	25.45170949698446	23.646611961216095	30.005892673773786	20.89578586802566
14	21.718936865511946	26.149547938268647	30.670662523837976	21.460852672381428
15	22.092802567249826	25.5739555422213	32.19314377875372	20.140098111775153
16	25.131797812500263	24.275163830420286	30.59963549958851	19.993402857490945
17	24.215325427260286	24.885896784556056	28.990463150898034	21.908314637285624
18	24.57402433152133	25.2115270975971	29.026100981034876	21.1883475898467
19	25.92138294671813	25.719407616384448	27.752089992980178	20.60711944391724
20	26.676573430920207	25.80236916979603	27.96599985247596	19.555057546807802
21	25.722059733976028	25.829387617760236	28.955819864858036	19.4927327834057
22	24.922197644090794	24.22054678376871	28.06976395324648	22.787491618894016
23	24.261986121137127	25.362532042967622	28.442220717513838	21.933261118381413
24	23.503397611270838	25.579508413428663	29.347338724314863	21.569755250985633
25	24.28195988174871	25.91815067840339	27.751178327558073	22.04871111228983
26	23.00712010694664	27.43557633416019	28.041170810462273	21.5161327484309
27	23.387781860012947	26.379121867289694	28.64295286772646	21.590143404970895
28	21.8076999261551	26.957117744904412	29.050218675383295	22.184963653557194
29	23.011015404659275	26.625188652583372	28.51067850284647	21.85311743991089
30	23.389025040133998	26.0758687964276	28.219277082471738	22.315829080966665
31	24.551812846691856	26.365281128608643	26.630078494392844	22.45282753030666
32	24.22021526906976	26.907224782712834	27.125941605343357	21.74661834287405
33	23.79886008670767	26.972450299730728	26.465398567690745	22.763291045870858
34	21.902927523427728	27.401015926794926	27.732530625742278	22.963525924035064
35	23.266447480198213	26.45818812298864	27.485220660327553	22.790143736485597
36	22.994191033687695	29.3726167201096	25.862207572956024	21.770984673246684
37	23.397975937005576	28.24356053416964	26.44633647250127	21.91212705632352
38	22.774976939008752	28.193916208002268	25.98461937554234	23.04648747744664
39	22.93717050546875	26.99084936552231	26.832053824726522	23.239926304282424
40	22.867883933388754	26.24577007963812	27.851047130615964	23.035298856357166
41	21.242716000474065	27.24271765804756	27.209649066827563	24.304917274650812
42	21.96715849634878	27.556579199275973	27.289295473249663	23.186966831125584
43	23.050051260460325	25.655342400812874	28.203115740898056	23.091490597828745
44	22.434014071141398	26.537420136037053	27.15279429595809	23.875771496863457
45	22.665162694982442	25.75778044278761	27.58782445965176	23.98923240257819
46	22.224579660081403	27.000048898418093	27.254900823233875	23.520470618266625
47	22.16101171655825	26.773375723012837	28.226487527173845	22.839125033255065
48	21.81590491495405	26.459016909736004	27.96359637090859	23.761481804401356
49	22.25855991672351	26.209137705404434	28.29088425744437	23.24141812042769
50	21.42504908489511	26.299309703518116	28.183224858961214	24.092416352625555
51	21.4484208711709	26.053408675573912	27.233103731778087	25.265066721477098
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	667.0
1	1742.0
2	2817.0
3	9333.0
4	15849.0
5	10530.0
6	5211.0
7	5013.0
8	4815.0
9	4755.0
10	4695.0
11	4847.0
12	4999.0
13	4898.5
14	4798.0
15	4692.0
16	4586.0
17	4365.5
18	4145.0
19	4230.5
20	4316.0
21	4572.5
22	4829.0
23	5088.5
24	5348.0
25	6911.5
26	9886.0
27	11297.0
28	13988.5
29	16680.0
30	19538.5
31	22397.0
32	24526.5
33	26656.0
34	27842.5
35	29029.0
36	31266.5
37	33504.0
38	36885.5
39	40267.0
40	45137.5
41	50008.0
42	54857.5
43	59707.0
44	65143.0
45	70579.0
46	76569.0
47	82559.0
48	88577.5
49	94596.0
50	93203.5
51	91811.0
52	86302.5
53	80794.0
54	74405.0
55	68016.0
56	65742.5
57	63469.0
58	61876.5
59	60284.0
60	58891.0
61	57498.0
62	52823.5
63	48149.0
64	43159.5
65	38170.0
66	33253.5
67	28337.0
68	23657.5
69	18978.0
70	16404.5
71	13831.0
72	11806.5
73	9782.0
74	8269.5
75	5191.5
76	3626.0
77	2970.0
78	2314.0
79	1670.5
80	1027.0
81	849.5
82	672.0
83	404.0
84	136.0
85	98.5
86	61.0
87	44.0
88	27.0
89	18.0
90	9.0
91	5.5
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1206583.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.63354674567948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.35795448652344	17.824330542655236
2	9.023943565378332	4.445834712962415
3	3.1430765615828546	2.322753702150024
4	1.6468914975903848	1.6227511476381933
5	1.0591847887230217	1.30457390026606
6	0.7986188025950192	1.180368816342179
7	0.6843966134385048	1.1801381178805497
8	0.5815764933072081	1.146103338725717
9	0.5256466222945174	1.165368657780047
>10	9.678447947943706	56.495551177589455
>50	0.4371787837848351	6.484198044131727
>100	0.05596146816287328	2.5166099303182343
>500	0.0037307645441755945	0.5833268220383477
>1k	0.00305244371796185	0.9569500744238377
>5k	3.391604131068722E-4	0.771141015097959
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9230	0.7649701678210284	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1492	0.12365498270736452	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1437	0.11909665559683834	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	1294	0.10724500510947028	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1292	0.10707924775999662	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1288	0.10674773306104926	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4863602421051846E-4	0.0	0.0	0.0925754796810497	0.0
2	3.3151469894735793E-4	0.0	0.0	0.34494104425472594	0.0
3	3.3151469894735793E-4	0.0	0.0	0.49188493456314236	0.0
4	3.3151469894735793E-4	0.0	0.0	0.6743008976589261	0.0
5	3.3151469894735793E-4	0.0	0.0	1.1790320268062786	0.0
6	4.1439337368419745E-4	0.0	0.0	1.6380970061736324	0.0
7	4.1439337368419745E-4	0.0	0.0	2.0460258432283562	0.0
8	4.1439337368419745E-4	0.0	0.0	2.697452226659915	0.0
9	4.1439337368419745E-4	0.0	0.0	3.0198502713862205	0.0
10	4.1439337368419745E-4	0.0	0.0	3.5993379651462023	0.0
11	4.1439337368419745E-4	0.0	0.0	4.382707198758809	0.0
12	4.972720484210369E-4	0.0	0.0	5.000070446873527	0.0
13	4.972720484210369E-4	0.0	0.0	5.257823125305097	0.0
14	4.972720484210369E-4	0.0	0.0	5.375179328732462	0.0
15	4.972720484210369E-4	0.0	0.0	5.55038484712614	0.0
16	4.972720484210369E-4	0.0	0.0	5.87924742848192	0.0
17	4.972720484210369E-4	0.0	0.0	6.2790541554124335	0.0
18	4.972720484210369E-4	0.0	0.0	6.706459481030315	0.0
19	4.972720484210369E-4	0.0	0.0	6.993385452969253	0.0
20	4.972720484210369E-4	0.0	0.0	7.280145667558718	0.0
21	4.972720484210369E-4	0.0	0.0	7.640004873266075	0.0
22	4.972720484210369E-4	0.0	0.0	8.012958909581853	0.0
23	4.972720484210369E-4	0.0	0.0	8.396770052288156	0.0
24	4.972720484210369E-4	0.0	0.0	8.72811899388604	0.0
25	6.630293978947159E-4	0.0	0.0	9.028471311132346	0.0
26	6.630293978947159E-4	0.0	0.0	9.30976153318918	0.0
27	6.630293978947159E-4	0.0	0.0	9.578785711384961	0.0
28	7.459080726315554E-4	0.0	0.0	9.868529558264951	0.0
29	8.287867473683949E-4	0.0	0.0	10.166312636594416	0.0
30	8.287867473683949E-4	0.0	0.0	10.504954901569143	0.0
31	8.287867473683949E-4	0.0	0.0	10.81707599062808	0.0
32	8.287867473683949E-4	0.0	0.0	11.118174215947016	0.0
33	8.287867473683949E-4	0.0	0.0	11.433444694645955	0.0
34	8.287867473683949E-4	0.0	0.0	11.735537464061734	0.0
35	8.287867473683949E-4	0.0	0.0	12.055863541919619	0.0
36	8.287867473683949E-4	0.0	0.0	12.378095829296452	0.0
37	8.287867473683949E-4	0.0	0.0	12.696681454984862	0.0
38	8.287867473683949E-4	0.0	0.0	13.02347206947222	0.0
39	8.287867473683949E-4	0.0	0.0	13.36410342264063	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	25	3.8909206E-5	45.000004	1
GTATACG	25	3.8909206E-5	45.000004	1
AATGCGT	20	7.033427E-4	45.0	22
TGCGTTA	20	7.033427E-4	45.0	19
AGTAGCG	70	0.0	41.785713	1
ACGATAG	55	6.184564E-11	40.909092	1
CATCGTT	50	1.0822987E-9	40.500004	36
CGTTTTT	4500	0.0	39.75	1
GCCGATT	40	3.4584082E-7	39.375	9
CGAATAT	35	6.2485487E-6	38.571426	14
TTACGCG	70	0.0	38.571426	1
TATAGCG	30	1.13995586E-4	37.499996	1
TACGGGA	310	0.0	37.01613	4
GCATCGT	55	2.750312E-9	36.81818	35
GCGTAAG	80	0.0	36.5625	1
TCGCTAT	25	0.0021071497	36.000004	23
TATCGAG	50	4.879257E-8	36.000004	1
CTACGGG	270	0.0	35.833332	3
TACGCGG	115	0.0	35.21739	2
ACTACGG	135	0.0	35.0	2
>>END_MODULE